59,588 results
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SpCas9-NG-ABE8.17-m+V106W
Plasmid#198534PurposeExpresses human codon-optimized SpCas9-NG-ABE8.17-m+V106W and blasticidin resistance: EFS promoter-SpCas9-NG-ABE8.17-m+V106W-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NG-ABE8.17-m+V106W
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianPromoterEFSAvailable SinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pInducer-myc-ERK2-R67S/S153D/D321N
Plasmid#213598PurposeDoxycycline inducible expression of myc-tagged ERK2-R67S/S153D/D321N cDNA in mammilian cellsDepositorAvailable SinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRC1 CTF-FLAG
Plasmid#217684PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertCELSR1 (CELSR1 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-2447Available SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-MUTHY
Plasmid#215132PurposeExpresses mCherry-MUTHY in mammalian cells from a retroviral vector.DepositorAvailable SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-C_WDR13:1-485
Plasmid#211162PurposeMammalian expression of full-length human WDR13. C-terminal HiBiT tag.DepositorInsertWDR13:M1-K485 (WDR13 Human)
TagsGSSGGSSGVSGWRLFKKISExpressionMammalianMutationwild typePromoterCMVAvailable SinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF1720
Plasmid#144304PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-IGSF9-COMP5AP-AviTag-9xHis
Plasmid#157517PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-FLT1-COMP5AP-AviTag-9xHis
Plasmid#157526PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-FGFR3-COMP5AP-AviTag-9xHis
Plasmid#157360PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-KIR2DL5B-COMP5AP-AviTag-9xHis
Plasmid#157208PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-NCR2-COMP5AP-AviTag-9xHis
Plasmid#157132PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-VSTM4-COMP5AP-AviTag-9xHis
Plasmid#157117PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-IGSF9-Fc(DAPA)-AviTag-6xHis
Plasmid#156953PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertIGSF9 (IGSF9 Human)
TagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available SinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-VSTM4-Fc(DAPA)-AviTag-6xHis
Plasmid#156552PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertVSTM4 (C10orf72 Human)
TagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available SinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-HA PSPC1 Y523F
Plasmid#124373PurposeExpresses PSPC1 Y523F in mammalian cellsDepositorAvailable SinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SFN_14-3-3
Plasmid#109937PurposeProtein expression and purification of SFN_14-3-3DepositorInsertSFN_14-3-3
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-378a-3p
Plasmid#103503PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-378a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-378a-3p target (MIR377 Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-TRE-dCas9-VPR
Plasmid#63800PurposeSP-dCas9-VPR with doxycycline-inducible expressionDepositorInsertdCas9-VPR
UseCRISPR and Synthetic Biology; PiggybacTagsVPR (VP64-p65-Rta)ExpressionMammalianPromoterTREAvailable SinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
SPLICS Mt-ER Short P2A
Plasmid#164108PurposeDetect the short Mitochondria-Endoplasmic reticulum contactDepositorInsertSplit GFP Mt-ER Short P2A
ExpressionMammalianAvailable SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only
