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We narrowed to 2,572 results for: GEM

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Showing: 1 - 40 of 2572 results

  2. GEM

    Plasmid
    #38820
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GEM (Gem Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  3. hSyn-GEM

    Plasmid
    #187648
    Purpose
    Expresses the rheological probe PfV-HaloTag-SNAP-tag (GEM-HaloTag-SNAP-tag) under control of human synapsin promoter.
    Depositor
    Erin O'Shea
    Article
    Wong et al Elife. 2024 Aug 15;13:e80622. doi: 10.75
    Insert
    PfV
    Use
    AAV
    Tags
    HaloTag and SNAP-tag
    Expression
    Mammalian
    Promoter
    Synapsin
    Available Since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    187648-plasmid-map-sequence-id-369068

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  10. pGEM-2t

    Plasmid
    #159752
    Purpose
    CRISPR/Cas9 genome editing in plants; a template for PCR-derived fragments used in the assembly of polycistronic transcripts, where sgRNAs are separated by an Arabidopsis alanine tRNA.
    Depositor
    Wolfgang Lukowitz
    Article
    Angulo et al bioRxiv
    Insert
    sgRNA
    Use
    CRISPR
    Available Since
    Oct. 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    159752-plasmid-map-sequence-id-313824

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  16. mOtop1_pGEMHE

    Plasmid
    #114674
    Purpose
    This construct was used to express mouse Otop1 in Xenopus laevis oocytes
    Depositor
    Emily Liman
    Article
    Tu et al Science. 2018 Mar 2;359(6379):1047-1050.
    Insert
    Otopetrin 1 (Otop1 Mouse)
    Use
    For generating the mrna, which can highly express…
    Mutation
    SNP (rs49325632): Glycine 396 to Alanine
    Promoter
    T7
    Available Since
    July 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    114674-plasmid-map-sequence-id-226716

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  18. pGEM_ΔndhD2.km

    Plasmid
    #185532
    Purpose
    a pGEM-T easy vector containing the kanamycin resistance cassette flanked by the two regions for the double homologous recombination on the ndhD2 locus
    Depositor
    Paula Tamagnini
    Article
    Spasic et al J Biotechnol. 2022 Dec 10;360:152-159. d
    Insert
    nptII gene flanked by ndhD2 upstream and downstream regions
    Use
    Synthetic Biology
    Available Since
    Oct. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    185532-plasmid-map-sequence-id-368416

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  19. pGEM_Δflv3.sm

    Plasmid
    #185533
    Purpose
    a pGEM-T easy vector containing the streptomycin/spectinomycin resistance cassette flanked by the two regions for the double homologous recombination on the flv3 locus
    Depositor
    Paula Tamagnini
    Article
    Spasic et al J Biotechnol. 2022 Dec 10;360:152-159. d
    Insert
    aadA gene flanked by flv3 upstream and downstream regions
    Use
    Synthetic Biology
    Available Since
    Oct. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    185533-plasmid-map-sequence-id-371409

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  30. eSpCas9-hGeminin

    Plasmid
    #199344
    Purpose
    modified version of the eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA plasmid (addgene #86613) where the C-terminus of the eSpCas9 enzyme was fused to amino acids 1 – 110 of human Geminin
    Depositor
    Iain Hagan
    Article
    Morrison et al Open Biol. 2023 Apr;13(4):230021. doi: 1
    Insert
    ATP1A1 G3 sgRNA+user-specified sgRNA+enhanced specificity Cas9 (1.1) (addgene 86613) with cas9 fused to hgem fragment 1-110) (GMNN S. pyogenes)
    Tags
    cas9 c-term fused to hgemenin 1-110 fragment
    Expression
    Mammalian
    Mutation
    cas9 c-term fused to hgemenin 1-110 fragment
    Promoter
    cbh
    Available Since
    Dec. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    199344-plasmid-map-sequence-id-402934

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  31. pGEMS-II-PEST

    Plasmid
    #204766
    Purpose
    GEMS 2.0 sensor system with PEST destabilization domain (pCMV-EGFP-PEST-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577+PEST)
    Depositor
    Kate Meyer
    Article
    Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038
    Insert
    EGFP-PEST-m6Alinker-DHFR
    Mutation
    mutated all RACs in EGFP to RGC/RCC
    Promoter
    CMV
    Available Since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    204766-plasmid-map-sequence-id-408653

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  32. pGEM5Z(+)-EGFP

    Plasmid
    #65206
    Purpose
    EGFP expression plasmid (also called p111) for the creation of a heteroduplex DNA repair assay plasmid with p189. *Please see notes in comments section.
    Depositor
    LuZhe Sun
    Article
    Zhou et al Anal Biochem. 2009 May 1;388(1):167-9. d
    Insert
    EGFP
    Expression
    Bacterial and Mammalian
    Mutation
    Restriction enzyme recognition sites added either…
    Promoter
    CMV
    Available Since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    65206-plasmid-map-sequence-id-490956

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Showing: 1 - 40 of 2572 results