We narrowed to 9,257 results for: Coli

1. pDOC-K-glmS-lacIZ

   Plasmid

   #158061

   Purpose

   Donor plasmid for insertion of the lacIZ operon and lac promoter into the S. Typhimurium chromosome

   Depositor

   Mark Webber

   Article

   Holden et al Microbiology (Reading). 2020 Nov 23. doi

   Insert

   lacIZ operon
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCYB-TnsA

   Plasmid

   #17356

   Depositor

   Nancy Craig

   Article

   Hickman et al Mol Cell. 2000 Jun . 5(6):1025-34.

   Insert

   TnsA (tnsA E. coli transposon Tn7)
   

   Use

   Tags

   Intein-Chitin binding domain

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

3. pRSET FLIPglu-170nDelta13

   Plasmid

   #13562

   Depositor

   Wolf Frommer

   Article

   Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

   Insert

   FLIPglu F16F Delta13
   

   Use

   Tags

   ECFP and EYFP

   Expression

   Bacterial

   Mutation

   WT binding protein (no F16A)

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGK415-­rtetTAK8N, L131L

   Plasmid

   #165969

   Purpose

   Expresses rTetTA (K8N, L131L) mutant

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

   Insert

   tetR
   

   Use

   Synthetic Biology

   Tags

   three copies of VP16 (VP48)

   Expression

   Yeast

   Mutation

   Promoter

   pPGK1

   Available since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHelper_O

   Plasmid

   #173783

   Purpose

   The 186 helper plasmid necessary for genomic integration of the pIT5_KO plasmid.

   Depositor

   Keith Shearwin

   Article

   Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

   Insert

   186 Integrase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   E59G - please see depositor comments

   Promoter

   Available since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHelper_W

   Plasmid

   #173784

   Purpose

   The Wphi helper plasmid necessary for genomic integration of the pIT5_KW plasmid.

   Depositor

   Keith Shearwin

   Article

   Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

   Insert

   Wphi Integrase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHelper_D

   Plasmid

   #173781

   Purpose

   The D145 helper plasmid necessary for genomic integration of the pIT5_KD plasmid.

   Depositor

   Keith Shearwin

   Article

   Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

   Insert

   D145 Integrase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TDGS50

   Plasmid

   #183189

   Purpose

   TEV protease expressed under J23106 promoter; DivIVAsfGFP-TEV protease recognition site-M0050 expressed under J23116 promoter

   Depositor

   Hsiao-Chun Huang

   Article

   Hong et al ACS Synth Biol. 2021 Aug 20;10(8):1830-1

   Inserts

   DivIVA-GFP(tevR M0050)
   TEV-S219V
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJAT13

   Plasmid

   #18986

   Depositor

   Jay Keasling

   Article

   Khlebnikov et al Microbiology. 2001 Dec . 147(Pt 12):3241

   Insert

   promoter CP13
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pGR-ECK120015170

    Plasmid

    #46006

    Depositor

    Christopher Voigt

    Article

    Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

    Insert

    ECK120015170 Terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p15aC-4D1D

    Plasmid

    #107864

    Purpose

    Bacterial expression of PIS and PI4K

    Depositor

    Sanford Simon

    Article

    Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

    Inserts

    PIS
    phosphatidylinositol 4-kinase β
    

    Use

    Tags

    myc

    Expression

    Bacterial

    Mutation

    Promoter

    proD

    Available since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSEL2

    Plasmid

    #23012

    Depositor

    James Bowie

    Article

    Massey-Gendel et al Protein Sci. 2009 Feb . 18(2):372-83.

    Insert

    Kanamycin resistance
    

    Use

    Tags

    6 His Tag and Xpress epitope

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pKS043

    Plasmid

    #65471

    Purpose

    pTrc plasmid expressing clpS

    Depositor

    Keith Tyo

    Article

    Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

    Insert

    clpS (clpS )
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSELt_S7.A

    Plasmid

    #125835

    Purpose

    Negative selection plasmid for evolution of an amber stop codon suppression tRNA

    Depositor

    Andrew Ellington

    Article

    Maranhao et al ACS Synth Biol. 2017 Jan 20;6(1):108-119

    Insert

    ACP-2xAmb-pheS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pr.trpL*

    Available since

    May 15, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pZH509

    Plasmid

    #102664

    Purpose

    Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

    Depositor

    Zach Hensel

    Article

    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

    Insert

    GFPmut2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBP-TL4

    Plasmid

    #72988

    Purpose

    RBS_H5

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    H5 RBS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pBP-TL8

    Plasmid

    #72992

    Purpose

    RBS_L1

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    L1 RBS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pBP-L1U1H09

    Plasmid

    #73008

    Purpose

    L1U1H09 terminator

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    L1U1H09 terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. p3XFlag-YedK

    Plasmid

    #84567

    Purpose

    Expresses YedK in mammalian cells

    Depositor

    Douglas Feldman

    Article

    Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

    Insert

    YedK
    

    Use

    Tags

    3xFlag and C-Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAK02

    Plasmid

    #19001

    Depositor

    Jay Keasling

    Article

    Khlebnikov et al J Bacteriol. 2000 Dec . 182(24):7029-34.

    Insert

    arabinose transporter araE under control of Ptac (araE E. coli)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

21. pBEL1324

    Plasmid

    #139874

    Purpose

    Expression plasmid for WT LetB (without TM region) for cryo and negative stain EM

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetB(43-877)-6xHis

    Promoter

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGR-ECK120010783

    Plasmid

    #46009

    Depositor

    Christopher Voigt

    Article

    Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

    Insert

    ECK120010783 Terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    June 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDUET-ctCPR-nAMO

    Plasmid

    #20110

    Depositor

    Jay Keasling

    Article

    Chang et al Nat Chem Biol. 2007 May . 3(5):274-7.

    Insert

    p450 reductase and native Amorphadiene oxidase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

24. pGR-ECK125108723

    Plasmid

    #46008

    Depositor

    Christopher Voigt

    Article

    Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

    Insert

    ECK125108723 Terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    June 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSB1A3-AD010

    Plasmid

    #116851

    Purpose

    Expresses araC constitutively. Expresses alpha-hemolysin and mScarletI (RFP) under a pBAD promoter; alternate name pSB1A2-AD010

    Depositor

    Friedrich Simmel

    Article

    Dupin et al Nat Chem. 2018 Nov 26. pii: 10.1038/s415

    Inserts

    AraC
    alpha-HL
    mScarletI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pKS012

    Plasmid

    #65465

    Purpose

    pSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) Ntag and mCherry

    Depositor

    Keith Tyo

    Article

    Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

    Insert

    Ntag-mCherry
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    pl-TetO

    Available since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

27. pTH848-gst-disec

    Plasmid

    #89589

    Purpose

    Plasmid for bacterial expression of His-tagged GST encoded by non-preferred codons

    Depositor

    Tobias von der Haar

    Article

    Jossé et al bioRxiv 200006

    Insert

    glutathione-S transferase codon-dis-optimised for bacterial expression
    

    Use

    Tags

    8xhistidine

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSaI14

    Plasmid

    #8614

    Depositor

    Robert Hartley

    Article

    Krajcikova et al J Bacteriol 1998 Mar;180(6):1582-5.

    Insert

    Ribonuclease inhibitor SaI14
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N terminal threonine changed to alanine

    Promoter

    Available since

    Jan. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

29. pCDF-EcAvs4

    Plasmid

    #188806

    Purpose

    Codon optimized EcAvs4 with C-terminal His tag

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

    Insert

    EcAvs4
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pZH512

    Plasmid

    #102666

    Purpose

    Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

    Depositor

    Zach Hensel

    Article

    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

    Insert

    GFPmut2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge