We narrowed to 34,416 results for: CaS;

1. pCJH065_proD_Cas12c_DualRNA-GFP+HDVrz_CAM

   Plasmid

   #183077

   Purpose

   Plasmid expressing Cas12c tracrRNA and GFP-targeting crRNA separately, with the crRNA ending with a HDV ribozyme sequence.

   Depositor

   Jennifer Doudna

   Article

   Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

   Insert

   Cas12c tracrRNA and crRNA ending with a HDV ribozyme
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. eSpCas9(1.1)_No_FLAG_ATP1A1_G3

   Plasmid

   #86611

   Purpose

   Expresses the ATP1A1 G3 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 intron 4. Px330-like plasmid

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

   Insert

   ATP1A1 G3 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
   

   Use

   CRISPR; Co-selection via hdr using ouabain

   Tags

   Expression

   Mammalian

   Mutation

   K848A, K1003A, & R1060A

   Promoter

   CBh

   Available since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSpCas9(BB)-2A-GFP-G3BP2(2)

   Plasmid

   #136061

   Purpose

   G3BP2 gRNA (#2) inserted into the pSpCas9(BB)-2A-GFP plasmid (GACAACTACTCCATCACTCA)

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   G3BP2 (G3BP2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. RCAS(A) mTcf4B (CT#1126)

   Plasmid

   #14019

   Depositor

   Cliff Tabin

   Article

   Kardon et al Dev Cell. 2003 Dec . 5(6):937-44.

   Insert

   Tcf4B (Tcf7l2 Mouse)
   

   Use

   Retroviral; Avian expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 16, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pYPQ-dAaCas12b-Act3.0

   Plasmid

   #158413

   Purpose

   CRISPR-Act3.0 system containing dAaCas12b-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Insert

   dAaCas12b-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330_ACTB-i1 sgRNA / hSpCas9

   Plasmid

   #172825

   Purpose

   Mammalian expression of a sgRNA targeting the intron 1 position 1 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX459-HypaCas9-TUBA1B_sgRNA

   Plasmid

   #183889

   Purpose

   pX459V2.0-HypaCas9 plasmid with TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   TUBA1B sgRNA spacer (TUBA1B Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBLO43.3.-6_human ProCas9Flavi-6

   Plasmid

   #121630

   Purpose

   U6-sgRNAdest_CMV-intron_hProCas9Flavi-6_T2A Mcherry_AmpR_ColE1

   Depositor

   David Savage

   Article

   Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCaSpeR4-tubulin-QF#7m1

   Plasmid

   #46128

   Depositor

   Christopher Potter

   Article

   Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

   Insert

   QF#7m1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pST1374-dCas9-GCN4

    Plasmid

    #113026

    Purpose

    Expresses dCas9-GCN4 in mammalian cells

    Depositor

    Xingxu Huang

    Article

    Jiang et al Cell Res. 2018 Jun 6. pii: 10.1038/s4142

    Insert

    dCas9-GCN4 (GCN4 Budding Yeast, S. pyogenes)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A, N863A

    Promoter

    CMV

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAGT8162 (AATG_dttLbCas12a-i_TTCG)

    Plasmid

    #192350

    Purpose

    L0 MoClo-compatible GoldenGate-module of dttLbCas12a-i for in planta expression

    Depositor

    Tom Schreiber

    Article

    Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

    Insert

    AATG_dttLbCas12a-i_TTCG
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D156R; E925Q

    Promoter

    Available since

    Nov. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. p3s-Cas9-HF1

    Plasmid

    #104173

    Purpose

    Cas9-HF1 mammalian expression

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

    Insert

    Cas9-HF1
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, Q926A

    Promoter

    CMV

    Available since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCaSpeR-act5cB-LexAQF

    Plasmid

    #61310

    Purpose

    Expresses chimeric transactivator LexA_DBD:QF2_AD in Drosophila

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Insert

    LexA_DBD::QF2_AD
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. RCAS (A) Six1 (CT#664)

    Plasmid

    #13876

    Depositor

    Cliff Tabin

    Article

    Heanue et al Genes Dev. 1999 Dec 15. 13(24):3231-43.

    Insert

    Six1 (Six1 Mouse)
    

    Use

    Retroviral; Avian expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

15. p3s-eCas9-1.1

    Plasmid

    #104172

    Purpose

    eCas9-1.1 mammalian expression

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

    Insert

    eCas9-1.1
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K848A, K1003A, R1060A

    Promoter

    CMV

    Available since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mCherry-CCHD (p130Cas)

    Plasmid

    #145133

    Purpose

    Expresses CCH domain from p130Cas with an mCherry tag

    Depositor

    Johanna Ivaska

    Article

    Jacquemet et al Curr Biol. 2019 Jan 21;29(2):202-216.e7.

    Insert

    p130Cas CCH domain (BCAR1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Lenti Cas9/SB100

    Plasmid

    #155294

    Purpose

    Lentiviral expression of Cas9 and SB100

    Depositor

    Martin Carroll

    Insert

    SB100X-P2A-spCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pACYC-Cas12c2-noncoding

    Plasmid

    #120877

    Purpose

    Expresses concatenated noncoding sequences surrounding the CRISPR-Cas12c2 system in the native genomic loci; expresses the tracrRNA required for activity.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

    Insert

    Noncoding sequences for CRISPR-Cas12c2
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR221 Cas9-RFC5

    Plasmid

    #183200

    Purpose

    Entry clone

    Depositor

    Emma Haapaniemi

    Article

    Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

    Insert

    Cas9-RFC5
    

    Use

    CRISPR; Gateway entry clone

    Tags

    Expression

    Mutation

    n/a

    Promoter

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSpCas9_BB_2A-GFP_MAPRE1-gRNA#2

    Plasmid

    #107727

    Purpose

    Knock out of EB1 in human cells by CRISPR/Cas9

    Depositor

    Torsten Wittmann

    Article

    van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

    Insert

    MAPRE1 gRNA #1 (targets Exon 1) (MAPRE1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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