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pHSB04X

Plasmid

#117258

Purpose

Genome editing for Lactobacillus brevis ATCC367

Depositor

Sheng Yang

Publication

Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

Insert

Cas9, promotor PslpA-guide-RNA, homologous arms of Lb_1019

Use

Other

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pX330-SpCas9-HF1

Plasmid

#108301

Purpose

pX330 (Plasmid #42230) with the N497A, R661A, Q695A, and Q926A mutations

Depositor

Yuichiro Miyaoka

Publication

Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pX458-DsRed2

Plasmid

#112219

Purpose

Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and DsRed2

Depositor

Yinming Liang

Publication

Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:

Insert

DsRed2

Use

CRISPR

Expression

Mammalian

Mutation

DsRed2 contains a silent nucleotide substitution …

Available since

July 11, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pnsgRNA

Plasmid

#172717

Purpose

Delivery of guide RNA for prime editing

Depositor

Tilmann Weber

Publication

Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

Insert

J23119-gRNA

Expression

Bacterial

Available since

Sept. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pH6-MBP-TEV-BirA

Plasmid

#179694

Purpose

Expresses His6 tagged MBP-BirA fusion protein

Depositor

Mark Arbing

Insert

BirA

Tags

His6-MBP

Expression

Bacterial

Available since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_antiHer24D5-3_synNotch_Gal4VP64

Plasmid

#85422

Purpose

Lentiviral vector for expression of anti-Her2 scFv 24D5-3 Gal4VP64 synthetic Notch

Depositor

Wendell Lim

Publication

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

anti-Her_4D5-3 synNotch

Use

Lentiviral

Promoter

PGK

Available since

Nov. 2, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAW005-2

Plasmid

#85611

Purpose

Empty gRNA delivery vector for Bacillus subtilis

Depositor

Perry Chou

Publication

Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

Type

Empty backbone

Expression

Bacterial

Available since

Jan. 6, 2017

Availability

Academic Institutions and Nonprofits only

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plentiCMV-sct-pp65-b2m-HLA-A0201

Plasmid

#196511

Purpose

Express single-chain peptide-MHC (pp65 CMV epitope on HLA-A0201)

Depositor

Howard Chang

Publication

Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

Insert

single chain of a pp65 peptide, b2m and HLA A0201

Use

Lentiviral

Tags

NA

Expression

Mammalian

Mutation

Y84C

Promoter

CMV

Available since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-flex-oChIEF-citrine

Plasmid

#50973

Purpose

flexed oChIEF-citrine construct in an AAV transfer vector containing AAV2 ITR

Depositor

Roger Tsien

Publication

Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

Insert

ChIEF-citrine

Use

AAV

Tags

citrine

Mutation

inverted and inserted between two lox sites

Promoter

human synapsin promoter

Available since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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1437 pSG5L Flag HA PTEN

Plasmid

#10786

Depositor

William Sellers

Insert

PTEN (PTEN Human)

Tags

Flag and HA

Expression

Mammalian

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
scAAV-Cre

Plasmid

#177933

Purpose

Expresses Cre-recombinase and can be packaged into AAV particles.

Depositor

Roland Rad

Publication

Kaltenbacher et al Nat Protoc. 2022 Apr;17(4):1142-1188. do

Insert

CAG-Cre

Use

AAV

Expression

Mammalian

Promoter

U6

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-GFAP-mKate2.5f

Plasmid

#99129

Purpose

Also known as pAAV-GFAP-mKate2.5-f. An AAV genome that express the farnesylated fluorescent protein mKate2.5 from the GFAP promoter

Depositor

Viviana Gradinaru

Publication

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mKate2.5f

Use

AAV

Expression

Mammalian

Promoter

GFAP

Available since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pZHY013

Plasmid

#36185

Depositor

Daniel Voytas

Publication

Zhang et al Plant Physiol. 2012 Nov 2.

Insert

Heterodimeric FokI nuclease

Use

Entry vector for expression in plants as a t2a li…

Promoter

none

Available since

Aug. 24, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pG1K

Plasmid

#71741

Purpose

Modular shuttle vector for Geobacillus and E. coli

Depositor

Tom Ellis

Publication

Reeve et al ACS Synth Biol. 2016 Jul 1.

Type

Empty backbone

Use

Synthetic Biology; Shuttle vector

Expression

Bacterial

Available since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACE2-WT(732)-IgG1

Plasmid

#154104

Purpose

Mammalian expression plasmid for dimeric soluble ACE2 (IgG1-Fc fusion)

Depositor

Erik Procko

Publication

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Tags

Fc region of human IgG1

Expression

Mammalian

Mutation

Extracellular, soluble protease and neck domains …

Promoter

CMV

Available since

June 24, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pTD-CTwinStrep

Plasmid

#45939

Purpose

use to create C-terminal Twin-Strep-tag fusion proteins

Depositor

Thorben Dammeyer

Publication

Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

Type

Empty backbone

Tags

Twin-Strep

Expression

Bacterial

Promoter

Trc (lactose/IPTG inducible)

Available since

Sept. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pBR.DT-A

Plasmid

#35955

Depositor

Jacqueline Lees

Publication

Danielian et al Oncogene. 2008 Nov 20;27(51):6561-70. Ep

Insert

diphtheria toxin A

Use

Bac recombineering

Promoter

PGK

Available since

April 19, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCRISPR-dCas9

Plasmid

#125687

Purpose

Gene knockdown for actinomycetes

Depositor

Tilmann Weber

Publication

Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

Insert

streptomyces codon optimized spdCas9, sgRNA casette

Use

CRISPR and Synthetic Biology

Promoter

ermE*/tipA

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3.1_iSH2-pMagFast2(3x)-iRFP

Plasmid

#67298

Purpose

Blue light dependent photoswitching system

Depositor

Moritoshi Sato

Publication

Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

Insert

iSH2-pMagFast2(3x)-iRFP (PIK3R1 Human, Synthetic)

Expression

Mammalian

Promoter

CMV

Available since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pIRES2-eGFP-Ofd1-Myc

Plasmid

#24560

Depositor

Jeremy Reiter

Publication

Singla et al Nat Methods. 2010 Jan . 7(1):50-2.

Insert

Ofd1 (Ofd1 Mouse)

Tags

Myc and eGFP

Expression

Mammalian

Available since

May 20, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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