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We narrowed to 4,675 results for: cre encoding plasmid

Showing: 2041 - 2060 of 4675 results

  1. JV201: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 TRE#1 gRNA

    Plasmid
    #131755
    Purpose
    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven SaCas9 TRE#1 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#1 gRNA expression
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    TRE#1 SaCas9 gRNA
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    hU6
    Available since
    Oct. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    131755-plasmid-map-sequence-id-314448

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  2. LB101: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 TRE#2 gRNA

    Plasmid
    #131758
    Purpose
    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven SaCas9 TRE#2 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#2 gRNA expression
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    TRE#2 SaCas9 gRNA
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    hU6
    Available since
    Oct. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    131758-plasmid-map-sequence-id-314395

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  3. KA901: pMVP (L3-L2) HA tag + pA; EF1a::eGFP-P2A-TETa-pA

    Plasmid
    #121804
    Purpose
    pMVP L3-L2 entry plasmid, contains HA tag-polyA + EF1a-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream EF1a-driven GFP-P2A-TETa
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    HA epitope tag-polyA + EF1a::eGFP-P2A-TETa-polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121804-plasmid-map-sequence-id-237313

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  4. KA701: pMVP (L3-L2) HA tag + pA; RIP::eGFP-P2A-TETa-pA

    Plasmid
    #121808
    Purpose
    pMVP L3-L2 entry plasmid, contains HA tag-polyA + RIP-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream RIP-driven GFP-P2A-TETa
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    HA epitope tag-polyA + RIP::eGFP-P2A-TETa-polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121808-plasmid-map-sequence-id-237328

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  5. pGL4.23 C5_15

    Plasmid
    #60322
    Purpose
    This plasmid contains a genomic fragment that is not active in human pancreatic islets, a minimal promoter and a luc2 gene. Can be used as negative control in reporter assays performed in human pancreatic islets.
    Depositor
    Jorge Ferrer
    Article
    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1
    Insert
    Non active element (nearest TSS PHLDA1) (PHLDA1 Human)
    Use
    Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 2, 2014
    Availability
    Academic Institutions and Nonprofits only

  8. MKC131_CCR5(SFFV-synEPOR-2A-YFP)

    Plasmid
    #232412
    Purpose
    AAV production plasmid for SFFV(synEPOR) vector from Figs. 2-3 that mediates HDR at CCR5 locus using CCR5 gRNA. YFP is followed by BGH polyA. AAV genome is flanked by AAV2 ITRs.
    Depositor
    Kyle Cromer
    Article
    Shah et al bioRxiv 2024.04.12.589260
    Insert
    Truncated Erythropoietin Receptor (EPOR Human)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    232412-plasmid-map-sequence-id-465038

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  9. MKC175_HBA1reg(synEPOR)

    Plasmid
    #232413
    Purpose
    AAV production plasmid for HBA1 UTRs vector from Fig. 3 that mediates HDR at HBA1 locus using HBA1 sg5 gRNA. HBB gene includes introns; HBA1 UTRs flank H+C15BA1 cassette. HAs are ~400bp each.
    Depositor
    Kyle Cromer
    Article
    Shah et al bioRxiv 2024.04.12.589260
    Insert
    Truncated Erythropoietin Receptor (EPOR Human)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    232413-plasmid-map-sequence-id-466145

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  13. pBOB-CAG-SARS-CoV2-Spike-HA

    Plasmid
    #141347
    Purpose
    3. Generation lentiviral vector expressing the codon optimized SARS-CoV2 Spike Glycoprotein ORF with a C-terminal HA tag generated by Junko Ogawa & Gerald M Pao
    Depositor
    Gerald Pao
    Article
    Ogawa et al bioRxiv. 2020 Jul 22. doi: 10.1101/2020.
    Insert
    SARS-CoV2 Spike Glycoprotein (S SARS-CoV2 hCoV19_USA EPI_ISL_414366 (GISAID))
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mutation
    resynthesized with human codon optimization nucle…
    Promoter
    CAG
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    141347-plasmid-map-sequence-id-296391

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  14. pBOB-CAG-SARS-CoV2-Spike D614G-HA

    Plasmid
    #158761
    Purpose
    3rd Generation lentiviral vector expressing the codon optimized SARS-CoV2 Spike Glycoprotein D614G high infectivity mutant with a C-terminal HA tag generated by Junko Ogawa & Gerald M Pao
    Depositor
    Gerald Pao
    Article
    Ogawa et al bioRxiv. 2020 Jul 22. doi: 10.1101/2020.
    Insert
    SARS-CoV2 Spike Glycoprotein (S SARS-CoV2 hCoV19_USA EPI_ISL_414366 (GISAID))
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Spike D614G
    Promoter
    CAG
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    158761-plasmid-map-sequence-id-309514

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  18. pU6-(BbsI)_CBh-Cas9-T2A-mCherry-H1-(BamHI)

    Plasmid
    #64217
    Purpose
    Expression vector for sgRNAs cloned into the BbsI sites, shRNAs into BamHI & AflII and for Expression of Cas9 linked to mCherry via T2A
    Depositor
    Ralf Kuehn
    Article
    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103
    Inserts
    Cas9
    hU6 promoter; BbsI sites for sgRNA
    H1 promoter; BamHI site for shRNA
    Use
    CRISPR
    Tags
    3xFLAG, NLS, and T2A-mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CBh, H1, and U6
    Available since
    Sept. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    64217-plasmid-map-sequence-id-123057

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  20. pECT3

    Plasmid
    #75450
    Purpose
    Plasmid that serves as the PCR template to create genomic pdt3 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate
    Depositor
    James Collins
    Article
    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.
    Insert
    pdt3
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    none
    Available since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75450-plasmid-map-sequence-id-146478

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Showing: 2041 - 2060 of 4675 results