We narrowed to 23,099 results for: CRISPR

1. p201H Cas9

   Plasmid

   #59176

   Purpose

   Cas9 driven by double 35S, hygromycin resistance for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

   Depositor

   Wayne Parrott

   Article

   Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

   Inserts

   Cas9
   hph
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   2x35S and StUbi-3P

   Available Since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRH2521

   Plasmid

   #84380

   Purpose

   Expression of sgRNA from a imyc promoter (Pimyc)

   Depositor

   Robert Husson

   Article

   Singh et al Nucleic Acids Res. 2016 Jul 12. pii: gkw

   Insert

   sgRNA cloning site
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAGE-TO-nmdCas9-3XmCherry

   Plasmid

   #64110

   Purpose

   Expression of Nm dCas9-3XmCherry in mammalian cells

   Depositor

   Thoru Pederson

   Article

   Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

   Insert

   Nm dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3XmCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TO

   Available Since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYL-kasOp-FnCas12a3

   Plasmid

   #177875

   Purpose

   Expresses Streptomyces codon optimized FnCas12a. Used to modify genome of Streptomyces.

   Depositor

   Yunzi Luo

   Article

   Zhang et al Front Bioeng Biotechnol. 2020 Jun 30;8:7

   Insert

   Streptomyces codon optimized the FnCas12a mutant EP16
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   kasOp*

   Available Since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLH-spsgRNA2

   Plasmid

   #64114

   Purpose

   Vector for expression of Sp sgRNA2 in mammalian cells

   Depositor

   Thoru Pederson

   Article

   Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

   Insert

   Sp sgRNA2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGG>nls-hCas9-nls-GFP

   Plasmid

   #99141

   Purpose

   Cas9-GFP fusion protein under the regulation of chicken beta-actin promoter

   Depositor

   Marianne Bronner

   Article

   Gandhi et al Dev Biol. 2017 Dec 1;432(1):86-97. doi:

   Insert

   Cas9-GFP
   

   Use

   CRISPR

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Chicken beta actin (CAG)

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRU295

   Plasmid

   #167694

   Purpose

   Final destination vector for single Gateway LR reaction

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   pEC1.2::Cas9i
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. BPK2660

   Plasmid

   #70709

   Purpose

   Human expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHAGE-TO-nmdCas9-3XGFP

   Plasmid

   #64109

   Purpose

   Expression of Nm dCas9-3XGFP in mammalian cells

   Depositor

   Thoru Pederson

   Article

   Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

   Insert

   Nm dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3XsfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TO

   Available Since

   April 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330GFP-hU6-gRNA-hSpCas9

    Plasmid

    #128385

    Purpose

    GFP positive gRNA/hSpCas9 construct

    Depositor

    Budd A. Tucker

    Article

    Giacalone et al Curr Protoc Stem Cell Biol. 2018 Feb 28;

    Insert

    GFP, gRNA and hSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDN-Sth1Cas9

    Plasmid

    #221386

    Purpose

    Cas9 under control of AHT inducible TetR on integrating plasmid (KanR) using L5 integrase and attP for site-specific integration into attB

    Depositor

    Deborah Hung

    Article

    Neo et al J Bacteriol. 2024 Mar 21;206(3):e0033523

    Insert

    Sth1 Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLH-nmsgRNA1.1

    Plasmid

    #64115

    Purpose

    Vector for expression of Nm sgRNA1.1 in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    Nm sgRNA1.1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV hUbC-dCas9 KRAB-T2A-GFP

    Plasmid

    #67620

    Purpose

    Co-expressed human optimized S. pyogenese dCas9 fused to KRAB repressor domain and GFP

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Insert

    humanized dead Cas9 KRAB T2A GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mutation

    D10A and H840A

    Promoter

    Available Since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. gRNA Cloning Vector Bbs I ver. 2

    Plasmid

    #85586

    Purpose

    An empty sgRNA expression vector. sgRNA sequence can be cloned into its Bbs I site. Its gRNA scaffold has efficient ver. 2 structure.

    Depositor

    Hodaka Fujii

    Article

    Fujita et al Sci Rep. 2016 Jul 28;6:30485. doi: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Dec. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSL0283 (pTnsABC)

    Plasmid

    #130633

    Purpose

    Expresses V. cholerae CAST TnsA, TnsB, and TnsC from a T7 promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchTnsA, VchTnsB, VchTnsC
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFC330

    Plasmid

    #87842

    Purpose

    AMA1 plasmid with Aspergillus optimized Cas9 and pyrG selection marker

    Depositor

    Uffe Mortensen

    Article

    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

    Inserts

    Cas9
    pyrG
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    Aspergillus nidulans tef1 promoter and native pro…

    Available Since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV hUbC-dCas9 VP64-T2A-GFP

    Plasmid

    #53192

    Purpose

    Co-expresses human optimized S. pyogenes dCas9-VP64 and GFP

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Insert

    humanized dead Cas9 VP64 T2A GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D10A and H840A

    Promoter

    hUbC

    Available Since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. P4 cosmid

    Plasmid

    #196335

    Purpose

    P4 cosmid with CRISPR-Cas9 system

    Depositor

    Baojun Wang

    Article

    Fa-Arun et al ACS Synth Biol. 2023 Feb 17;12(2):596-60

    Insert

    genes from phage P4, CRISPR-Cas9 system
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRU294

    Plasmid

    #167689

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pEC1.2::Cas9i
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pC0052-REPAIR non-targeting guide clone into pC0043

    Plasmid

    #103868

    Purpose

    Non-targeting guide RNA for use with PspCas13b. Useful as a control for RNA editing and RNA knockdown experiments.

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    non-targeting guide RNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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