163,606 results

1. pCAGGs-NLS-TIR1_P2A_NES-TIR1

   Plasmid

   #117699

   Purpose

   Expression of NLS-(Os)TIR1-HA and Myc-NES-(Os)TIR1from one mRNA

   Depositor

   Joerg Mansfeld

   Article

   Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

   Inserts

   TRANSPORT INHIBITOR RESPONSE 1
   TRANSPORT INHIBITOR RESPONSE 1
   

   Use

   Tags

   HA tag, Myc-NES, and NLS

   Expression

   Mammalian

   Mutation

   codon optimized for murine expression

   Promoter

   None, 2A peptide from porcine teschovirus-1 polyp…

   Available Since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-TA-ERN

   Plasmid

   #80474

   Purpose

   All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (constitutive rtTA and neomycin resistance)

   Depositor

   Knut Woltjen

   Article

   Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

   Type

   Empty backbone

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMPRA1

   Plasmid

   #49349

   Purpose

   Basic backbone for transient transfection of Massively Parallel Reporter Assay (MPRA) libraries

   Depositor

   Tarjei Mikkelsen

   Article

   Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-sfGFP 150TAG

   Plasmid

   #85493

   Purpose

   pET28a expressing E. coli codon optimized superfolder GFP with TAG stop codon at position 150

   Depositor

   Ryan Mehl

   Article

   Peeler et al Methods Mol Biol. 2012;794:125-34. doi:

   Insert

   superfolder GFP 150TAG
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   150 TAG (amino acid 150 mutated to stop codon)

   Promoter

   T7

   Available Since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLIX403_control_APOBEC_HA_P2A_mRuby_Capture1

   Plasmid

   #183951

   Purpose

   Inducible lentiviral expression, TRE-control-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA

   Depositor

   Eugene Yeo

   Article

   Brannan et al Nat Methods. 2021 May;18(5):507-519. doi

   Insert

   APOBEC1 (Apobec1 Rat)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE promoter, Tet ON

   Available Since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. GW1-PercevalHR

   Plasmid

   #49082

   Purpose

   Mammalian expression vector (CMV promoter) of PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

   Depositor

   Gary Yellen

   Article

   Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

   Insert

   PercevalHR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lv EF1a dCas9-HA 2A Blast

   Plasmid

   #102812

   Purpose

   Lentiviral plasmid from FIRE-Cas9 system to express dCas9-HA fusion protein

   Depositor

   Jerry Crabtree

   Article

   Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

   Insert

   dCas9-HA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A and N863A (dCas9)

   Promoter

   EF1a

   Available Since

   Jan. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. Arl13b-EGFP

   Plasmid

   #234844

   Purpose

   A mammalian expression plasmid encoding Arl13b with EGFP at C-terminus.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   Arl13b (ARL13B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. HaloTag-bActinCDS-bActinUTR-MS2V5

   Plasmid

   #102718

   Purpose

   Halo-Actin reporter for detection of both mRNA and protein

   Depositor

   Robert Singer

   Article

   Yoon et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

   Insert

   HaloTag-bActinCDS-bActin3'UTR-MS2V5
   

   Use

   Lentiviral

   Tags

   HaloTag and MS2V5

   Expression

   Mutation

   Promoter

   UbC

   Available Since

   Nov. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5/FRT/TO_H2B-HaloTag7-T2A-Tom20-HaloTag9

    Plasmid

    #175547

    Purpose

    Co-expression of HaloTag7 (nuclear) and 9 (mitochondrial surface) in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    H2B-HaloTag7 and Tomm20-HaloTag9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC18-mini-Tn7T-Gm-lux

    Plasmid

    #64963

    Purpose

    mini-Tn7 luxCDABE transcriptional fusion vector

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Insert

    lux operon
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pmStayGold_mStayGold_I3

    Plasmid

    #239339

    Purpose

    Expresses the I3-01 nanocage subunit N-terminally tagged with two copies of mStayGold in mammalian cells. Assembles into nanocages tagged with 120 FPs.

    Depositor

    Torsten Wittmann

    Article

    Viola et al J Cell Sci. 2025 May 15;138(10):jcs26385

    Insert

    I3-01
    

    Use

    Tags

    mStayGold

    Expression

    Mammalian

    Mutation

    K129A

    Promoter

    CMV

    Available Since

    June 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX330-CANX

    Plasmid

    #227279

    Purpose

    Expresses SpCas9 and a sgRNA targeting the C-terminus of CANX for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of CANX (CANX Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTubulin-Electra2

    Plasmid

    #184930

    Purpose

    Tubulin labeling with blue fluorescent protein Electra2

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    TUBA1B-Electra2
    

    Use

    Tags

    Electra2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSL4393 (pClpX_Eco)

    Plasmid

    #200907

    Purpose

    Expresses human codon-optimized E. coli ClpX from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    EcoClpX
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCCl-MCS_miniCMV_EGFP

    Plasmid

    #134984

    Purpose

    The plasmid includes a EGFP gene driven by a minimal CMV promoter. Cis-regulatory elements can be cloned into the single EcoRV site. This plasmid can be used for generating a lentiviral vector

    Depositor

    Claudio Mussolino

    Article

    Roman Azcona et al Nat Protoc. 2020 Oct;15(10):3410-3440. d

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    minCMV

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP SMG1 WT

    Plasmid

    #199571

    Purpose

    Expresses WT SMG1 fused to GFP

    Depositor

    Michael Kastan

    Article

    McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

    Insert

    SMG1 WT (SMG1 Human)
    

    Use

    Tags

    GFP fusion protein

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pVT100U-mtGFP

    Plasmid

    #45054

    Depositor

    Benedikt Westermann

    Article

    Westermann et al Yeast. 2000 Nov;16(15):1421-7.

    Insert

    mtGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH

    Available Since

    May 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAVS1-Tet-OsTIR1(WT)-V5

    Plasmid

    #158663

    Purpose

    Tet-OsTIR1(WT)-V5 donor for integration at the AAVS1 locus

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    Tet-OsTIR1(WT) AAVS1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. SNX9-pmCherryC1

    Plasmid

    #27678

    Depositor

    Christien Merrifield

    Article

    Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

    Insert

    SNX9 (Snx9 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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21. PB-U6insert-EF1puro

    Plasmid

    #104537

    Purpose

    A piggybac vector with puromycin resistance and a U6 promoter that allows cloning of guide RNAs

    Depositor

    George Church

    Article

    Kalhor et al Science. 2018 Aug 9. pii: science.aat980

    Type

    Empty backbone

    Use

    CRISPR, Mouse Targeting, and Synthetic Biology ; …

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. qTAG-C-Solo-mStayGold-CANX

    Plasmid

    #227280

    Purpose

    Donor template for mStayGold insertion into the C-terminus of the CANX locus. For ER visualization. To be co-transfected with sgRNA plasmid px330-CANX (Addgene #227279)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    CANX Homology Arms flanking a mStayGold Tag (CANX Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. pGL4.10-Claudin-1

    Plasmid

    #46387

    Purpose

    Claudin-1 promoter reporter assay

    Depositor

    David Mu

    Article

    Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-8

    Insert

    CLDN1 Promoter (CLDN1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    CLDN1 promoter

    Available Since

    Aug. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

24. p201N Cas9

    Plasmid

    #59175

    Purpose

    Cas9 driven by double 35S, nptII for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

    Depositor

    Wayne Parrott

    Article

    Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

    Inserts

    Cas9
    nptII
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    2x35S and StUbi-3P

    Available Since

    Sept. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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25. pCDNA5FRT/TO_HA-mAID-nanobody

    Plasmid

    #117713

    Purpose

    Expression of tetracycline-inducible HA-tagged lysine-less mAID fused to lysine-less vhhGFP4 (mAID-nanobody)

    Depositor

    Joerg Mansfeld

    Article

    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

    Insert

    mAID-nanobody
    

    Use

    Tags

    triple HA

    Expression

    Mammalian

    Mutation

    codon optimized mAID for murine expression

    Promoter

    CMV

    Available Since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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26. pSL3713 (pTnsC_Pse)

    Plasmid

    #200900

    Purpose

    Expresses human codon-optimized Pseudoalteromonas TnsC from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    PseTnsC
    

    Use

    Tags

    3xNLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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27. pMD2.G-VSV-G-mut

    Plasmid

    #196508

    Purpose

    VSV-G mutant (K47Q, R354A)

    Depositor

    Howard Chang

    Article

    Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

    Insert

    VSV-G
    

    Use

    Tags

    NA

    Expression

    Mammalian

    Mutation

    K47Q, R354A

    Promoter

    CMV

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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28. pSL1145 (pSPIN, pBBR1 backbone, R*MmeI)

    Plasmid

    #160736

    Purpose

    Single-plasmid V. cholerae CAST, encodes all proteins, crRNA, and donor DNA. Non-targeting crRNA with BsaI sites for spacer cloning. Mini-tn has MmeI site in R end for Tn-seq. pBBR1 backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST proteins, crRNA, and donor DNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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29. pDV05.2_pEF1a-iRFP720_secreted

    Plasmid

    #200409

    Purpose

    Expresses a secreted version of iRFP720 constitutively in mammalian cells

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    iRFP720 (secreted)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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30. BcLOV4-mammalian-recoded_mCherry_pcDNA3.1

    Plasmid

    #114595

    Purpose

    Encodes mCherry-tagged BcLOV4 for expression in mammalian cells

    Depositor

    Brian Chow

    Article

    Glantz et al Proc Natl Acad Sci U S A. 2018 Aug 14;11

    Insert

    BcLOV4
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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