Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Yeast Expression Clear filter

Applied Filters

Yeast Expression Clear filter

8,350 results

Showing: 2701 - 2750 of 8350 results

  5. pGBW-m4046299

    Plasmid
    #153878
    Purpose
    Yeast expression plasmid for SARS-CoV-2 S (surface glycoprotein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable thrombin;6xHIS
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pGAL1
    Available Since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153878-plasmid-map-sequence-id-300422

    Enlarge

  6. pFA6a-PATagRFP-KanMX6

    Plasmid
    #87027
    Purpose
    Tag S. pombe genes in C terminal with PATagRFP
    Depositor
    Julien Berro
    Insert
    PATagRFP
    Expression
    Bacterial and Yeast
    Available Since
    Feb. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    87027-plasmid-map-sequence-id-172998

    Enlarge

  7. pGBW-m4046337

    Plasmid
    #145566
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 nsp4
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable thrombin;6xHIS
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pGAL1
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145566-plasmid-map-sequence-id-298841

    Enlarge

  20. MtPT4-eGFP

    Plasmid
    #117095
    Purpose
    protein expression in yeast
    Depositor
    Geoffrey Chang
    Insert
    MtPT4-eGFP
    Tags
    His
    Expression
    Yeast
    Promoter
    AOX
    Available Since
    Nov. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    117095-plasmid-map-sequence-id-224993

    Enlarge

  24. pFA6a-link-mDsRed-SpHis5

    Plasmid
    #44639
    Purpose
    Yeast tagging vector to create gene fusion with mDsRed with SpHis5 selection
    Depositor
    Kurt Thorn
    Insert
    mDsRed
    Expression
    Yeast
    Available Since
    May 7, 2013
    Availability
    Academic Institutions and Nonprofits only
    44639-plasmid-map-sequence-id-61107

    Enlarge

  25. pFA0055

    Plasmid
    #131774
    Purpose
    Guide RNA (gCASS5a) and Cas9 expression plasmid for cleaving pFA6 series deletion cassettes, including KanMX, hphMX and natMX. Used to perform CRISPR-Swap of alleles.
    Depositor
    Frank Albert
    Article
    Lutz et al PLoS Genet. 2019 Nov 18;15(11):e1008375.
    Insert
    20mer CASS5a guide (gCASS5a) and 5' sgRNA
    Expression
    Bacterial and Yeast
    Available Since
    Oct. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    131774-plasmid-map-sequence-id-258731

    Enlarge

  26. pIAeBlue

    Plasmid
    #185891
    Purpose
    In vivo gene amplification (HapAmp) of AeBlue expression module at RPL25 locus
    Depositor
    Claudia Vickers
    Article
    Peng et al Nat Commun. 2022 May 24;13(1):2895. doi:
    Insert
    PRPL25(Arm 1)> Kl.LEU2>TKl.LEU2- TRPL25(Arm 3)- ARS305- PALD6>AeBlue>TPGK1- PBTS1> RPL25(partial; Arm2)
    Expression
    Yeast
    Mutation
    None
    Available Since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185891-plasmid-map-sequence-id-370863

    Enlarge

  28. pAV10.K5

    Plasmid
    #63210
    Purpose
    pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI or BciVI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    Feb. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    63210-plasmid-map-sequence-id-104911

    Enlarge

  29. pAV10.K4

    Plasmid
    #63212
    Purpose
    pCACS backbone (Amp), TRP1, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into chrIXR: 387328-388330.
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    Feb. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    63212-plasmid-map-sequence-id-104913

    Enlarge

  33. p406GALS

    Plasmid
    #17422
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    p406GALS (GAL1 Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    GALS promoter is between SacI and XbaI restrictio…
    Available Since
    March 12, 2008
    Availability
    Academic Institutions and Nonprofits only
    17422-plasmid-map-sequence-id-8597

    Enlarge

  39. Coexpressed Free GFP-NES

    Plasmid
    #182491
    Purpose
    Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and fusion protein GFP-AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.
    Inserts
    FPPS
    GFP-NES
    Use
    Synthetic Biology; Metabolic engineering
    Expression
    Yeast
    Promoter
    GAL10 and GAL7
    Available Since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182491-plasmid-map-sequence-id-360449

    Enlarge

  43. pBridge-tyrosinase.σ1A

    Plasmid
    #197381
    Purpose
    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-1 σ1A fusion protein in yeast (yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Inserts
    tyrosinase cytosolic tail
    AP-1 σ1A
    Tags
    GAL4-DNA binding domain fragment, HA tag, and SV4…
    Expression
    Yeast
    Mutation
    Contains an extra 42 nucleotides encoding 14 resi…
    Promoter
    ADH1 and MET25
    Available Since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197381-plasmid-map-sequence-id-388878

    Enlarge

  50. Padh1-OsTIR1(WT)-NLS-TADH1-Sp.arg3+/pUC19*

    Plasmid
    #169352
    Purpose
    Expression of OsTIR1(WT) in S. pombe from adh1 promoter, integrated at arg3-D4 locus
    Depositor
    Tony Carr
    Insert
    OsTIR1(WT)
    Expression
    Yeast
    Available Since
    May 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    169352-plasmid-map-sequence-id-332958

    Enlarge

Showing: 2701 - 2750 of 8350 results