8,265 results

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pACT2 V1H (P#663)

Plasmid

#14665

Depositor

Matija Peterlin

Article

Geyer et al Mol Biol Cell. 2002 Jun . 13(6):2045-56.

Insert

V1H (ATP6V1H Human)

Tags

Gal4 AD

Expression

Yeast

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
pDS247

Plasmid

#34967

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2(SS/TM)-GFP-LOVpep

Expression

Yeast

Mutation

T406A, T407A, V416I, T–2S

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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pDS248

Plasmid

#34968

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2(SS/TM)-GFP-LOVpep

Expression

Yeast

Mutation

T406A, T407A, V416I

Available Since

March 21, 2012

Availability

Academic Institutions and Nonprofits only

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pAG304GPD-ccdB-HA

Plasmid

#14232

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

3HA

Expression

Yeast

Available Since

March 29, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
GAL 25Q+ProCFPp303

Plasmid

#15574

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt25Q + Pro (HTT Human)

Tags

CFP

Expression

Yeast

Mutation

Proline-rich region present

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pPS1890

Plasmid

#1362

Depositor

Pamela Silver

Article

Damelin et al Mol Cell 2000 Jan;5(1):133-40.

Insert

NLS-ECFP

Expression

Yeast

Available Since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
pRS413-IVa

Plasmid

#31458

Depositor

Ana Kitazono

Article

Kitazono et al Gene. 2011 Sep 15;484(1-2):86-9. doi: 10

Insert

linker

Expression

Yeast

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only
pAG416GAL-EYFP-ccdB

Plasmid

#14339

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

EYFP

Expression

Yeast

Available Since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pGADT7 Hsh155 WT

Plasmid

#87288

Purpose

Encodes Y2H Gal4 activation domain for Hsh155 WT

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Tags

GAL4 AD and HA

Expression

Bacterial and Yeast

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pEM01

Plasmid

#135414

Purpose

Expression of Hygromycin resistance from CMV promoter

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

CMVpr hph ADH1t

Expression

Bacterial and Yeast

Promoter

CMVpr

Available Since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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YEplac181-TAF14

Plasmid

#8625

Depositor

Jeff Strathern

Article

Malagon et al Genetics 2004 Mar;166(3):1215-27.

Insert

TAF14 (TAF14 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Wild type

Available Since

Nov. 15, 2005

Availability

Academic Institutions and Nonprofits only

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pRS406-GFP-SNC1(L96V)

Plasmid

#105278

Purpose

Integrating construct for expressing GFP-Snc1(L96V) from the TPI1 promoter.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Snc1 (SNC1 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

Leucine 96 changed to Valine, intron removed

Promoter

TPI1

Available Since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pPTK007-3a-αMFΔ

Plasmid

#84966

Purpose

Type 3a, α-mating factor pre-sequence shortened - secretion signal

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

α-mating factor, pre-sequence shortened secretion tag

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pJT45_GalL_nCDA1-BE3

Plasmid

#145038

Purpose

Expresses nCDA1-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

nCDA1-BE3

Use

CRISPR

Expression

Yeast

Mutation

spCas9(D10A)

Promoter

GalL

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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CUP Snf5 YFP p316

Plasmid

#15597

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

SNF5 (SNF5 Budding Yeast)

Tags

YFP

Expression

Yeast

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pK3FS-PYK1

Plasmid

#85777

Purpose

N-ICE plasmid pK3FS-PYK1

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-KanMX-loxP-PYK1p-3FLAG

Use

Cre/Lox

Expression

Yeast

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pAG414GPD-ccdB-DsRed

Plasmid

#14360

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

DsRed

Expression

Yeast

Available Since

March 29, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pJAID5VY

Plasmid

#165048

Purpose

Integrative expression of CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>CUP1-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3

Expression

Yeast

Mutation

OsTIR1 is codon-optimised for expression in S. ce…

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pPIC-mouseKir3.2-mC-S

Plasmid

#140745

Purpose

Expression of mouse Kir3.2 fused to mCherry and Streptag II

Depositor

Arthur Laganowsky

Article

Qiao et al Biochemistry. 2020 May 6. doi: 10.1021/a

Insert

Kir3.2 (Kcnj6 Mouse)

Tags

mCherry with Streptag II

Expression

Yeast

Promoter

AOX1

Available Since

May 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGBW-m4046380

Plasmid

#148977

Purpose

Yeast Expression plasmid for SARS-CoV-2 E (envelope)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2, Budding Yeast)

Tags

Cleavable thrombin;MBP

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pGAL1

Available Since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGBW-m4046710

Plasmid

#149178

Purpose

Yeast Expression plasmid for SARS-CoV-2 E (envelope)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2, Budding Yeast)

Expression

Yeast

Mutation

wt

Promoter

pTEF1

Available Since

May 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pPS1891

Plasmid

#1363

Depositor

Pamela Silver

Article

Damelin et al Mol Cell 2000 Jan;5(1):133-40.

Insert

NLS-EYFP

Expression

Yeast

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pFA6a-his3-P3nmt1-3FLAG

Plasmid

#19339

Depositor

Eishi Noguchi

Article

Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

Insert

his3 (his3 Fission Yeast)

Expression

Yeast

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG305GAL-ccdB-EYFP

Plasmid

#14209

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

EYFP

Expression

Yeast

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG416GPD-Cerulean-CHC1

Plasmid

#18850

Depositor

Susan Lindquist

Article

Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

Insert

CHC1 (CHC1 Budding Yeast)

Tags

Cerulean

Expression

Yeast

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pDA125

Plasmid

#74249

Purpose

Intermediate cloning vector, with MCS2 containing inducible unstable peptide driven by pSTL1 (UbiY 2xNLSs SZ1).

Depositor

Serge Pelet

Article

Aymoz et al Nat Commun. 2016 Apr 21;7:11304. doi: 10

Insert

Inducible peptide of the dPSTR

Expression

Bacterial and Yeast

Promoter

pSTL1

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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pRS416 ATG5-E141D-HA-ATG12-ATG16

Plasmid

#166851

Purpose

Expresses mutant E141D ATG5, HA-ATG12 ATG16 in yeast.

Depositor

Daniel Klionsky

Article

Kim et al Elife. 2016 Jan 26;5. pii: e12245. doi:

Insert

ATG5 ATG12 ATG16 (ATG5, ATG12, ATG16 Budding Yeast)

Tags

HA-ATG12

Expression

Yeast

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGBKT7-PRP9

Plasmid

#87593

Purpose

Encodes Y2H Gal4 DNA binding domain for Prp9

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

PRP9 (PRP9 Budding Yeast)

Tags

GAL4 BD and Myc

Expression

Bacterial and Yeast

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAV0835

Plasmid

#133512

Purpose

Expression of mCherry tagged CHD domain to monitor actin cytoskeleton

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pUra4(AfeI)-p(act1)-mCherry-CHDRng2-terminator(ScCYC1)

Expression

Yeast

Available Since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pWS932

Plasmid

#123055

Purpose

Pheromone responsive domain

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

PRD [3b]

Expression

Yeast

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pWS2119

Plasmid

#123048

Purpose

PbPeptide GPCR

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

PbSte2 [3]

Expression

Yeast

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pKT0212

Plasmid

#8725

Depositor

Kurt Thorn

Article

Sheff et al Yeast 2004 Jun;21(8):661-70.

Insert

yECFP A206R

Tags

Codon optimized linker

Expression

Yeast

Mutation

yEGFP with F64L, S65T, Y66W, N146I, M153T, V163A,…

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM0617

Plasmid

#128993

Purpose

loxP-KIURA3-loxP preassembled entry vector

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

KanR-ColE1 URA 5' homology-ConLS'-sfGFP dropout-ConRE'-loxP-KIURA3-loxP-URA3 5' homology

Expression

Yeast

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pICE LYS2-HIS3

Plasmid

#122887

Purpose

LYS2-HIS3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pICE LYS2-MET17

Plasmid

#122889

Purpose

LYS2-MET17 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pICE TRP1-LEU2

Plasmid

#122892

Purpose

TRP1-LEU2 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC119-Padh81-GBP-mCherry(N)-hphMX6-lys1*

Plasmid

#89072

Purpose

genomic GBP-mCherry epitope N-terminal tagging at lys1+ locus in S. pombe

Depositor

Quanwen Jin

Article

Chen et al J Cell Sci. 2017 Jan 12. pii: jcs.198457

Type

Empty backbone

Use

Genomic integration in s. pombe

Tags

GBP-mCherry

Expression

Yeast

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pICE ADE2-LEU2

Plasmid

#122884

Purpose

ADE2-LEU2 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCP5b

Plasmid

#37185

Depositor

Adam Bogdanove

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Type

Empty backbone

Expression

Yeast

Available Since

Dec. 6, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-TKanAX

Plasmid

#73896

Purpose

fission yeast drug counter-selectable selection marker

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Insert

TK-kan

Expression

Yeast

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
68Q-ymsfGFP

Plasmid

#128560

Purpose

Httex1 (without proline-rich region) fused to ymsfGFP

Depositor

Patrick Lajoie

Article

Albakri et al F1000Res. 2018 Aug 10;7:1242. doi: 10.12

Insert

68Q-Httex1 (without proline-rich region) fused to ymsfGFP

Tags

ymsfGFP

Expression

Yeast

Promoter

GAL1

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTH371-SUP45-N58A

Plasmid

#29378

Depositor

Tobias von der Haar

Article

Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

Insert

SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)

Expression

Yeast

Mutation

N58A mutant gene including genomic sequence 1432 …

Available Since

May 23, 2011

Availability

Academic Institutions and Nonprofits only
pTH397-SUP45-I222S

Plasmid

#29388

Depositor

Tobias von der Haar

Article

Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

Insert

SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)

Expression

Yeast

Mutation

I222S mutant gene (corresponding to the sup45-2 a…

Available Since

May 23, 2011

Availability

Academic Institutions and Nonprofits only
pBK1

Plasmid

#1213

Depositor

Susan Lindquist

Article

Nathan et al Mol Cell Biol 1995 Jul;15(7):3917-25.

Insert

Hsc82::LEU2 (HSC82 Budding Yeast)

Use

Integration

Expression

Yeast

Mutation

hsc82::LEU2 deletion select for plasmid using LE…

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pGBKT7-PRP43

Plasmid

#87596

Purpose

Encodes Y2H Gal4 DNA binding domain for Prp43

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

PRP43 (PRP43 Budding Yeast)

Tags

GAL4 BD and Myc

Expression

Bacterial and Yeast

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAD424-rSyntenin1

Plasmid

#89425

Purpose

Two-hybrid system

Depositor

Fritz Rathjen

Article

Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

Insert

SDCBP

Tags

Gal4 AD

Expression

Yeast

Promoter

ADH1

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
416-Met25 Kar2 printor HDEL

Plasmid

#32129

Depositor

Susan Lindquist

Article

Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

Insert

Printor (KLHL14 Human)

Tags

HDEL and Kar2

Expression

Yeast

Available Since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
p426 1-F GW

Plasmid

#58548

Purpose

pRS426 carrying Gateway cassette for a single insert

Depositor

Leonid Kruglyak

Type

Empty backbone

Expression

Yeast

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG306GAL-ccdB-TAP

Plasmid

#14259

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

TAP

Expression

Yeast

Available Since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pCHFLAGGR

Plasmid

#1279

Depositor

Susan Lindquist

Article

Kimura et al Science 1995 Jun 2;268(5215):1362-5.

Insert

GR (Nr3c1 Rat)

Tags

FLAG

Expression

Yeast

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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