We narrowed to 12,703 results for: NUC

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pEGFP-C1-6xHis

Plasmid

#87343

Purpose

To express EGFP-6xHis in mammalian cell

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

6xHis

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCW57-mcGAS

Plasmid

#185458

Purpose

Inducible FLAG-tagged mouse cGAS

Depositor

Shitao Li

Article

Wu et al Commun Biol. 2022 May 10;5(1):433. doi:

Insert

cGAS

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pBEVY-GL

Plasmid

#51225

Purpose

bi-directional expression vector for yeast, galactose inducible, LEU2 selection

Depositor

Charles Miller

Article

Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

Type

Empty backbone

Expression

Yeast

Promoter

GAL1,10

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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coel::GFP

Plasmid

#8937

Depositor

Piali Sengupta

Article

Miyabayashi et al Dev Biol. 1999 Nov 15. 215(2):314-31.

Insert

unc-122 promoter (unc-122 Nematode)

Tags

GFP

Expression

Worm

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pCas1+2

Plasmid

#72676

Purpose

Express Cas1 and Cas2 for the CRISPR adaptation process in Escherichia coli. Has streptomycin resistance.

Depositor

Udi Qimron

Article

Yosef et al Nucleic Acids Res. 2012 Jul;40(12):5569-

Insert

Cas1, Cas2

Expression

Bacterial

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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pDestTol2-QUAS:GCaMP7a

Plasmid

#184817

Purpose

Used to generate the Tg(QUAS:GCaMP7a)c594 transgenic line. GCaMP7a from Muto et al., 2013 (https://doi.org/10.1016/j.cub.2012.12.040).

Depositor

Marnie Halpern

Article

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

QUAS:GCaMP7a-pA

Use

Zebrafish tol2 transgenesis

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAC-Kan-alphaGal4

Plasmid

#13421

Depositor

Keith Joung

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

RNAP alpha-Gal4

Expression

Bacterial

Available Since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

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pGWT35S-Citrine-NLS

Plasmid

#182583

Purpose

Transient expression of Citrine-NLS in plant cell (Nucleus)

Depositor

Yutaka Kodama

Article

Osaki et al PeerJ. 2017 Sep 7;5:e3779. doi: 10.7717/

Insert

NLS

Tags

Citrine

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pET-2(KW)2-eGFP

Plasmid

#112736

Purpose

Express 2(KW)2-eGFP in E. coli

Depositor

Kyubong Jo

Article

Lee et al Nucleic Acids Res. 2016 Jan 8;44(1):e6.

Insert

2(KW)2-eGFP

Tags

6xHis

Expression

Bacterial

Promoter

T7 Promoter

Available Since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCL1333

Plasmid

#217966

Purpose

Bacterial expression of RAN with an N-terminal 6His tag and a TEV cleavage site.

Depositor

Andrew Bowman

Article

Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755

Insert

RAN (RAN Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-survivin

Plasmid

#196908

Purpose

visualization of midzone on anaphase and midbody later during mitosis in living mammalian cells

Depositor

Kenji Sugimoto

Article

Fukada et al Biotechniques. 2004 Oct;37(4):552, 554,

Insert

survivin

Tags

EGFP

Expression

Mammalian

Mutation

E129K

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-ITSN2

Plasmid

#129615

Purpose

RhoGEF

Depositor

Dorus Gadella

Article

Reinhard et al Small GTPases. 2019 Aug 30:1-15. doi: 10

Insert

ITSN2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pBad33_ISAam1_reRNA

Plasmid

#205322

Purpose

Bacterial expression of ISAam1 TnpB and its reRNA

Depositor

Haoyi Wang

Article

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISAam1 TnpB

Expression

Bacterial

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-PRSX8ChR2 (H134R)eYFP

Plasmid

#89539

Purpose

Expresses ChR2 (H134R) -eYFP in Phox2 (including catecholamine) neurons

Depositor

Ruth Stornetta

Article

Holloway et al Eur J Neurosci. 2015 Sep;42(6):2271-82.

Insert

ChR2 (H134R)

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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AiRTX_ctrl

Plasmid

#214069

Purpose

Fluorescent reporter system, NMD negative control. Intron present in 5'UTR

Depositor

Sujatha Jagannathan

Article

Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

Insert

TurboRFP

Expression

Mammalian

Mutation

Remnant of linker sequence present at the end of …

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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LRT2B-GO2

Plasmid

#136909

Purpose

Lentivirus expressing GO2 sgRNA

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

sgGO2

Use

Lentiviral

Mutation

WT

Promoter

hU6

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry-RanV19

Plasmid

#118316

Purpose

Expression of human Ran

Depositor

Philip Stork

Article

Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

Insert

RanV19 (RAN Human)

Tags

mCherry

Expression

Mammalian

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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FRP467-PACT1(-1-520)-LexA-ER-haVP16

Plasmid

#58430

Purpose

Expresses LexA-ER-VP16 in yeast cells

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

LexA-ER-VP16

Expression

Yeast

Promoter

Act1 promoter (-520;1)

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pAd-PV-NLS-DsRed

Plasmid

#17287

Depositor

Anton Bennett

Article

Rodrigues et al J Biol Chem. 2007 Jun 8. 282(23):17061-8

Insert

PV-DsRed-NLS-CD (Pvalb Rat)

Use

Adenoviral

Tags

Myc

Expression

Mammalian

Available Since

Feb. 26, 2008

Availability

Academic Institutions and Nonprofits only
pET-2(KW)2-mCherry

Plasmid

#112738

Purpose

Express 2(KW)2-mCherry in E. coli

Depositor

Kyubong Jo

Article

Lee et al Nucleic Acids Res. 2016 Jan 8;44(1):e6.

Insert

2(KW)2-mCherry

Tags

6xHis

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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His_CL7_2NLS_iCas12a-4NLS

Plasmid

#222971

Purpose

Bacterial expression of NLS-rich iCas12a construct

Depositor

Jennifer Doudna

Article

Chen et al Nat Commun. 2024 Feb 26;15(1):1727. doi:

Insert

His_CL7_2NLS_iCas12a-4NLS

Use

CRISPR

Tags

CL7 and His

Expression

Bacterial

Promoter

tac

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTH650-RLuc/staFLuc

Plasmid

#29696

Depositor

Tobias von der Haar

Article

Chu et al Nucleic Acids Res. 2011 Aug;39(15):6705-

Insert

Firefly Luciferase

Expression

Yeast

Available Since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pAd-PV-NES-DsRed

Plasmid

#17286

Depositor

Anton Bennett

Article

Rodrigues et al J Biol Chem. 2007 Jun 8. 282(23):17061-8

Insert

PV-NES (Pvalb Rat)

Use

Adenoviral

Tags

DSR and Myc

Expression

Mammalian

Available Since

Feb. 26, 2008

Availability

Academic Institutions and Nonprofits only
pSL4164 (pEffector_IscB,ωRNA)

Plasmid

#210874

Purpose

Expresses GstIscB and native ωRNA from constitutive promoters

Depositor

Samuel H. Sternberg

Article

Meers et al Nature. 2023 Sep 27. doi: 10.1038/s41586

Insert

GstIscB

Expression

Bacterial

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pBRG2

Plasmid

#149474

Purpose

Broad-Host range expression vector, pBBR origin, RhaR-RhaS activator, PrhaBAD promoter, GFPmut3b, Kanamycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

RhaS-RhaR-GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PrhaBAD

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pJKR-H-mphR

Plasmid

#62559

Purpose

Macrolide biosensor. High copy plasmid that expresses GFP proportional to macrolide concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pJKR-H-araC

Plasmid

#62563

Purpose

Arabinose biosensor. High copy plasmid that expresses GFP proportional to arabinose concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pNLS-LEXY (pDN138)

Plasmid

#72657

Purpose

golden gate entry vector for mCherry-LEXY tagging of proteins (N-terminal cMyc(P1A) NLS)

Depositor

Barbara Di Ventura, Roland Eils

Article

Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

Type

Empty backbone

Use

Golden gate entry vector

Tags

NLS and mCherry-LEXY

Expression

Mammalian

Promoter

CMV-F

Available Since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only

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mCherry-FGD1

Plasmid

#129612

Purpose

RhoGEF

Depositor

Dorus Gadella

Article

Reinhard et al Small GTPases. 2019 Aug 30:1-15. doi: 10

Insert

FGD1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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GST-PRMT6

Plasmid

#34700

Depositor

Steven Clarke

Article

Frankel et al J Biol Chem. 2002 Feb 1;277(5):3537-43.

Insert

PMRT6

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only