7,935 results

1. pAG423GAL-ccdB-EYFP

   Plasmid

   #14221

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   EYFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pXRLy2

   Plasmid

   #63149

   Purpose

   Lys2 marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.

   Depositor

   Marc Gartenberg

   Article

   Chou et al FEMS Yeast Res. 2015 May;15(3):fov010. d

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p413GAL1-Zipp.-L-IFP-F[2] (Yeast)

   Plasmid

   #52893

   Purpose

   Plasmid+insert encoding GCN4-leucine zipper fused to IFP-F[2] by the linker (GGGGS) X 2. Zipper-L-IFP-F[2] (XbaI/XhoI). Zipper is (XbaI /ClaI). Plasmid confers ampicillin resistance to DH5α.

   Depositor

   Stephen Michnick

   Article

   Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

   Insert

   Zipp.-L-IFP-F[2]
   

   Use

   Tags

   L-IFP-F[2]

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   June 5, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pFA6a-kanMX6-3nmt1-mMaple3

   Plasmid

   #105167

   Purpose

   Yeast gene targeting

   Depositor

   Jian-Qiu Wu

   Article

   Liu et al Mol Biol Cell. 2016 Aug 15;27(16):2528-4

   Insert

   kanMX6-3nmt1-mMaple3
   

   Use

   Tags

   3nmt1-mMaple3

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   3nmt1

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAG423GPD-ccdB-DsRed

   Plasmid

   #14366

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   DsRed

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 29, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pNIA-CEN-FLAG-LINXa4(RLR)-Bre1

   Plasmid

   #116884

   Purpose

   FLAG-tagged LINXa4-Bre1 with RLR hinge-loop mutations

   Depositor

   Brian Kuhlman

   Article

   Lerner et al ACS Synth Biol. 2018 Nov 29. doi: 10.102

   Insert

   LINXa4(RLR)-Bre1
   

   Use

   Synthetic Biology

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Bre1 point mutations K8A, K9A and K11A, LINXa3 wi…

   Promoter

   ADH1

   Available since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. YN2_1_LT84_RPL13A

   Plasmid

   #177712

   Purpose

   Expresses Cas9 and sgRNA cassette for targeting RPL13A in yeast Saccharomyces cerevisiae

   Depositor

   Lisbeth Olsson

   Article

   Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFA6a-6xGLY-VSV-G-hphMX4

   Plasmid

   #20773

   Depositor

   Mark Hochstrasser

   Article

   Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

   Type

   Empty backbone

   Use

   Pcr-based c-terminal tagging

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGB-REL

   Plasmid

   #27277

   Depositor

   Thomas Gilmore

   Article

   Leeman et al Oncogene. 2008 Dec 4. 27(53):6770-81.

   Insert

   REL (REL Human)
   

   Use

   Tags

   GAL4 (aa1-147)

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 1, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pRG221

    Plasmid

    #64520

    Purpose

    Episomal E. coli/S. cerevisiae shuttle vector

    Depositor

    Joerg Stelling

    Article

    Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAV0756

    Plasmid

    #133508

    Purpose

    Expression of 3mCherry tagged CRIB domain to monitor active Cdc42

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pHis5(StuI)-p(act1)-CRIB(gic2aa2-181)-3mCherry-bsdMX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGY416

    Plasmid

    #166660

    Purpose

    For light-inducible Cre recombinase (LiCre) expression under the Gal1 promoter in yeast

    Depositor

    Gaël Yvert

    Article

    Duplus-Bottin et al Elife. 2021 Feb 23;10. pii: 61268. doi:

    Insert

    LiCre
    

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Cre: E340A, D341A

    Promoter

    GAL1

    Available since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pILGFP4L

    Plasmid

    #104507

    Purpose

    Used to evaluate the expression output of Saccharomyces mikatae GAL2 promoter with yEGFP used as the reporter gene

    Depositor

    Claudia Vickers

    Article

    Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

    Insert

    SmGAL2 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LHP424-YCplac33-STE2-WT

    Plasmid

    #32171

    Depositor

    Linda Hicke

    Insert

    STE2 (STE2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    STE2

    Available since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pXP618

    Plasmid

    #26848

    Depositor

    Suzanne Sandmeyer, Nancy DaSilva

    Article

    Fang et al Yeast. 2010 Sep 10. ():.

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNB784

    Plasmid

    #60163

    Purpose

    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by SIC1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc-yEVenus
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    FLuc has A4V & S504G (no functional effect)

    Promoter

    SIC1pr

    Available since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAG426GAL-ECFP-ccdB

    Plasmid

    #14299

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    ECFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRS DV DNM2-mRUBY2

    Plasmid

    #107793

    Purpose

    donor vector for knock-in gene editing at human DNM2 locus

    Depositor

    Sandra Schmid

    Article

    Srinivasan et al PLoS Biol. 2018 Apr 18;16(4):e2005377. d

    Insert

    DNM2-mRuby2 (DNM2 Synthetic, Human)
    

    Use

    Yeast-e.coli shuttle vector

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRS DV DNM1-EGFP

    Plasmid

    #107794

    Purpose

    donor vector for knock-in gene editing at human DNM1 locus

    Depositor

    Sandra Schmid

    Article

    Srinivasan et al PLoS Biol. 2018 Apr 18;16(4):e2005377. d

    Insert

    DNM1-eGFP (DNM1 Synthetic, Human)
    

    Use

    Yeast-e.coli shuttle vector

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBBK11 pCAS-Tyr-[gRNA: 1=FF18] (SplitKanR, AmpR)

    Plasmid

    #178995

    Purpose

    Sp.Cas9 and gRNA yeast expression vector with FF18 gRNA pre-cloned. Selection =SplitKanamycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. POM42-mCitrine

    Plasmid

    #69854

    Purpose

    N-terminal tagging with mCitrine using URA3 as a selection marker in S. cerevisiae.

    Depositor

    Liza Pon

    Article

    Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

    Insert

    mCitrine minus start and stop codon
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. nuc-yHS1-M7A,H102A

    Plasmid

    #159168

    Purpose

    Nuclear labile heme reporter

    Depositor

    Amit Reddi

    Article

    Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

    Insert

    nuc-yHS1-M7A,H102A
    

    Use

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Mutated both Met 7 and His 102 of the cyt b562 mo…

    Promoter

    GPD

    Available since

    Sept. 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. mito-yHS1-M7A,H102A

    Plasmid

    #159165

    Purpose

    Mitochondrial labile heme reporter

    Depositor

    Amit Reddi

    Article

    Martinez-Guzman et al J Cell Sci. 2020 May 20;133(10). pii: jc

    Insert

    mito-yHS1-M7A,H102A
    

    Use

    Tags

    COX4 pre-sequence

    Expression

    Yeast

    Mutation

    Mutated both Met 7 and His 102 of the cyt b562 mo…

    Promoter

    GPD

    Available since

    Sept. 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. P413TEF-PYK1

    Plasmid

    #34605

    Depositor

    Markus Ralser

    Article

    Gruning et al Cell Metab. 2011 Sep 7;14(3):415-27.

    Insert

    PYK1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available since

    Jan. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pKT-SCFP3A-HIS

    Plasmid

    #64686

    Purpose

    pKT vector encoding yeast-codon optimized and fast-folding cyan fluorescent protein SCFP3A.

    Depositor

    Erin O'Shea

    Article

    Hansen et al Mol Syst Biol. 2013 Nov 5;9:704. doi: 10

    Insert

    SCFP3A
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Nucleotide sequence has been yeast-codon optimize…

    Promoter

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBBK49 pHomL-RPL18Bp-Neon(gfp)-Link-TUB1-SSA1t-HomR (AmpR)

    Plasmid

    #179033

    Purpose

    Vector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-TUB1, a marker for the microtubules

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    RPL18Bp-Neon(gfp)-Link-TUB1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. LD231

    Plasmid

    #69193

    Purpose

    Integration of 2GFP tag at Atg31 C terminus, use auxotrophic marker URA3(Candida albicans)

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    Atg31-2EGFP
    

    Use

    Tags

    2xEGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pXP412

    Plasmid

    #26840

    Depositor

    Suzanne Sandmeyer, Nancy DaSilva

    Article

    Fang et al Yeast. 2010 Sep 10. ():.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. YIplac211-vps10-104

    Plasmid

    #115426

    Purpose

    Generate the vps10-104 allele via pop-in pop-out mutagenesis

    Depositor

    Benjamin Glick

    Article

    Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

    Insert

    vps10-104
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    domain 1 deletion

    Promoter

    Endogenous

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. 416Gal-FUS-R521H-YFP

    Plasmid

    #29624

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    R521H

    Promoter

    Available since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

31. pAG416GPD-ccdB-TAP

    Plasmid

    #14268

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    TAP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pIEforRed

    Plasmid

    #185892

    Purpose

    In vivo gene amplification (HamAmp) of EforRed expression module at RPL25 locus

    Depositor

    Claudia Vickers

    Insert

    PRPL25(Arm 1)> Kl.LEU2>TKl.LEU2- TRPL25(Arm 3)- ARS305- PALD6>EforRed>TPGK1-PBTS1> RPL25(partial; Arm2)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pGBW-m4046327

    Plasmid

    #153913

    Purpose

    Yeast expression plasmid for SARS-CoV-2 nsp4

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;StrepII

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. pAV0764

    Plasmid

    #133520

    Purpose

    Expression of mCherry targeted to the endoplasmic reticulum with a signal sequence and a retention signal (AHDL)

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pLys3(BstZ17I)-p(BiP)-SignalSequenceBiP-mCherry-AHDL-bsdMX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. UASGAL-CU2-Pleum

    Plasmid

    #60328

    Purpose

    Contains a promoter comprised of derivatives from native promoters galactose, cyc and leucine.

    Depositor

    Hal Alper

    Article

    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

    Insert

    promoter
    

    Use

    Synthetic Biology; Yeast synthetic hybrid promote…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 6, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. FRP1671

    Plasmid

    #64538

    Purpose

    Inducible MET15 gene

    Depositor

    Joerg Stelling

    Article

    Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

    Insert

    MET15 (MET17 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    (lexA-box)_4-P_CYC1_minimal

    Available since

    Aug. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pYC44

    Plasmid

    #63903

    Purpose

    Initial Integrative Vector

    Depositor

    Irene Castano Navarro

    Article

    Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

    Type

    Empty backbone

    Use

    Tags

    FRT::3'UTRcta::NAT::FRT

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAG425GAL-ccdB-DsRed

    Plasmid

    #14369

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    DsRed

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. FRP1432

    Plasmid

    #64509

    Purpose

    Expresses destabilized Citrine in yeast.

    Depositor

    Joerg Stelling

    Article

    Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

    Insert

    Citrine-ADH1-tail
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    Aug. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. GPD 104QDProGFP p416

    Plasmid

    #15569

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt 104Q Delta Pro (HTT Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Proline-rich region removed

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only