We narrowed to 12,028 results for: LEA
-
Plasmid#182932PurposeFor expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP11 fragmentDepositorTypeEmpty backboneTags3C-cleavable split GFP 11, FLAG tag, and HA signa…ExpressionMammalianPromoterCMVAvailable SinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only
-
P2-ECFP-pA (Construct 6)
Plasmid#55198PurposeSynthetic promoter responsive to gRNA2DepositorInsertmCerulean
UseSynthetic BiologyExpressionMammalianPromoterMinimal Ade MLPAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NG-ABE8e(V106W)
Plasmid#198533PurposeExpresses human codon-optimized SpCas9-NG-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-NG-ABE8e(V106W)-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NG-ABE8e(V106W)
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianPromoterEFSAvailable SinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Aurora A
Plasmid#198402PurposeExpression of EGFP-tagged Aurora A in mammalian cellsDepositorInsertAurora A
TagsEGFPExpressionMammalianAvailable SinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn GFP-Cre
Plasmid#175381Purposeexpresses GFP-Cre under the control of a human synapsin promoterDepositorInsertGFP-Cre recombinase
UseAAVTagsCre is fused to GFP. GFP is fused to additional n…Promoterhuman synapsinAvailable SinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Flag-UAP56 WT
Plasmid#110125PurposeExpresses Flag-tagged human UAP56 (DDX39B)DepositorAvailable SinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET His10 MBP Asn10 TEV LIC cloning vector (2CT-10)
Plasmid#55209DepositorTypeEmpty backboneTags10xAsn, 10xHis, MPB, and TEV cleavage siteExpressionBacterialPromoterT7Available SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
p15a-AsCas12f-blaZ
Plasmid#171609PurposeAsCas12f1-based E. coli genome editingDepositorInsertAsCas12f1
ExpressionBacterialAvailable SinceDec. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-sgTel
Plasmid#122659PurposeExpresses sgRNA for telomere repeats with fluorescent indicator BFP and puromycin selection markerDepositorInsertBFP
UseCRISPR and LentiviralExpressionMammalianAvailable SinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
emiRFP670-cGAS
Plasmid#231896PurposeExpression of cyclic GMP/AMP synthase (cGAS), as a nuclear rupture markerDepositorAvailable SinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-eGFP
Plasmid#221710PurposeExpression of eGFP in zebrafish embryos.DepositorInserteGFP
UseZebrafish expressionPromoterCMV IE94Available SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
371-GFP
Plasmid#97030PurposeHis6-GFP-tev-yORFDepositorTypeEmpty backboneTagsGFP fusion, His6 tag, and TEV cleavage siteExpressionBacterialPromoterT7Available SinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAdSh.U6.gRNAS1
Plasmid#58252PurposeU6 promoter-driven single guide RNA complementary to the human AAVS1 locusDepositorInsertU6.gRNAS1 cassette
UseAdenoviral and CRISPRExpressionMammalianAvailable SinceSept. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HLA-cMyc-EcopT1R1
Plasmid#113962Purposemammalian expression plasmid for c-myc-tagged E. coli codon-optimized human T1R1 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chainDepositorInsertT1R1 (TAS1R1 Human)
TagsSignal/leader sequence from HLA class I histocomp…ExpressionMammalianMutationtruncate N-terminal 24 residuesAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc3-Mdm2 p60
Plasmid#52059Purposeexpression of truncated Mdm2 in mammalian cellsDepositorInsertMdm2 p60 (truncated) (MDM2 Human)
Tagsmyc3ExpressionMammalianMutationp60 (truncated at D367, DVDP motif)PromoterCMVAvailable SinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
AviTag-SpyTag003-MBP
Plasmid#184227PurposeExpresses AviTag and SpyTag003 linked to maltose binding protein in the bacterial cytoplasm. AviTag enables site-specific biotinylation by BirA. SpyTag003 enables reaction with SpyCatcher003.DepositorInsertAviTag-SpyTag003-MBP
TagsAviTag, His6, SpyTag003, and Thrombin cleavage si…ExpressionBacterialPromoterT7Available SinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2 sgNRF2-3
Plasmid#186838Purposeknock out NRF2 in mammalian cellsDepositorInsertNrf2 (Nuclear factor-erythroid factor 2-related factor 2) (NFE2L2 Human)
UseCRISPR and LentiviralAvailable SinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-His6-Ubc9
Plasmid#133909PurposeBacterial expression of His6-Ubc9DepositorInsertSUMO-conjugating enzyme UBC9 (UBE2I Human)
TagsThrombin-cleavable His6-tagExpressionBacterialPromoterT7Available SinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVH-EF1a-GFP-P2A-OCT4
Plasmid#130692PurposeLentiviral plasmids encoding human OCT4 (POU5f1) along with GFP linked via P2A.DepositorInserthuman OCT4 (POU5F1 Human)
UseLentiviralAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET30b-5M SrtA with TEV site
Plasmid#86962PurposeExpresses calcium-dependent Sortase A with a TEV cleavage site proximal to the C-terminal histidine tagDepositorInsertS.aureus SrtA 5M
Tags6x Histidine tag and TEV siteExpressionBacterialMutationP94R, D160N, D165A, K190E, K196TPromoterT7Available SinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only