8,025 results

1. pDZ536 pURA ADE3p PP7-PS-2x-yeGFP

   Plasmid

   #72234

   Purpose

   Uracil selection; Expression of PP7-2x-yeGFP fusion protein under the ADE3 promoter

   Depositor

   Daniel Zenklusen

   Article

   Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

   Insert

   PP7-PS-2x-yeGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ADE3p

   Available since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGBDU-Atg17

   Plasmid

   #121394

   Purpose

   For Yeast-two-hybrid analysis

   Depositor

   Daniel Klionsky

   Article

   Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

   Insert

   ATG17
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ADH1 promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. GPD 25QDProGFP p416

   Plasmid

   #15568

   Depositor

   Susan Lindquist

   Article

   Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

   Insert

   htt 25Q Delta Pro (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Proline-rich region removed

   Promoter

   Available since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pRS315e_pGal-dCas9-SH3

   Plasmid

   #79613

   Purpose

   Expresses dCas9-SH3 in yeast cells

   Depositor

   Akihiko Kondo

   Article

   Nishida et al Science. 2016 Aug 4. pii: aaf8729.

   Insert

   SpCas9
   

   Use

   Tags

   SH3 domain

   Expression

   Yeast

   Mutation

   D10A and H840A for nuclease deficient Cas9

   Promoter

   pGal1

   Available since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDN-T2dGZmxh

   Plasmid

   #44552

   Depositor

   Gabor Balazsi

   Article

   Nevozhay et al PLoS Comput Biol. 2012;8(4):e1002480. do

   Inserts

   PTETREG promoter
   yEGFP::ZeoR
   

   Use

   Synthetic Biology; Expression regulator/reporter

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PTETREG

   Available since

   April 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TSG101

   Plasmid

   #31412

   Depositor

   Rachel Klevit

   Article

   Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

   Insert

   Tumor susceptibility gene 101 (TSG101 Human)
   

   Use

   Tags

   HA

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pSK226

   Plasmid

   #129268

   Purpose

   dual yTRAP, RNQ1 fusion to aTF2 reporting on [RNQ+] state with mNeonGreen reporter

   Depositor

   Ahmad Khalil

   Article

   Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

   Insert

   Rnq1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHB4

   Plasmid

   #53462

   Purpose

   This plasmid contains an ADHpr driven chimeric protein with SNC1, N-terminal GFP and C-terminal SUC2. It contains flanking homology to SUC2, allowing optional integration.

   Depositor

   Elizabeth Conibear

   Article

   Burston et al J Cell Biol. 2009 Jun 15. 185(6):1097-11

   Insert

   yeGFP-Snc1-Suc2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ADH

   Available since

   May 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. HisSumoOphP

   Plasmid

   #189672

   Purpose

   HisSUMO tagged OphP to express OphP in pichia pastorias.

   Depositor

   Markus Künzler, James Naismith

   Article

   Emmanuel et al bioRxiv 2022.07.21.500988

   Insert

   HisSumoOphP
   

   Use

   Tags

   HisSumo tagged

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCfB3047(gRNA XII-1)

    Plasmid

    #73289

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-1

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSec7-Halox2

    Plasmid

    #115425

    Purpose

    Express Sec7-Halox2 at the endogenous locus via pop-in pop-out mutagenesis

    Depositor

    Benjamin Glick

    Article

    Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

    Insert

    Sec7-Halox2
    

    Use

    Tags

    HaloTag

    Expression

    Yeast

    Mutation

    Promoter

    Endogenous

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZEN

    Plasmid

    #129113

    Purpose

    Z. rouxii episomal vector containing ClonNAT resistance

    Depositor

    Paolo Giudici

    Article

    Bizzarri et al Yeast. 2019 Dec;36(12):711-722. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pZCAG

    Plasmid

    #129114

    Purpose

    Z. rouxii centromeric vector containing G418 resistance

    Depositor

    Paolo Giudici

    Article

    Bizzarri et al Yeast. 2019 Dec;36(12):711-722. doi: 10.

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBKWH-Lam

    Plasmid

    #133899

    Purpose

    Negative control. Expression of Gal4BD-Lam hybrid protein. Homology regions for recombination with pAWH

    Depositor

    Sebastian Maurer

    Article

    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

    Insert

    LMNA (Lam) (LMNA Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    T7

    Available since

    June 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CUP Rnq1 YFP p316

    Plasmid

    #15596

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    RNQ1 (RNQ1 Budding Yeast)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

16. 416Gal-FUS-H517Q-YFP

    Plasmid

    #29620

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    H517Q

    Promoter

    Available since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

17. Ube2d2

    Plasmid

    #31410

    Depositor

    Rachel Klevit

    Article

    Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

    Insert

    Ubiquitin Conjugating Enzyme (UBE2D2 Human)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pHLK

    Plasmid

    #64182

    Purpose

    harbours marker genes HIS3, LEU2 and LYS2

    Depositor

    Markus Ralser

    Article

    Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRS406-PHO5-GFP-CERT PH domain

    Plasmid

    #58725

    Purpose

    Expresses GFP-Tagged CERT PH domain in yeast

    Depositor

    Tim Levine

    Article

    Levine et al Curr Biol. 2002 Apr 30;12(9):695-704.

    Insert

    collagen, type IV, alpha 3 binding protein PH domain (CERT1 Human)
    

    Use

    Tags

    GFP and Myc

    Expression

    Yeast

    Mutation

    Promoter

    PHO5

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pRS426-TUB1-internal6xHis

    Plasmid

    #60390

    Purpose

    Expression of yeast alpha tubulin (TUB1) - internal 6xHis using a GAL promoter

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    TUB1 (TUB1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    internal 6xHis (see supplement figure 4)

    Promoter

    GAL

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

21. pCfB2802

    Plasmid

    #63655

    Purpose

    pCfB2802 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

    Depositor

    Irina Borodina, Jerome Maury

    Article

    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

    Insert

    GFP
    

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 promoter

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDIV490

    Plasmid

    #177698

    Purpose

    Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.

    Depositor

    Uffe Mortensen

    Article

    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

    Inserts

    Cas9
    Nat
    

    Use

    CRISPR

    Tags

    SV40

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    RHR2p (Debaryomyces hansenii ) and TDH3p (Candida…

    Available since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSB305-K3L-vac-HA

    Plasmid

    #20054

    Depositor

    Harmit Malik

    Article

    Elde et al Nature. 2009 Jan 22;457(7228):485-9. doi

    Insert

    K3L
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

24. pAG423GAL-ccdB-EYFP

    Plasmid

    #14221

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EYFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJK369

    Plasmid

    #73594

    Purpose

    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope

    Depositor

    T. J. Kappock

    Insert

    delta-12 fatty acid acetylenase
    

    Use

    Tags

    FLAG tag

    Expression

    Yeast

    Mutation

    Promoter

    GAL10

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. p413GAL1-Zipp.-L-IFP-F[2] (Yeast)

    Plasmid

    #52893

    Purpose

    Plasmid+insert encoding GCN4-leucine zipper fused to IFP-F[2] by the linker (GGGGS) X 2. Zipper-L-IFP-F[2] (XbaI/XhoI). Zipper is (XbaI /ClaI). Plasmid confers ampicillin resistance to DH5α.

    Depositor

    Stephen Michnick

    Article

    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

    Insert

    Zipp.-L-IFP-F[2]
    

    Use

    Tags

    L-IFP-F[2]

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

27. pFA6a-6xGLY-VSV-G-hphMX4

    Plasmid

    #20773

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAG423GPD-ccdB-DsRed

    Plasmid

    #14366

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    DsRed

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 29, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pPS2169

    Plasmid

    #1364

    Depositor

    Pamela Silver

    Article

    Stage-Zimmermann et al Mol Biol Cell 2000 Nov;11(11):3777-89.

    Insert

    RPL11B (RPL11B Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 3, 2005

    Availability

    Academic Institutions and Nonprofits only

30. pXP618

    Plasmid

    #26848

    Depositor

    Nancy DaSilva, Suzanne Sandmeyer

    Article

    Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pXP314

    Plasmid

    #26835

    Depositor

    Nancy DaSilva, Suzanne Sandmeyer

    Article

    Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pBTM116-D9

    Plasmid

    #111232

    Purpose

    yeast expression vector

    Depositor

    Ulrich Stelzl

    Article

    Worseck et al Methods Mol Biol. 2012;812:63-87. doi: 1

    Insert

    ccdB-cassette
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAG414GAL-EGFP-ccdB

    Plasmid

    #14311

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAG426GAL-ECFP-ccdB

    Plasmid

    #14299

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    ECFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. LHP424-YCplac33-STE2-WT

    Plasmid

    #32171

    Depositor

    Linda Hicke

    Insert

    STE2 (STE2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    STE2

    Available since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

36. YIplac211-APL5-iGFPx6

    Plasmid

    #105252

    Purpose

    Plasmid for pop-in/pop-out replacement of Apl5 with Apl5-iGFPx6.

    Depositor

    Benjamin Glick

    Article

    Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

    Insert

    Apl5 (APL5 Budding Yeast)
    

    Use

    Tags

    iGFPx6

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pILGFP4I

    Plasmid

    #104504

    Purpose

    Used to evaluate the expression output of Saccharomyces kudriavzevii GAL1 promoter with yEGFP used as the reporter gene

    Depositor

    Claudia Vickers

    Article

    Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

    Insert

    SkGAL1 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pILGFP4Q

    Plasmid

    #104512

    Purpose

    Used to evaluate the expression output of Saccharomyces kudriavzevii GAL2 promoter with yEGFP used as the reporter gene

    Depositor

    Claudia Vickers

    Article

    Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

    Insert

    SkGAL2 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pKT-SCFP3A-HIS

    Plasmid

    #64686

    Purpose

    pKT vector encoding yeast-codon optimized and fast-folding cyan fluorescent protein SCFP3A.

    Depositor

    Erin O'Shea

    Article

    Hansen et al Mol Syst Biol. 2013 Nov 5;9:704. doi: 10

    Insert

    SCFP3A
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Nucleotide sequence has been yeast-codon optimize…

    Promoter

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. LD231

    Plasmid

    #69193

    Purpose

    Integration of 2GFP tag at Atg31 C terminus, use auxotrophic marker URA3(Candida albicans)

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    Atg31-2EGFP
    

    Use

    Tags

    2xEGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge