58,349 results

1. TRIM28 gRNA (BRDN0001146162)

   Plasmid

   #76142

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TRIM28

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   TRIM28 (TRIM28 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pN1-OPA1(123Nt)-mScarletI

   Plasmid

   #129343

   Purpose

   Mitochondrial intermembrane space localization

   Depositor

   Yasushi Okada

   Insert

   mScarlet(i) (Addgene 85044)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. MC5R-Tango

   Plasmid

   #66431

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   MC5R (MC5R Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

4. ATX3-13Q

   Plasmid

   #184248

   Purpose

   Recombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track fused with His-Tag and TEV cleavage sequence (N-terminal)

   Depositor

   Sandra Macedo Ribeiro

   Article

   Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111

   Insert

   ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track (ATXN3 Synthetic, Human)
   

   Use

   Tags

   6x His-Tag and TEV cleavage site

   Expression

   Bacterial

   Mutation

   C-terminal codon optimization for protein expres…

   Promoter

   T7 promoter

   Available since

   Aug. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBiex-1 AC30_eGFP

   Plasmid

   #82715

   Purpose

   Expresses a GFP-tagged recombinant adenylyl cyclase.

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

   Insert

   Adenylyl Cyclase V/II (ADCY2, ADCY5 Human)
   

   Use

   Tags

   FLAG purification tag and eGFP

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   T7 Promoter

   Available since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPHR-mCherry-hNCL

   Plasmid

   #181886

   Purpose

   Soluble light-dependent effector protein (nucleolin) for optodroplet formation or recruitment to CIBN localizers

   Depositor

   Karsten Rippe

   Article

   Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

   Insert

   PHR-mCherry-hNCL (NCL Mustard Weed, Human)
   

   Use

   Tags

   PHR-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDH-3xFLAG-hTCAB1-puroR

   Plasmid

   #167459

   Purpose

   3rd-gen lentiviral expression of FLAG-TCAB1 (WDR79) in mammalian cells

   Depositor

   Steven Artandi

   Article

   Chen et al Cell. 2018 Jun 28;174(1):218-230.e13. do

   Insert

   TCAB1 (WRAP53 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. tdTomato-Zyxin-6

   Plasmid

   #58148

   Purpose

   Localization: Focal Adhesions, Excitation: 554, Emission: 581

   Depositor

   Michael Davidson

   Article

   Shaner et al J Cell Sci. 2007 Dec 15;120(Pt 24):4247-

   Insert

   Zyxin (ZYX Human)
   

   Use

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

9. ATP1A1_G4_Q118R_Std_Dual_epegRNA_tevopreQ1

   Plasmid

   #187454

   Purpose

   Coselection for prime editing in human cells. Vector for tandem expression of ATP1A1 Q118R-G4 pegRNA with a user-specified epegRNA. pU6-tevopreq1-GG-acceptor-like plasmid

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G4 Q118R pegRNA (ATP1A1 Human)
   

   Use

   CRISPR; Prime editing

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tandem U6 promoters

   Available since

   July 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. human pirh2-(1-261)

    Plasmid

    #24878

    Depositor

    Cheryl Arrowsmith

    Article

    Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

    Insert

    Pirh2 (RCHY1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pFUW-tetO-BCL6

    Plasmid

    #139816

    Purpose

    doxycycline-inducible expression of Human BCL6 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

    Insert

    BCL6 (BCL6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    T190A, E241G, L314M- please see depositor comments

    Promoter

    TRE-mCMV

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. Crimson/P2A-ICP8 (pSLIK1)

    Plasmid

    #113856

    Purpose

    For Doxycycline-inducible expression of ICP8 synaptase, with red fluorescent protein gene E2-Crimson-P2A-ICP8

    Depositor

    Richard S Myers

    Article

    Valledor et al PLoS One. 2018 Aug 15;13(8):e0200955. do

    Insert

    ICP8
    

    Use

    Lentiviral

    Tags

    P2A/Crimson

    Expression

    Mutation

    Promoter

    Available since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCIneoRL-BCL2 3'UTR

    Plasmid

    #84597

    Purpose

    Renilla luciferase reporter containing BCL2 3' UTR

    Depositor

    Yanan Yang

    Article

    Zhang et al Nat Commun. 2016 Jul 26;7:12231. doi: 10

    Insert

    BCL2 3'UTR (UTS2R Human)
    

    Use

    Luciferase

    Tags

    Renilla Luciferase

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

14. OPTN_Halo_C_allele

    Plasmid

    #178144

    Purpose

    Donor vector for endogenous tagging of human OPTN at the C-terminus with halotag

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Halotag (OPTN Human)
    

    Use

    Donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. TRAV25

    Plasmid

    #237982

    Purpose

    Encodes TRAV25 allele, P2A, and TRBC to generate TCRs via cloning

    Depositor

    Michael Birnbaum

    Insert

    TRBC-P2A-TRAV25 (TRAV25 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    July 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAVLINK-EF1alpha-5' KDM5C

    Plasmid

    #236670

    Purpose

    5' AAVLINK plasmid for KDM5C expression

    Depositor

    Zhonghua Lu

    Insert

    KDM5C (KDM5C Human)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mutation

    Promoter

    EF1alpha

    Available since

    June 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pACEMam2-TRPML1

    Plasmid

    #238303

    Purpose

    Expression of human TRPML1 in mammalian cells

    Depositor

    Pravin Mahajan

    Article

    Judith et al Structure, 2025

    Insert

    TRPML1 (MCOLN1 Human)
    

    Use

    Tags

    8xHis-3xFLAG-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    hybrid CAG promoter

    Available since

    June 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLIB-WDR61

    Plasmid

    #231729

    Purpose

    Protein expression in insect cells

    Depositor

    Jesper Svejstrup

    Article

    Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

    Insert

    WDR61 (SKIC8 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. lentiCRISPRv2-sgCTL

    Plasmid

    #234771

    Purpose

    Non Targeting Control

    Depositor

    Judy Lieberman

    Article

    Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

    Insert

    Non Targeting Control sgRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLJC2-MTHFD1-3xFLAG

    Plasmid

    #217429

    Purpose

    Lentiviral overexpression of human MTHFD1

    Depositor

    Jason Cantor

    Article

    Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

    Insert

    MTHFD1 (MTHFD1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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