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Yeast Expression

8,021 results

Showing: 2161 - 2200 of 8021 results

  1. MtPT4-eGFP

    Plasmid
    #117095
    Purpose
    protein expression in yeast
    Depositor
    Geoffrey Chang
    Insert
    MtPT4-eGFP
    Use
    Tags
    His
    Expression
    Yeast
    Mutation
    Promoter
    AOX
    Available since
    Nov. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    117095-plasmid-map-sequence-id-224993

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  6. pDBD-R

    Plasmid
    #50571
    Purpose
    Double Ub R-ek Beta-gal construct for ubiquitin sandwich assay, for measurement of cotranslational protein degradation, use with 50572
    Depositor
    Alexander Varshavsky
    Article
    Turner et al Science. 2000 Sep 22;289(5487):2117-20.
    Insert
    DHFR-Ubiquitin-ArgBetaGal-Ubiquitin-DHFR-Ubiquitin
    Use
    Tags
    Expression
    Yeast
    Mutation
    M1R (B-gal)
    Promoter
    Gal1
    Available since
    Feb. 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  8. pICE LYS2

    Plasmid
    #122879
    Purpose
    LYS2 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.
    Depositor
    Randy Hampton
    Article
    Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340
    Type
    Empty backbone
    Use
    Yeast recombination cloning
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    122879-plasmid-map-sequence-id-238638

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  11. pEVA1

    Plasmid
    #58953
    Purpose
    Contains a synthetic DNA sequence, VAR1u, that specifies the Var1 protein. The Cox4 targeting signal is fused to the N-terminus to direct import of Var1. Complements var1 mutations in mtDNA.
    Depositor
    Thomas Fox, Thomas Mason
    Article
    Sanchirico et al EMBO J. 1998 Oct 1;17(19):5796-804.
    Insert
    VAR1u, VAR1 recoded for expression from the nucleus
    Use
    Yeast ecoli shuttle vector
    Tags
    preCox4 mitochondrial matrix targeting signal
    Expression
    Yeast
    Mutation
    Synthetic gene in standard genetic code
    Promoter
    ADH1
    Available since
    Aug. 15, 2014
    Availability
    Academic Institutions and Nonprofits only

  12. pAV10.K5

    Plasmid
    #63210
    Purpose
    pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI or BciVI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Feb. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    63210-plasmid-map-sequence-id-104911

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  13. pAV10.K4

    Plasmid
    #63212
    Purpose
    pCACS backbone (Amp), TRP1, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into chrIXR: 387328-388330.
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Feb. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    63212-plasmid-map-sequence-id-104913

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  14. TEF2pro_mCherry_YFP

    Plasmid
    #138623
    Purpose
    Dual reporter backbone plasmid for CCRA experiment
    Depositor
    Rob Mitra
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    138623-plasmid-map-sequence-id-274912

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  15. p404MET25

    Plasmid
    #17420
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    MET25 promoter + polylinker + CYC1 terminator (MET17 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    MET25 promoter is between SacI and XbaI restrictiā€¦
    Promoter
    Available since
    March 12, 2008
    Availability
    Academic Institutions and Nonprofits only
    17420-plasmid-map-sequence-id-8595

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  21. Coexpressed Free GFP-NES

    Plasmid
    #182491
    Purpose
    Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and fusion protein GFP-AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.
    Inserts
    FPPS
    GFP-NES
    Use
    Synthetic Biology; Metabolic engineering
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10 and GAL7
    Available since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182491-plasmid-map-sequence-id-360449

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  24. pAV11K

    Plasmid
    #63184
    Purpose
    pRS416 backbone, URA3 replaced by KanMX, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63184-plasmid-map-sequence-id-104886

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  29. pBridge-tyrosinase.Ļƒ1A

    Plasmid
    #197381
    Purpose
    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-1 Ļƒ1A fusion protein in yeast (yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Inserts
    tyrosinase cytosolic tail
    AP-1 Ļƒ1A
    Use
    Tags
    GAL4-DNA binding domain fragment, HA tag, and SV4ā€¦
    Expression
    Yeast
    Mutation
    Contains an extra 42 nucleotides encoding 14 resiā€¦
    Promoter
    ADH1 and MET25
    Available since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197381-plasmid-map-sequence-id-388878

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  36. pEZYpci

    Plasmid
    #18707
    Purpose
    pPIC3.5K converted Gateway Destionation vector for multiple copy integration of gene of interest into Pichia
    Depositor
    Yu-Zhu Zhang
    Article
    Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    July 26, 2008
    Availability
    Academic Institutions and Nonprofits only
    18707-plasmid-map-sequence-id-8835

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  39. Padh1-OsTIR1(WT)-NLS-TADH1-Sp.arg3+/pUC19*

    Plasmid
    #169352
    Purpose
    Expression of OsTIR1(WT) in S. pombe from adh1 promoter, integrated at arg3-D4 locus
    Depositor
    Tony Carr
    Insert
    OsTIR1(WT)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    169352-plasmid-map-sequence-id-332958

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Showing: 2161 - 2200 of 8021 results