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Showing: 1 - 40 of 234 results

  3. AAVS1_Puro_PGK1_3xFLAG_Twin_Strep

    Plasmid
    #68375
    Purpose
    Doyon lab Tandem-Affinity Purification Following (i) Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus and (ii) Endogenous TAP Tagging.
    Depositor
    Yannick Doyon
    Article
    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and TALEN; Zfn
    Tags
    3xFLAG_Twin_Strep
    Expression
    Mammalian
    Mutation
    Promoter
    human PGK1
    Available since
    Oct. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    68375-plasmid-map-sequence-id-251912

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  13. ATP1A1_T804N_hPGK1_mScarlet-I-TOSI_Donor

    Plasmid
    #173208
    Purpose
    HDR donor plasmid to introduce the T804N mutation conferring cellular resistance to ouabain and the mScarlet-I mTOR signaling indicator (TOSI) cassette to ATP1A1 intron 17.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1-T804N mScarlet-I-TOSI donor
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hPGK1
    Available since
    April 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    173208-plasmid-map-sequence-id-339517

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  16. hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(1-100)Cy-

    Plasmid
    #191390
    Purpose
    Donor for targeted integration of hCdt1(1-100)Cy- probe to the hROSA26 safe harbor locus
    Depositor
    Yannick Doyon
    Insert
    hCdt1(1-100)Cy-
    Use
    Tags
    mScarlet-I
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    191390-plasmid-map-sequence-id-378098

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  17. hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(30-120)

    Plasmid
    #191391
    Purpose
    Donor for targeted integration of hCdt1(30-120) probe to the hROSA26 safe harbor locus
    Depositor
    Yannick Doyon
    Insert
    hCdt1(30-120)
    Use
    CRISPR and Synthetic Biology
    Tags
    mScarlet-I
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    191391-plasmid-map-sequence-id-378101

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  18. hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(1-100)

    Plasmid
    #191389
    Purpose
    Donor for targeted integration of hCdt1(1-100) probe to the hROSA26 safe harbor locus
    Depositor
    Yannick Doyon
    Insert
    hCdt1(1-100)
    Use
    Tags
    mScarlet-I
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    191389-plasmid-map-sequence-id-378099

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  25. pGL4.22-PGK1-HRE::dLUC

    Plasmid
    #128095
    Purpose
    Hypoxia response element real-time luciferase reporter
    Depositor
    Chi Van Dang
    Article
    Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:
    Insert
    Hypoxia response element (x3) from PGK1 promoter (PGK1 Human)
    Use
    Luciferase
    Tags
    drives expression of destabilized luciferase (2CP…
    Expression
    Mammalian
    Mutation
    Promoter
    inserted PGK1 hypoxia response elements (HRE) (x3)
    Available since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    128095-plasmid-map-sequence-id-249667

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  26. AAVS1_Puro_hPGK1_hGEM(1-110)_mNG_hSLBP(18-108)

    Plasmid
    #176932
    Purpose
    Donor for targeted integration of S-phase specific probe (FUCCI-S) to the AAVS1 safe harbor locus
    Depositor
    Yannick Doyon
    Insert
    hGEM(1-110)_mNG_hSLBP(18-108)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PGK1
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    176932-plasmid-map-sequence-id-378103

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Showing: 1 - 40 of 234 results