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Showing: 3391 - 3420 of 22701 results

  1. pAJ1

    Plasmid
    #138379
    Purpose
    pEcRNAP6 derivative encoding Escherichia coli core RNA polymerase subunits: RpoA, RpoB, RpoC(His)10, RpoZ
    Depositor
    Seth Darst
    Article
    Cartagena et al Proc Natl Acad Sci U S A. 2019 Sep 17;11
    Inserts
    RpoA (rpoA E. coli)
    RpoB (rpoB E. coli)
    RpoC (rpoC E. coli)
    rpoZ (rpoZ E. coli)
    Tags
    His10
    Expression
    Bacterial
    Mutation
    Clamp toe deletion: Δ(Tyr144-Lys179) ::(G144-S145…
    Promoter
    T7
    Available Since
    April 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    138379-plasmid-map-sequence-id-275999

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  2. pVZ_PcoaT_SQE1

    Plasmid
    #103860
    Purpose
    Heterologous, cobalt-inducible expression of SQE1 from A. thaliana in Synechocystis sp. PCC6803
    Depositor
    Ilka Axmann
    Article
    Loeschcke et al PLoS One. 2017 Dec 27;12(12):e0189816. d
    Insert
    squalene epoxidase 1 (XF1 Mustard Weed)
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    inserted gene is codon optimized. 2nd amino acid …
    Promoter
    PcoaT (from Synechocystis sp. PCC 6803)
    Available Since
    Jan. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    103860-plasmid-map-sequence-id-202305

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  3. pCA528 IST1 L328D,L355A (316-366)(C-Cys)

    Plasmid
    #193037
    Purpose
    Bacterial expression plasmid for IST1 (316-366) with C-terminal Cysteine for fluorescence polarization binding assays. Contains L328D and L355A mutations that diminishes binding to MIT domains.
    Depositor
    Wesley Sundquist
    Article
    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75
    Insert
    IST1 (IST1 Human)
    Tags
    6xHis-Sumo
    Expression
    Bacterial
    Mutation
    L328D, L355A, Residues 316-366, Non-native C-term…
    Promoter
    T7
    Available Since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    193037-plasmid-map-sequence-id-379006

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  12. pLVX-2xFLAG-2xSTREP-CCND1(T286I)-IRES-mCherry

    Plasmid
    #172638
    Purpose
    Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1(T286I) and mCherry in mammalian cells
    Depositor
    Michele Pagano
    Article
    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:
    Insert
    CCND1 (CCND1 Human)
    Use
    Lentiviral
    Tags
    2xFLAG-2xSTREP
    Expression
    Mammalian
    Mutation
    Thr286Ile
    Promoter
    CMV
    Available Since
    Aug. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    172638-plasmid-map-sequence-id-340165

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  18. pBABE-2xFLAG-2xSTREP-CCND1(T286A)-Puro

    Plasmid
    #172633
    Purpose
    Retroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D1(T286A) and a puromycin resistance marker in mammalian cells
    Depositor
    Michele Pagano
    Article
    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:
    Insert
    CCND1 (CCND1 Human)
    Use
    Retroviral
    Tags
    2xFLAG-2xSTREP
    Expression
    Mammalian
    Mutation
    Thr286Ala
    Available Since
    Aug. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    172633-plasmid-map-sequence-id-340219

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  20. pLVX-2xFLAG-2xSTREP-CCND1(P287L)-IRES-mCherry

    Plasmid
    #172636
    Purpose
    Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1(P287L) and mCherry in mammalian cells
    Depositor
    Michele Pagano
    Article
    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:
    Insert
    CCND1 (CCND1 Human)
    Use
    Lentiviral
    Tags
    2xFLAG-2xSTREP
    Expression
    Mammalian
    Mutation
    Pro287Leu
    Promoter
    CMV
    Available Since
    Aug. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    172636-plasmid-map-sequence-id-340185

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  24. pVZ_PcoaT_SQE1_THAS1

    Plasmid
    #103859
    Purpose
    Heterologous, cobalt-inducible expression of SQE1 and THAS1 from A. thaliana in Synechocystis sp. PCC6803
    Depositor
    Ilka Axmann
    Article
    Loeschcke et al PLoS One. 2017 Dec 27;12(12):e0189816. d
    Insert
    thalianol synthase 1 (THAS1 Mustard Weed)
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    inserted genes are codon optimized. 2nd amino aci…
    Promoter
    PcoaT (from Synechocystis sp. PCC 6803)
    Available Since
    July 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    103859-plasmid-map-sequence-id-217087

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  25. pVZ_PcoaT_SQE1_MRN1

    Plasmid
    #103858
    Purpose
    Heterologous, cobalt-inducible expression of SQE1 and MRN1 from A. thaliana in Synechocystis sp. PCC6803
    Depositor
    Ilka Axmann
    Article
    Loeschcke et al PLoS One. 2017 Dec 27;12(12):e0189816. d
    Insert
    marneral synthase 1 (MRN1 Mustard Weed)
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    inserted genes are codon optimized2nd amino acid …
    Promoter
    PcoaT (from Synechocystis sp. PCC 6803)
    Available Since
    Feb. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    103858-plasmid-map-sequence-id-202616

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  26. pVZ_PcoaT_SQE1_CAS1

    Plasmid
    #103856
    Purpose
    Heterologous, cobalt-inducible expression of SQE1 and CAS1 from A. thaliana in Synechocystis sp. PCC6803
    Depositor
    Ilka Axmann
    Article
    Loeschcke et al PLoS One. 2017 Dec 27;12(12):e0189816. d
    Insert
    cycloartenol synthase 1 (CAS1 Mustard Weed)
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    inserted genes are codon optimized. 2nd amino aci…
    Promoter
    PcoaT (from Synechocystis sp. PCC 6803)
    Available Since
    Jan. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    103856-plasmid-map-sequence-id-202299

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  28. pDCAF3-IS5

    Plasmid
    #65220
    Purpose
    Expresses N-demethylases for the degredation of caffeine and related methylxanthines. Can be used to measure caffeine content as described in the publication listed below.
    Depositor
    Jeffrey Barrick
    Article
    Quandt et al ACS Synth Biol. 2013 Jun 21;2(6):301-7.
    Inserts
    ndmA
    ndmB
    ndmC
    ndmD
    gst9
    chlR
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    Changed start codon from gtg to atg and Changed s…
    Promoter
    BBa_J23100
    Available Since
    Jan. 8, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    65220-plasmid-map-sequence-id-134450

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  30. pDGB1alpha2_KanR_BastaR_Rb7_GFP

    Plasmid
    #186427
    Purpose
    optimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar) with fluorescent marker (eGFP)
    Depositor
    Lukas Fischer
    Article
    Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007
    Inserts
    KanR
    BastaR
    Rb7
    eGFP
    Use
    Synthetic Biology; Binary vector for escherichia …
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    CaMV 35S and Pnos
    Available Since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    186427-plasmid-map-sequence-id-369354

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Showing: 3391 - 3420 of 22701 results