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We narrowed to 6,038 results for: WUS;

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pCMV-MtPylRS (human-opti)

Plasmid

#164080

Purpose

To express a Methanosarcina thermophila derived PylRS in mammalian cells for genetic code expansion

Depositor

Tao Liu

Article

Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2

Insert

MtPylRS-478(human-opti)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-MfBulKRS (human-opti)

Plasmid

#164196

Purpose

To express a Methanosarcina flavescens derived BulKRS in mammalian cells for genetic code expansion

Depositor

Tao Liu

Article

Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2

Insert

Mf BulKRS(human-opti)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Y269A;Y347F

Promoter

CMV

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pMXs-MB

Plasmid

#31703

Depositor

Duanqing Pei

Article

Liao et al J Biol Chem. 2011 May 13. 286(19):17359-

Insert

miR-106a-363

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV5 Flag Jnk1a1(TPF)

Plasmid

#15741

Depositor

Roger Davis

Article

Derijard et al Cell. 1994 Mar 25. 76(6):1025-37.

Insert

Jnk1a1(APY) (MAPK8 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Tyr-192Phe

Promoter

Available since

March 6, 2008

Availability

Academic Institutions and Nonprofits only
EF1a_KLF14_P2A_Hygro_Barcode

Plasmid

#120500

Purpose

Barcoded lentiviral vector to express KLF14 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF14 (KLF14 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pMXs-1002

Plasmid

#31711

Depositor

Duanqing Pei

Article

Liao et al J Biol Chem. 2011 May 13. 286(19):17359-

Insert

miR-302b, c, a, d

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pRS424-URR

Plasmid

#65330

Purpose

high copy number plasmid as receiving vector for exogenous pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

URR1-RFP-Leu2-URR2

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pMV-URR2

Plasmid

#65332

Purpose

standard plasmids for exogenus pathway assembly haboring recombination sequence 2 (Universal Recombination Region 2) which could be released and ligated to series of TUs

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

URR2

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pMV-URR1

Plasmid

#65331

Purpose

standard plasmids for exogenus pathway assembly haboring recombination sequence 1(Universal Recombination Region 1) which could be released and ligated to series of TUs

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

URR1

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pTorPE-GEX-GECO1

Plasmid

#32464

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

GEX-GECO1.0

Use

Lab constructed

Tags

6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…

Expression

Bacterial

Mutation

GCaMP3 K69E/K119I/L173Q/T223S/F315L/F368L/L372Q/K…

Promoter

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

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MTK0_012

Plasmid

#123967

Purpose

Encodes the hROSA26 BxBI recognition site with Hygromycin resistance and mRuby2 landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

hRosa26-BxBI landing pad

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 CARD8-TEV-GFP-FLAG S297A

Plasmid

#169992

Purpose

Mammalian purification construct for CARD8 with an autoprocessing-deficient mutation

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Tags

TEV-siriusGFP-FLAG

Expression

Mammalian

Mutation

S297A

Promoter

CMV

Available since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P41nmt1-GST

Plasmid

#39287

Depositor

John Pringle, Jurg Bahler

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

nmt1 promoter (nmt1 Fission Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Expression

Mutation

-1163 to +6 of nmt1 locus with a 4bp deletion (TA…

Promoter

Available since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only
pYPQ141-ZmUbi-RZ-Aa3.8.4

Plasmid

#136375

Purpose

Gateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 3.8 with MS2 at the third stem loop is used

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGL421_MLL2_promoter

Plasmid

#118696

Purpose

Express MLL2/KMT2B promoter for luciferase assay

Depositor

Christopher Vakoc

Article

Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

Insert

MLL_promoter

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pYPQ292 (AaCas12b)-D570A-TV

Plasmid

#136380

Purpose

CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AaCas12b (D570A)-TV

Use

CRISPR

Tags

Expression

Plant

Mutation

D570A

Promoter

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pInducer20 CARD8-HA PAM S297A

Plasmid

#169983

Purpose

Tet/Dox-inducible mammalian expression of CARD8 that is autoprocessing-deficient (S297A) with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

PAM, S297A

Promoter

Available since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_CDK6-P2A-Hygro_Barcode

Plasmid

#170224

Purpose

Barcoded lentiviral vector to express CDK6 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

CDK6 (CDK6 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pEYFP-N1-hRIP3-FL V460P

Plasmid

#61379

Purpose

Expresses Human full length RIPK3 with V460P mutation in the mammalian cells

Depositor

Francis Chan

Article

Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

Insert

Full length human RIP3 with V460Pmutation (RIPK3 Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

changed V460 to P

Promoter

Available since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only
pEYFP-N1-hRIP3-FL V458P

Plasmid

#61377

Purpose

Expresses Human full length RIPK3 with V458P mutation in the mammalian cells

Depositor

Francis Chan

Article

Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

Insert

Full length human RIP3 with V458Pmutation (RIPK3 Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

changed V458 to P

Promoter

Available since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only
JPF0522

Plasmid

#124013

Purpose

Encodes crippled pEF1a driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB_pEF1ac-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_HOXA1_P2A_Hygro_Barcode

Plasmid

#120451

Purpose

Barcoded lentiviral vector to express HOXA1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

HOXA1 (HOXA1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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JPF0454

Plasmid

#124039

Purpose

Encodes pPGK1 driving expression of spCas9 P2A mAzamiGreen in a 2nd gen. Lenti Virus destination vector

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pHR_pPGK1-spCAS9-P2A-mAzamiGreen

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221 NLRP1 M77T

Plasmid

#166818

Purpose

Gateway pDONR vector encoding NLRP1 with the patient mutation M77T (Zhong et al., 2016) (no stop codon)

Depositor

Daniel Bachovchin

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway shuttling vector

Tags

Expression

Mutation

M77T

Promoter

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 NLRP1 A54T

Plasmid

#166815

Purpose

Gateway pDONR vector encoding NLRP1 with the patient mutation A54T (Zhong et al., 2016) (no stop codon)

Depositor

Daniel Bachovchin

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway shuttling vector

Tags

Expression

Mutation

A54T

Promoter

Available since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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MTK8b_009

Plasmid

#123896

Purpose

Encodes the 3' 500 bp hThal5.10-2-hg18 locus homology arm as a type 8b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

LA_hThal5.10-2-hg18

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK234_027

Plasmid

#123907

Purpose

Encodes the spCas9 sgRNA1 hThal5.10-2_hg18 (site 1) gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

sgRNA1 -hThal5.10-2_hg18

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR221 NLRP1 FIIND (F1079M-L1373) S1213A

Plasmid

#166832

Purpose

Gateway pDONR vector containing NLRP1 FIIND with autoprocessing deficient S1213A mutation (no stop codon)

Depositor

Daniel Bachovchin

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway shuttling vector

Tags

Expression

Mutation

Promoter

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-dLbCpf1-BE0ΔiNLS

Plasmid

#107684

Purpose

Expresses pCMV-dLbCpf1-BE0ΔiNLS crRNA in mammalian cells

Depositor

Jia Chen

Article

Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

dLbCpf1-BE0ΔiNLS

Use

Tags

Expression

Mammalian

Mutation

D832A/E925A/D1148A

Promoter

CMV

Available since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

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MTK0_001

Plasmid

#123924

Purpose

Encodes the spCas9 gRNA GFP dropout expression cassette with ConLS and ConRE connectors with Ampicillin resistance as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

TU-sgRNA - L1/RE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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