Addgene

We narrowed to 75,578 results for: ELL

Showing: 2241 - 2260 of 75578 results

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HP138-puro

Plasmid

#134246

Purpose

Cas9 in a transposon array under a Tet inducible promoter. For making inducible Cas9 cell lines. Puro selection.

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

Contains Cas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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FU-tet-o-hOct4

Plasmid

#19778

Depositor

Konrad Hochedlinger

Article

Maherali et al Cell Stem Cell. 2008 Sep 11. 3(3):340-5.

Insert

Oct4 (POU5F1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro Xlone-eGFP

Plasmid

#136936

Purpose

donor plasmid for inducible transgene expression in human cells

Depositor

Xiaoping Bao

Article

Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.

Insert

all-in-one tet-on system

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

TRE3GS

Available since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

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INS-2A-luciferase-2A-tdT

Plasmid

#159348

Purpose

Human INS targeting vector for knockin of 2A separated luciferase and tdTomato. Targeted cells will be puromycin resistant.

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

Insert

Luciferase-2A-tdTomato

Use

Ins locus knockin donor vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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FUGW-d2GFP-200

Plasmid

#79602

Purpose

FUGW lentiviral vector with constiutively expressed destabilized GFP (d2GFP) regulated by 5 miR-200 family binding sites

Depositor

Jeffrey Rosen

Article

Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

Insert

d2GFP + miR-200 family targets

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pMBA328

Plasmid

#214748

Purpose

Medium copy glmS-nadE plasmid containing mCherry and no antibiotic resistance gene (ARG)

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23119-RBS(22821)-mcherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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mVenus-P115-C1

Plasmid

#112931

Purpose

p115RhoGEF tagged with mVenus for detection of Ga13 activity

Depositor

Dorus Gadella

Article

Mastop et al PLoS One. 2018 Mar 5;13(3):e0193705. doi

Insert

p115 RhoGEF

Use

Tags

mVenus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1aL-mCherry-W

Plasmid

#126703

Purpose

Constitutive, ubiquitous mCherry expression.

Depositor

Darrell Kotton

Article

McCauley et al Cell Stem Cell. 2017 Jun 1;20(6):844-857

Insert

mCherry

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Ef1aL

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pMP71-Flt3L

Plasmid

#174600

Purpose

Secretes the extracellular domain of murine Flt3L

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

secretes extracellular domain of mouse Flt3-L

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-oct4

Plasmid

#20323

Depositor

Rudolf Jaenisch

Article

Brambrink et al Cell Stem Cell. 2008 Feb 7. 2(2):151-9.

Insert

oct4 (Pou5f1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2009

Availability

Academic Institutions and Nonprofits only

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FSW-HRP-V5-SLITRK3

Plasmid

#82539

Purpose

Lentiviral vector for expressing HRP-SLITRK3 in neurons

Depositor

Alice Ting

Article

Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

Insert

SLITRK3 (SLITRK3 Human)

Use

Lentiviral

Tags

HRP and V5

Expression

Mammalian

Mutation

Promoter

Human Synapsin

Available since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only

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FSW-HRP-V5-NLGN2

Plasmid

#82545

Purpose

Overexpression vector for V5-NLGN2 in neurons

Depositor

Alice Ting

Article

Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

Insert

NLGN2 (Nlgn2 Rat)

Use

Lentiviral

Tags

HRP and V5

Expression

Mammalian

Mutation

Promoter

Human Synapsin

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pPyCAG-tagBFP-3xNLS-IRES-Hph

Plasmid

#183608

Purpose

Mammalian expression vector for tagBFP-3xNLS from the CAG promoter. Confers hygromycin B resistance.

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Insert

tagBFP-3xNLS

Use

Tags

3xNLS

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCyPet-Rac1(Q61L)

Plasmid

#22783

Depositor

Klaus Hahn

Article

Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

Insert

Rac1 (Q61L) (RAC1 A. victoria, Human)

Use

Tags

CyPet

Expression

Mammalian

Mutation

Rac1 Q61L.

Promoter

Available since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
Fra-1

Plasmid

#187906

Purpose

Expresses Fra-1 in transfected cells

Depositor

Jennifer Mitchell

Article

Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

Insert

Fra1 (Fosl1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 5HT2A_NB2_1

Plasmid

#127690

Purpose

Plasmid contains a positive allosteric modulating nanobody the binds to the cytoplasmic surface of 5HT2A.

Depositor

Justin English, Bryan Roth

Article

English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

Insert

5HT2A_NB2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pP2A_NLS_sfGFP

Plasmid

#63709

Purpose

Expressing P2A_NLS_sfGFP with homologous arm of Nanog.

Depositor

Stanley Qi

Article

Yu et al Cell Stem Cell. 2015 Feb 5;16(2):142-7.

Insert

Nanog homologous arm, p2A, NLS, sfGFP, Nanog homologous arm

Use

Just a donor dna with nanog homologous arm for kn…

Tags

sfGFP

Expression

Mutation

Promoter

Available since

April 27, 2015

Availability

Academic Institutions and Nonprofits only

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pDisplay sHRPb-KDEL

Plasmid

#73150

Purpose

Small fragment of split HRP targeted for expression in the lumen of the endoplasmic reticulum (ER). CMV promoter.

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

sHRPb-KDEL

Use

Tags

V5 tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pTREX GPR68_NB2_2

Plasmid

#127691

Purpose

Plasmid contains a nanobody that directly interacts with the cytoplasmic surface of GPR68.

Depositor

Justin English, Bryan Roth

Article

English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

Insert

GPR68_NB2_2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pDisplay sHRPa-KDEL

Plasmid

#73149

Purpose

Large fragment of split HRP targeted for expression in the lumen of the endoplasmic reticulum (ER). CMV promoter.

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

sHRPa-KDEL

Use

Tags

FLAG tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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