2,172 results

Showing: 301 - 330 of 2172 results

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th-P2A-Gal4 donor

Plasmid

#65563

Purpose

Intron targeting-mediated Gal4 knockin at the zebrafish th locus.

Depositor

Jiu-lin Du

Article

Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

Insert

th-P2A-Gal4 (th Zebrafish)

Use

Zebrafish injection

Promoter

none

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

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MiniCoopR 2xU6:gRNA pten, mitfa:Cas9

Plasmid

#118845

Purpose

Targets ptena and ptenb and expresses zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Insert

ptena and ptenb (ptena, ptenb Zebrafish)

Use

CRISPR; Tol2

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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ZE26 Stbm-N-6myc-pCS2+

Plasmid

#17068

Depositor

Randall Moon

Article

Park et al Nat Cell Biol. 2002 Jan;4(1):20-5.

Insert

Stbm (vangl2 Zebrafish)

Use

Zebrafish expression

Tags

Myc6

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
Hox D4a

Plasmid

#36469

Depositor

Robert Ho

Article

Prince et al Development. 1998 Feb . 125(3):407-20.

Insert

Hox D4a (hoxd4a Zebrafish)

Use

Riboprobe synthesis

Expression

Bacterial

Mutation

Q100H, A101T, G125C and R189W

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only
ZE45 Dvl-2 pCS2P+

Plasmid

#17086

Depositor

Randall Moon

Article

Waxman et al Development. 2004 Dec. 131(23):5909-21.

Insert

Dvl-2 (dvl2 Zebrafish)

Use

Zebrafish expression

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pCK074 p5E exorh:EGFP

Plasmid

#195951

Purpose

Gateway p5E vector with reporter

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

exorh:EGFP

Use

Other

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pTol2_claudink:Cas9green; erbb2_gRNA171

Plasmid

#199339

Purpose

pDEL142; transgenic construct to express cell-specific Cas9 in oligodendrocytes; ubiquitous erbb2 sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

claudink
Cas9
erbb2 sgRNA

Use

Tol2 destination vector

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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zCREST3:Cas9green; EGF_gRNA

Plasmid

#199333

Purpose

pDEL158; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 EGF sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

zCREST3
Cas9
nrg1 EGF sgRNA

Use

Tol2 destination vector

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pKSII/INSeGFP

Plasmid

#53787

Purpose

1 kb zebrafish insulin promoter driving eGFP

Depositor

Larry Moss

Article

diIorio et al Dev Biol. 2002 Apr 1;244(1):75-84.

Insert

INS promoter/eGFP/pA

Use

Zebrafish

Tags

eGFP

Expression

Bacterial

Promoter

zebrafish insulin/ZIP

Available Since

Jan. 7, 2015

Availability

Academic Institutions and Nonprofits only
pCS2-MT-mitfa

Plasmid

#81231

Purpose

expression of zebrafish mitfa

Depositor

James Lister, David Raible

Article

Lister et al Development. 1999 Sep;126(17):3757-67.

Insert

mitfa (mitfa Zebrafish)

Use

Zebrafish expression

Tags

6 myc tags

Expression

Mammalian

Mutation

contains amino acids 5-412

Promoter

sCMV IE94 promoter

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCS2 Halo-Tev- HSPB7 (zebrafish) WT

Plasmid

#108916

Purpose

Express WT Zebrafish Halo-Tev-HSPB7 in mammalian cells

Depositor

Yimon Aye

Article

Surya et al ACS Chem Biol. 2018 Feb 8. doi: 10.1021/

Insert

Halo-TEV-HSPB7 (hspb7 Zebrafish)

Tags

Halo-TEV

Expression

Mammalian

Promoter

SP6

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pISceI Dusp6:memGFP

Plasmid

#32668

Purpose

Expresses membrane localized EGFP under the Dusp6 promoter

Depositor

Michael Tsang

Article

Wang et al Development. 2011 Jan;138(1):45-54. Epub

Insert

Dusp6 promoter (dusp6 Zebrafish)

Use

Zebrafish transgenesis

Available Since

Nov. 28, 2011

Availability

Academic Institutions and Nonprofits only

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pISceI Dusp6:D2EGFP

Plasmid

#32667

Depositor

Michael Tsang

Article

Molina et al BMC Dev Biol. 2007 Jun 6;7:62.

Insert

Dusp6 promoter (dusp6 Zebrafish)

Use

Zebrafish transgenesis

Tags

EGFP

Available Since

Nov. 28, 2011

Availability

Academic Institutions and Nonprofits only

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pENTR - CaSR-EGFP

Plasmid

#191503

Purpose

A zebrafish CaSR-EGFP construct used for Gateway cloning.

Depositor

Michael Granato

Article

Shoenhard et al Cell Rep. 2022 Dec 6;41(10):111790. doi:

Insert

CaSR-EGFP-SV40

Use

Zebrafish expression

Promoter

none

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+ Integrin beta 1b-mKate

Plasmid

#105975

Purpose

in vitro transcription, Full length zebrafish Integrin beta 1b tagged C terminally with mKate2 using Gateway cloning.

Depositor

Caren Norden

Article

Sidhaye et al Elife. 2017 Apr 4;6. pii: e22689. doi: 1

Insert

Integrinbeta1b

Tags

mKate

Expression

Bacterial

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pPRISM-TagRFP-gcry-eGFP

Plasmid

#117767

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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mpeg:Rab7-stop-polyA; cmlc2:GFP

Plasmid

#213738

Purpose

Expresses mCherry-Rab7 in the macrophage lineage of zebrafish with a green heart transgenesis marker

Depositor

William Talbot

Article

Iyer et al Sci Adv. 2022 Sep 2;8(35):eabp8321. doi:

Insert

mCherry-Rab7

Use

Zebrafish

Promoter

mpeg1.1

Available Since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-rab27a

Plasmid

#109617

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab27a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab27a (rab27a Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pLV_PP1-mCitrine

Plasmid

#172472

Purpose

Mammalian expression of a human codon-optimized version of the optogenetic GPCR parapinopsina (PP1) from zebrafish fused to mCitrine. Also contains an N-terminal prolactin signal sequence.

Depositor

Sean R Collins

Article

Bell et al Nat Commun. 2021 Nov 16;12(1):6148. doi:

Insert

prolactinSS-PP1-mCitrine (parapinopsina Zebrafish)

Use

Lentiviral

Tags

mCitrine and prolactin signal sequence (cleaved p…

Expression

Mammalian

Promoter

EF1alpha

Available Since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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Zf Slit1a

Plasmid

#61279

Purpose

contains zebrafish Slit1a cDNA

Depositor

Chi-Bin Chien, Lara Hutson

Article

Hutson et al Dev Dyn. 2003 Nov;228(3):358-69.

Insert

Slit1a (slit1a Zebrafish)

Use

In vitro transcription

Available Since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only
Zf Slit1b

Plasmid

#61280

Purpose

contains zebrafish Slit1b cDNA

Depositor

Chi-Bin Chien, Lara Hutson

Article

Hutson et al Dev Dyn. 2003 Nov;228(3):358-69.

Insert

Slit1b (slit1b Zebrafish)

Use

Cloning vector

Available Since

Dec. 16, 2014

Availability

Academic Institutions and Nonprofits only
pDEST-Tol2(Chien)-Krt4:Gal4VP16:tdTomato:5xUAS:HyPer:pA

Plasmid

#105643

Purpose

Co-expression of tdTomato and HyPer in differentiated keratinocytes of the periderm layer and ealy on also in the basal layer of the epidermis

Depositor

Sandra Rieger

Article

Lisse et al Proc Natl Acad Sci U S A. 2016 Apr 12;11

Insert

Krt4:Gal4VP16:tdTomato:5xUAS:HyPer:pA

Expression

Mammalian

Available Since

Feb. 2, 2018

Availability

Academic Institutions and Nonprofits only
ZP10 Snail

Plasmid

#16931

Depositor

Randall Moon

Insert

snail (snai1a Zebrafish)

Expression

Bacterial

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
p5E-6xgata2aECEbasp

Plasmid

#132968

Purpose

gata2a endothelial enhancer (x6) and basal promoter in p5E Gateway vector

Depositor

Nathan Lawson

Article

Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

Insert

gata2a endothelial enhancer (x6) with a b-actin basal promoter

Use

Gateway vector

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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p3E-rab27a

Plasmid

#109685

Purpose

Multisite gateway vector for 3' tagging with rab27a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab27a (rab27a Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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Rab27a cds in pGem-T Easy

Plasmid

#80546

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Article

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

rab27a (rab27a Zebrafish)

Available Since

April 20, 2017

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-CDC42

Plasmid

#109531

Purpose

Muscle specific zebrafish expression of mKate2 fused to CDC42

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

CDC42 (cdc42 Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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hsp70:mKate2-NWASP-CA

Plasmid

#105967

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

Insert

NWASP-CA

Tags

mKate2

Expression

Bacterial

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pHis-zfCSF3a

Plasmid

#65612

Purpose

express soluble His-tagged zebrafish CSF3a/Gcsfa in E. coli

Depositor

Petr Bartunek

Article

Stachura et al Blood. 2011 Aug 4;118(5):1274-82. doi: 1

Insert

Csf3a (csf3a Zebrafish)

Tags

(His)6

Expression

Bacterial

Promoter

T7

Available Since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only
pPRISM-gal4-VP16-cmlc2-TagBFP

Plasmid

#117780

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

gal4/VP16

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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