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Addgene

We narrowed to 9,754 results for: Gnas

Showing: 5351 - 5400 of 9754 results
  1. pSLQ8666 pHR-RRM2p-BFP-T2A-N term (1-406) dLbCpf1-EE12RR345L-EFS-Zeo-WPRE

    Plasmid
    #140228
    Purpose
    RRM2p-driven expression of BFP and N terminal dCpf1 (Cas12a) 406-split half with leucine zipper. Constitutive EFS-driven zeocin resistance.
    Depositor
    Insert
    dLbCpf1 (Cas12a) N terminal half (406) with zipper
    Use
    Lentiviral
    Tags
    BFP
    Expression
    Mammalian
    Mutation
    Promoter
    RRM2 endogenous promoter
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
  2. pcDNA3-pmEMBer

    Plasmid
    #174441
    Purpose
    Plasma membrane-targeted ERK monobody binder for local inhibition of ERK activity in live cells.
    Depositor
    Insert
    pmEMBer
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
  3. nLuc_AP10_PPVs_P9mS

    Plasmid
    #119302
    Purpose
    Expresses N-terminus of split luciferase fused to antiparallel coiled-coil AP10 connected with autoinhibitory coil P9mS and PPVs cleavage site in antiparallel harpin linker/for SPOC logic
    Depositor
    Insert
    split nLuc fused to antiparallel Coiled-coil AP10 connected with P9mS via PPV cleavable linker
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
  4. pSOMA-T679A-NLS

    Plasmid
    #118938
    Purpose
    Used to express the T679A mutant form of the SOMA FRET sensor with a nuclear localization signal in Arabidopsis thaliana
    Depositor
    Insert
    SOMA Sensor for Arabidopsis MAPK activity with T679A Mutation and Nuclear Localization Signal
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
  5. LIC-Z

    Plasmid
    #153543
    Purpose
    perform light induced optogenetic clustering of TCR ζ-Chain as described in the associated publication
    Depositor
    Insert
    human TCR ζ-Chain (CD247 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    NA
    Promoter
    CMV
    Available since
    July 17, 2020
    Availability
    Academic Institutions and Nonprofits only
  6. mEos3.2trCD4o

    Plasmid
    #203751
    Purpose
    Encodes the transmembrane domain from CD4, including its 2 palmitoylation sites, with mEos3.2 fused on the N terminus. Used as a fluorescent plasma membrane probe.
    Depositor
    Insert
    trCD4o (CD4 Human)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
  7. pEVA1

    Plasmid
    #58953
    Purpose
    Contains a synthetic DNA sequence, VAR1u, that specifies the Var1 protein. The Cox4 targeting signal is fused to the N-terminus to direct import of Var1. Complements var1 mutations in mtDNA.
    Insert
    VAR1u, VAR1 recoded for expression from the nucleus
    Use
    Yeast ecoli shuttle vector
    Tags
    preCox4 mitochondrial matrix targeting signal
    Expression
    Yeast
    Mutation
    Synthetic gene in standard genetic code
    Promoter
    ADH1
    Available since
    Aug. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
  8. nLuc_AP4_TEVs_P3mS

    Plasmid
    #119299
    Purpose
    Expresses N-terminus of split luciferase fused to antiparallel coiled-coil AP4 connected with autoinhibitory coil P3mS and TEV cleavage site in antiparallel harpin linker/for SPOC logic
    Depositor
    Insert
    split nLuc fused to antiparallel Coiled-coil AP4 connected with P3 via TEV cleavable linker
    Use
    Luciferase
    Tags
    Myc tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
Showing: 5351 - 5400 of 9754 results