We narrowed to 11,958 results for: pcDNA

1. pcDNA3.1+_FH-AGO2-5xA

   Plasmid

   #92007

   Purpose

   Expresses FLAG-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)]

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Insert

   AGO2 (AGO2 Human)
   

   Use

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   S824A, S828A, T830A, S831A, S834A (5xA)

   Promoter

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCDNA5/FRT-ChemoL-CaM

   Plasmid

   #193830

   Purpose

   CMV overexpression of ChemoL-CaM in mammalian cell lines

   Depositor

   Julien Hiblot

   Article

   Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

   Insert

   ChemoL-CaM
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   EGFP:A206K, E299D; HT7: L271E

   Promoter

   CMV

   Available since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1+_FH-AGO2-5xE

   Plasmid

   #92008

   Purpose

   Expresses FLAG-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)]

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Insert

   AGO2 (AGO2 Human)
   

   Use

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   S824E, S828E, T830E, S831E, S834E (5xE)

   Promoter

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. mScarletI-T2A-mTq2 pcDNA3.1

   Plasmid

   #230048

   Purpose

   Expresses mScarletI-T2A-mTq2 in human cells

   Depositor

   Bas Teusink

   Article

   Botman et al bioRxiv 2024.11.29.626064

   Insert

   mScarletI-T2A-mTq2
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 23, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pcDNAintron-CHIKV-Envelope protein

   Plasmid

   #215700

   Purpose

   Produces the Chikungunya virus envelope protein E3-E2-6K-E1

   Depositor

   Melinda Brindley

   Article

   Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

   Insert

   CHIKV E3-E2-6K-E1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pcDNAintron-CHIKV-capsid protein

   Plasmid

   #215701

   Purpose

   Produces the Chikungunya virus capsid protein

   Depositor

   Melinda Brindley

   Article

   Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

   Insert

   CHIKV capsid
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pcDNA3.1/hChR2(H134R)-EYFP

   Plasmid

   #20940

   Purpose

   Mammalian expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

   Insert

   channelrhodopsin-2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   H134R mutation in ChR2

   Promoter

   CMV

   Available since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1(+)/Puro/FA/GFP-N--Grb2-2

   Plasmid

   #112837

   Purpose

   transient/stable overexpression

   Depositor

   Tomasz Prószyński

   Article

   Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

   Insert

   Grb2 (Grb2 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   SH2 + SH3 (C-term) domains of Grb2

   Promoter

   Available since

   Aug. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCDNA3 Flag Jnk1a1(apf)

   Plasmid

   #13846

   Depositor

   Roger Davis

   Article

   Derijard et al Cell. 1994 Mar 25. 76(6):1025-37.

   Insert

   Jnk1a1(apf) (MAPK8 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   replaced dual phosporylation motif Thr(183)-Pro-T…

   Promoter

   Available since

   Feb. 22, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1-Lari-BFP2-TSERex

    Plasmid

    #123311

    Purpose

    Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

    Depositor

    Yulong Li

    Article

    Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

    Insert

    Lari-BFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCDNA3-Flag MKK6(glu)

    Plasmid

    #13518

    Depositor

    Roger Davis

    Article

    Raingeaud et al Mol Cell Biol. 1996 Mar . 16(3):1247-55.

    Insert

    MKK6(glu) (MAP2K6 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser-207Glu Thr-211Glu

    Promoter

    CMV

    Available since

    July 25, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-HA-CUL1 delta N53

    Plasmid

    #19950

    Depositor

    Yue Xiong

    Article

    Liu et al Mol Cell. 2002 Dec . 10(6):1511-8.

    Insert

    cullin 1 delta N53 (CUL1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    deletion of 53 residues from the NH2-terminus(de…

    Promoter

    Available since

    April 1, 2009

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3-HA-CUL1 delta C22

    Plasmid

    #19942

    Depositor

    Yue Xiong

    Article

    Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

    Insert

    cullin 1 delta C22 (CUL1 Human)
    

    Use

    Tags

    HA2

    Expression

    Mammalian

    Mutation

    Mutation of 22 amino acids from the C terminus

    Promoter

    Available since

    April 17, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA4TO-mito-mCherry-24xGCN4_v1

    Plasmid

    #60913

    Purpose

    A fusion protein of the SunTag 24xGCN4 v1 peptide array to a mitochondrial targeting domain and mCherry

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    mito-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA5/FRT/TO APEX2-GFP

    Plasmid

    #129640

    Purpose

    Express APEX2-GFP fusion in mammalian cells

    Depositor

    Nicholas Ingolia

    Article

    Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

    Insert

    APEX2-GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1-Tat101(AD8)-Flag

    Plasmid

    #115777

    Purpose

    pCMV-Tat101AD8-Flag allows the expression of Tat101(AD8) protein with a C-terminal flag-tag.

    Depositor

    Damian Purcell

    Article

    Khoury et al J Vis Exp. 2019 Jan 22;(143). doi: 10.37

    Insert

    HIV-1 Tat protein (AD8)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.3 ss-3xFLAG-hLRP5 (aa 32-1615)

    Plasmid

    #115788

    Purpose

    Expresses human LRP5 with N-terminal 3xflag tag in mammalian cells, contains artificial signal sequence upstream of tag.

    Depositor

    Harald Junge

    Article

    Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

    Insert

    LRP5 (LRP5 Human)
    

    Use

    Tags

    3xflag

    Expression

    Mammalian

    Mutation

    contains silent mutation that creates an internal…

    Promoter

    CMV

    Available since

    Oct. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1 SARS-CoV-2 S D614G

    Plasmid

    #158075

    Purpose

    Expresses SARS-CoV-2 spike protein with a D614G mutation

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    SARS-CoV-2 spike (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D614G

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-WT-WLS-HA-IRES-GFP

    Plasmid

    #178061

    Purpose

    Expression of WLS-HA protein (WT)

    Depositor

    Joseph Gleeson

    Article

    Chai et al N Engl J Med. 2021 Sep 30;385(14):1292-1

    Insert

    WLS (WLS Human)
    

    Use

    Tags

    HA and IRES-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.3−3xFLAG−AGO2(D669A)

    Plasmid

    #220461

    Purpose

    Express catalytically-dead mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cells

    Depositor

    David Bartel

    Article

    Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

    Insert

    Human AGO2 (AGO2 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Aspartate 669 to alanine

    Promoter

    CMV

    Available since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. FLAG-OTUB1 C91S-pcDNA3.1

    Plasmid

    #118210

    Purpose

    Expresses FLAG-OTUB1 C91S catalytic inactive mutant in mammalian cells

    Depositor

    Cynthia Wolberger

    Article

    Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

    Insert

    OTUB1 (OTUB1 Human)
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    changed cysteine 91 to serine

    Promoter

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3.1-N-Myc_535-1102 USP7

    Plasmid

    #131246

    Purpose

    Mammalian expression of the five USP7 UBL domains with an N-terminal Myc tag.

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Expresses USP7 amino acids 535-1102. This truncat…

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA5/FRT/TO GFP HSPA6

    Plasmid

    #19486

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    heat shock 70kDa protein 6 (HSPA6 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1 Hygro EYFP H148Q

    Plasmid

    #25873

    Depositor

    Peter Haggie

    Article

    Galietta et al FEBS Lett. 2001 Jun 22. 499(3):220-4.

    Insert

    EYFP H148Q
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    EYFP from pEYFP (Clontech) with H148Q mutation

    Promoter

    Available since

    Aug. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

25. α-actinin-4-M522-Venus in pcDNA6

    Plasmid

    #190802

    Purpose

    Imaging of α-actinin-4 in live cells

    Depositor

    Vivian Tang

    Article

    Morris et al J Cell Biol. 2022 May 2;221(5). pii: 213

    Insert

    alpha-actiinin-4-M522-venus (ACTN4 Human, Synthetic)
    

    Use

    Tags

    His, myc, and venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCDNA3-Flag-SPRTN-E112A

    Plasmid

    #110215

    Purpose

    Expression in mammalian cells of of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, and Flag-tag at N-terminus.

    Depositor

    Ivana Marinovic-Terzic, Kristijan Ramadan

    Article

    Lessel et al Nat Genet. 2014 Nov;46(11):1239-44. doi:

    Insert

    SPRTN (SPRTN Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    E112A, P296L (see depositor comments below)

    Promoter

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3. Flag-CSL R192H

    Plasmid

    #167005

    Purpose

    Mammalian expression of CSL R192H

    Depositor

    Gian-Paolo Dotto

    Article

    Bottoni et al Nat Commun. 2019 Aug 29;10(1):3884. doi:

    Insert

    CSL (RBPJ Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    R192H

    Promoter

    CMV

    Available since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1+CMV-Ins-GLuc

    Plasmid

    #89929

    Purpose

    Expresses Insulin-GLuc from the CMV promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.

    Depositor

    Melanie Cobb

    Article

    Kalwat et al ACS Sens. 2016 Oct 28;1(10):1208-1212. E

    Insert

    human insulin-Gaussia-Luciferase (INS Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    DNA sequence for Gaussia luciferase was humanized…

    Promoter

    CMV

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1-TAT-1-101-FLAG

    Plasmid

    #35981

    Depositor

    Susana Valente

    Article

    Mousseau et al Cell Host Microbe. 2012 Jul 19;12(1):97-

    Insert

    transactivating protein (tat )
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    tat from HIV1 101 amino acids

    Promoter

    Available since

    Sept. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. human gamma-catenin pcDNA3

    Plasmid

    #16827

    Depositor

    Eric Fearon

    Article

    Caca et al Cell Growth Differ. 1999 Jun . 10(6):369

    Insert

    gamma-catenin (JUP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Yellow Cameleon-Nano65/pcDNA3

    Plasmid

    #51965

    Purpose

    mammalian expression of Yellow Cameleon-Nano65, an ultrasensitive Ca2+ indicator with Kd = 65 nM

    Depositor

    Takeharu Nagai

    Article

    Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

    Insert

    Yellow Cameleon-Nano65
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

32. pcDNA5/FRT miniAID-EGFP

    Plasmid

    #101713

    Purpose

    For amplification of a miniAID-EGFP for C-terminal tagging

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    miniAID-EGFP
    

    Use

    Tags

    miniAID-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA4-TO-Puromycin-mVenus-MAP

    Plasmid

    #44118

    Depositor

    Dannel McCollum

    Article

    Ma et al Mol Cell Proteomics. 2012 Aug;11(8):501-

    Type

    Empty backbone

    Use

    Tags

    mVenus-MAP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3-HCMVgL-FLAG (TB40/E)

    Plasmid

    #136449

    Purpose

    Mammalian expression plasmid for HCMV glycoprotein L (TB40/E strain) with FLAG tag

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    glycoprotein L (UL115 Human betaherpesvirus 5)
    

    Use

    Tags

    Short SGGSG linker and FLAG tag

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3.1-mas-GRK2RH-Nluc

    Plasmid

    #158605

    Purpose

    Plasmid expressing GRK2(RH)-Nluc in mammalian cells

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - GRK2 RH domain - linker (GGTGGS) - Nluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 29, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. pCDNA5/avitag-apex2-v5-fkbp8

    Plasmid

    #154460

    Purpose

    expresses avitag-apex2-v5-fkbp8/biochemical assay

    Depositor

    Hyun-Woo Rhee

    Article

    Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

    Insert

    avitag-apex2-v5-fkbp8 (FKBP8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-F, T7 promoter

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3 deltaN47 Beta-catenin

    Plasmid

    #19287

    Depositor

    Eric Fearon

    Article

    Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

    Insert

    beta-catenin (CTNNB1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    N-terminal truncation, deleted aa 1-47

    Promoter

    Available since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA5-FRT-GFP-MYC

    Plasmid

    #185375

    Purpose

    For mammalian expression of human GFP-MYC

    Depositor

    Ayaz Najafov

    Insert

    MYC (MYC Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3.1-nAChr-Alpha6-GFP

    Plasmid

    #50486

    Purpose

    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

    Depositor

    Henry Lester

    Article

    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

    Insert

    nAChr-alpha6 (Chrna6 Mouse)
    

    Use

    Tags

    GFP fusion in M3-M4 loop (after residue A405)

    Expression

    Mammalian

    Mutation

    Gly-Ala-Gly flexible linker flanking the GFP open…

    Promoter

    CMV

    Available since

    Jan. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

40. pcDNA5/FRT/TO-GAlphaz-RLuc8

    Plasmid

    #140978

    Purpose

    Encodes a G alpha subunit (GNAZ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphaz-Rluc8 (GNAZ Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge