8,173 results
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Plasmid#169149PurposeEncodes OptoQ-AMP3DepositorInsertVP16-EL222
TagsSV40 NLSExpressionYeastMutationA79QAvailable SinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMAL716
Plasmid#169150PurposeEncodes OptoQ-AMP4DepositorInsertVP16-EL222
TagsSV40 NLSExpressionYeastAvailable SinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pESC-LEU-PmCDA1
Plasmid#60818Purposeto overexpress lamprey deaminase in yeastDepositorInsertCDA1
TagsFLAGExpressionYeastPromoterGAL10Available SinceMarch 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAG304GPD-ccdB
Plasmid#14136DepositorTypeEmpty backboneUseGateway destinationExpressionYeastAvailable SinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
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YIp LYS2-HIS3
Plasmid#122908PurposeLYS2-HIS3 integrating (YIp) vector for stable genomic integration in the BY4741 background.DepositorTypeEmpty backboneUseYeast recombination cloningExpressionYeastAvailable SinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD153
Plasmid#212929PurposeEncodes the mRuby2 fluorescent protein (Part3b') with the 111AA SCW4 TFP (Part3a') assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.DepositorInsertmRuby2
ExpressionYeastAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD141
Plasmid#212928PurposeEncodes the mRuby2 fluorescent protein (Part3b') with the Aga2 signal peptide (Part3a') assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.DepositorInsertmRuby2
ExpressionYeastAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS313_ATP3
Plasmid#120253PurposeExpression of ATP3 in yeastDepositorAvailable SinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS313_ATP3T911A
Plasmid#120254PurposeExpression of ATP3-6 in yeastDepositorInsertATP3 (ATP3 Budding Yeast)
ExpressionYeastMutationT911 changed to A; Isoleucine 304 changed to Aspa…PromoterATP3Available SinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS414 GPD-Sis1
Plasmid#18687DepositorInsertSis1 (SIS1 Budding Yeast)
ExpressionYeastAvailable SinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
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FRP2000
Plasmid#159309PurposeYeast tagging vector to create gene fusion with yeast codon optimized mNeptune2 under KanMX selectionDepositorTypeEmpty backboneExpressionYeastAvailable SinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS426-TUB1
Plasmid#60385PurposeExpression of yeast alpha tubulin (TUB1) using a GAL promoterDepositorAvailable SinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pK3F
Plasmid#85770PurposeN-ICE plasmid pK3FDepositorInsertloxP-KanMX-loxP-3FLAG
UseCre/LoxExpressionYeastAvailable SinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAG416-GAL-TDP-43
Plasmid#181709PurposeGalactose inducible expression of TDP-43DepositorInsertTDP-43 (TARDBP Human)
ExpressionYeastAvailable SinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pILAC3
Plasmid#185890PurposeIn vivo gene amplification (HamAmp) of lycopene synthetic module at RPL25 locusDepositorInsertsee comments
ExpressionYeastMutationERG20 F96C; Xd.CRtYB E83KAvailable SinceSept. 1, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pIT6EG7ml
Plasmid#185886PurposeIn vivo gene amplification (HamAmp) of limonene synthetic module at RPL25 locus (14 copy).DepositorInsertsee comments
ExpressionYeastMutationERG20 F96W N127WAvailable SinceSept. 1, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKT0175
Plasmid#8733DepositorInsertyECitrine
TagsCodon optimized linkerExpressionYeastMutationyEGFP with S65G, V68L, Q69M, S72A, T203YAvailable SinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCfB6578
Plasmid#106130PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntC_2, USER site for cloning, amp resistanceDepositorTypeEmpty backboneExpressionYeastAvailable SinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-FKBP(M)x4
Plasmid#85004PurposeExpresses mEGFP-FKBP(M)x4DepositorInsertEGFP-FKBP(M)x4
ExpressionYeastAvailable SinceDec. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
3019_pETcon-SARS-CoV-2-RBD_E484K
Plasmid#184404Purposeyeast surface display of the SARS-CoV-2 Eta variant RBDDepositorInsertSARS-CoV-2 Eta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
TagsHA and c-MycExpressionYeastMutationE484KAvailable SinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX-tetO-Pcyc1-GFP-LINKER
Plasmid#52687PurposeSame as 41025, with additional linkerDepositorInserttetO7-Pcyc1-GFP+LINKER
UsePromoter replacementExpressionYeastAvailable SinceMay 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yEPAGFP-CaUra3
Plasmid#44649DepositorInsertyEPAGFP
ExpressionYeastAvailable SinceMay 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046451
Plasmid#145567PurposeYeast Expression plasmid for SARS-CoV-2 nsp6DepositorInsertSARS-CoV-2 nsp6 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
TagsCleavable thrombin;6xHISExpressionYeastMutationwtPromoterpGAL1Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFA6a-6xGLY-S-tag-kanMX6
Plasmid#20766DepositorTypeEmpty backboneUsePcr-based c-terminal taggingExpressionYeastAvailable SinceMarch 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
APEX2 VLP
Plasmid#182211PurposeYeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and APEX2 tagged with VP2C (GAL10 promoter).DepositorInsertsVP2C-APEX2
Murine polyomavirus deltaVP1 (NLS deletion mutant)
UseSynthetic Biology; Tem imagingExpressionYeastPromoterGAL1 and GAL10Available SinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKT0176
Plasmid#8743DepositorInserttdimer2
TagsCodon optimized linkerExpressionYeastAvailable SinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pPMVAugw
Plasmid#98300PurposeEnhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of CUP1 promoter.DepositorInsertTEFM1EfmvaE>TEBS1
ExpressionYeastAvailable SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS406-PHO5-GFP-2xOsh2 PH domain [short]
Plasmid#58829PurposeExpresses GFP-Tagged Osh2 PH domain in yeastDepositorInsert2xOsh2 PH domain [short] (OSH2 Budding Yeast)
TagsGFP and MycExpressionYeastPromoterPHO5Available SinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEZYpci
Plasmid#18707PurposepPIC3.5K converted Gateway Destionation vector for multiple copy integration of gene of interest into PichiaDepositorTypeEmpty backboneExpressionYeastAvailable SinceJuly 26, 2008AvailabilityAcademic Institutions and Nonprofits only -
pILGFP97
Plasmid#83551PurposeUsed to evaluate the expression output of CYC1 promoter with yEGFP used as the reporter gene.DepositorInsertCYC1 promoter-yEGFP
ExpressionYeastPromoterCYC1Available SinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYTSG
Plasmid#159419PurposeExpression of proteins with a C-terminal thermostable GFP (TGP) tag in Saccharomyces cerevisiaeDepositorInsertthermostable GFP (TGP)
Tagspolyhistidine (poly-His)ExpressionYeastAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGAL-SKI1-NAT
Plasmid#160382PurposeOverxpression of SKI1/XRN1 in yeastDepositorInsertSKI1
ExpressionYeastPromoterGALAvailable SinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
LD202
Plasmid#69179PurposeIntegration of 2GFP tag at Atg2 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg2-2EGFP
Tags2xEGFPExpressionYeastAvailable SinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
413-Met25 Kar2 TorsinA GFP
Plasmid#32119DepositorAvailable SinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHES841
Plasmid#89194PurposeExpresses YFP under control of synthetic promoter containing Zif268 binding sites.DepositorInsertpZ-YFP
ExpressionYeastPromoterpZAvailable SinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
NM R(delta) 2-5 GFP
Plasmid#1209DepositorInsertsup35 NM domain (SUP35 Budding Yeast)
TagsGFPExpressionYeastMutationlast four repeats of sup35 N domain deletedAvailable SinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
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pAG306-pTet07-synYFP[TTG/AGA]-nLuc
Plasmid#199756PurposeElongation reporter construct to quantify elongation of synYFP[TTG]; Control construct of elongation reporter to quantify elongation duration of n_CGA stallingDepositorInsertsynYFP[TTG/AGA]-nLuc
ExpressionYeastPromoterTetO7Available SinceMay 25, 2023AvailabilityAcademic Institutions and Nonprofits only