12,610 results

1. pMK281 (mCherry2-Hygro)

   Plasmid

   #72797

   Purpose

   C-terminal tagging with mCherry2 using CRIPSR/Cas9

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   mCherry2-Hygro
   

   Use

   CRISPR

   Tags

   mCherry2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSicoR p53

   Plasmid

   #12090

   Depositor

   Tyler Jacks

   Article

   Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

   Insert

   p53 shRNA (Trp53 Mouse)
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   EGFP is expressed from this plasmid as a marker, …

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmCherry-Cep290

   Plasmid

   #27380

   Depositor

   Joseph Gleeson

   Article

   Valente et al Nat Genet. 2006 Jun . 38(6):623-5.

   Insert

   Cep290 (Cep290 Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   please see depositor comments below

   Promoter

   Available since

   June 1, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. mEGFP-PI35P2

   Plasmid

   #92419

   Purpose

   Fluorescent reporter for phosphatidylinositol (3,5)-bisphosphate (PI(3,5)P2). Mouse MCOLN1 residues 1–68 fused to eGFP.

   Depositor

   Geert van den Bogaart

   Article

   Baranov et al Small GTPases. 2017 May 10:0. doi: 10.10

   Insert

   MCOLN1 (Mcoln1 Mouse)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Synthetic gene, codon optimized for human.

   Promoter

   CMV

   Available since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHes1(467 RBPj(-))-luc

   Plasmid

   #43805

   Depositor

   Ryoichiro Kageyama

   Article

   Nishimura et al Genomics. 1998 Apr 1;49(1):69-75.

   Insert

   Hes1 Promoter (-467 to +46) RBPj(-) (Hes1 Mouse)
   

   Use

   Luciferase

   Tags

   Firefly luciferase

   Expression

   Mammalian

   Mutation

   Contains the murine Hes1 promoter (-467 to +46), …

   Promoter

   Hes1 promoter fragment (-467 to +46) (RBPj-)

   Available since

   April 5, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pST44-AP-1 core

   Plasmid

   #184809

   Purpose

   Bacterial expression of clathrin adaptor AP-1 core complex

   Depositor

   James Hurley

   Article

   Ren et al Cell. 2013 Feb 14;152(4):755-67. doi: 10

   Insert

   Hs AP1S3; Hs AP1B1 (1-584); Mm AP1G1 (1-595); Mm AP1M1
   

   Use

   Tags

   GST tag and His6

   Expression

   Bacterial

   Mutation

   AP1S3: S148C; AP1B1: K359R, E476K

   Promoter

   T7

   Available since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR-JNKKTRmRuby2

   Plasmid

   #59148

   Purpose

   ORF encoding JNK KTR mRuby2 flanked by Gateway sequences

   Depositor

   Markus Covert

   Article

   Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

   Insert

   JNK Kinase Translocation Reporter (MAPK8 Human, Mouse)
   

   Use

   Tags

   mRuby2

   Expression

   Mutation

   Promoter

   Available since

   Sept. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3/mArf1(T31N)-HA

   Plasmid

   #79411

   Purpose

   Expresses C-terminally HA-tagged Arf1(T31N) in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Kondo et al Cell Struct Funct. 2012;37(2):141-54. Ep

   Insert

   Arf1 (Arf1 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   changed Threonine 31 to Asparagine

   Promoter

   Available since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-pCALM1-sFLEx-HA-SpCas9-miniU6-sgRNAShank3

   Plasmid

   #213969

   Purpose

   AAV vector for encoding SpCas9 driven by pCALM1 promoter targeting Shank3 locus in the presence of Cre recombinase

   Depositor

   Zhonghua Lu

   Article

   Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

   Insert

   Shank3 sgRNA
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB-TAC-OKMS

    Plasmid

    #80480

    Purpose

    piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry)

    Depositor

    Knut Woltjen

    Article

    Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

    Insert

    OKMS (Pou5f1, Klf4, Myc, Sox2 Mouse)
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Klf4 [10-483]

    Promoter

    tetO

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CMV-FLEX-SaCas9-U6-sgOprk1

    Plasmid

    #159903

    Purpose

    Mutagenesis of Oprk1

    Depositor

    Larry Zweifel

    Insert

    Oprk1 (Oprk1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHR SFFVp BFP-Erk2

    Plasmid

    #50848

    Purpose

    Fluorescent reporter for Erk nuclear translocation

    Depositor

    Wendell Lim

    Article

    Toettcher et al Cell. 2013 Dec 5;155(6):1422-34. doi: 10

    Insert

    Erk2 (Mapk1 Mouse)
    

    Use

    Lentiviral

    Tags

    TagBFP

    Expression

    Mutation

    Promoter

    Available since

    Jan. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV-hSyn-Ribo-jGCaMP8s

    Plasmid

    #167572

    Purpose

    DEPRECATED: Please see Addgene item #169247 for an improved version of this plasmid. AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8s.

    Depositor

    Marianne Fyhn

    Article

    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

    Has Service

    AAV1

    Insert

    Ribo-GCaMP8s
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-Sst12-GFP

    Plasmid

    #172311

    Purpose

    SST interneuron-restricted gene regulatory element GRE12, to drive GFP expression in SST+ interneurons

    Depositor

    Michael Greenberg

    Article

    Hrvatin et al Elife. 2019 Sep 23;8. pii: 48089. doi: 1

    Insert

    Sst12
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    pB-Globin

    Available since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. Anti-HCN1 ion channel [N70/28R-1]

    Plasmid

    #206718

    Purpose

    Mammalian Expression Plasmid of anti-HCN1 ion channel (Rat). Derived from hybridoma N70/28-1.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-HCN1 ion channel (Rattus norvegicus) recombinant Mouse monoclonal antibody (Hcn1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Oct. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pmCherry-Paxillin

    Plasmid

    #80029

    Purpose

    Mammalian expression of mouse Paxillin

    Depositor

    Chinten James Lim

    Insert

    Paxillin (Pxn Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEF5B-FRT-AP-Smo-YFP-DEST

    Plasmid

    #49099

    Purpose

    Expressing AP-Smo-YFP in mammalian cells, can be used for establishing Flp-In stable cell lines

    Depositor

    Maxence Nachury

    Article

    Ye et al Elife. 2013 Aug 6;2:e00654. doi: 10.7554

    Insert

    Smo (Smo Mouse)
    

    Use

    Tags

    YFP and acceptor peptide (AP)

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Nov. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-EF1a-double floxed-Gad65-WPRE-HGHpA

    Plasmid

    #184631

    Purpose

    Expresses Gad65, driven by the Ef1a promoter, in a Cre-dependent fashion

    Depositor

    Nicolas Tritsch

    Article

    Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

    Insert

    Gad65 (Gad2 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-S5E2-C1V1-eYFP

    Plasmid

    #135633

    Purpose

    AAV vector to drive the expression of C1V1-eYFP in PV cortical interneuronsunder the control of the E2 regulatory element

    Depositor

    Jordane Dimidschstein

    Article

    Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

    Has Service

    AAV PHP.eB, AAV1, AAV5, and AAV9

    Insert

    C1V1-eYFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Aug. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hSyn-GRAB_g5-HT2h

    Plasmid

    #208721

    Purpose

    Expresses the green 5-HT sensor GRAB_g5-HT2h in neurons

    Depositor

    Yulong Li

    Article

    Deng et al bioRxiv 2023.05.27.542566

    Insert

    Green fluorescent 5-HT sensor GRAB_g5-HT2h
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge