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Showing: 1 - 50 of 51 results

  2. pCS2+-HypocratesCS

    Plasmid
    #161960
    Purpose
    Mammalian expression of non-targeted inactivated control version of genetically encoded biosensor for (pseudo)hypohalous acids and their derivatives
    Depositor
    Dmitry Bilan
    Article
    Kostyuk et al Nat Commun. 2022 Jan 10;13(1):171. doi:
    Insert
    Hypocrates
    Use
    Tags
    Expression
    Mammalian
    Mutation
    changed Cysteine 355 to Serine
    Promoter
    CMV, SP6
    Available since
    Feb. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    161960-plasmid-map-sequence-id-324303

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  3. pQE30-HypocratesCS

    Plasmid
    #161957
    Purpose
    Bacterial expression of inactivated control version of genetically encoded biosensor for (pseudo)hypohalous acids and their derivatives
    Depositor
    Dmitry Bilan
    Article
    Kostyuk et al Nat Commun. 2022 Jan 10;13(1):171. doi:
    Insert
    HypocratesCS
    Use
    Tags
    6xHIs tag
    Expression
    Bacterial
    Mutation
    changed cysteine 355 to serine
    Promoter
    T5
    Available since
    May 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    161957-plasmid-map-sequence-id-324311

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  4. pC5Kan-P2A

    Plasmid
    #51814
    Purpose
    P2A peptide from porcine teschovirus-1 flanked by restriction sites in shuttle vector for easy cloning of multiple separate proteins to be expressed from single message
    Depositor
    Barry Ganetzky
    Article
    Daniels et al PLoS One. 2014 Jun 19;9(6):e100637. doi:
    Insert
    P2A peptide (PTV1gp1 Synthetic, porcine teschovirus-1)
    Use
    Shuttle vector for insect expression vectors
    Tags
    Expression
    Mutation
    codons altered for Drosophila and to eliminate re…
    Promoter
    Available since
    June 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    51814-plasmid-map-sequence-id-79554

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  6. pmCherry-C1 R62D actin-3XNLS P2A mCherry

    Plasmid
    #58477
    Purpose
    Expresses nuclear-targeted non-polymerizing R62D mutant of human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter
    Depositor
    Dyche Mullins
    Article
    Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife
    Inserts
    beta actin (ACTB Human)
    3XNLS
    P2A cleavage site
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Changed Arginine 62 to Aspartic Acid
    Promoter
    CMV
    Available since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    58477-plasmid-map-sequence-id-90386

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  9. FUG-P2A-tC3-W

    Plasmid
    #129475
    Purpose
    3rd-generation lentiviral vector for hUbC-driven co-expression of EGFP and truncated C3
    Depositor
    Robert Gross
    Insert
    truncated (AA173-201) exoenzyme C3 from C. botulinum, P2A, EGFP
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human ubiquitin C
    Available since
    Feb. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    129475-plasmid-map-sequence-id-384588

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  10. pAAV/CBA::EGFP-P2A-tC3

    Plasmid
    #129476
    Purpose
    AAV2 transfer plasmid for truncated C3 with EGFP under control of the CBA promoter
    Depositor
    Robert Gross
    Insert
    truncated (AA173-201) exoenzyme C3 from C. botulinum, P2A, EGFP
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CBA
    Available since
    Feb. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    129476-plasmid-map-sequence-id-384590

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  12. pAAV/CBA::EGFP-P2A-myc-C3

    Plasmid
    #129478
    Purpose
    AAV2 transfer plasmid for myc-tagged C3 with EGFP under control of the CBA promoter
    Depositor
    Robert Gross
    Insert
    Myc-tagged (N-terminus) exoenzyme C3 from C. botulinum, P2A, EGFP
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CBA
    Available since
    Feb. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    129478-plasmid-map-sequence-id-384594

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  14. pAAV/CBA::EGFP-P2A-tC3/54aa-FLAG

    Plasmid
    #129479
    Purpose
    AAV2 transfer plasmid for truncated C3 (54 aa) tagged with FLAG with EGFP under control of the CBA promoter
    Depositor
    Robert Gross
    Insert
    truncated (AA148-201) exoenzyme C3 from C. botulinum, P2A, EGFP
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CBA
    Available since
    Feb. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    129479-plasmid-map-sequence-id-384595

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  15. pAAV/CBA::EGFP-P2A-tC3-FLAG

    Plasmid
    #129477
    Purpose
    AAV2 transfer plasmid for truncated C3 tagged with FLAG with EGFP under control of the CBA promoter
    Depositor
    Robert Gross
    Insert
    FLAG-tagged (C-terminus) truncated (AA173-201) exoenzyme C3 from C. botulinum, P2A, EGFP
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CBA
    Available since
    Feb. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    129477-plasmid-map-sequence-id-384592

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  23. pYTK001_P2Ab

    Plasmid
    #183084
    Purpose
    2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use in bi-cistronic and as the first 2A peptide in tri-cistronic construct assembly
    Depositor
    Zhen Wang
    Article
    Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.
    Insert
    P2A
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    183084-plasmid-map-sequence-id-367243

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  31. pYTK001_P2At(2nd)

    Plasmid
    #183087
    Purpose
    2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in tri-cistronic and third 2A peptide in quad-cistronic construct assembly
    Depositor
    Zhen Wang
    Article
    Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.
    Insert
    P2A
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    183087-plasmid-map-sequence-id-367246

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  44. pDEW8

    Plasmid
    #84238
    Purpose
    Plasmid containing a P2A peptide (porcine teschovirus-1 derived) sequence that works efficiently in Saccharomyces cerevisiae
    Depositor
    David Weinberg
    Article
    DiSanto et al Elife. 2016 Oct 1;5. pii: e20069. doi: 1
    Insert
    HA-mRuby-P2A-eGFP
    Use
    Tags
    HA-mRuby and eGFP
    Expression
    Yeast
    Mutation
    Promoter
    GPD
    Available since
    Nov. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    84238-plasmid-map-sequence-id-164972

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  49. pEGFP_D2A_tdTomato

    Plasmid
    #184045
    Purpose
    Expresses EGFP and tdTomato in mammalian cells under control of a CMV promoter by using a dual 2A peptide sequence (P2AT2A)
    Depositor
    Angela Pannier
    Article
    Kozisek et al J Biol Eng. 2023 Dec 7;17(1):76. doi: 10
    Inserts
    Enhanced Green Fluorescent Protein
    tdTomato
    Dual 2A Peptide Sequence
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    184045-plasmid-map-sequence-id-363159

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Showing: 1 - 50 of 51 results