8,033 results

1. pAG416GPD-SEC7-Cerulean

   Plasmid

   #18851

   Depositor

   Susan Lindquist

   Article

   Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

   Insert

   SEC7 (SEC7 Budding Yeast)
   

   Use

   Tags

   Cerulean

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 21, 2008

   Availability

   Academic Institutions and Nonprofits only

2. pKT0232

   Plasmid

   #8727

   Depositor

   Kurt Thorn

   Article

   Sheff et al Yeast 2004 Jun;21(8):661-70.

   Insert

   yECFP
   

   Use

   Tags

   13x Myc and Codon optimized linker

   Expression

   Yeast

   Mutation

   yEGFP with F64L, S65T, Y66W, N146I, M153T, V163A

   Promoter

   Available since

   Feb. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCfB4929

   Plasmid

   #126751

   Purpose

   Expresses pTRX2_5xUAS-ALD6-tADH1 in yeast from plasdmid pRS413

   Depositor

   Michael Jensen

   Article

   Zhang et al ACS Synth Biol. 2016 Dec 16;5(12):1546-1

   Insert

   ALD6
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pTRX2_5xUAS

   Available since

   June 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pNB655

   Plasmid

   #60142

   Purpose

   Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by MET17 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   FLuc-yEVenus
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   A4V

   Promoter

   MET17pr

   Available since

   Feb. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCfB2800

   Plasmid

   #63657

   Purpose

   pCfB2800 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

   Depositor

   Irina Borodina, Jerome Maury

   Article

   Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

   Insert

   GFP
   

   Use

   Cre/Lox

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1 promoter

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. MCA2-eGFP

   Plasmid

   #117098

   Purpose

   protein expression in yeast

   Depositor

   Geoffrey Chang

   Insert

   MCA2-eGFP
   

   Use

   Tags

   His

   Expression

   Yeast

   Mutation

   Promoter

   AOX

   Available since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. MCA1-eGFP

   Plasmid

   #117097

   Purpose

   protein expression in yeast

   Depositor

   Geoffrey Chang

   Insert

   MCA1-eGFP
   

   Use

   Tags

   His

   Expression

   Yeast

   Mutation

   Promoter

   AOX

   Available since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDTN3997

   Plasmid

   #113337

   Purpose

   Multi-copy 2-pyrone synthase plasmid for triacetic acid lactone production in Yarrowia lipolytica

   Depositor

   Suzanne Sandmeyer

   Article

   Yu et al Biotechnol Bioeng. 2018 May 19. doi: 10.

   Insert

   multi-copy 2-pyrone synthase
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

9. pCfB3049(gRNA XII-4)

   Plasmid

   #73291

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-4

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   guiding RNA
   

   Use

   CRISPR; Grna

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUDP123

    Plasmid

    #107269

    Purpose

    pUDP123 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes OpADE2 and OpNIAD and Spcas9D147Y P411T in O. parapolymorpha (HH-gRNAOpADE2-HDV-linker-HH-gRNAOpNIAD-HDV)

    Depositor

    Jean-Marc Daran

    Article

    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

    Insert

    polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting OpADE2 and NIAD in O. parapolymorpha
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScTDH3

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pUR-sap1+

    Plasmid

    #15984

    Depositor

    Eishi Noguchi

    Article

    Noguchi et al Genetics. 2007 Feb . 175(2):553-66.

    Insert

    Sap1 (sap1 Fission Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 31, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pAS1

    Plasmid

    #12388

    Depositor

    Nick Rhind

    Article

    Sigova et al Genes Dev. 2004 Oct 1. 18(19):2359-67.

    Insert

    green fluorescent protein
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCfB8382

    Plasmid

    #126916

    Purpose

    Plasmid containing integration cassette at XI-3 integration site in Saccharomyces cerevisiae for overexpression of XdCrtI

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    XdCrtI
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHES553 [prGal1-RAS2(KAN) pnh604]

    Plasmid

    #103846

    Purpose

    Yeast Expression Plasmid

    Depositor

    Hana El-Samad

    Article

    Stewart-Ornstein et al Mol Biol Cell. 2017 Jan 1;28(1):221-227.

    Insert

    pGal1-Ras2(KAN)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. YCplac111-yVps23-mCherry

    Plasmid

    #45925

    Depositor

    James Hurley

    Article

    Ren et al EMBO J. 2011 Jun 1;30(11):2130-9. doi: 1

    Insert

    yeast Vps23 (STP22 Budding Yeast)
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    lac

    Available since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pRS316Nab3

    Plasmid

    #110533

    Purpose

    Expresses yeast NAB3 using the endogenous promoter

    Depositor

    Daniel Reines

    Article

    Loya et al Nucleic Acids Res. 2012 Aug;40(15):7476-

    Insert

    NAB3 (NAB3 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Ube2m

    Plasmid

    #31423

    Depositor

    Rachel Klevit

    Article

    Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

    Insert

    Ubiquitin Conjugating Enzyme (UBE2M Human)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    N/A

    Promoter

    Available since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pG1-NMGR526

    Plasmid

    #1122

    Depositor

    Susan Lindquist

    Article

    Li et al Science 2000 Jan 28;287(5453):661-4.

    Insert

    sup35 NM domain fused with GR (SUP35, Nr3c1 Rat, Budding Yeast)
    

    Use

    Tags

    GR526

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 3, 2005

    Availability

    Academic Institutions and Nonprofits only

19. pDRF1-GW tdTomato-T2A-mTurquoise2

    Plasmid

    #118439

    Purpose

    Produces equimolar expression of tdTomato and mTq2

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    tdTomato-T2A-mTurquoise2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 413-Met25 Kar2 TorsinA E171Q GFP

    Plasmid

    #32127

    Depositor

    Susan Lindquist

    Article

    Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

    Insert

    TorsinA (TOR1A Human)
    

    Use

    Tags

    GFP and Kar2

    Expression

    Yeast

    Mutation

    E171Q

    Promoter

    Available since

    Sept. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

21. pYes2-AtPCS1

    Plasmid

    #49766

    Purpose

    Expresses Arabidopsis phytochelatin synthase (AtPCS1) in Saccharomyces Cerevisiae

    Depositor

    Julian Schroeder

    Article

    Clemens et al EMBO J. 1999 Jun 15;18(12):3325-33.

    Insert

    AtPCS1 (PCS1 Mustard Weed)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Jan. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

22. pEM01

    Plasmid

    #135414

    Purpose

    Expression of Hygromycin resistance from CMV promoter

    Depositor

    Nicolas Buchler

    Article

    Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

    Insert

    CMVpr hph ADH1t
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    CMVpr

    Available since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. YIp ADE2-MET17

    Plasmid

    #122906

    Purpose

    ADE2-MET17 integrating (YIp) vector for stable genomic integration in the BY4741 background.

    Depositor

    Randy Hampton

    Article

    Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

    Type

    Empty backbone

    Use

    Yeast recombination cloning

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJT45_GalL_nCDA1-BE3

    Plasmid

    #145038

    Purpose

    Expresses nCDA1-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

    Insert

    nCDA1-BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A)

    Promoter

    GalL

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJAID5VY

    Plasmid

    #165048

    Purpose

    Integrative expression of CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.

    Depositor

    Claudia Vickers

    Article

    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

    Insert

    P-URA3>KlURA3>T-AgTEF1-P-TEF1>CUP1-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    OsTIR1 is codon-optimised for expression in S. ce…

    Promoter

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pPIC-mouseKir3.2-mC-S

    Plasmid

    #140745

    Purpose

    Expression of mouse Kir3.2 fused to mCherry and Streptag II

    Depositor

    Arthur Laganowsky

    Article

    Qiao et al Biochemistry. 2020 May 6. doi: 10.1021/a

    Insert

    Kir3.2 (Kcnj6 Mouse)
    

    Use

    Tags

    mCherry with Streptag II

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    May 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. p2HG/N795

    Plasmid

    #1284

    Depositor

    Susan Lindquist

    Article

    Picard et al Nature 1990 Nov 8;348(6297):166-8.

    Insert

    GR (Nr3c1 Rat)
    

    Use

    Tags

    GPD promoter

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

28. pAG306GAL-EGFP-ccdB

    Plasmid

    #14307

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAG425GPD-ccdB-ECFP

    Plasmid

    #14178

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    ECFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGBW-m4046380

    Plasmid

    #148977

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;MBP

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. pGBW-m4046710

    Plasmid

    #149178

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available since

    May 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pPS2045

    Plasmid

    #1376

    Depositor

    Pamela Silver

    Article

    Brodsky et al RNA 2000 Dec;6(12):1737-49.

    Insert

    U1A (1-94) (RNU1-4 Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    U1A (1-94)

    Promoter

    Available since

    Aug. 3, 2005

    Availability

    Academic Institutions and Nonprofits only

33. pREP4xN_ccdB

    Plasmid

    #79022

    Purpose

    Gateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, ura4+ selection

    Depositor

    Pernilla Bjerling, Göran Akusjärvi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pRS416 ATG5-E141D-HA-ATG12-ATG16

    Plasmid

    #166851

    Purpose

    Expresses mutant E141D ATG5, HA-ATG12 ATG16 in yeast.

    Depositor

    Daniel Klionsky

    Article

    Kim et al Elife. 2016 Jan 26;5. pii: e12245. doi:

    Insert

    ATG5 ATG12 ATG16 (ATG5, ATG12, ATG16 Budding Yeast)
    

    Use

    Tags

    HA-ATG12

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAV0835

    Plasmid

    #133512

    Purpose

    Expression of mCherry tagged CHD domain to monitor actin cytoskeleton

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pUra4(AfeI)-p(act1)-mCherry-CHDRng2-terminator(ScCYC1)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pWS932

    Plasmid

    #123055

    Purpose

    Pheromone responsive domain

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    PRD [3b]
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pWS2119

    Plasmid

    #123048

    Purpose

    PbPeptide GPCR

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    PbSte2 [3]
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMM0617

    Plasmid

    #128993

    Purpose

    loxP-KIURA3-loxP preassembled entry vector

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    KanR-ColE1 URA 5' homology-ConLS'-sfGFP dropout-ConRE'-loxP-KIURA3-loxP-URA3 5' homology
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pPTK015-3-yEGFP_Intra

    Plasmid

    #84974

    Purpose

    Type 3, Green fluorescent protein

    Depositor

    Volker Sieber

    Article

    Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

    Insert

    Green fluorescent protein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Non

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMZ381

    Plasmid

    #59899

    Purpose

    Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6-L469

    Depositor

    Mikel Zaratiegui

    Article

    Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

    Inserts

    Cas9
    rrk1:sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Silent muation of the CspCI site

    Promoter

    Available since

    Oct. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge