We narrowed to 11,551 results for: NSI

1. NPC1-CFP P692S

   Plasmid

   #58457

   Purpose

   expression of NPC1-CFP sterol sensing domain mutant

   Depositor

   Matthew Scott

   Article

   Ko et al Mol Biol Cell. 2001 Mar;12(3):601-14.

   Insert

   NPC1 (Npc1 Mouse)
   

   Use

   Tags

   ECFP and His6

   Expression

   Mammalian

   Mutation

   P692S sterol sensing domain mutant

   Promoter

   CMV

   Available Since

   Oct. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

2. WNV NS2B-NS3 protease (based on PDB 2YOL, catalytically active); aliases: West Nile Virus NS2B-NS3 fusion protein

   Plasmid

   #204798

   Purpose

   Bacterial expression of a fusion of West Nile NS2B-NS3 proteins

   Depositor

   Frank von Delft, ASAP Discovery

   Insert

   West Nile NS2B-NS3 fusion
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 17, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pSW609 - N001

   Plasmid

   #115993

   Purpose

   intermediate vector for GreenGate cloning method, contains modules from A-F

   Depositor

   Sebastien Wolf

   Article

   Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

   Insert

   H-A adapter:pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
   

   Use

   Unspecified

   Tags

   HDEL and SP(ER)

   Expression

   Mutation

   Promoter

   Available Since

   April 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTRE2-Hyg-omMx1

   Plasmid

   #52420

   Purpose

   Inducible expression (Tet-Off system) of the rainbow trout antiviral Mx1 protein

   Depositor

   Bertrand Collet

   Article

   Lester et al J Virol Methods. 2012 Jun;182(1-2):1-8.

   Insert

   Oncorhynchus mykiss interferon-induced GTP-binding protein Mx1 (mx1)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE2

   Available Since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pZH521

   Plasmid

   #102674

   Purpose

   Tetracycline inducible expression of GFPmut2 with constitutive TetR; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

   Depositor

   Zach Hensel

   Article

   Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

   Insert

   GFPmut2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. cacnb1b (rat) in pMT2 vector

   Plasmid

   #107423

   Purpose

   Expresses Calcium channel beta1b auxillary subunit in mammalian cells.

   Depositor

   Annette Dolphin

   Article

   Page et al J Neurosci. 1997 Feb 15;17(4):1330-8.

   Insert

   calcium channel beta1b auxillary subunit
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ad MLP

   Available Since

   April 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pYFAC-riboB

   Plasmid

   #168975

   Purpose

   Filamentous fungi vector with AMA1; 2µ replicon; URA3; AmpR ; riboB

   Depositor

   Yit Heng Chooi

   Article

   Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d

   Type

   Empty backbone

   Use

   Synthetic Biology; Expression in aspergillus nidu…

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. MAC_C_SFXN1

   Plasmid

   #111676

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

   Insert

   SFXN1 (SFXN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMTTH+eGFP-bromo1

   Plasmid

   #157805

   Purpose

   Expression of synthetic protein composed of eGFP and BRD4 bromodomain #1

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   eGFP-bromo1
   

   Use

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   pTAC

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Flag Snail 2SA

    Plasmid

    #16219

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

    Insert

    Snail (SNAI1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    2SA: S97A, S101A

    Promoter

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pZH517

    Plasmid

    #102670

    Purpose

    Tetracycline inducible expression of GFPmut2 using autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

    Depositor

    Zach Hensel

    Article

    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

    Insert

    GFPmut2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAB082h

    Plasmid

    #79240

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    Ppro1 rpoZ-Cry1Ac-d23C
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ppro1

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pENTR-mOvol1

    Plasmid

    #120359

    Purpose

    Gateway entry vector containing mouse Ovol1.

    Depositor

    Knut Woltjen

    Article

    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

    Insert

    Ovol1 (Ovol1 Mouse)
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tet-on and CBh

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJW4.16

    Plasmid

    #62871

    Purpose

    Bacterial expression of MBP-MBK-2

    Depositor

    Geraldine Seydoux

    Article

    Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

    Insert

    MBP-MBK-2
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Lsg-LMNA-1 + 2

    Plasmid

    #199278

    Purpose

    nicking sgRNA to induce LMNA (c.1824C > T) mutation using PE3

    Depositor

    Ting Zhou

    Article

    Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

    Insert

    LMNA nick
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRK5-mFzd1-1D4

    Plasmid

    #42263

    Depositor

    Chris Garcia, Jeremy Nathans

    Article

    Yu et al Development. 2012 Dec 1;139(23):4383-94.

    Insert

    Frizzled 1 (Fzd1 Mouse)
    

    Use

    Tags

    1D4

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 28, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pPnos (GB0072)

    Plasmid

    #68166

    Purpose

    Provides the Agrobacterium tumefaciens Nopaline synthase promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Nos promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCA-G-intron-G

    Plasmid

    #40027

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    GFP exon 1
    GFP exon 2
    beta-globin intron
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    insertion of a loxP site, nucleotides 1-274, and …

    Promoter

    CAG (chicken beta globin promoter and CMV enhance…

    Available Since

    Oct. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGENE:EFF-1::mCherry::V5

    Plasmid

    #133020

    Purpose

    EFF-1 fused to mCherry fluorescent protein cloned into the pGene vector for inducible expression in mammalian cells

    Depositor

    Benjamin Podbilewicz

    Article

    Perez-Vargas et al Cell. 2014 Apr 10;157(2):407-419. doi: 1

    Insert

    EFF-1::mCherry::V5
    

    Use

    Tags

    V5; mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pETCON-TNF

    Plasmid

    #67748

    Purpose

    Tumor Necrosis Factor Alpha Yeast Display Plasmid

    Depositor

    Timothy Whitehead

    Article

    Kowalsky et al J Biol Chem. 2015 Oct 30;290(44):26457-7

    Insert

    TNF-Alpha
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. MAC_N_TOM22

    Plasmid

    #111697

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    TOM22 (TOMM22 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCDNA3.1+ HA-hAO7

    Plasmid

    #137840

    Purpose

    N-terminal tagged human AO7 (aa 2-439) for expression in mammalian cells

    Depositor

    Allan Weissman

    Article

    Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

    Insert

    AO7 (RNF25 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. UAS->Bxb1.STOP>SNAPf>CLIPf>Halo2

    Plasmid

    #87644

    Purpose

    For creating drosophila transgenics expressing UAS->Bxb1.STOP>SNAPf>CLIPf>Halo2

    Depositor

    Gregory Jefferis

    Article

    Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

    Insert

    myr::SNAPf>myr::CLIPf>myr::Halo2
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDGB3_alpha1

    Plasmid

    #68228

    Purpose

    GoldenBraid level 1 Alpha plant expression vector.

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    empty vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMN-011

    Plasmid

    #195415

    Purpose

    AAVS1 Integratable mCherry Reporter for synZiFTR ZF3

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    4X ZF3 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. MAC_N_RAB9A

    Plasmid

    #111693

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    RAB9A (RAB9A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. EK0302 SFFV-mCherry-SG(Bdnf.s2)SG-EGFP (FLP-IN)

    Plasmid

    #191179

    Purpose

    Negative control sensor in human cells.

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:Bdnf.s2:EGFP (negative control)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMZ-314

    Plasmid

    #195459

    Purpose

    Lentiviral Integratable, ABA-inducible expression of synZiFTR: ZF1-p65

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    pSFFV - ABI1 - ZF1 - 2A - p65 - PYL1
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJAG98-mTurquoise2-Nuf2

    Plasmid

    #80760

    Purpose

    Express Nuf2 N-terminally labeled with mTurquoise2

    Depositor

    Daniel Needleman

    Article

    Yoo et al Elife. 2018 Jul 25;7. pii: 36392. doi: 1

    Insert

    Nuf2 (NUF2 Human)
    

    Use

    Retroviral

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDsRed (GB0100)

    Plasmid

    #68202

    Purpose

    Provides the CDS of DsRed as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    DsRed coding region
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pEVOL-MmAcKRS1-PylTUUA

    Plasmid

    #182537

    Purpose

    N‐acetyl lysyl‐tRNA synthetase with cognate tRNA for genetic code expansion with ochre suppression

    Depositor

    Wenshe Liu

    Article

    Wu et al Chembiochem. 2012 Jul 9;13(10):1405-8. d

    Insert

    PylTUUA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ProK

    Available Since

    April 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMSCV-Myc-Caspase-4_W267N

    Plasmid

    #214311

    Purpose

    Expression of gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    CASP4 (CASP4 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    W267N

    Promoter

    MESV

    Available Since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pZH519

    Plasmid

    #102672

    Purpose

    Tetracycline inducible expression of GFPmut2 using autoregulation; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

    Depositor

    Zach Hensel

    Article

    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

    Insert

    GFPmut2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. NmeCas9_3XNLS_pMCSG7

    Plasmid

    #120078

    Purpose

    Bacterial expression of wtNmeCas9 with 3X NLS

    Depositor

    Erik Sontheimer

    Article

    Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

    Insert

    NmeCas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. BK beta1

    Plasmid

    #113565

    Purpose

    mouse BK beta1 subunit in mammalian expression vector

    Depositor

    Robert Brenner

    Article

    Wang et al J Gen Physiol. 2006 Dec;128(6):731-44. d

    Insert

    Kcnmb1 (Kcnmb1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pZH522

    Plasmid

    #102675

    Purpose

    Tetracycline inducible expression of GFPmut2 with constitutive TetR; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

    Depositor

    Zach Hensel

    Article

    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

    Insert

    GFPmut2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. wt HA-dynamin 1 pUHD10-3

    Plasmid

    #34688

    Depositor

    Sandra Schmid

    Article

    Damke et al J Cell Biol. 1995 Oct;131(1):69-80.

    Insert

    dynamin 1 (DNM1L Human)
    

    Use

    Tags

    HA (variant)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

38. pSW178 - pXPP

    Plasmid

    #115987

    Purpose

    entry vector for GreenGate cloning method, promoter

    Depositor

    Alexis Maizel

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    pXPP
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pORE303N_CEN12RRFuzzy3MYB

    Plasmid

    #194441

    Purpose

    CRISPR, Cas9 driven by double 35S, nptII for plant selection, knockout: CEN12, RR, and Fuzzy3MYB

    Depositor

    Chung-Jui Tsai

    Article

    Ortega et al New Phytol. 2022 Nov 16. doi: 10.1111/np

    Insert

    gRNA array targeting CEN1, RR, Fuzzy3MYB
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    MtU6

    Available Since

    Jan. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pRK5-mFzd6-1D4

    Plasmid

    #42268

    Depositor

    Chris Garcia, Jeremy Nathans

    Article

    Yu et al Development. 2012 Dec 1;139(23):4383-94.

    Insert

    Frizzled 6 (Fzd6 Mouse)
    

    Use

    Tags

    1D4

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 28, 2013

    Availability

    Academic Institutions and Nonprofits only