We narrowed to 24,551 results for: p

1. MIG-eIF4A1 Q195E

   Plasmid

   #70044

   Purpose

   Expression of eIF4A1 Q195E mutant in mammalian cells

   Depositor

   Hans-Guido Wendel

   Article

   Wolfe et al Nature. 2014 Sep 4;513(7516):65-70. doi:

   Insert

   eIF4A1 (Eif4a1 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   Q195E

   Promoter

   CMV

   Available Since

   Aug. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pDEST-N2H-F1-empty vector

   Plasmid

   #125551

   Purpose

   N2H assay vector with nanoluc fragment1 (empty control, no Gateway cloning site, expressing nanoluc fragment1)

   Depositor

   Marc Vidal

   Article

   Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:

   Type

   Empty backbone

   Use

   In vitro expression

   Tags

   NanoLuc fragment1 alone without any attached prot…

   Expression

   Mammalian and Yeast

   Promoter

   synthetic promoter containing yeast, mammalian, a…

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pαH-S-GSAS-P1-like

   Plasmid

   #173789

   Purpose

   Mammalian expression of SARS-CoV-2 Spike protein P-1-like variant (K417N)

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Science. 2021 Jun 24. pii: science.abi62

   Insert

   Spike S-GSAS- P-1-like variant (K417N) (S Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

   Expression

   Mammalian

   Mutation

   Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

   Available Since

   Aug. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDONR223 SARS-CoV2 TEV-NSP5

   Plasmid

   #154404

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   NSP5 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector, with insert of n…

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   July 1, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pDONR223 SARS-CoV-2 NSP8_nostop

   Plasmid

   #149311

   Purpose

   Gateway-compatible Entry vector, with insert of NSP8 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   NSP8 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   May 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pDONR223 SARS-CoV2 TEV-S

   Plasmid

   #154415

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   S (S SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector, with insert of s…

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   Sept. 2, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. FLINC-AKAR1(TA)-CAAX

   Plasmid

   #87706

   Purpose

   Negative-control mutant of FLINC-AKAR1-CAAX.

   Depositor

   Jin Zhang

   Article

   Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

   Insert

   FLINC-AKAR1(TA)-CAAX
   

   Tags

   6xHis, C-terminal targeting sequence from Kras, T…

   Expression

   Mammalian

   Mutation

   Target Thr residue in substrate domain mutated to…

   Promoter

   CMV

   Available Since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGL3-sgACSL3.C-Cas9-P2A-Puro

   Plasmid

   #129412

   Purpose

   Encoding Cas9 and sgACSL3.C for CRISPR/Cas9 mediated HDR tagging of endogenous human ACSL3 C-terminus

   Depositor

   Elina Ikonen

   Article

   Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

   Insert

   SpCas9 and sgACSL3.C (ACSL3 Human)
   

   Use

   CRISPR

   Tags

   P2A-Puro

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pXPR_BRD111

   Plasmid

   #78166

   Purpose

   Expresses Cas9 with the pLX311 backbone

   Depositor

   William Hahn

   Article

   Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

   Insert

   Cas9
   

   Use

   Lentiviral

   Tags

   3xFLAG and NLS

   Available Since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

10. VN-PTEN

    Plasmid

    #89331

    Purpose

    expresses hPTEN fused to the N-terminal part of Venus

    Depositor

    Tiago Outeiro

    Article

    Marciniak et al J Exp Med. 2017 Aug 7;214(8):2257-2269.

    Insert

    PTEN1 (PTEN Human)
    

    Tags

    N-terminal part of Venus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDONR223 SARS-CoV2 TEV-NSP1

    Plasmid

    #154400

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    NSP1 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector, with insert of n…

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    July 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pDECKO_GFP_AAVS1

    Plasmid

    #141211

    Purpose

    pDECKO_GFP_AAVS1_1801. Targeting, non-essential locus control

    Depositor

    Rory Johnson

    Article

    Esposito et al Cell Genom. 2022 Aug 22;2(9):100171. doi

    Insert

    gRNA
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGL3-Basic-ACSL3-sfGFP(C) HDR template

    Plasmid

    #129413

    Purpose

    HDR tempalte for tagging of endogenous human ACSL3 C-terminus with sfGFP

    Depositor

    Elina Ikonen

    Article

    Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

    Insert

    ACSL3 HDR template (ACSL3 Human)
    

    Use

    CRISPR and TALEN; Endogenous tagging hdr template

    Tags

    sfGFP

    Expression

    Mammalian

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDECKO_mCherry_AAVS1_1801

    Plasmid

    #159090

    Purpose

    targeting, non-essential locus control

    Depositor

    Rory Johnson

    Article

    Esposito et al Cell Genom. 2022 Aug 22;2(9):100171. doi

    Insert

    gRNA
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDONR223 SARS-CoV-2 ORF10wu_nostop

    Plasmid

    #152986

    Purpose

    Gateway-compatible Entry vector, with insert of ORF10wu gene's CDS from SARS-CoV-2 genome definition in Wu et al, Nature 2020

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    ORF10wu
    

    Use

    Gateway-compatible entry vector

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    May 27, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pDONR223 SARS-CoV2 TEV-NSP6

    Plasmid

    #154405

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    NSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector, with insert of n…

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    Sept. 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pDONR223 SARS-CoV2 TEV-NSP13

    Plasmid

    #154411

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    NSP13 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector, with insert of n…

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    July 2, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pDONR221 SARS-CoV-2 S-frag5_nostop

    Plasmid

    #153177

    Purpose

    Gateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

    Depositor

    Fritz Roth, Mikko Taipale

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    S-frag5
    

    Use

    Gateway-compatible entry vector

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    May 27, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pDONR223 SARS-CoV2 TEV-N

    Plasmid

    #154424

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    N (N SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector, with insert of n…

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    Sept. 2, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pDONR223 SARS-CoV-2 ORF9Bwu_nostop

    Plasmid

    #149328

    Purpose

    Gateway-compatible Entry vector, with insert of ORF9Bwu gene's CDS from SARS-CoV-2 genome definition in Wu et al, Nature 2020. Does not contain stop codon.

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    ORF9Bwu
    

    Use

    Gateway-compatible entry vector

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    May 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge