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We narrowed to 59,752 results for: Gene

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Showing: 3331 - 3360 of 59752 results

  4. pCMV-T7-SpG-P2A-EGFP (RTW4177)

    Plasmid
    #139988
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9 variant named SpG with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139988-plasmid-map-sequence-id-275338

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  14. pCRISPomyces-1

    Plasmid
    #61736
    Purpose
    Streptomyces expression of codon-optimized Cas9, tracrRNA, and custom crRNA
    Depositor
    Huimin Zhao
    Article
    Cobb et al ACS Synth Biol. 2014 Dec 8.
    Inserts
    sSpCas9
    tracrRNA
    crRNA cassette
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    BbsI-flanked lacZ cassette inserted in place of s…
    Promoter
    gapdhp(EL), rpsLp(CF), and rpsLp(XC)-BbsI
    Available Since
    Jan. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    61736-plasmid-map-sequence-id-256988

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  18. pDG462

    Plasmid
    #100903
    Purpose
    SpCas9n (D10A Nickase mutant) with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A mutant converts to Nickase
    Promoter
    CBh and U6
    Available Since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100903-plasmid-map-sequence-id-196607

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  19. pSicoR-Ef1a-mCh

    Plasmid
    #31847
    Purpose
    Cre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression.
    Depositor
    Bruce Conklin
    Article
    Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107
    Type
    Empty backbone
    Use
    Cre/Lox, Lentiviral, and RNAi
    Expression
    Mammalian
    Promoter
    U6 for shRNA; Ef1alpha for mCherry
    Available Since
    Jan. 3, 2012
    Availability
    Academic Institutions and Nonprofits only
    31847-plasmid-map-sequence-id-35883

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  20. pTriEx-mVenus-PA-Rac1

    Plasmid
    #22007
    Purpose
    Expression of photoactivatable Rac1
    Depositor
    Klaus Hahn
    Article
    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.
    Insert
    PA-Rac1 (RAC1 Avena sativa (oat), Human)
    Tags
    6X His and mVenus
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    LOV(Leu404-Leu546), Rac1 starts at I4 and contain…
    Promoter
    CMV, p10
    Available Since
    Sept. 29, 2009
    Availability
    Academic Institutions and Nonprofits only

  21. pTriEx-PA-Rac1

    Plasmid
    #22024
    Purpose
    Expression of photoactivatable Rac1
    Depositor
    Klaus Hahn
    Article
    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.
    Insert
    PA-Rac1 (RAC1 Avena sativa (oat), Human)
    Tags
    6X His
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    Rac1 starts at I4 and contains mutations Q61L, E9…
    Promoter
    CMV, p10
    Available Since
    Sept. 29, 2009
    Availability
    Academic Institutions and Nonprofits only

  30. MS2_SC2_S_Omicron

    Plasmid
    #180229
    Purpose
    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Omicron SARS-CoV-2 S gene (Amino Acid 319-869)
    Depositor
    Paul Freemont
    Inserts
    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    Expression
    Bacterial
    Mutation
    Omicron (Amino Acid 319-869)
    Promoter
    T7
    Available Since
    Jan. 28, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    180229-plasmid-map-sequence-id-352514

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Showing: 3331 - 3360 of 59752 results