We narrowed to 34,443 results for: CaS

1. pHR-UCOE-CMV-dCas9-XTEN80-KRAB(Kox1)-P2A-EGFP

   Plasmid

   #188772

   Purpose

   dCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

   Depositor

   Jonathan Weissman, Marco Jost

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   dCas9-XTEN80-KRAB(Kox1)
   

   Use

   Lentiviral

   Tags

   HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBBK95 pCAS-Tyr-[gRNA: 2xBsaI+NotI cut sites] (HygR)

   Plasmid

   #179079

   Purpose

   Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Hygromycin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSLQ11274:pHR-hU6-crTet-HSP-hyperdCas12a-miniVPR-CMV-mCherry

   Plasmid

   #210603

   Purpose

   heat shock promoter - activated hyperdCas12a-based transcriptional activator

   Depositor

   Stanley Qi

   Article

   Liu et al Nat Commun. 2023 Oct 18;14(1):6575. doi:

   Insert

   hyperdCas12a-miniVPR
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   truncated hsp70

   Available since

   Dec. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBZ186-pU6-(BbsI)_CBh-sv40NLS-Cas9-NLS T2A-mCherry

   Plasmid

   #170182

   Purpose

   Mammalian expression vector for sgRNAs cloned into the BbsI sites and for expression of spCas9 linked to mCherry via a T2A peptide

   Depositor

   Hunter Fraser

   Article

   Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris

   Insert

   mCherry
   

   Use

   Tags

   3XFlag and T2A

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHR-UCOE-EFS-dCas9-XTEN80-KRAB(Kox1)-P2A-EGFP

   Plasmid

   #188771

   Purpose

   dCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

   Depositor

   Jonathan Weissman, Marco Jost

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   dCas9-XTEN80-KRAB(Kox1)
   

   Use

   Lentiviral

   Tags

   HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

   Expression

   Mutation

   Promoter

   EFS

   Available since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBBK01 pCAS-Tyr-[gRNA: BsaI GFP dropout] (KanR, AmpR)

   Plasmid

   #178985

   Purpose

   Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Kanamycin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJSC129 - Bacterial expression plasmid for SpCas9-HF1, REC2 FRET variant

   Plasmid

   #101211

   Purpose

   Bacterial expression plasmid for SpCas9-HF1, REC2 FRET variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/E60C/D273C/N497A/R661A/Q695A/Q926A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, E60C, D273C, N497A, R661A, Q695A and…

   Promoter

   T7

   Available since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJSC057 - Bacterial expression plasmid for SpCas9∆REC3, REC2 FRET variant

   Plasmid

   #101204

   Purpose

   Bacterial expression plasmid for SpCas9∆REC3, REC2 FRET variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/E60C/D273C/M1–N497,GGS,V713–D1368
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, E60C, D273C, M1-N497, GGS, V713-D1368

   Promoter

   T7

   Available since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBBK05 pCAS-Tyr-[gRNA: BsaI GFP dropout] (NatR,AmpR)

   Plasmid

   #178989

   Purpose

   Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Nourseothricin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. JI501: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 gRNA scaffold

    Plasmid

    #121841

    Purpose

    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; empty hU6-driven SaCas9 gRNA scaffold for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to dCas9 w/ gRNA expression

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 874V=βTub-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

    Plasmid

    #159672

    Purpose

    Plasmid supports Cas9 expression only in males, Opie2-dsRed tagged, and can be integrated with pBac or phC31.

    Depositor

    Omar Akbari

    Article

    Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

    Insert

    βTub-NLS-hSpCas9-T2A-GFP
    

    Use

    Tags

    T2A-GFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. SIN-cPPT-H1-sgHTT1-U6-sgGFP2-7SK-sgCas9-PGK-mCherry-WPRE

    Plasmid

    #87919

    Purpose

    HIV-1 SIN lentiviral transfer vector

    Depositor

    Nicole Déglon

    Article

    Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

    Insert

    sgHTT1, shGFP2, sgCas9, cherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    H1, U6, 7SK, PGK

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBZ191-pU6-sgBFP-CBh-sv40NLS-Cas9-NLS-T2A-mCherry

    Plasmid

    #170183

    Purpose

    Expression vector for a sgRNA against BFP and spCas9 linked to mCherry via a T2A peptide

    Depositor

    Hunter Fraser

    Article

    Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris

    Insert

    sgBFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRP[2CRISPR]-mCherry/Hygro-hCas9-U6>{mdx left}-U6>{mdx right}

    Plasmid

    #184381

    Purpose

    This plasmid contains hCas9 and two gRNAs that can excise the genomic area around the mouse mdx mutation in dystrophin's exon 23

    Depositor

    Ori Bar-Nur

    Article

    Domenig et al Stem Cell Reports. 2022 Feb 8;17(2):321-

    Insert

    Cas9, two gRNAs targeting dystrophin
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 874U=ExuL-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

    Plasmid

    #159671

    Purpose

    Plasmid supports Cas9 expression only in males, Opie2-dsRed tagged, and can be integrated with pBac or phC31.

    Depositor

    Omar Akbari

    Article

    Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

    Insert

    ExuL-NLS-hSpCas9-T2A-GFP
    

    Use

    Tags

    T2A-GFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-T7-nSpCas9(H840A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES140)

    Plasmid

    #185490

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage nSpCas9(H840A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP

    Depositor

    Raul Perez-Jimenez, Benjamin Kleinstiver

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    human codon usage nSpCas9(H840A)-BPNLS-P2A-eGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-eGFP

    Expression

    Mammalian

    Mutation

    H840A

    Promoter

    CMV and T7

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMB1052:miniTol2-U6-(empty)SaCas9_gRNA-CAG-Cre-P2A-NeoR-WPRE

    Plasmid

    #168108

    Purpose

    Tol2 transposon vector of empty U6-S.aureus Cas9 gRNA cassette and CAG-driven Cre-NeoR

    Depositor

    Erich Jarvis

    Article

    Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 1095=Rcd-1r-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

    Plasmid

    #159673

    Purpose

    Plasmid supports Cas9 expression in both males and females, Opie2-dsRed tagged, and can be integrated with pBac or phC31.

    Depositor

    Omar Akbari

    Article

    Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

    Insert

    Rcd-1r-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40
    

    Use

    Tags

    T2A-GFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET-28b-T7-hAsCas12a(E174R/S542R)-NLS(nucleoplasmin)-6xHis (AAS1880)

    Plasmid

    #114071

    Purpose

    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a(E174R/S542R) with C-terminal NLS and His-tag

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized AsCas12a (E174R/S542R) with C-terminal NLS and His-tag
    

    Use

    Tags

    NLS(nucleoplasmin) and 6xHis

    Expression

    Bacterial

    Mutation

    E174R and S542R

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR GLIS3 TSS-guide3

    Plasmid

    #118175

    Purpose

    CRISPR-mediated repression of GLIS3. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR CRY2-TSS-guide1

    Plasmid

    #125428

    Purpose

    CRISPR-mediated repression of CRY2. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR CRY2-TSS-guide2

    Plasmid

    #125429

    Purpose

    CRISPR-mediated repression of CRY2. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR C2CD4B-TSS-guide2

    Plasmid

    #125421

    Purpose

    CRISPR-mediated repression of C2CD4B. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR C2CD4A-TSS-guide4

    Plasmid

    #125419

    Purpose

    CRISPR-mediated repression of C2CD4A. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR VPS13C-TSS-guide3

    Plasmid

    #125414

    Purpose

    CRISPR-mediated repression of VPS13C. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR C2CD4AB-enh2-guide3

    Plasmid

    #125406

    Purpose

    CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs7163757 (C2CD4A/B GWAS locus)

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR C2CD4AB-enh1-guide3

    Plasmid

    #125402

    Purpose

    CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs182222907 (C2CD4A/B GWAS locus)

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    July 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR C2CD4AB-enh3-guide3

    Plasmid

    #125410

    Purpose

    CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs17205526 (C2CD4A/B GWAS locus)

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pX601-AAV-CMV-NLS-SaCas9-NLS-3xHA-bGHpA;U6-Cre-1

    Plasmid

    #237890

    Purpose

    Cre-KO AAV vector#1 containing SaCas9 and its sgRNA targeting Cre

    Depositor

    Rui Lin

    Insert

    sgRNA-Cre
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pX601-AAV-CMV-NLS-SaCas9-NLS-3xHA-bGHpA;U6-Cre-2

    Plasmid

    #237891

    Purpose

    Cre-KO AAV vector#2 containing SaCas9 and its sgRNA targeting Cre

    Depositor

    Rui Lin

    Insert

    sgRNA-Cre
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pX601-AAV-CMV-NLS-SaCas9-NLS-3xHA-bGHpA;U6-Cre-3

    Plasmid

    #237892

    Purpose

    Cre-KO AAV vector#3 containing SaCas9 and its sgRNA targeting Cre

    Depositor

    Rui Lin

    Insert

    sgRNA-Cre
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCAG-eCas9-T2A-EGFP-U6-gRNA targeting Nrl-no ITR and f1 ori

    Plasmid

    #234737

    Purpose

    All-in-one CRISPR/Cas9 vector encodes high-fidelity eSpCas9 and a gRNA targeting Nrl, efficiently reprogramming rod precursors into cone-like cells in the mouse retina.

    Depositor

    Kirill Martemyanov

    Article

    Zhao et al Nat Commun. 2025 Apr 22;16(1):3768. doi:

    Insert

    Nrl (Nrl Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSLQ9713: pHR-PGK-SV40_NLS-dCasMINI-V4-QNZF-c-Myc_NLS-mCherry-WPRE

    Plasmid

    #203219

    Purpose

    Expression of dCasMINI-V4-QNZF-mCherry in mammalian cells

    Depositor

    Stanley Qi

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    dCasMINI-V4-QNZF-mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLV hU6-VEGFR2#3 sgRNA Nestin-dCas9-KRAB-T2a-GFP

    Plasmid

    #196990

    Purpose

    Derived from pLV hU6-sgRNA Nestin-dCas9-KRAB-T2a-GFP with KDR sgRNA

    Depositor

    Elizabeth Kirby

    Article

    Dause et al Mol Neurobiol. 2025 Mar 25. doi: 10.1007

    Insert

    VEGFR2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLV-RnSyt1-sgRNA2-dCas9-KRAB-TagBFP2 (identifier AAAA-0246)

    Plasmid

    #202555

    Purpose

    Synaptotagmin-1 sgRNA2 with TagBFP2

    Depositor

    Frederic Meunier

    Article

    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

    Insert

    CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCAGTACTCGCGTGCCTCGCACCGG) (Syt1 Rat)
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLV-RnSyt1-sgRNA3-dCas9-KRAB-TagBFP2 (Identifier AAAA-0247)

    Plasmid

    #202556

    Purpose

    Synaptotagmin-1 sgRNA3 with TagBFP2

    Depositor

    Frederic Meunier

    Article

    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

    Insert

    CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCTCCTCCTGCAGCGGCAGCATCGG) (Syt1 Rat)
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLV-RnSyt1-sgRNA1-dCas9-KRAB-TagBFP2 (identifier AAAA-0245)

    Plasmid

    #202554

    Purpose

    Synaptotagmin-1 sgRNA1 with TagBFP2

    Depositor

    Frederic Meunier

    Article

    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

    Insert

    CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ -CACCGCGTGCCTCGCACCGGTCCGCGG) (Syt1 R. norvegius (gRNA))
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pRP[CRISPR]-EGFP/Puro-hCas9-U6>(long left)-U6>(long right)

    Plasmid

    #216871

    Purpose

    This plasmid contains hCas9 and two gRNAs that can excise the genomic area around the mouse mdx mutation in dystrophin's exon 23

    Depositor

    Ori Bar-Nur

    Article

    Lenardic et al J Clin Invest. 2024 May 7:e166998. doi:

    Insert

    Cas9, gRNA Long.L, gRNA Long.R (Dmd Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    mdx mutation in mouse dystrophin

    Promoter

    Available since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pJT256 Lenti pEF-dCas9-3XFLAG-N+F-T2A-tagBFP-P2A-Blast

    Plasmid

    #187332

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    dCas9-3XFLAG-N+F-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pJT257 Lenti pEF-dCas9-3XFLAG-Z+F-T2A-tagBFP-P2A-Blast

    Plasmid

    #187333

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    dCas9-3XFLAG-Z+F+N-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pJT255 Lenti pEF-dCas9-3XFLAG-N+Z-T2A-tagBFP-P2A-Blast

    Plasmid

    #187331

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    dCas9-3XFLAG-N+Z-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pJT331 Lenti pEF-dCas9-3XFLAG-Q+N+Z+F-T2A-tagBFP-P2A-Blast

    Plasmid

    #195538

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Lenti pEF-dCas9-3XFLAG-Q+N+Z+F-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCMV-T7-nSpCas9(D10A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES24)

    Plasmid

    #185492

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage nSpCas9(D10A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP

    Depositor

    Raul Perez-Jimenez, Benjamin Kleinstiver

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    human codon usage nSpCas9(D10A)-BPNLS-P2A-eGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-eGFP

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    CMV and T7

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pBBK02 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitKanR, AmpR)

    Plasmid

    #178986

    Purpose

    Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitKanamycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pBBK10 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitURA3,AmpR)

    Plasmid

    #178994

    Purpose

    Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitURA3

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pBBK08 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitLEU2,AmpR)

    Plasmid

    #178992

    Purpose

    Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitLEU2

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pBBK06 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitNatR,AmpR)

    Plasmid

    #178990

    Purpose

    Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitNourseothrycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. LB001: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::Sp/xCas9 TRE#2 gRNA

    Plasmid

    #131757

    Purpose

    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven Sp/xCas9 TRE#2 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#2 gRNA expression

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    TRE#2 Sp/xCas9 gRNA
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Oct. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. LA901: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::Sp/xCas9 TRE#1 gRNA

    Plasmid

    #131756

    Purpose

    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven Sp/xCas9 TRE#1 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#1 gRNA expression

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    TRE#1 Sp/xCas9 gRNA
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Oct. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. JV201: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 TRE#1 gRNA

    Plasmid

    #131755

    Purpose

    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven SaCas9 TRE#1 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#1 gRNA expression

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    TRE#1 SaCas9 gRNA
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Oct. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge