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pGEX-human p53-(1-393)

Plasmid

#24860

Depositor

Cheryl Arrowsmith

Article

Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

Insert

p53 (TP53 Human)

Tags

GST

Expression

Bacterial

Available Since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-AURKB

Plasmid

#23682

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

AURKB (AURKB Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pCIG-VP16 RARa [TJ#389]

Plasmid

#16287

Depositor

Thomas Jessell

Article

Novitch et al Neuron. 2003 Sep 25. 40(1):81-95.

Insert

RAR alpha (RARA Human)

Tags

VP16

Expression

Mammalian

Available Since

Feb. 4, 2009

Availability

Academic Institutions and Nonprofits only
pET3a-Ub-K63R

Plasmid

#18898

Depositor

Cecile Pickart

Article

Raasi et al Methods Mol Biol. 2005;301:47-55. doi: 1

Insert

Ubiquitin-K63R (UBC Human)

Expression

Bacterial

Mutation

Lysine 63 is replaced with a Argnine residue

Available Since

Sept. 10, 2008

Availability

Academic Institutions and Nonprofits only

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Jag1-HA pIRES

Plasmid

#17336

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

Jagged1 (Jag1 Rat)

Tags

HA X3

Expression

Mammalian

Mutation

A silent C->G mutation at 3237

Available Since

April 24, 2008

Availability

Academic Institutions and Nonprofits only

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pBabe-Puro-IKBalpha-wt

Plasmid

#15290

Depositor

William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

inhibitor of Kappa B alpha (NFKBIA Human)

Use

Retroviral

Expression

Mammalian

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only
pLTR-G

Plasmid

#17532

Depositor

Jakob Reiser

Article

Reiser et al Proc Natl Acad Sci U S A. 1996 Dec 24. 9

Insert

VSV-G glycoprotein (Indiana strain)

Use

Lentiviral

Expression

Mammalian

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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pCI HA NEDD4 DD

Plasmid

#26999

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

NEDD4 (NEDD4 Human)

Tags

HA

Expression

Mammalian

Mutation

C867A

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pHAGE-KRAS

Plasmid

#116755

Purpose

Lentiviral expression of KRAS

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

KRAS (KRAS Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCR-Flag-IKKalpha-KM

Plasmid

#15468

Depositor

Hiroyasu Nakano

Article

Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

Insert

IkB kinase alpha-KM (Chuk Mouse)

Tags

Flag

Expression

Mammalian

Mutation

changed Lysine at 44 to Alanine

Available Since

Oct. 10, 2007

Availability

Academic Institutions and Nonprofits only
pLenti-EmGFP-LATS2/1 KD

Plasmid

#52085

Purpose

Lentiviral RNAi vector for knockdown of LATS2 and LATS1. Co-expresses EmGFP as reporter

Depositor

Yutaka Hata

Article

Yang et al Mol Cell Biol. 2014 Feb 18.

Insert

LATS2 and LATS1 miRNAs (LATS1, LATS2 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

May 13, 2014

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-SARS-CoV-2-orf10-2xStrep-IRES-Puro

Plasmid

#141394

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-orf10 (ORF10 SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR207 SARS-CoV-2 NSP1

Plasmid

#141255

Purpose

Gateway-compatible Entry vector, with insert of NSP1 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP1 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pHAGE-N-FLAG-HA-TBK1

Plasmid

#131791

Purpose

Lentiviral vector expressing WT TBK1

Depositor

Alicia Pickrell

Article

Sarraf et al Cell Rep. 2019 Oct 1;29(1):225-235.e5. d

Insert

TBK1 WT (TBK1 Human)

Use

Lentiviral

Tags

N-FLAG-HA

Expression

Mammalian

Mutation

WT full length

Promoter

CMV

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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V5-APEX2-LMNA

Plasmid

#129275

Purpose

Expression of APEX2 at nuclear lamina

Depositor

Alice Ting

Article

Fazal et al Cell. 2019 Jun 14. pii: S0092-8674(19)30

Insert

V5-APEX2-LMNA

Use

Lentiviral

Expression

Mammalian

Promoter

CMV promoter

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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SeeSaw Reporter (SSR2.0)

Plasmid

#50393

Purpose

SSR2.0 has two target sites in opposite orientation for generate only one cycle of repairing after I-SceI endonuclease expression.

Depositor

Pablo Huertas Sánchez

Article

Gomez-Cabello et al PLoS One. 2013 Oct 14;8(10):e77206. doi:

Insert

SSR2.0

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pBABE HA Chk2 WT

Plasmid

#41901

Depositor

Stephen Elledge

Article

Matsuoka et al Science. 2007 May 25;316(5828):1160-6.

Insert

CHK2 (CHEK2 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only

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pCXLE-gw

Plasmid

#37626

Purpose

Non-integrating (episomal) expression vector with Gateway cassette

Depositor

Shinya Yamanaka

Article

Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

Type

Empty backbone

Expression

Mammalian

Available Since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only

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MSCV-Myc-IRES-RFP

Plasmid

#35395

Depositor

Martine Roussel

Article

Kawauchi et al Cancer Cell. 2012 Feb 14;21(2):168-80.

Inserts

Myc (Myc Mouse)
RFP

Use

Retroviral

Expression

Mammalian

Available Since

March 5, 2012

Availability

Academic Institutions and Nonprofits only
pAAV-hPDGFRA-GFP

Plasmid

#22919

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

human platelet derived growth factor receptor alpha polypeptide promoter driving GFP (PDGFRA Human)

Use

AAV

Expression

Mammalian

Available Since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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