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pZac2.1 gfaABC1D-Ezr-BioID2-HA

Plasmid

#176744

Purpose

Expresses Ezrin fused with BioID2 at the C-terminus in astrocytes

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

Ezr-BioID2-BioID2-HA

Use

AAV

Tags

HA

Expression

Bacterial

Promoter

gfaABC1D

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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8061-E31

Plasmid

#234268

Purpose

Gateway ORF Entry clone of mouse Smad1 with stop codon (for native or N-terminal fusions)

Depositor

Dominic Esposito

Insert

Smad1 (Smad1 Mouse)

Use

Gateway entry clone

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pScalps_Puro_mTet2 catalytic domain

Plasmid

#79554

Purpose

Expression of catalytic domain of mouse Tet2

Depositor

Silvia Monticelli

Article

Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

Insert

Tet2 (Tet2 Mouse)

Use

Lentiviral

Tags

Myc

Mutation

aa990-1912

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only
pB80-KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)_P2A_iLID-mCherry-RAB11

Plasmid

#174644

Purpose

Optogenetic coupling of RAB11 to opto-kinesin to induce anterograde transport of recycling endosomes

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)-P2A-iLID-mCherry-RAB11

Tags

Venus-SSPB and iLID-mCherry

Expression

Mammalian

Mutation

mmKIF1A(aa1-365): Pro202Ala; mVenus: Met1Del, Thr…

Promoter

Chicken beta-actin

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1-FAK-HA

Plasmid

#35039

Depositor

Anna Huttenlocher

Article

Chan et al J Biol Chem. 2010 Apr 9;285(15):11418-26

Insert

focal adhesion kinase (Ptk2 Mouse)

Tags

2x HA and mCherry

Expression

Mammalian

Mutation

Y42H; deletion of amino acids 1051 & 1052

Available Since

March 8, 2012

Availability

Academic Institutions and Nonprofits only

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pNJP

Plasmid

#115494

Purpose

Expression vector for NLS-iRFP-NLS, JNK KTR-mCherry, and mKO-MK2 in mammalian cells

Depositor

Kazuhiro Aoki

Article

Miura et al Cell Rep. 2018 Sep 4;24(10):2658-2668. d

Insert

NLS-iRFP-NLS-P2A-JNK KTR-mCherry-P2A-mKO-MK2 (MAPK14, MAPK8 Synthetic, Mouse, Human)

Tags

iRFP, mCherry, and mKO

Expression

Mammalian

Mutation

mCherry S227F, mCherry G229R, mKO V211G

Promoter

CAG

Available Since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-Axon-jGCaMP8m

Plasmid

#172719

Purpose

AAV transfer plasmid for neuronal expression of axon-targeted jGCaMP8m.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

Axon-jGCaMP8m

Use

AAV

Tags

6xHis

Expression

Mammalian

Promoter

hSyn

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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mEmerald-IFT88-N-18

Plasmid

#54125

Purpose

Localization: Cilium, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

IFT88 (Ift88 Mouse)

Tags

mEmerald

Expression

Mammalian

Mutation

N241D in IFT88

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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EGFP-Caveolin-3

Plasmid

#68396

Purpose

Fluorescently tagged Caveolin-3 for expression in mammalian cells

Depositor

Rob Parton

Article

Luetterforst et al J Cell Biol. 1999 Jun 28;145(7):1443-59.

Insert

Cav3 (Cav3 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only
pAAV-FLEX-SaCas9-U6-sgGrin2b

Plasmid

#124851

Purpose

Mutagenesis of Grin2b

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Grin2b (Grin2b Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pFUSEss-CHIg-mG2a_M18

Plasmid

#105930

Purpose

Expression plasmid coding for heavy chain of mouse IgG2a antibody specific to antigen B of the ABO blood group system, clone M18.

Depositor

Joanna Bereta

Article

Klaus et al Front Immunol. 2018 May 23;9:1096. doi:

Insert

IgG2b heavy chain (Ighg2b Mouse)

Expression

Mammalian

Promoter

hEF1-HTLV prom

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCW57-mcGAS

Plasmid

#185458

Purpose

Inducible FLAG-tagged mouse cGAS

Depositor

Shitao Li

Article

Wu et al Commun Biol. 2022 May 10;5(1):433. doi:

Insert

cGAS

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS-5HA-mISG15

Plasmid

#12444

Depositor

Dong-Er Zhang

Article

Kim et al Mol Cell Biol. 2004 Nov . 24(21):9592-60

Insert

ISG15

Tags

5xHA

Expression

Mammalian

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only
Cofilin-pmCherryC1

Plasmid

#27687

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

Cofilin (Cfl1 Mouse)

Tags

mCherry

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only

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GAD67 scFv [L127/8]

Plasmid

#190509

Purpose

Mammalian Expression of GAD67 scFV. Derived from hybridoma L127/8.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

GAD67 (Homo sapiens) recombinant scFV (GAD1 Mouse)

Tags

Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-iNOS

Plasmid

#19295

Depositor

Charles Lowenstein

Article

Lowenstein et al Proc Natl Acad Sci U S A. 1992 Aug 1. 89

Insert

inducible nitric oxide synthase (Nos2 Mouse)

Expression

Bacterial

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only

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mTLR2

Plasmid

#13083

Depositor

Ruslan Medzhitov

Insert

TLR2 (Tlr2 Mouse)

Expression

Mammalian

Available Since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only

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etnppl-pGEMtEasy

Plasmid

#192263

Purpose

Mouse Etnppl sequence is inserted in pGEM-T Easy vector.

Depositor

Toshihide Yamashita

Article

Tsujioka et al Front Cell Neurosci. 2023 Jan 30;17:1097

Insert

ethanolamine phosphate phospholyase (Etnppl Mouse)

Expression

Bacterial

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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PIG-Myc

Plasmid

#177650

Purpose

Expression of wild-type mouse Myc

Depositor

Trudy Oliver

Article

Mollaoglu et al Cancer Cell. 2017 Feb 13;31(2):270-285.

Insert

Myc (Myc Mouse)

Use

Retroviral

Tags

GFP and IRES

Expression

Mammalian

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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mouse Drp1(K38A)

Plasmid

#26049

Depositor

David Chan

Article

Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

Insert

Drp1 (Dnm1l Mouse)

Expression

Mammalian

Mutation

K38A GTPase mutant

Available Since

Sept. 20, 2010

Availability

Academic Institutions and Nonprofits only