We narrowed to 1,639 results for: plasmid for e coli

1. pXTK056 [Con-Pro_glpT-Ecoli_PartType8]

   Plasmid

   #229293

   Purpose

   XanthoMoClo Golden Gate Part Plasmid for cloning, contains Constitutive-Promoter_glpT-Ecoli_PartType8

   Depositor

   Michael Springer

   Article

   Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

   Insert

   glpT Promoter (E coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pXTK037 [Con-Pro_glpT-Ecoli_PartType5]

   Plasmid

   #229274

   Purpose

   XanthoMoClo Golden Gate Part Plasmid for cloning, contains Constitutive-Promoter_glpT-Ecoli_PartType5

   Depositor

   Michael Springer

   Article

   Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

   Insert

   glpT Promoter (E coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. MP-E

   Plasmid

   #69636

   Purpose

   Untargetted Bacterial Mutagenesis

   Depositor

   David Liu

   Article

   Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

   Insert

   dnaQ926 dnaX36
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   E. coli PBAD

   Available since

   Sept. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Plasmid EUPp_09

   Plasmid

   #157745

   Purpose

   PgyrA_ada _adh2 _pMB1_ampR (for expressing two enzymes (Ada and Adh2) to allow E. coli to consume ethanol)

   Depositor

   Kang Zhou

   Article

   Liang et al Metab Eng. 2020 Nov 25. pii: S1096-7176(

   Inserts

   acetaldehyde dehydrogenase
   alcohol dehydrogenase (ADH2 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PgyrA

   Available since

   June 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Tn7 helper plasmid

   Plasmid

   #141161

   Purpose

   Temperature-sensitive plasmid carries tnsABCD genes that encode transposase biochemical machinery under an arabinose-inducible pBAD promoter

   Depositor

   Shimon Bershtein

   Article

   Jasinska et al Nat Ecol Evol. 2020 Feb 24. pii: 10.1038

   Insert

   Tn7 transposon machinery
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Arabinose-inducible pBAD

   Available since

   Aug. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Tn7 integration plasmid

   Plasmid

   #154134

   Purpose

   Suicidal integration plasmid with the Tn7 arms, streptomycin and kanamycin resistance genes and the restriction site to clone the barcode-carrying cassette.

   Depositor

   Shimon Bershtein

   Article

   Jasinska et al Nat Ecol Evol. 2020 Feb 24. pii: 10.1038

   Inserts

   left and right end of Tn7 arm
   Spectinomycin resistant gene
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Spectinomycin resistance gene promoter and no

   Available since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pETM41-EcPPK

   Plasmid

   #38334

   Depositor

   Florian Freimoser

   Insert

   E. coli polyphosphate kinase (ppk Escherichia coli)
   

   Use

   Tags

   6xHIS, MBP, and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pKM263-EcPPK

   Plasmid

   #38333

   Depositor

   Florian Freimoser

   Insert

   E. coli polyphosphate kinase (ppk Escherichia coli)
   

   Use

   Tags

   6xHIS, GST, and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTCF194 Mamu-E*02:04

   Plasmid

   #180461

   Purpose

   E. coli expression of soluble MHC protein

   Depositor

   John Altman

   Insert

   Mamu-E*02:04
   

   Use

   Tags

   BSP41

   Expression

   Bacterial

   Mutation

   Replacement of transmembrane domain with enzymati…

   Promoter

   Available since

   Feb. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGU08

    Plasmid

    #212149

    Purpose

    Plasmid expressing catalase-peroxidase and D-amino acid dehydrogenase under constitutive promoter PgyrA control. Best E. coli glycine utilization plasmid.

    Depositor

    Kang Zhou

    Insert

    catalase-peroxidase, D-amino acid dehydrogenase (katG katG: E. coli, dadA6: Cupriavidus necator)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PgyrA

    Available since

    March 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pM2s2TsR

    Plasmid

    #137923

    Purpose

    E. coli Nissle pMUT2-derived plasmid with tetracycline resistance and constitutive RFP expression

    Depositor

    Neel Joshi

    Article

    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

    Insert

    mRFP1
    

    Use

    Synthetic Biology; For use with e. coli nissle in…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pM1s3TsR

    Plasmid

    #137922

    Purpose

    E. coli Nissle pMUT1-derived plasmid with tetracycline resistance and constitutive RFP expression

    Depositor

    Neel Joshi

    Article

    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

    Insert

    mRFP1
    

    Use

    Synthetic Biology; For use with e. coli nissle in…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET30a-Spike-E

    Plasmid

    #165096

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 spike protein (S SARS-CoV2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 11, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pmr101A-cPr-mCherry (KmR)

    Plasmid

    #177207

    Purpose

    Plasmid with p15A origin for constitutive mCherry expression in E. coli

    Depositor

    Alexey Merz

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Synthetic constitutive promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pmr101A-cPr-mCherry (ErmR)

    Plasmid

    #177206

    Purpose

    Plasmid with p15A origin for constitutive mCherry expression in E. coli

    Depositor

    Alexey Merz

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Synthetic constitutive promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pM1s3AsG

    Plasmid

    #137921

    Purpose

    E. coli Nissle pMUT1-derived plasmid with ampicillin resistance and constitutive GFP expression

    Depositor

    Neel Joshi

    Article

    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

    Insert

    Superfolder GFP
    

    Use

    Synthetic Biology; For use with e. coli nissle in…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pM2s2AsG

    Plasmid

    #137924

    Purpose

    E. coli Nissle pMUT2-derived plasmid with ampicillin resistance and constitutive GFP expression

    Depositor

    Neel Joshi

    Article

    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

    Insert

    Superfolder GFP
    

    Use

    Synthetic Biology; For use with e. coli nissle in…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101

    Available since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pM1s3ATScsg-Etag

    Plasmid

    #137946

    Purpose

    E. coli Nissle pMUT1-derived plasmid with ampicillin resistance and temperature sensitive curli expression

    Depositor

    Neel Joshi

    Article

    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

    Inserts

    Synthetic curli operon
    Synthetic curli operon
    

    Use

    Synthetic Biology; For use with e. coli nissle in…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTlpA36

    Available since

    April 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pM1s3AsR_TS-C7

    Plasmid

    #137945

    Purpose

    E. coli Nissle pMUT1-derived plasmid with ampicillin resistance and constitutive RFP expression and strong temperature sensitive GFP expression

    Depositor

    Neel Joshi

    Article

    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

    Insert

    mRFP1
    

    Use

    Synthetic Biology; For use with e. coli nissle in…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101

    Available since

    April 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGBW-m4046814

    Plasmid

    #145601

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. pGBW-m4046945

    Plasmid

    #145668

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. pGBW-m4046730

    Plasmid

    #145659

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;HA

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. pM2s2AsR_TS-E10

    Plasmid

    #137944

    Purpose

    E. coli Nissle pMUT2-derived plasmid with ampicillin resistance and constitutive RFP expression and strong temperature sensitive GFP expression

    Depositor

    Neel Joshi

    Article

    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

    Inserts

    mRFP1
    mWasabi
    

    Use

    Synthetic Biology; For use with e. coli nissle in…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101 and pTlpA-C7

    Available since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pmr101A-cPr-mNeonGreen (KmR)

    Plasmid

    #177209

    Purpose

    Plasmid with p15A origin for constitutive mNeonGreen expression in E. coli

    Depositor

    Alexey Merz

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Yeast codon optimized

    Promoter

    Synthetic constitutive promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pmr101A-cPr-mNeonGreen (ErmR)

    Plasmid

    #177208

    Purpose

    Plasmid with p15A origin for constitutive mNeonGreen expression in E. coli

    Depositor

    Alexey Merz

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Yeast codon optimized

    Promoter

    Synthetic constitutive promoter

    Available since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGBW-m4046768

    Plasmid

    #145763

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. pGBW-m4046949

    Plasmid

    #145604

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;FLAG_2

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pGBW-m4046728

    Plasmid

    #145739

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pGBW-m4046729

    Plasmid

    #145653

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;HA

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pGBW-m4046935

    Plasmid

    #145580

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;Myc

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. pSLQ1651-sgRNA(F+E)-sgGal4

    Plasmid

    #100549

    Purpose

    sgGal4-expressing vector modified from Addgene plasmid #51024

    Depositor

    Jian Xu

    Article

    Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

    Insert

    sgGal4
    

    Use

    CRISPR

    Tags

    sgRNA

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pGBW-m4136463

    Plasmid

    #149527

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    6xHIS;Linker_GGSG;SUMO tag;Linker_GGSG;Cleavable …

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. pGBW-m4136460

    Plasmid

    #149532

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    6xHIS;Linker_GGSG;MBP;Linker_GGSG;Cleavable TEV

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. pGBW-m4136453

    Plasmid

    #149535

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    6xHIS;Linker_GGSG;GST;Linker_GGSG;Cleavable TEV

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. pRS415-GPD-PPK

    Plasmid

    #183941

    Purpose

    Expresses E. coli PPK from yeast GPD promoter

    Depositor

    Michael Downey

    Insert

    PPK (ppk E. coli)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    WT

    Promoter

    GPD

    Available since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET15-EF1p_mFAP2b

    Plasmid

    #159469

    Purpose

    Encodes for EF1p_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.

    Depositor

    David Baker

    Insert

    EF1p_mFAP2b
    

    Use

    Tags

    6xHis-thrombin-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET15-EF4n_mFAP2b

    Plasmid

    #159464

    Purpose

    Encodes for EF4n_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.

    Depositor

    David Baker

    Insert

    EF4n_mFAP2b
    

    Use

    Tags

    6xHis-thrombin-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET15-EF1p3_mFAP2b

    Plasmid

    #159470

    Purpose

    Encodes for EF1p3_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.

    Depositor

    David Baker

    Insert

    EF1p3_mFAP2b
    

    Use

    Tags

    6xHis-thrombin-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET15-mFAP10

    Plasmid

    #159463

    Purpose

    Encodes for mFAP10 with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.

    Depositor

    David Baker

    Insert

    mFAP10
    

    Use

    Tags

    6xHis-thrombin-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET15-EF2n3_mFAP2b

    Plasmid

    #159466

    Purpose

    Encodes for EF2n3_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.

    Depositor

    David Baker

    Insert

    EF2n3_mFAP2b
    

    Use

    Tags

    6xHis-thrombin-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pET15-EF1n2_mFAP2b

    Plasmid

    #159471

    Purpose

    Encodes for EF1n2_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.

    Depositor

    David Baker

    Insert

    EF1n2_mFAP2b
    

    Use

    Tags

    6xHis-thrombin-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pET15-EF1n3_mFAP2b

    Plasmid

    #159472

    Purpose

    Encodes for EF1n3_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.

    Depositor

    David Baker

    Insert

    EF1n3_mFAP2b
    

    Use

    Tags

    6xHis-thrombin-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pET15-EF1n_mFAP2b

    Plasmid

    #159473

    Purpose

    Encodes for EF1n_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.

    Depositor

    David Baker

    Insert

    EF1n_mFAP2b
    

    Use

    Tags

    6xHis-thrombin-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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44. pET15-EF2n_mFAP2b

    Plasmid

    #159468

    Purpose

    Encodes for EF2n_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.

    Depositor

    David Baker

    Insert

    EF2n_mFAP2b
    

    Use

    Tags

    6xHis-thrombin-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pET15-EF2n2_mFAP2b

    Plasmid

    #159467

    Purpose

    Encodes for EF2n2_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.

    Depositor

    David Baker

    Insert

    EF2n2_mFAP2b
    

    Use

    Tags

    6xHis-thrombin-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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46. pKPY115

    Plasmid

    #72669

    Purpose

    Encodes Thr251Gly-EcPheRS Under Arabinose-Inducible (PBAD) Control

    Depositor

    David Tirrell

    Insert

    Thr251Gly-EcPheRS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Thr251Gly in Alpha Subunit

    Promoter

    PBAD

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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47. INT_Cargo_PgyrA_katG_dadA6

    Plasmid

    #212142

    Purpose

    Cargo plasmid for integrating PgyrA expression cassette of catalase-peroxidase and D-amino acid dehydrogenase into the genome. Plasmid can be removed by incubating cells at 37 C.

    Depositor

    Kang Zhou

    Insert

    catalase-peroxidase, D-amino acid dehydrogenase (katG katG: E. coli, dadA6: Cupriavidus necator)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PgyrA

    Available since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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48. p416-GPD-HA-PPK

    Plasmid

    #183943

    Purpose

    Expresses HA-tagged E. coli PPK from yeast GPD promoter

    Depositor

    Michael Downey

    Insert

    PPK (ppk E. coli)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    WT

    Promoter

    GPD

    Available since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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49. p416-GPD-PPK(mutant)

    Plasmid

    #183942

    Purpose

    Expresses catalytic mutant of E. coli PPK from yeast GPD promoter

    Depositor

    Michael Downey

    Insert

    PPK(mutant) (ppk E. coli )
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    mutations in His435, His454, and His592 to Alanine

    Promoter

    GPD

    Available since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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50. PCDF Bravo-PotH-OL1del

    Plasmid

    #216749

    Purpose

    Study the interaction of PotH in E. coli, specifically focusing on the role of the outer loop 1 (OL1) by examining the variant lacking OL1 in its interaction with exogenous peptides.

    Depositor

    Gordon Laurie

    Insert

    potH (potH E. coli (Bacteria))
    

    Use

    Tags

    10x His

    Expression

    Bacterial

    Mutation

    Outer loop 1 (OL1) with the sequence of [FKISLAEM…

    Promoter

    Available since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge