Addgene

We narrowed to 867 results for: GGCT;

Showing: 271 - 300 of 867 results

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pSV_207

Plasmid

#122938

Purpose

Expression of the marked mRNA under the control of substitutional promoters.

Depositor

Attila Becskei

Article

Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

Insert

YML070W (Marked)

Use

Tags

Expression

Yeast

Mutation

YML070W: bp 52-63 GGGTTTGCCCTT to GGCTTCGCGCTA

Promoter

[tetO2]4inPgal1

Available since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pDEAF1.2.2-gDNA

Plasmid

#113799

Purpose

CRISPR plasmid for expression of Cas9 nicakse (D10A) and gRNA targeting human transcription factor DEAF1

Depositor

Kevin White

Insert

DEAF1 (DEAF1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pT2K-CAGGS-U6-sgRNA-M5-IRES-CFP

Plasmid

#114728

Purpose

sgRNA targeting a sequence upstream of the initiator ATG of the cellular Myc gene

Depositor

Martine Roussel

Article

Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1

Insert

sgRNA M5

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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PX458_ZSCAN9_1

Plasmid

#86312

Purpose

Encodes gRNA for 3' target of human ZSCAN9

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against ZSCAN9 (ZNF193 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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CRISPR_HLA_class_I_2

Plasmid

#164987

Purpose

Expression of gRNA targeting multiple HLA-A, HLA-B, and HLA-C genes

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

gRNA against HLA class I

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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7b sgRNA for EJ7-GFP reporter

Plasmid

#113624

Purpose

sgRNA/CAS9 expression plasmid to induce the 3’ double-strand break in the EJ7-GFP reporter

Depositor

Jeremy Stark

Article

Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

Insert

7b sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only
7a sgRNA for EJ7-GFP and 4-μHOM reporters

Plasmid

#113620

Purpose

sgRNA/CAS9 expression plasmid to induce the 5’ double-strand break in both the EJ7-GFP and 4-μHOM reporters

Depositor

Jeremy Stark

Article

Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

Insert

7a sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only
pC0052-REPAIR non-targeting guide clone into pC0043

Plasmid

#103868

Purpose

Non-targeting guide RNA for use with PspCas13b. Useful as a control for RNA editing and RNA knockdown experiments.

Depositor

Feng Zhang

Article

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Insert

non-targeting guide RNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pLG1-puro Non-targeting sgRNA 3

Plasmid

#109003

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

Non-targeting sgRNA #3

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6 promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO-shSmad4

Plasmid

#37046

Depositor

Sam Thiagalingam

Article

Papageorgis et al Cancer Res. 2010 Feb 1;70(3):968-78. Epu

Insert

Smad4 (SMAD4 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 18, 2013

Availability

Academic Institutions and Nonprofits only
pLentiCRISPRv2-TRAF6-targeting sgRNA 2

Plasmid

#131346

Purpose

gRNA targeting mouse TRAF6

Depositor

Jonathan Kagan

Article

Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

Insert

gRNA targeting mouse TRAF6 (Traf6 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pG3H-U6SC

Plasmid

#134755

Purpose

pGreen3 CRISPR/Cas9

Depositor

Qi-Jun Chen

Article

Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

Insert

CRISPR/Cas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

gRNA-U6, zCas9-35SC

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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PX458_TEAD1_1

Plasmid

#86356

Purpose

Encodes gRNA for 3' target of human TEAD1

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against TEAD1 (TEAD1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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T5-sgRNA

Plasmid

#113655

Purpose

sgRNA targeting unit plasmid. The sgRNA targeting unit plasmids contain connector ConLS, ConR1, and one sgRNA transcriptional unit.

Depositor

Jeffrey Barrick

Article

Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531

Insert

promoter T5 and sgRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

T5

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pG3K-U6SC

Plasmid

#134754

Purpose

pGreen3 CRISPR/Cas9

Depositor

Qi-Jun Chen

Article

Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

Insert

CRISPR/Cas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

gRNA-U6, zCas9-35SC

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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2X_pX458_pSpCas9(BB)-2A-GFP_SOX17_bKO

Plasmid

#172225

Purpose

Cas9-2A-GFP expression vector bearing two sgRNAs targeting the centromeric human SOX17 CTCF-boundary.

Depositor

Alexander Meissner

Article

Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

Insert

sgRNA

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Mutation

Promoter

Cbh

Available since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-A2NT

Plasmid

#62257

Purpose

expression of A2NT sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

A2NT sgRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pG3B-U6SC

Plasmid

#134753

Purpose

pGreen3 CRISPR/Cas9

Depositor

Qi-Jun Chen

Article

Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

Insert

CRISPR/Cas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

gRNA-U6, zCas9-35SC

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pG3B-U3Ub

Plasmid

#134750

Purpose

pGreen3 CRISPR/Cas9

Depositor

Qi-Jun Chen

Article

Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

Insert

CRISPR/Cas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

gRNA- OsU3, zCas9-Ubi1

Available since

Dec. 12, 2019

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-sgGOT1

Plasmid

#72874

Purpose

The lentiviral sgGOT1 vector was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEB).

Depositor

David Sabatini

Article

Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

Insert

sgGOT1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 4, 2016

Availability

Academic Institutions and Nonprofits only
CROP_C9_Puro_CSF1R

Plasmid

#183277

Purpose

All-in-One CRISPRko system with a guide RNA that targets CSF1R gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

CSF1R

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pOC3 smFP-HA-Cacna1e KI

Plasmid

#183436

Purpose

CreON FlpOFF knock-in for smFP-HA-CaV2.3 (amino acid position: G5)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and smFP HA donor

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pegRNA_HEK4_G-T

Plasmid

#201975

Purpose

Express a pegRNA used for G to T conversion at HEK4 site

Depositor

Scot Wolfe

Article

Liang et al Nat Methods. 2023 Jun;20(6):898-907. doi

Insert

pegRNA_HEK4_G-T

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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pegRNA_HEK4_1bp-deletion

Plasmid

#201976

Purpose

Express a pegRNA used for 1bp deletion at HEK4 site

Depositor

Scot Wolfe

Article

Liang et al Nat Methods. 2023 Jun;20(6):898-907. doi

Insert

pegRNA_HEK4

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR - DDX3Y sgRNA 2

Plasmid

#70657

Purpose

Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a DDX3Y targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

sgRNA against DDX3Y

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only
1154B_gFLE_pB_VTK_ActG

Plasmid

#187238

Purpose

An. gambiae transgenesis plasmid. PiggyBac with transposase in backbone. Expresses two gRNAs targeting fle

Depositor

Omar Akbari

Article

Smidler et al Sci Adv. 2023 Jul 7;9(27):eade8903. doi:

Insert

Femaleless

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-scramble

Plasmid

#62285

Purpose

expression of scramble sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

scramble sgRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pG3H-U6EC1

Plasmid

#134758

Purpose

pGreen3 CRISPR/Cas9

Depositor

Qi-Jun Chen

Article

Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

Insert

CRISPR/Cas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

gRNA-U6, zCas9-EC1

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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CAG-mCherry-CrxshRNA

Plasmid

#73987

Purpose

Crx shRNA (mir30 backbone) was inserted into the 3'UTR of mCherry.

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

shRNA that targets the mouse Crx gene

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-shNup35-EcoRI

Plasmid

#87332

Purpose

To express shRNA against human Nup35

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup35

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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