We narrowed to 17,876 results for: URE

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pEPSA5Cas9syn2

Plasmid

#206963

Purpose

S. aureus genome editing vector removed these two motifs AGGNNNNNGAT, CCAYNNNNNNTGT, CCAYNNNNNNGTA, GGANNNNNNNNTCG, and GWAGNNNNNNGAT

Depositor

Daijie Chen

Article

Yang et al ACS Synth Biol. 2023 Oct 13. doi: 10.102

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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His6-Twin-Strep-SUMO-pET28a-EfaCas2

Plasmid

#112794

Purpose

To express enterococcus faecalis Cas2 protein in E. coli

Depositor

Ailong Ke

Article

Xiao et al Nature. 2017 Oct 5;550(7674):137-141. do

Insert

EfaCas2

Tags

His6-Twin-Strep-SUMO

Expression

Bacterial

Available Since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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CAGhP14

Plasmid

#84362

Purpose

express FLAG-HA tagged human PRDM14

Depositor

Danny Reinberg

Article

Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

Insert

PRDM14 (PRDM14 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

silent mutations at coding silent 1068CtoT, 1629T…

Promoter

CAG

Available Since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only
untargeted Plk1 FRET sensor Myt1 substrate

Plasmid

#45217

Depositor

Michael Lampson

Article

Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

Insert

untargeted Plk1 FRET sensor Myt1 substrate

Expression

Mammalian

Promoter

t7

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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Ki67∆repeats-mNeonGreen-IRESpuro2

Plasmid

#183742

Purpose

Expresses Ki67∆repeats-mNeonGreen

Depositor

Daniel Gerlich

Article

Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

Insert

MKI67(lacking repeats) (MKI67 Human)

Tags

mNeonGreen

Expression

Bacterial and Mammalian

Mutation

MKI67(lacking repeats)

Promoter

CMV

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pT7C-HSPA1L

Plasmid

#177660

Purpose

Recombinant protein synthesis of human HSPA1L

Depositor

Jeff Kuret

Article

Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

Insert

HSPA1L (HSPA1L Human)

Tags

6xHis-Thrombin

Expression

Bacterial

Promoter

T7

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pCsC_spacer

Plasmid

#136077

Purpose

Use when making odd level constructs (L3,...) if pCsC position (position 3) has no construct assigned

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Even spacer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCsE_spacer

Plasmid

#136078

Purpose

Use when making odd level constructs (L3,...) if pCsE position (position 4) has no construct assigned

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Even spacer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCsB

Plasmid

#136068

Purpose

Acceptor plasmid for even level position 2, lacZ blue-white screening

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Use

Synthetic Biology

Expression

Plant

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCsC

Plasmid

#136069

Purpose

Acceptor plasmid for even level position 3, lacZ blue-white screening

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Use

Synthetic Biology

Expression

Plant

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCsE

Plasmid

#136070

Purpose

Acceptor plasmid for even level position 4, lacZ blue-white screening

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Use

Synthetic Biology

Expression

Plant

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pAB094e

Plasmid

#79245

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PBAD rpoZ-HA4 (Y87A); PC araC

Expression

Bacterial

Promoter

PBAD; PC

Available Since

May 12, 2018

Availability

Academic Institutions and Nonprofits only

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pDDGFP-Leu2d-ScVrg4

Plasmid

#102334

Purpose

Expresses WT ScVrg4 in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Article

Parker et al Nature. 2017 Nov 23;551(7681):521-524. d

Insert

YGL225W (VRG4 Budding Yeast)

Tags

GFP

Expression

Yeast

Promoter

GAL1

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pEPSA5Cas9syn

Plasmid

#206965

Purpose

S. aureus genome editing vector removed these two motifs AGGNNNNNGAT and CCAYNNNNNNTGT

Depositor

Daijie Chen

Article

Yang et al ACS Synth Biol. 2023 Oct 13. doi: 10.102

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAWH

Plasmid

#133894

Purpose

Expression of Gal4AD-Prey hybrid protein. Homology regions for recombination with pBKWH, pBSWHhc or pMS22H

Depositor

Sebastian Maurer

Article

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Type

Empty backbone

Expression

Yeast

Available Since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

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pBKWH

Plasmid

#133898

Purpose

Expression of Gal4BD-Bait hybrid protein. Homology regions for recombination with pAWH

Depositor

Sebastian Maurer

Article

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Type

Empty backbone

Expression

Yeast

Available Since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-mTUT5

Plasmid

#60042

Purpose

Mammalian expression of FLAG-tagged mouse TUT5

Depositor

Zissimos Mourelatos

Article

Liu et al Mol Cell. 2014 Aug 27. pii: S1097-2765(1

Insert

TUT5 (Papd7 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2014

Availability

Academic Institutions and Nonprofits only
pAB045a

Plasmid

#79224

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ rpoZ-HA4; PlacIq lacI

Expression

Bacterial

Promoter

PlacZ; PlacIq

Available Since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-T7-hAIM2-EGFP

Plasmid

#51608

Purpose

Express T7 and GFP-tagged hAIM2 in mammalian cells

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

AIM2 (AIM2 Human)

Use

Retroviral

Tags

EGFP and T7

Expression

Mammalian

Promoter

MESV

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-GFP-LC3-mRuby3

Plasmid

#184898

Purpose

Stable expression of GFP-LC3-mRuby3 in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Article

Okawa et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

LC3B

Tags

EGFP and mRuby3

Expression

Mammalian

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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