59,636 results

1. His6-EGFP-PH-PLCD1

   Plasmid

   #183675

   Purpose

   PtdIns(4,5)P2 biosensor for purification from bacteria

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   PLCD1 (PLCD1 Human)
   

   Tags

   His6-EGFP

   Expression

   Bacterial

   Mutation

   Amino acids 11-140

   Promoter

   T7

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. His-MBP_G4G6 in pRSF

   Plasmid

   #188454

   Purpose

   Express in E. coli human gelsolin segments 4 through 6 with N-terminal 6xHis and MBP tags.

   Depositor

   Roberto Dominguez

   Article

   Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

   Insert

   G4G6 (GSN Human)
   

   Tags

   6xHis-MBP

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. hnRNPA1-FLAG-IRES-eGFP

   Plasmid

   #234619

   Purpose

   Mammalian expression of full-length hnRNPA1 with C-terminal FLAG tag co-expressing with eGFP via IRES sequence

   Depositor

   Tanja Mittag

   Article

   Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

   Insert

   hnRNPA1 (HNRNPA1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   C-terminal FLAG tag, co-expressing with eGFP via …

   Promoter

   CMV

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. TFORF2059

   Plasmid

   #143997

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NKX2-3 (NKX2-3 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRK-Myc-Smurf2

   Plasmid

   #13678

   Depositor

   Ying Zhang

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2001 Jan 30. 9

   Insert

   Smurf2 (SMURF2 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   Feb. 8, 2007

   Availability

   Academic Institutions and Nonprofits only

6. BirA-Myc_N_PAX6

   Plasmid

   #167721

   Purpose

   Expresses PAX6 in mammalian cells

   Depositor

   Markku Varjosalo

   Article

   Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

   Insert

   PAX6 (PAX6 Human)
   

   Tags

   Myc-BirA

   Expression

   Mammalian

   Mutation

   no mutations

   Available Since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Frt-hspB5

   Plasmid

   #63096

   Purpose

   Expression of HSPB5 (small HSP) in mammalian cells

   Depositor

   Harm Kampinga

   Article

   Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

   Insert

   hspb5 (CRYAB Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDEST-CMV mCherry-GFP-LC3B G120A

   Plasmid

   #123235

   Purpose

   Expresses mCherry-EGFP-LC3B G120A in mammalian cells. Negative control for tandem reporter. High expression driven by CMV promoter.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
   

   Tags

   EGFP and mCherry

   Expression

   Mammalian

   Mutation

   Glycine 120 to Alanine

   Promoter

   CMV

   Available Since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIRES-hrGFP II-TET1

   Plasmid

   #83568

   Purpose

   Expresses TET1 in mammalian cells

   Depositor

   Jean-Pierre Issa

   Article

   Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

   Insert

   Tet Methylcytosine Dioxygenase 1 (TET1 Human)
   

   Tags

   3X FLAG tag and hr GFP II

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV5-AML1B

    Plasmid

    #12426

    Purpose

    pCMV5-AML1B is the original plasmid used in Meyers et al 1995. However, please note that the insert sequence has now been identified as AML1C.

    Depositor

    Scott Hiebert

    Article

    Meyers et al Mol Cell Biol. 1995 Apr . 15(4):1974-82.

    Insert

    AML1B (RUNX1 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA4/TO/Strep-HA-ZAK beta

    Plasmid

    #141195

    Purpose

    Expresses Strep-HA-ZAK beta in mammalian cells, can be used to make inducible cell line

    Depositor

    Simon Bekker-Jensen

    Article

    Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20

    Insert

    MAP3K20 isoform 2 (MAP3K20 Human)
    

    Tags

    Strep, HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. tetO-AKT1

    Plasmid

    #170682

    Purpose

    doxycycline-inducible overexpression of AKT1

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Expression

    Mammalian

    Promoter

    TRE promoter, Tet-ON

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1 HA-BACH1

    Plasmid

    #232269

    Purpose

    Expression of N-terminal tagged HA-BACH1 (H. sapiens) in human cell lines.

    Depositor

    Michele Pagano

    Article

    Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

    Insert

    BACH1 (BACH1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. DYRK1A

    Plasmid

    #38913

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    DYRK1A (DYRK1A Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    codon-optimized

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3.1-CCND1

    Plasmid

    #172632

    Purpose

    Expresses untagged cyclin D1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND1 (CCND1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO.1 Rheb1 shRNA #1

    Plasmid

    #26625

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    Rheb1 shRNA (RHEB Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Oct. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pShuttle-FEN1hWT

    Plasmid

    #35027

    Depositor

    Sheila Stewart

    Article

    Saharia et al J Biol Chem. 2010 Aug 27;285(35):27057-6

    Insert

    FEN1 (FEN1 Human)
    

    Use

    Adenoviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

18. VAMP7 HA-delta pMEP4

    Plasmid

    #62958

    Purpose

    full length human VAMP7 with C-terminal HA tag cloned into delta pMEP4

    Depositor

    Paul Luzio

    Article

    Pryor et al Cell. 2008 Sep 5;134(5):817-27. doi: 10.

    Insert

    VAMP7 (VAMP7 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pHMR1A

    Plasmid

    #102957

    Purpose

    gene cloning of MR1A

    Depositor

    Gennaro De Libero, Lucia Mori

    Article

    Lepore et al Nat Commun. 2014 May 15;5:3866. doi: 10.

    Insert

    MR1A cDNA (MR1 Human)
    

    Use

    Cloning

    Mutation

    Stop codon has been removed by HindIII site

    Available Since

    Nov. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. A1-LCD WT

    Plasmid

    #169713

    Purpose

    Bacterial expression of human hnRNPA1-A Low complexity domain including amino acids 186-320

    Depositor

    Tanja Mittag

    Article

    Martin et al Science. 2020 Feb 7;367(6478):694-699. d

    Insert

    hnRNPA1 (HNRNPA1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    hnRNPA1-A Low complexity domain including amino a…

    Promoter

    T7

    Available Since

    June 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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