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Plasmid#73618PurposeVisualizing the nuclear translocation of LINXa4-Bre1 in yeast.DepositorInsertVenus
UseSynthetic BiologyTagsFLAGExpressionYeastMutationBre1 point mutations K8A, K9A and K11APromoterADH1Available sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pRS406-PHO5-GFP-Scs2
Plasmid#58834PurposeExpresses GFP-Tagged Scs2 in yeastDepositorInsertScs2 (SCS2 Budding Yeast)
UseTagsGFP and MycExpressionYeastMutationPromoterPHO5Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
LD214
Plasmid#69185PurposeIntegration of 2GFP tag at Atg14 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg14-2EGFP
UseTags2xEGFPExpressionYeastMutationPromoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
LD205
Plasmid#69180PurposeIntegration of 2GFP tag at Atg5 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg5-2EGFP
UseTags2xEGFPExpressionYeastMutationPromoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAH235
Plasmid#121145PurposeCas9 expression controlled by the nmt41 promoterDepositorInserthumanized Streptococcus pyogenes Cas9
UseCRISPRTagsFlagExpressionYeastMutationPromoternmt41Available sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
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D1433 his3::LYS2 Disruptor Converter
Plasmid#51676PurposeYeast marker swap plasmid for converting HIS3 to LYS2DepositorInsertLYS2 (LYS2 Budding Yeast)
UseYeast marker swapTagsExpressionYeastMutationPromoterAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTH371-SUP45-N58A
Plasmid#29378DepositorInsertSUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
UseTagsExpressionYeastMutationN58A mutant gene including genomic sequence 1432 …PromoterAvailable sinceMay 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTH397-SUP45-I222S
Plasmid#29388DepositorInsertSUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
UseTagsExpressionYeastMutationI222S mutant gene (corresponding to the sup45-2 a…PromoterAvailable sinceMay 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAG304GPD-ccdB-HA
Plasmid#14232DepositorTypeEmpty backboneUseGateway destinationTags3HAExpressionYeastMutationPromoterAvailable sinceMarch 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRS316-RGR-GFP-mHDV
Plasmid#51058PurposeSame as pRS316-RGR-GFP except for the HDV ribozyme region which has a 15 bp deletion at its 3' end.DepositorInsertRGR-GFP
UseCRISPRTagsExpressionBacterial and YeastMutationPromoterYeast ADH1 promoterAvailable sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRS316-RGR-GFP-mHH
Plasmid#51057PurposeSame as pRS316-RGR-GFP except for the HH ribozyme region which has a 13 bp deletion at its 5' end.DepositorInsertRGR-GFP
UseCRISPRTagsExpressionBacterial and YeastMutationPromoterYeast ADH1 promoterAvailable sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGADT7 Hsh155 WT
Plasmid#87288PurposeEncodes Y2H Gal4 activation domain for Hsh155 WTDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsGAL4 AD and HAExpressionBacterial and YeastMutationPromoterAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIRES:mTREK-1(K271Q)
Plasmid#133273PurposeMammalian expression vector. It will generate the mouse TREK-1 channel full lenght K271Q mutant fused to a N-terminal HA tagDepositorInsertPotassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)
UseTagsHAExpressionMammalian and YeastMutationK271QPromoterAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS316-IVa
Plasmid#31456DepositorInsertlinker
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHES262 [prADH1-Msn2-mCherry, pnh604]
Plasmid#103844PurposeYeast Expression PlasmidDepositorInsertpAdh1-Msn2-mCherry
UseTagsmCherryExpressionYeastMutationPromoterAvailable sinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMV13
Plasmid#128864PurposePosition 1 module- CEN (Centromeric)DepositorInsertCEN/ARS
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
LHP308 - Ub - wt
Plasmid#32151DepositorUseTagsExpressionYeastMutationPromoterCUP1Available sinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJAS1669
Plasmid#158594PurposeVioE-dead_ePTS1 CEN6/ARS4DepositorInsertVioE-dead_ePTS1
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG414GAL-ccdB-TAP
Plasmid#14263DepositorTypeEmpty backboneUseGateway destinationTagsTAPExpressionYeastMutationPromoterAvailable sinceMarch 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
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pFA3
Plasmid#46790PurposeTo express ATPase inactive Fun30 in budding yeastDepositorInsertFUN30 (FUN30 Budding Yeast)
UseTagsV5ExpressionYeastMutationATP-binding site mutated replaced lysin 603 by ar…PromoterPGAL1Available sinceAug. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRS406-GFP-SNC1(L96V)
Plasmid#105278PurposeIntegrating construct for expressing GFP-Snc1(L96V) from the TPI1 promoter.DepositorInsertSnc1 (SNC1 Budding Yeast)
UseTagsGFPExpressionYeastMutationLeucine 96 changed to Valine, intron removedPromoterTPI1Available sinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPTK007-3a-αMFΔ
Plasmid#84966PurposeType 3a, α-mating factor pre-sequence shortened - secretion signalDepositorInsertα-mating factor, pre-sequence shortened secretion tag
UseSynthetic BiologyTagsExpressionYeastMutationPromoterNonAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS414-GPD-6xHis-Hsp82
Plasmid#41188DepositorInsertS. cerevisiae Hsp82
UseTags6xHisExpressionYeastMutationPromoterGPDAvailable sinceNov. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
LD213
Plasmid#69184PurposeIntegration of 2GFP tag at Atg13 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg13-2EGFP
UseTags2xEGFPExpressionYeastMutationPromoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS414-7x2-PHO5-GFP-hPLC delta PH domain dimer
Plasmid#58837PurposeExpresses GFP-Tagged Human PLC delta PH domain in yeastDepositorInsert2x Phospolipase C delta1 PH domain (Plcd1 Rat)
UseTagsGFPExpressionYeastMutationPromoterPHO5Available sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pK3FS-PYK1
Plasmid#85777PurposeN-ICE plasmid pK3FS-PYK1DepositorInsertloxP-KanMX-loxP-PYK1p-3FLAG
UseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
YIp TRP1-URA3
Plasmid#122915PurposeTRP1-URA3 integrating (YIp) vector for stable genomic integration in the BY4741 background.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAG424GAL-ccdB-EYFP
Plasmid#14223DepositorTypeEmpty backboneUseGateway destinationTagsEYFPExpressionYeastMutationPromoterAvailable sinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pILGFPQ3
Plasmid#83558PurposeUsed to evaluate the expression output of GAL2 promoter with yEGFP used as the reporter gene.DepositorInsertGAL2 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterGAL2Available sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGBKT7-PRP9
Plasmid#87593PurposeEncodes Y2H Gal4 DNA binding domain for Prp9DepositorInsertPRP9 (PRP9 Budding Yeast)
UseTagsGAL4 BD and MycExpressionBacterial and YeastMutationPromoterAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYC64
Plasmid#63928PurposeRecipient replicative vector to construct FLAG tagged fusionsDepositorTypeEmpty backboneUseTagsFLAGExpressionYeastMutationPromoterAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYC62
Plasmid#63927PurposeRecipient replicative vector to construct HA tagged fusionsDepositorTypeEmpty backboneUseTagsHAExpressionYeastMutationPromoterAvailable sinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAG424GAL-EYFP-ccdB
Plasmid#14343DepositorTypeEmpty backboneUseGateway destinationTagsEYFPExpressionYeastMutationPromoterAvailable sinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
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pDA125
Plasmid#74249PurposeIntermediate cloning vector, with MCS2 containing inducible unstable peptide driven by pSTL1 (UbiY 2xNLSs SZ1).DepositorInsertInducible peptide of the dPSTR
UseTagsExpressionBacterial and YeastMutationPromoterpSTL1Available sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046534
Plasmid#145571PurposeYeast Expression plasmid for SARS-CoV-2 ORF10 proteinDepositorInsertSARS-CoV-2 ORF10 protein (ORF10 Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;MBPExpressionYeastMutationwtPromoterpGAL1Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
YIp204-P30-His-POL30
Plasmid#99550PurposeYeast integrative vector for expression of His-tagged POL30 (PCNA); TRP1 markerDepositorInsertPOL30 (POL30 Budding Yeast)
UseTags6xHisExpressionYeastMutationPromoterPOL30Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only