We narrowed to 29,757 results for: REP

1. P[acman]-Omega2

   Plasmid

   #165861

   Purpose

   Omega2 Basic cloning vector GB2.0 compatible P[acman]-derived backbone. Allows for copy induction from low to high using AutoFOS or similar. Requires EPI300 bacterial strain (DH10B Derived) or similar. uses blue-white screening

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-tTALE-EmGFP-15b

   Plasmid

   #138280

   Purpose

   Express tTALE-EmGFP

   Depositor

   Kyubong Jo

   Article

   Shin et al Sci Rep. 2019 Nov 20;9(1):17197. doi: 10

   Insert

   7-mer tTALE-EmGFP
   

   Use

   TALEN

   Tags

   6x His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   April 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. MSCV Puro-EFS:GFP

   Plasmid

   #68484

   Purpose

   Retrovirus expressing GFP driven by EFS promoter

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Inserts

   Puromycin Resistance
   GFP
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS and LTR

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT2-cryR;tmem88aCE1-P1Egfp

   Plasmid

   #90148

   Purpose

   contains tmem88a enhancer sequence, and tmem88a specific gene promoter driving expression of eGFP

   Depositor

   Nathan Lawson

   Article

   Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

   Insert

   tmem88aE1P1Egfp (tmem88a Zebrafish)
   

   Use

   Tags

   EGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. MAC_C_INSR

   Plasmid

   #187788

   Purpose

   MAC-tagged gene expression of human INSR

   Depositor

   Markku Varjosalo

   Article

   Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

   Insert

   INSR (INSR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. TOPO CCSP

   Plasmid

   #68453

   Purpose

   TOPO construct expressing CCSP for generation of GMAP-compatible promoter

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   Clara Cell Secretory Protein Promoter
   

   Use

   Gmap

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Plac

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. p-mCherry-N1-iChloC

   Plasmid

   #85467

   Purpose

   improved slowChloC artificial anion conducting ChR. Codon optimized for mammalian expression (human/mouse)

   Depositor

   Peter Hegemann

   Article

   Wietek et al Sci Rep. 2015 Oct 7;5:14807. doi: 10.103

   Insert

   iChloC
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   E83Q, E90R, E101S, T159C, D156N

   Promoter

   CMV (+enhancer)

   Available Since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-mWasabi-ER

   Plasmid

   #164165

   Purpose

   Expresses mWasabi fused with an ER-targeting sequence of calreticulin and the ER retrieval sequence, KDEL, under the control of CAG promoter

   Depositor

   Isei Tanida

   Article

   Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

   Insert

   mWasabi
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Feb. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT2K-CAGGS-U6-stuffer-dCAs9-VP160-T2A-IRES-mCherry

   Plasmid

   #114726

   Purpose

   dCas9-VP160 expression in mammalian cells

   Depositor

   Martine Roussel

   Article

   Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1

   Insert

   dCas9-VP160
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. TOPO SV40

    Plasmid

    #68455

    Purpose

    TOPO construct expressing SV40 for generation of GMAP-compatible promoter

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Simian Virus Early 40 Promoter
    

    Use

    Gmap

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. R26-EGFP MMEJ donor vector

    Plasmid

    #137926

    Purpose

    R0SA26 knock-in vector with microhomology-mediated end-joining(MMEJ)-based method

    Depositor

    Hiroshi Kiyonari

    Article

    Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

    Insert

    SA-EGFP-bpA with micro-homology arms
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR-SUMO1

    Plasmid

    #114388

    Purpose

    pDONR vector with SUMO1 gene

    Depositor

    Vincent Dion

    Article

    Aeschbach et al Sci Rep. 2017 Dec 21;7(1):18026. doi: 10

    Insert

    SUMO1 (SUMO1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q29H compared with NCBI reference NP_003343.1

    Promoter

    Available Since

    Aug. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-Trpa1b-FLAG

    Plasmid

    #106403

    Purpose

    Mammalian expression vector containing zebrafish Trpa1b with C-terminal FLAG tag.

    Depositor

    Randall Peterson

    Article

    Lam et al Sci Rep. 2017 Sep 19;7(1):11839. doi: 10

    Insert

    Trpa1b-FLAG (trpa1b Zebrafish)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAS_4xG1 PylT sfGFP 150TAG

    Plasmid

    #154767

    Purpose

    plasmid with 4xG1 PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    150TAG

    Promoter

    EF1

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. FFiBlast MSCV Puro

    Plasmid

    #68464

    Purpose

    Retroviral plasmid for establishment of blasticidin resistance Cre-reporter cell line

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Blasticidin Resistance
    

    Use

    Cre/Lox and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Inverted

    Promoter

    LTR

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBlastR-5xUAS-sfGFP-MiniWhite

    Plasmid

    #165910

    Purpose

    Blasticidin resistant 5xUAS sfGFP Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable UAS Response Vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. CyPet-A206K-PRL2

    Plasmid

    #149685

    Purpose

    For the FRET-based binding assay between human CNNM3 CBS domain and PRL2.

    Depositor

    Motoyuki Hattori

    Article

    Cai et al Sci Rep. 2020 Jul 30;10(1):12879. doi: 1

    Insert

    CyPet-A206K-PRL2
    

    Use

    Tags

    8 histidine tag

    Expression

    Bacterial

    Mutation

    CyPet A206K

    Promoter

    T7

    Available Since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET23a-MmRPS27a

    Plasmid

    #69559

    Purpose

    Bacterial expression; in vitro transcription/translation (T7)

    Depositor

    Jorge Eduardo Azevedo

    Article

    Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

    Insert

    RPS27A (RPS27A Mouse, Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Changed Alanine 133 (human RPS27a) to Glycine (mo…

    Promoter

    T7

    Available Since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pCAG-Bmp15

    Plasmid

    #163594

    Purpose

    Expresses BMP-15 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmp15 (Bmp15 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTorPE-Y-GECO2f

    Plasmid

    #105065

    Purpose

    A yellow fluorescent Ca2+ indicator variant with low binding affinity and fast kinetics for expression in bacterial

    Depositor

    Robert Campbell

    Article

    Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

    Insert

    Y-GECO2f
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available Since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. p63(1-29)-mCherry-N1

    Plasmid

    #67895

    Purpose

    Expresses of fusion of mCherry with the N-terminal 29 amino acids of p63RhoGEF

    Depositor

    Dorus Gadella

    Article

    van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

    Insert

    p63RhoGEF(1-29)-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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22. pETM6-G6-mCherry

    Plasmid

    #66533

    Purpose

    Expresses mCherry under the control of Mutant T7 Promoter G6

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Codon Optimized for E. coli

    Promoter

    Mutant T7 Promoter - G6

    Available Since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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23. pJJF439_PU6_empty_sgRNAscaffold - sgRNA cloning backbone

    Plasmid

    #164266

    Purpose

    sgRNA cloning backbone for expression of sgRNA in C. elegans. Contains BbsI sites for insertion of spacer sequences. U6 promoter from W05B2.8 gene.

    Depositor

    Nikolaus Rajewsky

    Article

    Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6 promoter from W05B2.8

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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24. pEGFP-N1-mSNX27 RNAi-resistant

    Plasmid

    #163620

    Purpose

    mouse SNX27 cDNA with point mutation to resist siRNA against SNX27 C-terminally tagged with GFP

    Depositor

    Josef Kittler

    Article

    Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

    Insert

    mouse SNX27 RNAi-resistant (Snx27 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    RNAi resistance

    Promoter

    Available Since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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25. A3H HapII-Cas9n-UGI

    Plasmid

    #119141

    Purpose

    Base editor made from APOBEC3H Haplotype II

    Depositor

    Reuben Harris

    Article

    Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

    Insert

    APOBEC3H Haplotype II-Cas9 nickase-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    None

    Promoter

    CMV

    Available Since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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26. pCRII-SNORD115-11

    Plasmid

    #45890

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Clin Med Insights Case Rep. 2013 May 5;6

    Insert

    SNORD115 without flanking exons and intron (SNORD115-11 Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

27. MAC_C_EPHA8

    Plasmid

    #187761

    Purpose

    MAC-tagged gene expression of human EPHA8

    Depositor

    Markku Varjosalo

    Article

    Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

    Insert

    EPHA8 (EPHA8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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28. pCAG-Gdf7

    Plasmid

    #163597

    Purpose

    Expresses GDF-7 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Gdf7 (Gdf7 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Some Guanin and Cytosine were substituted to redu…

    Promoter

    Available Since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBAD-BirA-eCPX-AP(K10)

    Plasmid

    #121908

    Purpose

    Expresses BirA and eCPX-AP(K10)

    Depositor

    Per Svenningsen

    Article

    Granhoj et al Sci Rep. 2019 Mar 11;9(1):4118. doi: 10.

    Inserts

    BirA (birA Synthetic)
    enhanced Circularly Permuted Outer Membrane Protein X
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    changed lysine 10 in the AP to alanine

    Promoter

    T7 and pBAD promoter

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET-TALE-mCherry-15b

    Plasmid

    #138287

    Purpose

    Express tTALE-mCherry

    Depositor

    Kyubong Jo

    Article

    Shin et al Sci Rep. 2019 Nov 20;9(1):17197. doi: 10

    Insert

    7-mer tTALE-mCherry
    

    Use

    TALEN

    Tags

    6x His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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