8,265 results
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Plasmid#44639PurposeYeast tagging vector to create gene fusion with mDsRed with SpHis5 selectionDepositorInsertmDsRed
ExpressionYeastAvailable SinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pXP214
Plasmid#26829DepositorTypeEmpty backboneUseCre/LoxExpressionYeastAvailable SinceDec. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pTH734-2µ-RLuc/stopCFLuc
Plasmid#40609DepositorInsertsFirefly Luciferase (nonsense mutant)
Renilla luciferase
ExpressionYeastMutationAll codons of the original FLuc gene were exchang…PromoterADH1 and TDH3Available SinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6-mTFP1-His3MX6
Plasmid#69850PurposeC-terminal tagging with mTFP1 using His3MX6 as a selection marker in S. cerevisiae.DepositorInsertmTFP1
ExpressionYeastAvailable SinceApril 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMZ284
Plasmid#52225PurposeExpression of sgRNA precursor targeted against ade6+DepositorInsertrrk1-sgRNA
UseCRISPRExpressionYeastPromoterrrk1Available SinceOct. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFBP-4_1.sensor
Plasmid#162844PurposeYeast expression of the RNA-based sensor for fructose-1,6-bisphosphate #4_1DepositorInsert4_1_RNA-device
ExpressionYeastAvailable SinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFBP-4_1m.mutant
Plasmid#162869PurposeYeast expression of the mutant #4_1m for the RNA-based sensor of fructose-1,6-bisphosphate #4_1DepositorInsert4_1m_RNA-device
ExpressionYeastAvailable SinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETCON-HtsptLB12
Plasmid#45120DepositorInsertHtsptLB12
TagsAga2p for yeast surface display and c-myc epitope…ExpressionYeastAvailable SinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNRB143
Plasmid#36452DepositorAvailable SinceAug. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
YN2_1_IL50_HOlocus
Plasmid#194426PurposeExpresses Cas9 and sgRNA cassettes for targeting the HO locus in yeast Saccharomyces cerevisiaeDepositorInsertCas9
ExpressionYeastMutationNAAvailable SinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG426Gal-GFP
Plasmid#38310DepositorInsertEGFP (eGFP )
ExpressionYeastAvailable SinceNov. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAG413GPD-ccdB-ECFP
Plasmid#14166DepositorTypeEmpty backboneUseGateway destinationTagsECFPExpressionYeastAvailable SinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBS5
Plasmid#83799PurposeUseful for tagging yeast proteins with N-terminal CFP tag; G418 selectionDepositorInsertCFP
ExpressionYeastAvailable SinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSEYC68-HA-TOR2 (pAS21)
Plasmid#39207DepositorAvailable SinceJuly 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMOD8A-iRGR-r11
Plasmid#74369PurposeThis plasmid constitutively expresses gRNA-r11 from an AHD1 promoter using the insulated RGR design. Integrates in the W303 HIS locus and restores HIS function.DepositorArticleInsertRGR-r11
UseCRISPR and Synthetic BiologyExpressionYeastPromoterAHD1Available SinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P3nmt1-3FLAG
Plasmid#19336DepositorInsert3FLAG
ExpressionYeastAvailable SinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pKT0146
Plasmid#8742DepositorInserttdimer2
TagsCodon optimized linkerExpressionYeastAvailable SinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoPSmOrange-SpHis5
Plasmid#44856PurposeYeast tagging vector to create gene fusion with yeast optimized photoconvertible mOrange with SpHis5 selectionDepositorInsertyoPSmOrange
ExpressionYeastAvailable SinceMay 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
Cip-pAct1-Mnase
Plasmid#162556PurposeNheI-MluI-digested 3xFLAG-MNase-SV40 fragment was cloned into the CIp-pACT1-CYC vector to ensure constitutive expression of MNase in C. albicans.DepositorInsert3FLAG-Mnase-SV40
ExpressionYeastPromoterActineAvailable SinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046310
Plasmid#149022PurposeYeast Expression plasmid for SARS-CoV-2 M (membrane)DepositorInsertSARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
TagsCleavable thrombin;MBPExpressionYeastMutationwtPromoterpTEF1Available SinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046423
Plasmid#148998PurposeYeast Expression plasmid for SARS-CoV-2 nsp7DepositorInsertSARS-CoV-2 nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
TagsCleavable thrombin;MBPExpressionYeastMutationwtPromoterpTEF1Available SinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDRF'- AmTryoshka1;3-LS-F138I-T78H
Plasmid#100963PurposeYeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFPDepositorInsertAmTryoshka1;3-LS-F138I-T78H (AMT1;3 Mustard Weed)
ExpressionYeastMutationF138I, T78HPromoterPMAAvailable SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
nuc-yHS1-M7A
Plasmid#159167PurposeNuclear labile heme reporterDepositorInsertnuc-yHS1-M7A
TagsSV40 NLSExpressionYeastMutationMutated Met 7 of the cyt b562 module of nuc-yHS1 …PromoterGPDAvailable SinceSept. 21, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGP8GF-AtTIR1
Plasmid#188427PurposeArabidopsis Nuclear Auxin Signaling Receptor TIR1DepositorInsertAuxin Signaling Receptor TIR1 (TIR1 Mustard Weed)
ExpressionYeastAvailable SinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDIV494
Plasmid#177701PurposePlasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hanseniiDepositorInsertsCas9
Nat
sgRNA Targeting ADE2 locus in D.hansenii: AGCTAAGCAGATTAATGCAT
UseCRISPRTagsSV40ExpressionBacterial and YeastPromoterRNR2p (Debaryomyces hansenii ), SNR52p (Candida s…Available SinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJAS1668
Plasmid#158593PurposeVioE-ePTS1 CEN6/ARS4DepositorInsertVioE-ePTS1
ExpressionYeastAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-URA-GAL1-HALO-GS
Plasmid#188940PurposeIntegration cassette to create N-terminal fusions for fluorescent taggingDepositorInsertpBS-SKII-URA-GAL1-HALO-GS
ExpressionYeastAvailable SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pJW1510
Plasmid#62364PurposepFA6a pSEC63-BirA-mVenus-UBC6-TA::HIS5DepositorInsertBirA-mVenus-Ubc6 tail anchor
ExpressionYeastMutationL232H mutation compared to standard mVenusPromoterSec63Available SinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGAD424-hSyntenin2b
Plasmid#89427PurposeTwo-hybrid systemDepositorAvailable SinceJuly 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAG415GAL-ccdB-EYFP
Plasmid#14217DepositorTypeEmpty backboneUseGateway destinationTagsEYFPExpressionYeastAvailable SinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
cyterm-ymScarletI pDRF1-GW
Plasmid#168082PurposeExpresses Cyterm-ymScarletI to check for monomericity.DepositorInsertcyterm-ymScarletI
ExpressionYeastAvailable SinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJW1660
Plasmid#112034PurposeC-terminal tag with AID*(AtIAA17_71-114)-HA marked with LEUDepositorInsertIAA17
TagsHAExpressionYeastMutationonly amino acids 71-114PromoterNoneAvailable SinceAug. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
HsSPHK2
Plasmid#118092PurposeExpresses sphingosine kinase 2, which catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P)DepositorAvailable SinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFPQ4
Plasmid#83559PurposeUsed to evaluate the expression output of GAL7 promoter with yEGFP used as the reporter gene.DepositorInsertGAL7 promoter-yEGFP
ExpressionYeastPromoterGAL7Available SinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTPI1-EMC1-GFP-APEX2-URA3
Plasmid#184776PurposeExpresses EMC1-GFP-APEX2 chimera for labeling yeast endoplasmic reticulum (ER). Uses auxotrophic marker URA3(Kluyveromyces lactis).DepositorInsertAPEX2
ExpressionYeastAvailable SinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPpT4- TEF2prom-TIR1F74G-FLAG
Plasmid#189727PurposeEnables expression of OsTIR1(F74G) from the TEF2 promoter, used to implement the AID2 system in Komagataella phaffii (original name pLB219)DepositorInsertOsTIR1
TagsFLAGExpressionYeastPromoterTEF2Available SinceSept. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPpT4- HTA1prom-TIR1 F74G -FLAG
Plasmid#189728PurposeEnables expression of OsTIR1(F74G) from the HTA1 promoter, used to implement the AID2 system in Komagataella phaffii (original name pLB220)DepositorInsertOsTIR1
TagsFLAGExpressionYeastPromoterHTA1Available SinceSept. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYIAL31
Plasmid#60807Purposeused to make mutations in yeast POL1DepositorAvailable SinceFeb. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAG303GAL-EGFP-ccdB
Plasmid#14301DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
myc ATG18
Plasmid#42530DepositorAvailable SinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pZMU-Dest
Plasmid#105089PurposeYeast expression vector for Gateway cloning and SUS bridge assaysDepositorTypeEmpty backboneTagsmycExpressionYeastAvailable SinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pBS10
Plasmid#83795PurposeUseful for tagging yeast proteins with C-terminal Cerulean tag; hygromycin-B selectionDepositorInsertCerulean
TagsCeruleanExpressionYeastAvailable SinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046464
Plasmid#148970PurposeYeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
TagsCleavable thrombin;3xFLAGExpressionYeastMutationwtPromoterpGAL1Available SinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYTK096
Plasmid#65203PurposePre-Assembled Ura3 integration vector to be used in the Dueber YTK system.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCoofy42
Plasmid#55190PurposePichia pastoris expression vector for parallel SLIC cloning. C-terminal tags can be added during SLIC.DepositorTypeEmpty backboneTagsHis10-Stop-OneStrep-Stop-S-tag-Stop-CBP-HPC4-Stop…ExpressionYeastPromoterAOX1Available SinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFTK056
Plasmid#171328PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertSpCas9-NLS
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG303GAL-ccdB-ECFP
Plasmid#14157DepositorTypeEmpty backboneUseGateway destinationTagsECFPExpressionYeastAvailable SinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRS314-hUD
Plasmid#11005DepositorInserthUD recombination system
ExpressionBacterial and YeastMutationDirect repeat recombination system. Recombination…Available SinceApril 28, 2006AvailabilityAcademic Institutions and Nonprofits only