8,025 results
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Plasmid#153022PurposeSingle-plasmid multi-gene expression system suitable for fungi. The obtained polycistronic gene is expressed under the control of a TetON inducible promoterDepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceOct. 8, 2020AvailabilityAcademic Institutions and Nonprofits only
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pFA6a-link-yoPA-mCherry-SpHis5
Plasmid#44855PurposeYeast tagging vector to create gene fusion with yeast optimized photoactivatable mCherry with SpHis5 selectionDepositorInsertyoPA-mCherry
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
LD113
Plasmid#69166PurposeIntegration of GFP tag at Atg13 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg13-EGFP
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCfB2803
Plasmid#63646PurposepCfB2803 is a vector for multiple integrations at sites sharing homology with Ty4Cons. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoternoneAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pV1464
Plasmid#111440PurposeN. castellii Solo CRISPR vector, marked with URA3 and NatRDepositorInsertCaCas9/sgRNA-BsmBI stuffer
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomRuby-SpHis5
Plasmid#44843PurposeYeast tagging vector to create gene fusion with yeast optimized mRuby with SpHis5 selectionDepositorInsertyomRuby
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pETCON-DnvLB16
Plasmid#45124DepositorInsertDnvLB16
UseTagsAga2p for yeast surface display and c-myc epitope…ExpressionYeastMutationPromoterGALAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCfB2226
Plasmid#67533PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked SpHIS5 marker, integration into S. cerevisiae X-4 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXP216
Plasmid#26830DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceDec. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P81nmt1-mYFP
Plasmid#105144PurposeYeast gene targetingDepositorInsertkanMX6-P81nmt1-mYFP
UseTags81nmt1-mYFPExpressionBacterial and YeastMutationA206KPromoter81nmt1Available sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046494
Plasmid#149137PurposeYeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 surface glycoprotein (S Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;6xHISExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpTEF1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pV1329
Plasmid#111438PurposeSolo vector pV1326 +sgCgADE2DepositorInsertCaCas9/sgCgADE2
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceDec. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG424GAL-EGFP-ccdB
Plasmid#14319DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastMutationPromoterAvailable sinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
HCP5
Plasmid#166107PurposeThis plasmid encodes a Cas9 protein as well as a sgRNAs that targets the C-terminus of Cyr1.DepositorInsertCyr1CT-sgRNA (CYR1 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJT42_GalL_BE-PAPAPA
Plasmid#145035PurposeExpresses BE-PAPAPA in yeast cellsDepositorInsertBE-PAPAP
UseCRISPRTagsExpressionYeastMutationspCas9(D10A)PromoterGalLAvailable sinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDR-GW FLIPglu-170nΔ13V
Plasmid#27999DepositorInsertFLIPglu-170nΔ13V
UseTagseCFP and eYFPExpressionYeastMutationWT binding protein (no F16A)PromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTH734-2µ-RLuc/stopCFLuc
Plasmid#40609DepositorInsertsFirefly Luciferase (nonsense mutant)
Renilla luciferase
UseTagsExpressionYeastMutationAll codons of the original FLuc gene were exchang…PromoterADH1 and TDH3Available sinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTH760-CEN-mCherry_v3
Plasmid#40598DepositorInsertmCherry (Red Fluorescent Protein variant) with yeast promoter and terminator sequences
UseTagsExpressionYeastMutationmCherry ORF partially codon optimised for yeast e…PromoterGPD (TDH3)Available sinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHES609 [prNOp7-PBAC]
Plasmid#103017PurposeA S. cerevisiae plasmid. A photosensitive adenylate cyclase that produces cAMP in response to blue light. Used to activate PKA.DepositorInsertbPAC
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
MtPT4
Plasmid#117096Purposeprotein expression in yeastDepositorInsertMtPT4
UseTagsHisExpressionYeastMutationPromoterAOXAvailable sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDA200
Plasmid#74248PurposepGPD1-dPSTRy(4,3)DepositorInsertpGPD1-dPSTRy(4,3)
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRPR1(1xTetO)_gRNA_handle_RPR1t
Plasmid#62966PurposeaTc inducible gRNA expression vectorDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterRPR1Available sinceMarch 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pH3FS-PYK1
Plasmid#85781PurposeN-ICE plasmid pH3FS-PYK1DepositorInsertloxP-HphMX-loxP-PYK1p-3FLAG
UseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMEL15
Plasmid#107921Purposenat based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS415-KEX2
Plasmid#10996DepositorInsertKEX2 (KEX2 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationWild typePromoterAvailable sinceDec. 29, 2005AvailabilityAcademic Institutions and Nonprofits only -
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YIplac211-APL5-mCherry2Bx6
Plasmid#105253PurposePlasmid for pop-in/pop-out replacement of Apl5 with Apl5-mCherry2Bx6.DepositorInsertApl5 (APL5 Budding Yeast)
UseTagsmCherry2Bx6ExpressionYeastMutationPromoterAvailable sinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW mScarletI-T2A-mTurquoise2
Plasmid#118430PurposeProduces equimolar expression of mScarletI and mTq2DepositorInsertmScarletI-T2A-mTurquoise2
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG413GPD-EGFP-ccdB
Plasmid#14310DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastMutationPromoterAvailable sinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
YN2_1_IL50_HOlocus
Plasmid#194426PurposeExpresses Cas9 and sgRNA cassettes for targeting the HO locus in yeast Saccharomyces cerevisiaeDepositorInsertCas9
UseTagsExpressionYeastMutationNAPromoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDH3
Plasmid#83789PurposeUseful for tagging yeast proteins with C-terminal CFP tag; G418 selectionDepositorInsertCFP
UseTagsCFPExpressionYeastMutationF64L, Y66W, N146I, M153T, V163A, N164H mutations …PromoterAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pAW
Plasmid#64745PurposeYeast expression plasmid: ACC1DepositorInsertACC1
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS-Dhh1-PA (405)
Plasmid#121417PurposeExpressing Dhh1-PADepositorInsertDhh1promoter-Dhh1 ORF-PA-ADH1 terminator (DHH1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
RPL3-GFP (96)
Plasmid#24040DepositorInsertRPL3 (RPL3 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceMarch 31, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCfB2227
Plasmid#67536PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked SpHIS5 marker, integration into S. cerevisiae XI-3 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046310
Plasmid#149022PurposeYeast Expression plasmid for SARS-CoV-2 M (membrane)DepositorInsertSARS-CoV-2 M (membrane) (M Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;MBPExpressionYeastMutationwtPromoterpTEF1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046423
Plasmid#148998PurposeYeast Expression plasmid for SARS-CoV-2 nsp7DepositorInsertSARS-CoV-2 nsp7 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;MBPExpressionYeastMutationwtPromoterpTEF1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMJB1
Plasmid#160429PurposeYeast vector for expressing mCherry tagged genes under the GAL1 promoterDepositorTypeEmpty backboneUseTagsmCherryExpressionYeastMutationPromoterGAL1Available sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDR-GW FLIPglu-30μΔ13V
Plasmid#27998DepositorInsertFLIPglu-30μΔ13V
UseTagseCFP and eYFPExpressionYeastMutationD154A in mglBPromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only