We narrowed to 6,336 results for: WUS;

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pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

Plasmid

#42337

Purpose

Dual expression plasmid of human codon-optimized SpCas9 and a gRNA to the human Emx1 locus, can be used to test SpCas9 cleavage in cell lines of choice.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Available Since

Feb. 27, 2013

Availability

Academic Institutions and Nonprofits only

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RFP-PASS

Plasmid

#193971

Purpose

Monitoring the production of phosphatidic acid with RFP-PASS (phosphatidic acid biosensor with superior sensitivity)

Depositor

Guangwei Du

Article

Zhang et al Mol Cell Biol. 2014 Jan;34(1):84-95. doi

Insert

PA biosenor

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pRRL CAGpNFlag BAF155 ires GFP

Plasmid

#24561

Depositor

Jerry Crabtree

Article

Ho et al Proc Natl Acad Sci U S A. 2009 Mar 31. 1

Insert

BAF155 (Smarcc1 Mouse)

Use

Lentiviral

Tags

Flag

Available Since

July 16, 2010

Availability

Academic Institutions and Nonprofits only
pET41-MBP-t-A3A-t-His

Plasmid

#109231

Purpose

Bacterial expression of A3A with N-terminal MBP an C-terminal His tag, both cleavable with TEV protease

Depositor

Rahul Kohli

Article

Schutsky et al Nat Biotechnol. 2018 Oct 8. pii: nbt.420

Insert

human APOBEC3A (APOBEC3A Human, Optimized for E. coli expression)

Tags

His and MBP

Expression

Bacterial

Available Since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pMV-LEU2

Plasmid

#65334

Purpose

standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker LEU2 which could be released and ligated to series of TUs

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

LEU2

Use

Synthetic Biology

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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PCS2-FlipGFP(Casp3 cleavage seq)

Plasmid

#124432

Purpose

Expresses FlipGFP (caspase-3 cleavage sequence) protease reporter in zebrafish (verified)

Depositor

Xiaokun Shu

Article

Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

Insert

FlipGFP(Casp3 cleavage seq)

Use

Zebrafish (good expression)

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pSLIK-PUM1-rABE-Flag

Plasmid

#191385

Purpose

Doxycycline inducible expression of PUM1-rABE

Depositor

Stephen Floor

Article

Lin et al Nat Methods. 2023 Dec;20(12):1887-1899.

Insert

PUM1-rABE (PUM1 Human, Synthetic)

Use

Lentiviral

Tags

Flag

Promoter

tet-inducible promoter

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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EF1a_ESRRG_P2A_Hygro_Barcode

Plasmid

#120435

Purpose

Barcoded lentiviral vector to express ESRRG in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

ESRRG (ESRRG Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pY026

Plasmid

#84741

Purpose

Expresses huAsCpf1 and crRNA guide

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Insert

huAsCpf1

Tags

3xHA and NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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Lenti_SaCRISPR_GFP

Plasmid

#118636

Purpose

To clone sgRNA for SaCas9 (SauCas9)

Depositor

Christopher Vakoc

Article

Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

Type

Empty backbone

Use

Lentiviral

Promoter

U6

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pDA007

Plasmid

#131380

Purpose

ORFeus expression, WT

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

ORFeus-Hs

Tags

ORF2-3xFlag

Expression

Mammalian

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGIG_V5-PPP6C

Plasmid

#92013

Purpose

Expresses V5-PPP6C, includes IRES-Emerald

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

PPP6C (PPP6C Human)

Tags

V5

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pAcCas12nHS_RV6_PT1_empty

Plasmid

#203810

Purpose

Plasmid containing a CMV-driven NLS-AcCas12n-PT1-NLS(human-codonoptimized)cassette and a U6-driven sgRNA_V6 cassette(containing BsaI sites for spacer insertion). Carbenicillin resistance.

Depositor

Quanjiang Ji

Article

Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

Insert

AcCas12nHS_RV6_PT1

Use

CRISPR

Promoter

CMV

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pLEX307 ASC

Plasmid

#163977

Purpose

Mammalian expression vector encoding ASC (for example, to assess ASC-speck formation or inflammasome signalling assays)

Depositor

Daniel Bachovchin

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

ASC (PYCARD Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pINDUCER21-puro_TAOK3

Plasmid

#209778

Purpose

doxycycline-inducible expression of TAOK3

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

TAOK3 (TAOK3 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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L4440_BioBrick-hif-1-sgRNA-B

Plasmid

#177785

Purpose

Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hif-1 promoter)

Depositor

Michael Ristow

Article

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

hif-1 (hif-1 Nematode)

Expression

Bacterial

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ-dSpRY-Act3.0

Plasmid

#158416

Purpose

CRISPR-Act3.0 system containing dSpRYCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Insert

dSpRY-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter

Use

CRISPR

Expression

Plant

Available Since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pDA025

Plasmid

#131384

Purpose

ORFeus expression, mutant

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

ORFeus

Tags

ORF2-3xFlag

Expression

Mammalian

Mutation

H230A En

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-hA3B-BE3

Plasmid

#113411

Purpose

Expresses hA3B-BE3 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA3B-BE3 (APOBEC3B S. pyogenes and Bacteriophage PBS2, Human)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-NPC1(P202A/F203A)-FLAG

Plasmid

#164976

Purpose

Lentiviral vector for NPC1 expression in mammalian cells

Depositor

Roberto Zoncu

Article

Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

Insert

NPC1 (NPC1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

P202A/ F203A

Promoter

EF1a

Available Since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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pDA034

Plasmid

#131386

Purpose

ORFeus expression, mutant

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

ORFeus

Tags

ORF2-3xFlag

Expression

Mammalian

Mutation

D702Y RT

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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rAAV2/cMG.WPP

Plasmid

#184542

Purpose

AAV packaging plasmid, expressing Rep2 and AAV-cMG.WPP capsid

Depositor

Minmin Luo

Article

Lin et al Nat Methods. 2022 Jul 25. pii: 10.1038/s

Insert

AAV-cMG.WPP capsid

Use

AAV; Helper vector for packaging

Mutation

changes on AAV9 capsid: K449R; insertion of WPPKT…

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-ihSyn1-DIO-tTA

Plasmid

#99121

Purpose

An AAV genome with Cre-dependent expression of the tTA from the hSyn1 promoter. Contains a positive feedback loop for amplifed expression of the tTA

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Has Service

AAV1

Insert

DIO-tTA

Use

AAV

Expression

Mammalian

Mutation

*(see below)

Promoter

ihSyn1

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-dCpf1-BE

Plasmid

#107685

Purpose

Expresses dLbCpf1-BE in mammalian cells

Depositor

Jia Chen

Article

Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

dLbCpf1-BE

Expression

Mammalian

Mutation

D832A/E925A/D1148A

Promoter

CMV

Available Since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

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EF1a_GATA6_P2A_Hygro_Barcode

Plasmid

#120445

Purpose

Barcoded lentiviral vector to express GATA6 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

GATA6 (GATA6 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pY027

Plasmid

#84742

Purpose

Expresses huLbCpf1 and crRNA guide

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Insert

huLbCpf1

Use

CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pDA027

Plasmid

#131385

Purpose

ORFeus expression, mutant

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

ORFeus

Tags

ORF2-3xFlag

Expression

Mammalian

Mutation

H230A, D702Y

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pRB133

Plasmid

#180176

Purpose

GluN2A in pCDNA3.1

Depositor

Richard Bergeron

Article

Cappelli et al iScience. 2021 Dec 3;25(1):103539. doi:

Insert

GluN2A (Grin2a Rat)

Expression

Mammalian

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pDA056

Plasmid

#131387

Purpose

ORFeus expression, WT

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

ORFeus-Hs

Expression

Mammalian

Promoter

TRE3G

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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rAAV2/cMG.QRP

Plasmid

#184543

Purpose

AAV packaging plasmid, expressing Rep2 and AAV-cMG.QRP capsid

Depositor

Minmin Luo

Article

Lin et al Nat Methods. 2022 Jul 25. pii: 10.1038/s

Insert

AAV-cMG.QRP capsid

Use

AAV; Helper vector for packaging

Mutation

changes on AAV9 capsid: K449R; insertion of QRPPR…

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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