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We narrowed to 558 results for: Tore

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Showing: 501 - 550 of 558 results

  2. pZH517

    Plasmid
    #102670
    Purpose
    Tetracycline inducible expression of GFPmut2 using autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Expression
    Bacterial
    Available Since
    Dec. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    102670-plasmid-map-sequence-id-199582

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  3. pZH519

    Plasmid
    #102672
    Purpose
    Tetracycline inducible expression of GFPmut2 using autoregulation; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Expression
    Bacterial
    Available Since
    Dec. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    102672-plasmid-map-sequence-id-199600

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  4. pZH518

    Plasmid
    #102671
    Purpose
    Tetracycline inducible expression of GFPmut2 using autoregulation; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Expression
    Bacterial
    Available Since
    Dec. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    102671-plasmid-map-sequence-id-199394

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  5. pMOD8A-RGR-r10

    Plasmid
    #74370
    Purpose
    This plasmid constitutively expresses gRNA-r10 from an AHD1 promoter using the RGR design. Integrates in the W303 HIS locus and restores HIS function.
    Depositor
    Eric Klavins
    Article
    Gander et al bioRxiv 041871
    Insert
    RGR-r10
    Use
    CRISPR and Synthetic Biology
    Expression
    Yeast
    Promoter
    ADH1
    Available Since
    April 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    74370-plasmid-map-sequence-id-141329

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  6. pZH509

    Plasmid
    #102664
    Purpose
    Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Expression
    Bacterial
    Available Since
    Dec. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    102664-plasmid-map-sequence-id-199389

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  7. pMOD8A-iRGR-r11

    Plasmid
    #74369
    Purpose
    This plasmid constitutively expresses gRNA-r11 from an AHD1 promoter using the insulated RGR design. Integrates in the W303 HIS locus and restores HIS function.
    Depositor
    Eric Klavins
    Article
    Gander et al bioRxiv 041871
    Insert
    RGR-r11
    Use
    CRISPR and Synthetic Biology
    Expression
    Yeast
    Promoter
    AHD1
    Available Since
    April 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    74369-plasmid-map-sequence-id-141324

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  9. pZH512

    Plasmid
    #102666
    Purpose
    Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Expression
    Bacterial
    Available Since
    Dec. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    102666-plasmid-map-sequence-id-199387

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  11. pZH511

    Plasmid
    #102665
    Purpose
    Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Expression
    Bacterial
    Available Since
    Dec. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    102665-plasmid-map-sequence-id-199381

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  19. pCMV6-AC EJ7-GFP

    Plasmid
    #113617
    Purpose
    Reporter for canonical-NHEJ. Reporter for end joining between two double-strand breaks, induced with the 7a and 7b sgRNAs with CAS9. EJ between the distal ends w/o indel mutations restores GFP
    Depositor
    Jeremy Stark
    Article
    Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:
    Insert
    EJ7-GFP variant of EGFP
    Expression
    Mammalian
    Available Since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    113617-plasmid-map-sequence-id-218254

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  20. pCMV6-AC 4-μHOM

    Plasmid
    #113619
    Purpose
    Reporter for Alt-EJ, variant of EJ7-GFP. Double strand breaks are induced with the 7a & 7h, or 7a & 7i sgRNAs, with CAS9. EJ using 4 nt of microhomology causes a deletion mutation and restores GFP
    Depositor
    Jeremy Stark
    Article
    Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:
    Insert
    4-μHOM variant of EGFP
    Expression
    Mammalian
    Available Since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    113619-plasmid-map-sequence-id-218256

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  26. GST-prRDH-CTS

    Plasmid
    #86069
    Purpose
    A bacterial expression plasmid encoding photoreceptor retinol dehydrogenase ciliary targeting signal with N-terminus GST-tag.
    Depositor
    Lei Lu
    Article
    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393
    Insert
    Retinol dehydrogenase (RDH8 Bovine)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    the insert is 297-312 of RDH8 (Bos taurus)
    Promoter
    tac
    Available Since
    May 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    86069-plasmid-map-sequence-id-177979

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  27. OpenLoopControl

    Plasmid
    #59895
    Purpose
    synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Does not contain the target containing Vamp3 3′UTR
    Depositor
    Georg Seelig
    Article
    Strovas et al ACS Synth Biol. 2014 May 16;3(5):324-31.
    Insert
    mCherry with intron containing the mouse mir-124–3
    Tags
    PEST
    Expression
    Mammalian
    Promoter
    doxycycline (Dox)-inducible promoter (CMV/TO)
    Available Since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    59895-plasmid-map-sequence-id-108253

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  34. sgFFL

    Plasmid
    #59877
    Purpose
    synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene.
    Depositor
    Georg Seelig
    Article
    Strovas et al ACS Synth Biol. 2014 May 16;3(5):324-31.
    Insert
    mCherry with intron containing the mouse mir-124–3
    Tags
    PEST and VAMP 3 UTR
    Expression
    Mammalian
    Promoter
    doxycycline (Dox)-inducible promoter (CMV/TO)
    Available Since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    59877-plasmid-map-sequence-id-108244

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  39. sgFFL_3x_siRNA

    Plasmid
    #59893
    Purpose
    synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with three siRNA-like sites
    Depositor
    Georg Seelig
    Article
    Strovas et al ACS Synth Biol. 2014 May 16;3(5):324-31.
    Insert
    mCherry with intron containing the mouse mir-124–3
    Tags
    PEST and VAMP 3 UTR
    Expression
    Mammalian
    Mutation
    VAMP 3 UTR--has three siRNA-like targets (fully c…
    Promoter
    doxycycline (Dox)-inducible promoter (CMV/TO)
    Available Since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    59893-plasmid-map-sequence-id-108250

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  41. sgFFL_d23

    Plasmid
    #59892
    Purpose
    synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with two seed sites mutated
    Depositor
    Georg Seelig
    Article
    Strovas et al ACS Synth Biol. 2014 May 16;3(5):324-31.
    Insert
    mCherry with intron containing the mouse mir-124–3
    Tags
    PEST and VAMP 3 UTR
    Expression
    Mammalian
    Mutation
    VAMP 3 UTR--3rd and fourth seed site removed
    Promoter
    doxycycline (Dox)-inducible promoter (CMV/TO)
    Available Since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    59892-plasmid-map-sequence-id-108249

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  42. sgFFL_d3

    Plasmid
    #59891
    Purpose
    synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with one seed site mutated.
    Depositor
    Georg Seelig
    Article
    Strovas et al ACS Synth Biol. 2014 May 16;3(5):324-31.
    Insert
    mCherry with intron containing the mouse mir-124–3
    Tags
    PEST and VAMP 3 UTR
    Expression
    Mammalian
    Mutation
    VAMP 3 UTR--3rd seed site removed
    Promoter
    doxycycline (Dox)-inducible promoter (CMV/TO)
    Available Since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    59891-plasmid-map-sequence-id-108247

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  47. PEA1-Nuclease

    Plasmid
    #176528
    Purpose
    Delivers all prime editing nuclease components in a single plasmid
    Depositor
    Paul Thomas
    Article
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Insert
    CbH-Cas9-RT, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    GC to CA point mutation in SpCas9 to restore nucl…
    Promoter
    CbH for Cas9, hU6 for gRNAs
    Available Since
    Dec. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176528-plasmid-map-sequence-id-350557

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  48. sgFFL_7x_Bulge

    Plasmid
    #59894
    Purpose
    synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with seven bulge target sites
    Depositor
    Georg Seelig
    Article
    Strovas et al ACS Synth Biol. 2014 May 16;3(5):324-31.
    Insert
    mCherry with intron containing the mouse mir-124–3
    Tags
    PEST and VAMP 3 UTR
    Expression
    Mammalian
    Mutation
    VAMP 3 UTR--has seven bulge targets (fully comple…
    Promoter
    doxycycline (Dox)-inducible promoter (CMV/TO)
    Available Since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    59894-plasmid-map-sequence-id-108252

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Showing: 501 - 550 of 558 results