Skip to main content
Addgene
Showing: 501 - 550 of 17678 results

  1. Rtn2b-RHD272-472-GFP

    Plasmid
    #186606
    Purpose
    Encodes human Reticulon 2 isoform B reticulon homology domain aa 272 to 472 fused to GFP
    Depositor
    Gia Voeltz
    Article
    Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6
    Insert
    Human Reticulon 2 isoform B amino acids 272 to 472 (RTN2 Human)
    Use
    Tags
    mNeonGreen
    Expression
    Mammalian
    Mutation
    Met-Gly-Ser-Lys peptide added at N-terminus for c…
    Promoter
    CMV
    Available since
    Feb. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    186606-plasmid-map-sequence-id-369199

    Enlarge

  2. Rtn2b-RHD272-472-mNeon

    Plasmid
    #186605
    Purpose
    Encodes human Reticulon 2 isoform B reticulon homology domain aa 272 to 472 fused to mNeonGreen
    Depositor
    Gia Voeltz
    Article
    Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6
    Insert
    Human Reticulon 2 isoform B amino acids 272 to 472 (RTN2 Human)
    Use
    Tags
    mNeonGreen
    Expression
    Mammalian
    Mutation
    Met-Gly-Ser-Lys peptide added at N-terminus for c…
    Promoter
    CMV
    Available since
    Feb. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    186605-plasmid-map-sequence-id-369207

    Enlarge

  6. pDCAF3-IS5

    Plasmid
    #65220
    Purpose
    Expresses N-demethylases for the degredation of caffeine and related methylxanthines. Can be used to measure caffeine content as described in the publication listed below.
    Depositor
    Jeffrey Barrick
    Article
    Quandt et al ACS Synth Biol. 2013 Jun 21;2(6):301-7.
    Inserts
    ndmA
    ndmB
    ndmC
    ndmD
    gst9
    chlR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Changed start codon from gtg to atg and Changed s…
    Promoter
    BBa_J23100
    Available since
    Jan. 8, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    65220-plasmid-map-sequence-id-134450

    Enlarge

  7. pETM6_Ptrc_BsMdh_BmHps_BmPhi_Pthl_CaThl_CaCtfAB_CaAdc

    Plasmid
    #129105
    Purpose
    Expresses Mdh from Bacillus subtilis and Hps & Phi from Bacillus methanolicus as well as Clostridium acetobutylicum genes required for Acetate-dependent Acetone production
    Depositor
    Eleftherios Terry Papoutsakis
    Article
    Bennett et al Metab Eng. 2018 Jan;45:75-85. doi: 10.10
    Insert
    BsMDH BmHPS BmPHI CaTHL CaCtfAB CaADC
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 11, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    129105-plasmid-map-sequence-id-255085

    Enlarge

  8. pCMV-dCas13-inactive M3M14nes

    Plasmid
    #157855
    Purpose
    Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant
    Depositor
    David Liu
    Article
    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103
    Insert
    dCas13-inactive M3M14nes (METTL3, METTL14 Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    PspCas13b Δ984-1090 H133A; METTL3 D395A
    Promoter
    Available since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    157855-plasmid-map-sequence-id-307628

    Enlarge

  9. pALS2-sfGFP WT

    Plasmid
    #197575
    Purpose
    Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin of replication
    Depositor
    Ryan Mehl
    Article
    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3
    Inserts
    sfGFP WT
    M. alvus Pyl-tRNA (6)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    araC and lpp
    Available since
    June 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    197575-plasmid-map-sequence-id-396574

    Enlarge

  11. pAcBac1-Ma-sfGFP WT

    Plasmid
    #197567
    Purpose
    Expression of sfGFP WT with Methanomethylophilus alvus (Ma) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells
    Depositor
    Ryan Mehl
    Article
    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3
    Inserts
    sfGFP WT
    M. alvus Pyl-tRNA (6) (4x copies)
    Use
    Tags
    V5-His6
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and U6/H1
    Available since
    April 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    197567-plasmid-map-sequence-id-389420

    Enlarge

  12. pALS2-sfGFP 150TAG

    Plasmid
    #197574
    Purpose
    Fluorescence selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-150TAG and M. alvus Pyl-tRNA(6). p15a origin of replication.
    Depositor
    Ryan Mehl
    Article
    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3
    Inserts
    sfGFP 150TAG
    M. alvus Pyl-tRNA (6)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    N150TAG
    Promoter
    araC and lpp
    Available since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    197574-plasmid-map-sequence-id-388126

    Enlarge

  13. pAcBac1-Ma-sfGFP 150TAG

    Plasmid
    #197568
    Purpose
    Expression of sfGFP-150TAG with Methanomethylophilus alvus (Ma) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells
    Depositor
    Ryan Mehl
    Article
    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3
    Inserts
    sfGFP 150TAG
    M. alvus Pyl-tRNA (6) (4x copies)
    Use
    Tags
    V5-His6
    Expression
    Mammalian
    Mutation
    N150TAG
    Promoter
    CMV and U6/H1
    Available since
    March 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    197568-plasmid-map-sequence-id-388231

    Enlarge

  14. Ma-Barnase

    Plasmid
    #197572
    Purpose
    Negative selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses 2xTAG-codon interrupted barnase gene and M. alvus Pyl-tRNA(6). p15a origin of replication.
    Depositor
    Ryan Mehl
    Article
    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3
    Inserts
    Barnase - 2xTAG
    M. alvus Pyl-tRNA (6)
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    3TAG and 45TAG
    Promoter
    araC and lpp
    Available since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    197572-plasmid-map-sequence-id-388086

    Enlarge

  15. Ma-pREP

    Plasmid
    #197571
    Purpose
    Positive selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses TAG-codon interrupted chloramphenicol resistance cassette and M. alvus Pyl-tRNA(6). p15a ori
    Depositor
    Ryan Mehl
    Article
    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3
    Inserts
    Chloramphenicol resistance gene - 111TAG
    T7 polymerase -TAG
    GFPuv
    M. alvus Pyl-tRNA(6)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    111TAG and 8TAG/112TAG
    Promoter
    AraC, T7, cat promoter (constitutive), and lpp (c…
    Available since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    197571-plasmid-map-sequence-id-389424

    Enlarge

  16. pAcBac1-Ma-Acridone-RS1 (A7)

    Plasmid
    #197566
    Purpose
    Expression of Methanomethylophilus alvus (Ma) Acridone RS1 (A7) tRNA synthetase/Pyl-tRNA pair for the encoding of Acridone at TAG codons in HEK293 cells
    Depositor
    Ryan Mehl
    Article
    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3
    Inserts
    M. alvus PylRS - Acd RS1 (A7)
    M. alvus Pyl-tRNA (6) (4x copies)
    Use
    Tags
    None
    Expression
    Mammalian
    Mutation
    N166A, V168G, W239C
    Promoter
    CMV and U6/H1
    Available since
    March 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    197566-plasmid-map-sequence-id-388228

    Enlarge

  26. pBS NL4-3 envFS eGFP 5'CMV Δtat

    Plasmid
    #101347
    Purpose
    tat ATG->ACG (silent in vpr reading frame), nt 78 mutated T->G to change Tyr to stop codon, nt 116 mutated T->C to disrupt Met, 5'LTR replaced with CMV-R-U5 from pALPS for tat-independent transcription in HEK293E cells.
    Depositor
    Jeremy Luban
    Article
    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:
    Insert
    NL4-3
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    delta Tat
    Promoter
    Available since
    March 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    101347-plasmid-map-sequence-id-194257

    Enlarge

  31. pComb3HSS

    Plasmid
    #64133
    Purpose
    2nd generation plasmid for phage display on modified geneIII, contains stuffer fragment
    Depositor
    Carlos Barbas
    Article
    Barbas et al Methods, A companion to Methods in Enzym
    Inserts
    SS Stuffer
    Light Chain Stuffer
    Heavy Chain Stuffer
    Use
    Phage display
    Tags
    geneIII
    Expression
    Bacterial
    Mutation
    Promoter
    lacZ
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    64133-plasmid-map-sequence-id-119143

    Enlarge

  33. pAJE-Ma pylRS nitroY/haloY-F5

    Plasmid
    #225684
    Purpose
    Expresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control during Ma Pyl tRNA-synthetase validation.
    Depositor
    Ryan Mehl
    Inserts
    M. alvus PylRS 3-NY/HaloY F5
    M. alvus Pyl-tRNA(6)
    Use
    Methanomethylophilus alvus
    Tags
    Expression
    Mutation
    CTG125CTT, N166A, V168S, A223C, W239R
    Promoter
    GlnS and Lpp
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    225684-plasmid-map-sequence-id-450701

    Enlarge

  34. pAraH6HATT

    Plasmid
    #63901
    Purpose
    For expression of soluble scFv/Fab fragments in bacteria from genes selected using the pComb3H system, contains TT Fab (light and heavy chains) against tetanus toxin, used as expression control.
    Depositor
    Carlos Barbas
    Article
    Barbas et al Methods, A companion to Methods in Enzym
    Insert
    TT Fab
    Use
    Tags
    6xHis and HA tag
    Expression
    Bacterial
    Mutation
    Promoter
    araBAD
    Available since
    Feb. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    63901-plasmid-map-sequence-id-136457

    Enlarge

  35. pComb3HTT

    Plasmid
    #63900
    Purpose
    Backbone of pComb3H containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control.
    Depositor
    Carlos Barbas
    Article
    Barbas et al Methods, A companion to Methods in Enzym
    Insert
    TT Fab
    Use
    Phage display
    Tags
    geneIII
    Expression
    Bacterial
    Mutation
    Promoter
    lacZ
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63900-plasmid-map-sequence-id-119142

    Enlarge

  40. SBC015898

    Plasmid
    #226285
    Purpose
    Expresses PhCFAT, ObEGS1, and ObEOMT1 from trc promoter. Biosynthesis of methylated phenylpropenes from monolignol precursors.
    Depositor
    Rainer Breitling
    Article
    Chua et al Metab Eng Commun. 2024 May 15;18:e00237.
    Inserts
    Coniferyl alcohol acyltransferase
    Eugenol synthase 1
    Eugenol O-methyltransferase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    226285-plasmid-map-sequence-id-451912

    Enlarge

  45. pAS_4xMx PylT C41CA_FLAG_Mx PylRS YA

    Plasmid
    #140014
    Purpose
    Plasmid with 4xMx PylT C41CA cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021
    Insert
    Mx1201 PylRS YA (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Y126A, Y197F
    Promoter
    EF1
    Available since
    May 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    140014-plasmid-map-sequence-id-297743

    Enlarge

  46. pCMV-dCas13-inactive M3nls

    Plasmid
    #157854
    Purpose
    Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant
    Depositor
    David Liu
    Article
    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103
    Insert
    dCas13-inactive dCas13-inactive M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    PspCas13b Δ984-1090 H133A; METTL3 D395A
    Promoter
    Available since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    157854-plasmid-map-sequence-id-307627

    Enlarge

  47. MTST5

    Plasmid
    #86602
    Purpose
    Custom designed synthetic DNA fragment lacking homology to natural sequences for preparation of internal standards for RNA-seq in metatranscriptomics.
    Depositor
    Mary Ann Moran
    Article
    Satinsky et al Methods Enzymol. 2013;531:237-50. doi: 1
    Insert
    MTST5
    Use
    Internal standard reverse transcription
    Tags
    Expression
    Mutation
    Promoter
    T7
    Available since
    Sept. 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    86602-plasmid-map-sequence-id-193516

    Enlarge

  48. MTST6

    Plasmid
    #86603
    Purpose
    Custom designed synthetic DNA fragment lacking homology to natural sequences for preparation of internal standards for RNA-seq in metatranscriptomics.
    Depositor
    Mary Ann Moran
    Article
    Satinsky et al Methods Enzymol. 2013;531:237-50. doi: 1
    Insert
    MTST6
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Sept. 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    86603-plasmid-map-sequence-id-193515

    Enlarge

Showing: 501 - 550 of 17678 results