We narrowed to 5,821 results for: ARL

1. EFR Y877F (17)

   Plasmid

   #102415

   Purpose

   fluorescent tagging. Plant expression of EFR Y877F (17)

   Depositor

   Silke Robatzek

   Insert

   EFR (EFR Mustard Weed)
   

   Use

   Tags

   6xHis and mgfp5

   Expression

   Plant

   Mutation

   Y877F

   Promoter

   35S

   Available since

   Nov. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. EFR Y875F (15)

   Plasmid

   #102414

   Purpose

   fluorescent tagging. Plant expression of EFR Y875F (15)

   Depositor

   Silke Robatzek

   Insert

   EFR (EFR Mustard Weed)
   

   Use

   Tags

   6xHis and mgfp5

   Expression

   Plant

   Mutation

   Y875F

   Promoter

   35S

   Available since

   Nov. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV.EF1a.ChR2-YFP.WPRE.hGH

   Plasmid

   #100056

   Purpose

   AAV expression of ChR2 fused to YFP driven byEF1a promoter for optogenetic activation

   Depositor

   Karl Deisseroth

   Insert

   ChR2
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRVdG-4RFP-5premGRASP

   Plasmid

   #52499

   Purpose

   Expresses TagRFP-T and presynaptic mGRASP component

   Depositor

   Ian Wickersham

   Inserts

   TagRFP-T
   presynaptic mGRASP
   

   Use

   Rabies virus

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRVdG-4BFP2-5postmGRASP

   Plasmid

   #52498

   Purpose

   Expresses mTagBFP2 and postsynaptic mGRASP component

   Depositor

   Ian Wickersham

   Inserts

   mTagBFP2
   postsynaptic mGRASP
   

   Use

   Rabies virus

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRVdG-4Halo3-mCherry

   Plasmid

   #59327

   Purpose

   Expresses eNpHR 3.0-mCherry

   Depositor

   Ian Wickersham

   Insert

   eNpHR 3.0-mCherry
   

   Use

   Rabies virus

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRVdG-4ChR2-mCherry

   Plasmid

   #59328

   Purpose

   Expresses ChR2-mCherry

   Depositor

   Ian Wickersham

   Insert

   ChR2-mCherry
   

   Use

   Rabies virus

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   H134R

   Promoter

   CMV

   Available since

   Sept. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pENTR CMVie-Lifeact-mTagBFP2 L1-R5

   Plasmid

   #84388

   Purpose

   Multisite Gateway entry vector for expression of mTagBFP2-tagged Lifeact in cells including neurons

   Depositor

   Ghassan Mouneimne

   Insert

   Lifeact
   

   Use

   Gateway

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV immediate early

   Available since

   Jan. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pENTR CMVie-Lifeact-mCardinal L1-R5

   Plasmid

   #84392

   Purpose

   Multisite Gateway entry vector for expression of mCardinal-tagged Lifeact in cells including neurons

   Depositor

   Ghassan Mouneimne

   Insert

   Lifeact
   

   Use

   Gateway

   Tags

   mCardinal

   Expression

   Mammalian

   Mutation

   Promoter

   CMV immediate early

   Available since

   Jan. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-Ef1a-DIO EYFP

    Plasmid

    #27056

    Depositor

    Karl Deisseroth

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, and AAV9

    Insert

    EYFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-EF1a-double floxed-mCherry-WPRE-HGHpA

    Plasmid

    #20299

    Purpose

    Cre-activated AAV expression of mCherry

    Depositor

    Karl Deisseroth

    Insert

    mCherry
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMCMV3

    Plasmid

    #85711

    Purpose

    Expression vector employing the mouse CMV major immediate-early promoter enhancer (MIEP); there is a small polylinker (PstI, SmaI, XbaI, SalI ,HpaI) followed by the SV40 polyA signal sequences

    Depositor

    Martin Messerle, Ulrich Koszinowski

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MIEPmouse CMV MIEP

    Available since

    April 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBabepuro-PDGFB

    Plasmid

    #189611

    Purpose

    Retroviral expression vector for PDGFB. stimulates fibroblastic proliferation (Tchenio T, unpublished, 2008)

    Depositor

    Thierry Tchenio

    Insert

    PDGFB (PDGFB Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    SV40 early promoter

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Plenti-CMV-MCS-RFP-SV-puro

    Plasmid

    #109377

    Purpose

    This plasmid expresses dsRed2 and puromycin resistance

    Depositor

    Jonathan Garlick, Behzad Gerami-Naini

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    dsRed2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-GFAP-hChR2(H134R)-EYFP

    Plasmid

    #27054

    Purpose

    AAV expression of humanized ChR2(H134R) fused to EYFP, for optogenetic excitation

    Depositor

    Karl Deisseroth

    Insert

    hChR2(H134R)
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    GFAP

    Available since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-GFAP-hChR2(H134R)-mCherry

    Plasmid

    #27055

    Purpose

    AAV expression of humanized ChR2(H134R) fused to mCherry, for optogenetic excitation

    Depositor

    Karl Deisseroth

    Insert

    hChR2(H134R)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    GFAP

    Available since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pInt_attP1_sacB_kanR

    Plasmid

    #205301

    Purpose

    Integrating plasmid for ORBIT in E. coli with sacB for counter selection. Used for markerless or scarless modifications.

    Depositor

    Scott Saunders

    Article

    Saunders et al bioRxiv 2023

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pOpen-T4gene30

    Plasmid

    #165554

    Purpose

    Ligase derived from T4 bacteriophage expressed in the early stage of lytic development. Has been implicated in T4 DNA synthesis and genetic recombination. May also play a role in T4 DNA repair.

    Depositor

    Jennifer Molloy, FreeGenes Project, Drew Endy

    Insert

    T4-DNA Ligase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 16, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. tetO-GFPnls-mPGK-rtTA-IRES-Puro

    Plasmid

    #69545

    Purpose

    Tet-On system expressing GFP tagged with 2x nuclear localization signals.

    Depositor

    Charles Gersbach

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    2x nuclear localization sequence and T2A "sk…

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-hSyn-fDIO-EYFP-WPRE

    Plasmid

    #154870

    Purpose

    Human synapsin-1 promoter; Flp-dependent EYFP

    Depositor

    Ulrik Gether

    Insert

    EYFP
    

    Use

    AAV and Mouse Targeting; Flp-frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human synapsin promoter

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBabepuro-IL11

    Plasmid

    #189609

    Purpose

    Retroviral expression vector for IL11

    Depositor

    Thierry Tchenio

    Insert

    IL11 (IL11 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    SV40 early promoter

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pJF3H

    Plasmid

    #63897

    Purpose

    For phage display of cDNA libraries

    Depositor

    Carlos Barbas

    Article

    Barbas et al Cold Spring Harbor, NY: Cold Spring Harb

    Insert

    cDNA cloning region
    

    Use

    Phage display

    Tags

    Fos

    Expression

    Bacterial

    Mutation

    Promoter

    lacZ

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3-HA-SOX4

    Plasmid

    #110367

    Purpose

    Mammalian expression of HA-tagged SOX4

    Depositor

    Carlos Moreno

    Insert

    SOX4 (SOX4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-nEF Con/Fon DREADD Gq-mCherry

    Plasmid

    #183532

    Purpose

    INTRSECT Chemogenetics - expresses DREADD Gq-mCherry in cells expressing Cre AND Flp

    Depositor

    Karl Deisseroth

    Insert

    DREADD Gq-mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    None

    Promoter

    nEF

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-nEF Coff/Fon DREADD Gq-mCherry

    Plasmid

    #183534

    Purpose

    INTRSECT Chemogenetics - expresses DREADD Gq-mCherry in cells expressing Flp but not Cre

    Depositor

    Karl Deisseroth

    Insert

    DREADD Gq-mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    None

    Promoter

    nEF

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Phage-PGK-GFP-T(WT)

    Plasmid

    #181736

    Purpose

    for lentiviral expression of brachyury ORF in mammalian cells

    Depositor

    Charles Lin

    Insert

    T (brachyury)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    a silent mutation in the PAM site of an sgRNAt ta…

    Promoter

    Available since

    Aug. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBabepuro-IL8

    Plasmid

    #189608

    Purpose

    Retroviral expression vector for IL8

    Depositor

    Thierry Tchenio

    Insert

    IL8 (CXCL8 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    SV40 early promoter

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCAS9i_TRP

    Plasmid

    #141253

    Purpose

    Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

    Depositor

    Adam Bertl

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 (Cas9), pSNR52 (gRNA)

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-nEF Con/Foff DREADD Gq-mCherry

    Plasmid

    #183533

    Purpose

    INTRSECT Chemogenetics - expresses DREADD Gq-mCherry in cells expressing Cre but not Flp

    Depositor

    Karl Deisseroth

    Insert

    DREADD Gq-mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    None

    Promoter

    nEF

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-EF1a-double floxed-EYFP-WPRE-HGHpA

    Plasmid

    #20296

    Purpose

    Cre-activated AAV expression of EYFP

    Depositor

    Karl Deisseroth

    Insert

    EYFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDest-Tol2 (Chien)-Krt4:Gal4VP16:tdTomato:5xUAS_MCS

    Plasmid

    #105649

    Purpose

    tdTomato expression in differentiated keratinocytes of the periderm layer and during early larval stages also in the basal layer of the epidermis

    Depositor

    Sandra Rieger

    Insert

    Krt4:Gal4VP16:tdTomato:5xUAS_MCS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

32. Phage-PGK-FLAG-HA-T(WT)

    Plasmid

    #181737

    Purpose

    for lentiviral expression of brachyury ORF in mammalian cells

    Depositor

    Charles Lin

    Insert

    T (brachyury)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    a silent mutation in the PAM site of an sgRNAt ta…

    Promoter

    Available since

    Aug. 3, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. pCI-ING5

    Plasmid

    #79071

    Purpose

    Expression of ING5 in mammalian cells

    Depositor

    Karl Riabowol

    Insert

    ING5 (ING5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

34. pT7CFE1_EVA71_VP12A

    Plasmid

    #212977

    Purpose

    Encodes EV-A71 4643 VP12A optimized for expression in mammalian transcription/translation extracts

    Depositor

    Karla Kirkegaard

    Insert

    Enterovirus A71 4643 VP12A
    

    Use

    Mammalian cell free protein expression

    Tags

    HA tag

    Expression

    Mutation

    Promoter

    T7

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pT7CFE1_EVD68_VP12A

    Plasmid

    #212984

    Purpose

    Encodes EV-D68 MO/14/18947 VP12A optimized for expression in mammalian transcription/translation extracts

    Depositor

    Karla Kirkegaard

    Insert

    EV-D68 MO/14/18947 VP12A
    

    Use

    Mammalian cell free protein expression

    Tags

    HA

    Expression

    Mutation

    Promoter

    T7

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pT7CFE1_EVD68_3ABC

    Plasmid

    #212979

    Purpose

    ncodes EV-D68 MO/14/18947 3ABC optimized for expression in mammalian transcription/translation extracts

    Depositor

    Karla Kirkegaard

    Insert

    EV-D68 MO/14/18947 3ABC
    

    Use

    Mammalian cell free protein expression

    Tags

    HA

    Expression

    Mutation

    Promoter

    T7

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pT7CFE1_EVD68_3ABCD

    Plasmid

    #212981

    Purpose

    Encodes EV-D68 MO/14/18947 3ABCD optimized for expression in mammalian transcription/translation extracts

    Depositor

    Karla Kirkegaard

    Insert

    EV-D68 MO/14/18947 3ABCD
    

    Use

    Mammalian cell free protein expression

    Tags

    HA

    Expression

    Mutation

    Promoter

    T7

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCAS9id_TRP

    Plasmid

    #141254

    Purpose

    Galactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassettes with stuffer and Kpn restriction sites.

    Depositor

    Adam Bertl

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 (Cas9), pSNR52 (gRNA)

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pZDonor-U6-myogenin stop codon gRNA

    Plasmid

    #69549

    Purpose

    Expresses guide RNA targeting near the stop codon of myogenin in the mouse genome.

    Depositor

    Charles Gersbach

    Insert

    Myogenin C terminus-targeting guide RNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAAV-hSyn-hChR2(H134R)-mCherry

    Plasmid

    #26976

    Purpose

    AAV expression of humanized ChR2 with H134R mutation fused to mCherry, for optogenetic excitation

    Depositor

    Karl Deisseroth

    Has Service

    AAV5, AAV8, and AAV9

    Insert

    hChR2(H134R)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    hSyn

    Available since

    Feb. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge