We narrowed to 12,674 results for: sequence

1. pMBP-HRV3C-AcrIF9

   Plasmid

   #141442

   Purpose

   Expression of AcrIF9 in E. coli, tagged with an HRV3C-cleavage fusion to Maltose Binding Protein.

   Depositor

   Blake Wiedenheft

   Article

   Hirschi et al Nat Commun. 2020 Jun 1;11(1):2730. doi:

   Insert

   AcrIF9
   

   Use

   Tags

   HRV3C cleavage site and Maltose Binding Protein

   Expression

   Bacterial

   Mutation

   Promoter

   ptac

   Available since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAT-9251

   Plasmid

   #124226

   Purpose

   Bacterial SpCas9-HF1 expression

   Depositor

   Ervin Welker

   Article

   Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

   Insert

   SpCas9-HF1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   TetR/TetA

   Available since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pUPD2 linker-RDF (GB2892)

   Plasmid

   #160604

   Purpose

   RDF sequence for C-terminal fusion with PhiC31 recombinase, together with a 18-nt linker sequence

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

   Insert

   RDF
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAV-U6+27-Linear-2_PolyU(1)_HairpinDistance(10)nts_Tornado-Corn

   Plasmid

   #159501

   Purpose

   Tests for the impacts 1 uracil in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination Module:Linear-2_PolyU(1)_HairpinDistance(10)nts
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6-27

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAV-U6+27-Linear-2_PolyU(4)_HairpinDistance(10)nts_Tornado-Corn

   Plasmid

   #159502

   Purpose

   Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination Module:Linear-2_PolyU(4)_HairpinDistance(10)nts
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6-27

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAV-U6+27-Linear-3_PolyU(1)_HairpinDistance(10)nts_Tornado-Corn

   Plasmid

   #159504

   Purpose

   Tests for the impacts 1 uracil in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination Module:Linear-3_PolyU(1)_HairpinDistance(10)nts
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6-27

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAV-U6+27-Linear-3_PolyU(4)_HairpinDistance(10)nts_Tornado-Corn

   Plasmid

   #159506

   Purpose

   Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination Module:Linear-3_PolyU(4)_HairpinDistance(10)nts
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6-27

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAV-U6+27-Linear-3_PolyU(4)_HairpinDistance(0)nts_Tornado-Corn

   Plasmid

   #159508

   Purpose

   Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with an immediately upstream hairpin have on human polymerase III transcription elongation from a U6 promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination Module:Linear-3_PolyU(4)_HairpinDistance(0)nts
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6-27

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAV-U6+27-Linear-3_PolyU(4)_HairpinDistance(16)nts_Tornado-Corn

   Plasmid

   #159511

   Purpose

   Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 16 nts upstream have on human polymerase III transcription elongation from a U6 promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination Module:Linear-3_PolyU(4)_HairpinDistance(16)nts
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6-27

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAV-U6+27-Linear-3_PolyU(4)_HairpinDistance(19)nts_Tornado-Corn

    Plasmid

    #159514

    Purpose

    Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 19 nts upstream have on human polymerase III transcription elongation from a U6 promoter.

    Depositor

    Julius Lucks

    Article

    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

    Insert

    Termination Module:Linear-3_PolyU(4)_HairpinDistance(19)nts
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6-27

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAV-U6+27-Linear-3_PolyU(4)_HairpinDistance(20)nts_Tornado-Corn

    Plasmid

    #159515

    Purpose

    Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 20 nts upstream have on human polymerase III transcription elongation from a U6 promoter.

    Depositor

    Julius Lucks

    Article

    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

    Insert

    Termination Module:Linear-3_PolyU(4)_HairpinDistance(20)nts
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6-27

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAV-U6+27-Linear-2_PolyU(1)_HairpinDistance(0)nts_Tornado-Corn

    Plasmid

    #159499

    Purpose

    Tests for the impacts 1 uracil in a poly-uracil tract in conjunctiuon with an immediately upstream hairpin have on human polymerase III transcription elongation from a U6 promoter.

    Depositor

    Julius Lucks

    Article

    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

    Insert

    Termination Module:Linear-2_PolyU(1)_HairpinDistance(0)nts
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6-27

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAV-U6+27-Linear-1_PolyU(4)_HairpinDistance(10)nts_Tornado-Corn

    Plasmid

    #159498

    Purpose

    Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.

    Depositor

    Julius Lucks

    Article

    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

    Insert

    Termination Module:Linear-1_PolyU(4)_HairpinDistance(10)nts
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6-27

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAV-U6+27-Linear-1_PolyU(4) _HairpinDistance(0)_Tornado-Corn

    Plasmid

    #159496

    Purpose

    Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with an immediately upstream hairpin have on human polymerase III transcription elongation from a U6 promoter.

    Depositor

    Julius Lucks

    Article

    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

    Insert

    Termination Module:Linear-1_PolyU(4) _HairpinDistance(0)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6-27

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAV-U6+27-Linear-1_PolyU(1)_HairpinDistance(0)nts_Tornado-Corn

    Plasmid

    #159495

    Purpose

    Tests for the impacts 1 uracil in a poly-uracil tract in conjunctiuon with an immediately upstream hairpin have on human polymerase III transcription elongation from a U6 promoter.

    Depositor

    Julius Lucks

    Article

    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

    Insert

    Termination Module:Linear-1_PolyU(1)_HairpinDistance(0)nts
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6-27

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pME-mKate2-GPI-pA

    Plasmid

    #109542

    Purpose

    Multisite gateway vector for expression of mKate2 fused to the 5' and 3' sequences from zebrafish folate receptor 1

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    mKate2 fused to the 5' and 3' sequences from zebrafish folate receptor 1
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSTAP-seq_fly-zfh1_spikeIn-3

    Plasmid

    #86389

    Purpose

    Expression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoter

    Depositor

    Alexander Stark

    Article

    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

    Insert

    D. melanogaster zfh1 enhancer & D. pseudoobscura ush core promoter -TSS#1
    

    Use

    Stap-seq spike in vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSTAP-seq_fly-zfh1_spikeIn-4

    Plasmid

    #86390

    Purpose

    Expression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoter

    Depositor

    Alexander Stark

    Article

    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

    Insert

    D. melanogaster zfh1 enhancer & D. pseudoobscura ush core promoter -TSS#2
    

    Use

    Stap-seq spike in vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. sgRNA(MS2)_Prnp_SAM2

    Plasmid

    #78625

    Purpose

    Encodes sgRNA2.0 targeting 138nt upstream of TSS

    Depositor

    Walker Jackson

    Article

    Kaczmarczyk et al PLoS One. 2016 Apr 29;11(4):e0154604. do

    Insert

    sgRNA(MS2)_Prnp_SAM2 (Prnp Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. sgRNA(MS2)_Prnp_SAM1

    Plasmid

    #78624

    Purpose

    Encodes sgRNA2.0 targeting 158nt upstream of TSS

    Depositor

    Walker Jackson

    Article

    Kaczmarczyk et al PLoS One. 2016 Apr 29;11(4):e0154604. do

    Insert

    sgRNA(MS2)_Prnp_SAM2 (Prnp Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge