We narrowed to 9,122 results for: Pol

1. mDlx-BiPOLES-mCerulean

   Plasmid

   #154946

   Purpose

   Optogenetic tool for blue-light inhibition and red-light excitation of neurons

   Depositor

   Simon Wiegert

   Article

   Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

   Insert

   BiPOLES
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   minimal Dlx

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pOT2-poly-cis

   Plasmid

   #141177

   Purpose

   To clone any gene or STTM sequence or artificial miRNA under CaMV 35S promoter

   Depositor

   Guiliang Tang

   Type

   Empty backbone

   Use

   RNAi and Synthetic Biology

   Expression

   Plant

   Promoter

   CaMV 35S promoter

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pVENUS-N1-POLR2G-GFP

   Plasmid

   #86872

   Purpose

   To express POLR2G (RPB7) tagged with VENUS at C terminus.

   Depositor

   Yihong Ye

   Article

   Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

   Insert

   POLR2G (POLR2G Human)
   

   Tags

   VENUS

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. HO-pMET-poly-KanMX4-HO

   Plasmid

   #51665

   Purpose

   yeast plasmid for integration of target sequence at HO locus, Kan selection, pMET inducible promoter

   Depositor

   David Stillman

   Article

   Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

   Type

   Empty backbone

   Use

   Yeast integration

   Expression

   Yeast

   Available Since

   June 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV6-AN-DDK_UBM1*2* POLI

   Plasmid

   #131232

   Purpose

   Mammalian expression of an N-terminally FLAG-tagged DNA polymerase iota mutant, containing point mutations in both UBM domains

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   DNA polymerase iota (POLI Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains inactivating mutations in both ubiquitin…

   Promoter

   CMV enhancer + CMV promoter

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFIV-H1-Puro-hPolß

   Plasmid

   #18663

   Depositor

   Robert Sobol

   Article

   Trivedi et al Molecular Pharmacology 2008;74(2):505-16

   Insert

   shRNA specific to human DNA polymerase beta (POLB Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Mutation

   none

   Available Since

   Oct. 24, 2008

   Availability

   Academic Institutions and Nonprofits only

7. attB-mCh-Poly(A)

   Plasmid

   #65525

   Purpose

   Plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

   Depositor

   Sebastian Bultmann

   Article

   Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

   Insert

   attB-mCherry
   

   Use

   Mouse Targeting; Bxb1

   Expression

   Mammalian

   Available Since

   Aug. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. gRNA-POLQ-Halo

   Plasmid

   #211636

   Purpose

   sgRNA against POLQ

   Depositor

   Agnel Sfeir

   Article

   Brambati et al Science. 2023 Aug 11;381(6658):653-660.

   Insert

   sgRNA POLQ
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AAV_Actb HMEJ donor_U6_sgRNA_EF1a_GFP_polyA

   Plasmid

   #97308

   Purpose

   HMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

   Depositor

   Hui Yang

   Article

   Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

   Insert

   Actb HMEJ donor
   

   Use

   AAV and Mouse Targeting

   Expression

   Mammalian

   Promoter

   U6, EF1a

   Available Since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEX4T3-GST-PolB(WT)

    Plasmid

    #177131

    Purpose

    Bacterial vector for expression of an N-terminal GST fusion of PolB(WT) with a TEV protease site located between the GST tag and PolB(WT)

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    PolB (POLB Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    Tac

    Available Since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. attB-­GFP-­Poly(A)

    Plasmid

    #65524

    Purpose

    Plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    attB-GFP
    

    Use

    Mouse Targeting; Bxb1

    Expression

    Mammalian

    Available Since

    Aug. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AAV_Actb MMEJ donor_U6_sgRNA_EF1a_GFP_polyA

    Plasmid

    #97311

    Purpose

    MMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    Actb MMEJ donor
    

    Use

    AAV and Mouse Targeting

    Expression

    Mammalian

    Promoter

    U6, EF1a

    Available Since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pESC-URA-hPOLISc

    Plasmid

    #60819

    Purpose

    to express human Pol iota recoded to yeast codons in yeast

    Depositor

    Youri Pavlov

    Article

    Makarova et al PLoS One. 2011 Jan 31;6(1):e16612. doi:

    Insert

    POLI (POLI Human)
    

    Tags

    c-myc

    Expression

    Yeast

    Mutation

    optimized to yeast codon usage

    Promoter

    GAL1

    Available Since

    March 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDONR221 Cas9-POLD3

    Plasmid

    #183197

    Purpose

    Entry clone

    Depositor

    Emma Haapaniemi

    Article

    Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

    Insert

    Cas9-POLD3
    

    Use

    CRISPR; Gateway entry clone

    Mutation

    n/a

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. FRB-polySH33-EGFP

    Plasmid

    #175792

    Purpose

    Bacterial expression and purification, fluorescently tagged scaffold for droplet visualization and volume quantification

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    NCK1 (NCK1 Human)
    

    Tags

    His6 and MBP

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Lenti_Actb HMEJ donor_U6_sgRNA_EF1a_GFP_polyA

    Plasmid

    #97312

    Purpose

    HMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. Lenti backbone.

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    Actb HMEJ donor
    

    Use

    Lentiviral and Mouse Targeting

    Expression

    Mammalian

    Promoter

    U6, EF1a

    Available Since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBSc polyA + Shal1

    Plasmid

    #16196

    Depositor

    Larry Salkoff

    Article

    Wei et al Science. 1990 May 4. 248(4955):599-603.

    Insert

    Drosophila melanogaster Shaker cognate l (Shal Fly)
    

    Use

    Xenopus oocyte expression

    Mutation

    N/A

    Available Since

    Jan. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

18. Sumo3 PolQM1 deltains1

    Plasmid

    #78461

    Purpose

    Inducible expression in E. coli

    Depositor

    Susan Wallace, Sylvie Doublie

    Article

    Hogg et al J Mol Biol. 2011 Jan 21;405(3):642-52. d

    Insert

    Polymerase theta (POLQ Human)
    

    Tags

    His and SUMO3

    Expression

    Bacterial

    Mutation

    delta 1-1791; delta 2149-2170; Q2513R (please see…

    Promoter

    T7

    Available Since

    July 28, 2016

    Availability

    Academic Institutions and Nonprofits only

19. GFP-LacI-polylinker

    Plasmid

    #179271

    Purpose

    Cloning of GFP-LacI fusion proteins for LacI/LacO tethering

    Depositor

    Lienhard Schmitz

    Article

    Haas et al Front Cell Dev Biol. 2020 Sep 1;8:852. d

    Type

    Empty backbone

    Tags

    GFP and LacI

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HB901: pMVP (L3-L2) polyA

    Plasmid

    #121746

    Purpose

    pMVP L3-L2 entry plasmid, contains human growth hormone polyadenylation signal (polyA) for 3- or 4-component MultiSite Gateway Pro assembly

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    Polyadenylation signal (polyA)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge