7,935 results

1. pV1464

   Plasmid

   #111440

   Purpose

   N. castellii Solo CRISPR vector, marked with URA3 and NatR

   Depositor

   Gerald Fink

   Article

   Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

   Insert

   CaCas9/sgRNA-BsmBI stuffer
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMJA149

   Plasmid

   #128524

   Purpose

   Expresses mutant Ber e 1 in Pichia

   Depositor

   Marcos Alcocer

   Insert

   mutant Ber e 1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   AOX1

   Available since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCfB2226

   Plasmid

   #67533

   Purpose

   EasyClone system-based yeast integrative vector carrying loxP-flanked SpHIS5 marker, integration into S. cerevisiae X-4 chromosomal location, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

   Type

   Empty backbone

   Use

   Cre/Lox; Integrative

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUCdeltaSS-ERE

   Plasmid

   #108217

   Purpose

   Yeast reporter gene with estrogen response element (ERE) upstream of CYC1 promoter driving lacZ expression

   Depositor

   Didier Picard, Keith Yamamoto

   Article

   Picard et al Nature 1990 Nov 8;348(6297):166-8.

   Insert

   ERE-CYC1-lacZ
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   CYC1

   Available since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCfB2197

   Plasmid

   #67551

   Purpose

   EasyClone system-based yeast integrative vector carrying loxP-flanked natMX marker, integration into S. cerevisiae XII-1 chromosomal location, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

   Type

   Empty backbone

   Use

   Cre/Lox; Integrative

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGAL - FFL[SEL]

   Plasmid

   #1102

   Depositor

   Susan Lindquist

   Article

   Singer et al Mol Cell 1998 Apr;1(5):639-48.

   Insert

   firefly luciferase
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   n/a

   Promoter

   Available since

   Jan. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

7. pEZYpci

   Plasmid

   #18707

   Purpose

   pPIC3.5K converted Gateway Destionation vector for multiple copy integration of gene of interest into Pichia

   Depositor

   Yu-Zhu Zhang

   Article

   Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 26, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBS4

   Plasmid

   #83793

   Purpose

   Useful for tagging yeast proteins with C-terminal CFP tag; hygromycin-B selection

   Depositor

   Eric Muller

   Article

   Hailey et al Methods Enzymol. 2002;351:34-49.

   Insert

   CFP
   

   Use

   Tags

   CFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCfB2899(X-2 MarkerFree)

   Plasmid

   #73271

   Purpose

   EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-2 (Chr X: 194944..195980)

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. NM R(delta) 2-5 GFP

    Plasmid

    #1209

    Depositor

    Susan Lindquist

    Article

    Liu et al Nature 1999 Aug 5;400(6744):573-6.

    Insert

    sup35 NM domain (SUP35 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    last four repeats of sup35 N domain deleted

    Promoter

    Available since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJT42_GalL_BE-PAPAPA

    Plasmid

    #145035

    Purpose

    Expresses BE-PAPAPA in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

    Insert

    BE-PAPAP
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A)

    Promoter

    GalL

    Available since

    July 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGBW-m4046310

    Plasmid

    #149022

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 M (membrane) (M Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;MBP

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pGBW-m4046423

    Plasmid

    #148998

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp7

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp7 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;MBP

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pETCON-DnvLB16

    Plasmid

    #45124

    Depositor

    David Baker

    Article

    Procko et al J Mol Biol. 2013 Sep 23;425(18):3563-75.

    Insert

    DnvLB16
    

    Use

    Tags

    Aga2p for yeast surface display and c-myc epitope…

    Expression

    Yeast

    Mutation

    Promoter

    GAL

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. prs316 CUP-GFP

    Plasmid

    #128052

    Purpose

    Yeast expression vector for N terminal GFP fusions

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDblet

    Plasmid

    #8848

    Depositor

    Joel Huberman

    Article

    Brun et al Gene. 1995 Oct 16. 164(1):173-7.

    Insert

    ARS doublet
    

    Use

    Yeast cloning vector

    Tags

    Expression

    Yeast

    Mutation

    This vector contains, between AatII sites, a dire…

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGP8GF-AtTIR1

    Plasmid

    #188427

    Purpose

    Arabidopsis Nuclear Auxin Signaling Receptor TIR1

    Depositor

    Britney Moss

    Article

    Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

    Insert

    Auxin Signaling Receptor TIR1 (TIR1 Mustard Weed)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MtPT4

    Plasmid

    #117096

    Purpose

    protein expression in yeast

    Depositor

    Geoffrey Chang

    Insert

    MtPT4
    

    Use

    Tags

    His

    Expression

    Yeast

    Mutation

    Promoter

    AOX

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJAS1052

    Plasmid

    #158598

    Purpose

    VioA, VioB

    Depositor

    John Dueber

    Article

    Grewal et al Nat Chem Biol. 2020 Oct 12. pii: 10.1038

    Inserts

    VioA
    VioB
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. YIplac211-APL2-iGFPx6

    Plasmid

    #105251

    Purpose

    Plasmid for pop-in/pop-out replacement of Apl2 with Apl2-iGFPx6.

    Depositor

    Benjamin Glick

    Article

    Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

    Insert

    APL2 portion (APL2 Budding Yeast)
    

    Use

    Tags

    iGFPx6

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBS-SKII-URA-GAL1-HALO-GS

    Plasmid

    #188940

    Purpose

    Integration cassette to create N-terminal fusions for fluorescent tagging

    Depositor

    Stephen Buratowski

    Article

    Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

    Insert

    pBS-SKII-URA-GAL1-HALO-GS
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRS406-PHO5-GFP-2xOsh2 PH domain [short]

    Plasmid

    #58829

    Purpose

    Expresses GFP-Tagged Osh2 PH domain in yeast

    Depositor

    Tim Levine

    Article

    Roy et al J Biol Chem. 2004 Oct 22;279(43):44683-9

    Insert

    2xOsh2 PH domain [short] (OSH2 Budding Yeast)
    

    Use

    Tags

    GFP and Myc

    Expression

    Yeast

    Mutation

    Promoter

    PHO5

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

23. pV1329

    Plasmid

    #111438

    Purpose

    Solo vector pV1326 +sgCgADE2

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgCgADE2
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. 413-Met25 Kar2 TorsinA GFP

    Plasmid

    #32119

    Depositor

    Susan Lindquist

    Article

    Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

    Insert

    TorsinA (TOR1A Human)
    

    Use

    Tags

    GFP and Kar2

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

25. LD113

    Plasmid

    #69166

    Purpose

    Integration of GFP tag at Atg13 C terminus, use auxotrophic marker URA3(Candida albicans)

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    Atg13-EGFP
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCR

    Plasmid

    #60619

    Purpose

    crRNA expression plasmid on pRS423 backbone

    Depositor

    Huimin Zhao

    Article

    Bao et al ACS Synth Biol. 2014 Sep 19.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHES841

    Plasmid

    #89194

    Purpose

    Expresses YFP under control of synthetic promoter containing Zif268 binding sites.

    Depositor

    Hana El-Samad

    Article

    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

    Insert

    pZ-YFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pZ

    Available since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRS-Dhh1-PA (405)

    Plasmid

    #121417

    Purpose

    Expressing Dhh1-PA

    Depositor

    Daniel Klionsky

    Article

    Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

    Insert

    Dhh1promoter-Dhh1 ORF-PA-ADH1 terminator (DHH1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pFA6a-link-yomApple-SpHis5

    Plasmid

    #44844

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mApple with SpHis5 selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomApple
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pILGFPQ4

    Plasmid

    #83559

    Purpose

    Used to evaluate the expression output of GAL7 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

    Insert

    GAL7 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL7

    Available since

    Dec. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pL-rpb1-N488D

    Plasmid

    #10988

    Depositor

    Jeff Strathern

    Article

    Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

    Insert

    rpb1-N488D (RPO21 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Mutation 1462 A to G in the RPB1 ORF resulting in…

    Promoter

    Available since

    Dec. 16, 2005

    Availability

    Academic Institutions and Nonprofits only

32. pH3FS-PYK1

    Plasmid

    #85781

    Purpose

    N-ICE plasmid pH3FS-PYK1

    Depositor

    Scott Briggs

    Article

    Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

    Insert

    loxP-HphMX-loxP-PYK1p-3FLAG
    

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDA200

    Plasmid

    #74248

    Purpose

    pGPD1-dPSTRy(4,3)

    Depositor

    Serge Pelet

    Article

    Aymoz et al Nat Commun. 2016 Apr 21;7:11304. doi: 10

    Insert

    pGPD1-dPSTRy(4,3)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pRG213MX

    Plasmid

    #64522

    Purpose

    Centromeric E. coli/S. cerevisiae shuttle vector

    Depositor

    Joerg Stelling

    Article

    Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pTPI1-EMC1-GFP-APEX2-URA3

    Plasmid

    #184776

    Purpose

    Expresses EMC1-GFP-APEX2 chimera for labeling yeast endoplasmic reticulum (ER). Uses auxotrophic marker URA3(Kluyveromyces lactis).

    Depositor

    Zhiping Xie

    Article

    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

    Insert

    APEX2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pPpT4- TEF2prom-TIR1F74G-FLAG

    Plasmid

    #189727

    Purpose

    Enables expression of OsTIR1(F74G) from the TEF2 promoter, used to implement the AID2 system in Komagataella phaffii (original name pLB219)

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

    Insert

    OsTIR1
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    TEF2

    Available since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pPpT4- HTA1prom-TIR1 F74G -FLAG

    Plasmid

    #189728

    Purpose

    Enables expression of OsTIR1(F74G) from the HTA1 promoter, used to implement the AID2 system in Komagataella phaffii (original name pLB220)

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

    Insert

    OsTIR1
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    HTA1

    Available since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAG423GAL-EGFP-ccdB

    Plasmid

    #14317

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAG424GAL-EGFP-ccdB

    Plasmid

    #14319

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCfB2227

    Plasmid

    #67536

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked SpHIS5 marker, integration into S. cerevisiae XI-3 chromosomal location, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge