We narrowed to 383 results for: Pef1

1. PROM5_MpEF1a

   Plasmid

   #136113

   Purpose

   Strong promoter from MpEF1a, that drives expression everywhere in Mp gemmae but stronger in the notches

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   PROM5_MpEF1a Mapoly0024s0116
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pEF1a-hMLH1dn

   Plasmid

   #174824

   Purpose

   Mammalian expression of human MLH1dn (codon optimized)

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   human MLH1dn (codon optimized)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   deletion of residues 754-756

   Promoter

   EF1a

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEF1-alpha-V

   Plasmid

   #27290

   Depositor

   Timothy Springer

   Article

   Takagi et al Cell. 2002 Sep 6. 110(5):599-11.

   Insert

   integrin alpha V (ITGAV Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Isoform 2

   Promoter

   Available since

   March 8, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEF1α:flex-mcherry

   Plasmid

   #222618

   Purpose

   Cre-dependent expression of the red fluorescent protein mCherry under control of the EF1a promoter

   Depositor

   Franck Polleux

   Article

   Blockus et al Cell Rep. 2021 Oct 19;37(3):109828. doi:

   Insert

   Cre-dependent mCherry
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEF1a-mMLH1dn

   Plasmid

   #174825

   Purpose

   Mammalian expression of mouse MLH1dn (codon optimized)

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   mouse MLH1dn (codon optimized)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   deletion of residues 758-760

   Promoter

   EF1a

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDV05.0_pEF1a-iRFP720

   Plasmid

   #200407

   Purpose

   Expresses iRFP720 constitutively in mammalian cells

   Depositor

   James Collins

   Article

   Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

   Insert

   iRFP720
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDV05.2_pEF1a-iRFP720_secreted

   Plasmid

   #200409

   Purpose

   Expresses a secreted version of iRFP720 constitutively in mammalian cells

   Depositor

   James Collins

   Article

   Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

   Insert

   iRFP720 (secreted)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEF1a-mRor2WT

   Plasmid

   #22613

   Depositor

   Roel Nusse

   Article

   Mikels et al J Biol Chem. 2009 Aug 31. ():.

   Insert

   Ror2 WT (Ror2 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 24, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEF1-His-Ub

   Plasmid

   #159326

   Purpose

   Mammalian cell expression of mouse His-Ub

   Depositor

   Jianjun Zhao

   Insert

   His-Ub
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 33-G-X5-pEF1/V5-His

    Plasmid

    #111605

    Purpose

    Express human 33-G-X5 in 293T cells

    Depositor

    Timothy Springer

    Article

    Qin et al Cell. 2018 Jun 28;174(1):156-171.e16. do

    Insert

    human LRRC33 and GARP chimeria (NRROS Human)
    

    Use

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEF1a_CD WT TET1

    Plasmid

    #124082

    Purpose

    Expression of wild-type catalytic domain only form of TET1

    Depositor

    Silvia Monticelli

    Article

    Vincenzetti et al Eur J Immunol. 2019 Jan 30. doi: 10.1002

    Insert

    TET1 catalytic domain (TET1 Human)
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1 alpha

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEF1a-mRor2N620K

    Plasmid

    #22631

    Depositor

    Roel Nusse

    Article

    Mikels et al J Biol Chem. 2009 Aug 31. ():.

    Insert

    Ror2 N620K (Ror2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Changed Asparagine 620 to Lysine

    Promoter

    Available since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

13. pDV05.1_pEF1a-iRFP720_frameshifted

    Plasmid

    #200408

    Purpose

    Expresses a mutant iRFP720 mRNA constitutively in mammalian cells. A frameshift deletion in the ORF (compared to pDV05.0) prevents the correct translation of the fluorescent protein

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    iRFP720 (frameshift mutant)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEF1a-nSpCas9-NG

    Plasmid

    #171692

    Purpose

    Expresses nSpCas9-NG in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

    Insert

    nSpCas9-NG
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1…

    Promoter

    EF1a

    Available since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEF1a-nSpCas9

    Plasmid

    #171691

    Purpose

    Expresses nSpCas9 in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

    Insert

    nSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    EF1a

    Available since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEF1a-mRor2deltaIg

    Plasmid

    #22629

    Depositor

    Roel Nusse

    Article

    Mikels et al J Biol Chem. 2009 Aug 31. ():.

    Insert

    Ror2 delta Ig (Ror2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Deletion of Ig domain

    Promoter

    Available since

    Nov. 25, 2009

    Availability

    Academic Institutions and Nonprofits only

17. pEF1a-mRor2delta745

    Plasmid

    #22640

    Depositor

    Roel Nusse

    Article

    Mikels et al J Biol Chem. 2009 Aug 31. ():.

    Insert

    Ror2 delta 745 (Ror2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Deletion C terminal domain

    Promoter

    Available since

    Dec. 18, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pEF1a-mRor2deltaKringle

    Plasmid

    #22630

    Depositor

    Roel Nusse

    Article

    Mikels et al J Biol Chem. 2009 Aug 31. ():.

    Insert

    Ror2 delta Kringle (Ror2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Deletion of Kringle domain

    Promoter

    Available since

    Nov. 25, 2009

    Availability

    Academic Institutions and Nonprofits only

19. pEF1a-mRor2deltaPro

    Plasmid

    #22634

    Depositor

    Roel Nusse

    Article

    Mikels et al J Biol Chem. 2009 Aug 31. ():.

    Insert

    Ror2 delta Pro (Ror2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Deletion of Pro domain

    Promoter

    Available since

    Dec. 18, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pEF1a-T2-CeNLuc

    Plasmid

    #135948

    Purpose

    Expresses the CeNLuc LumiFluor targeted to the mitochondira in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    CeNLuc LumiFluor
    

    Use

    Tags

    T2 mitochondrial localization sequence

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pEF1a-mRor2Y641,645,646F

    Plasmid

    #22638

    Depositor

    Roel Nusse

    Article

    Mikels et al J Biol Chem. 2009 Aug 31. ():.

    Insert

    Ror2 Y641,645,646F (Ror2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Changed Tyrosine 641, 645 and 646 to Phenylalanine

    Promoter

    Available since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

22. 33-G-X1-pEF1-puro

    Plasmid

    #111604

    Purpose

    Express human 33-G-X1 in 293T cells

    Depositor

    Timothy Springer

    Article

    Qin et al Cell. 2018 Jun 28;174(1):156-171.e16. do

    Insert

    human LRRC33 and GARP chimeria (NRROS Human)
    

    Use

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEF1a-T2-NLuc

    Plasmid

    #135938

    Purpose

    Expresses NanoLuciferase targeted to the mitochondira in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    NanoLuciferase
    

    Use

    Tags

    T2 mitochondrial localization sequence

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEF1a-mRor2deltaST1

    Plasmid

    #22632

    Depositor

    Roel Nusse

    Article

    Mikels et al J Biol Chem. 2009 Aug 31. ():.

    Insert

    Ror2 delta ST1 (Ror2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Deletion of ST1 domain

    Promoter

    Available since

    Nov. 25, 2009

    Availability

    Academic Institutions and Nonprofits only

25. pEF1a-mRor2deltaST2

    Plasmid

    #22633

    Depositor

    Roel Nusse

    Article

    Mikels et al J Biol Chem. 2009 Aug 31. ():.

    Insert

    Ror2 delta ST2 (Ror2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Deletion of ST2 domain

    Promoter

    Available since

    Nov. 25, 2009

    Availability

    Academic Institutions and Nonprofits only

26. pEF1a-Foxd3-ER

    Plasmid

    #37270

    Depositor

    Patricia Labosky

    Insert

    Foxd3 (Foxd3 Mouse)
    

    Use

    Tags

    ER

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    Aug. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

27. pEF1Cas-LT1

    Plasmid

    #210556

    Purpose

    Binary vector expressing the Lineage Tracing system. Cas9 expression driven by M. polymorpha EF1 promoter. LT1 reporter under control of MpEF1p

    Depositor

    Ortrun Mittelsten Scheid

    Article

    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

    Insert

    pMpEF1::Cas9::PEA3AT::U6p::AAVS1gRNA::EF1p::LT1::Hygromycin
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEF1a-T2-GpNLuc

    Plasmid

    #135949

    Purpose

    Expresses the GpNLuc LumiFluor targeted to the mitochondira in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    GpNLuc LumiFluor
    

    Use

    Tags

    T2 mitochondrial localization sequence

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEF1a-T2-OgNLuc

    Plasmid

    #135951

    Purpose

    Expresses the OgNLuc LumiFluor targeted to the mitochondira in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    OgNLuc LumiFluor
    

    Use

    Tags

    T2 mitochondrial localization sequence

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEF1a-mRor2Y641F

    Plasmid

    #22635

    Depositor

    Roel Nusse

    Article

    Mikels et al J Biol Chem. 2009 Aug 31. ():.

    Insert

    Ror2 Y641F (Ror2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Changed Tyrosine 641 to Phenylalanine

    Promoter

    Available since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

31. pEF1a-mRor2Y645F

    Plasmid

    #22636

    Depositor

    Roel Nusse

    Article

    Mikels et al J Biol Chem. 2009 Aug 31. ():.

    Insert

    Ror2 Y645F (Ror2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Changed Tyrosine 645 to Phenylalanine

    Promoter

    Available since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

32. pEF1a-mRor2Y646F

    Plasmid

    #22637

    Depositor

    Roel Nusse

    Article

    Mikels et al J Biol Chem. 2009 Aug 31. ():.

    Insert

    Ror2 Y646F (Ror2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Changed Tyrosine 646 to Phenylalanine

    Promoter

    Available since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

33. pMV1 pEF1a-HGF

    Plasmid

    #188748

    Purpose

    Vector constitutively expressing HGF

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    HGF (HGF Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pEF1α-Cre-2A-Puro

    Plasmid

    #207743

    Purpose

    Expression of the Cre recombinase linked to a puro selectable marker

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    Cre-2A-Puro
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. pEF1α-Cre-2A-mScarlet

    Plasmid

    #207745

    Purpose

    Expression of the Cre recombinase linked to a mScarlet fluorescent marker

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    Cre-PGK-mScarlet
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. pEF1a-IRES-Neo

    Plasmid

    #28019

    Depositor

    Thomas Zwaka

    Article

    Dejosez et al Genes Dev. 2010 Jul 15. 24(14):1479-84.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAAV-pEF1a-tTAc

    Plasmid

    #172123

    Purpose

    Constitutive expression of InteinC-tTAC

    Depositor

    Chun Xu

    Article

    Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

    Insert

    InteinC-tTAc
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pEF1α-Cre-2A-mTagBFP2

    Plasmid

    #207746

    Purpose

    Expression of the Cre recombinase linked to a mTagBFP2 fluorescent marker

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    Cre-PGK-mTagBFP2
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

39. pEF1a-BirA-V5-neo

    Plasmid

    #100548

    Purpose

    Expresses C-terminal V5-tagged BirA biotin ligase

    Depositor

    Jian Xu

    Article

    Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

    Insert

    C-terminal V5-tagged BirA
    

    Use

    Tags

    V5, His

    Expression

    Mammalian

    Mutation

    Promoter

    Human EF1a

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLacO-pEF1α-RFP

    Plasmid

    #179507

    Purpose

    Expresses monomeric RFP in mammalian cells under a EF1α promoter

    Depositor

    Yasushi Hiraoka

    Article

    Haraguchi et al Commun Biol. 2022 Jan 20;5(1):78. doi: 1

    Insert

    monomeric RFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge