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pCRISPRyl_A08

Plasmid

#84610

Purpose

Introduces DSB at A08 locus in Y. lipolytica

Depositor

Ian Wheeldon

Publication

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Promoter

UAS1B8-TEF(136)

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLH-sgRNA1

Plasmid

#75388

Purpose

sgRNA1

Depositor

Thoru Pederson

Publication

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

sgRNA1

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

U6

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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lenti-mTagBFP2

Plasmid

#216126

Purpose

Lentiviral vector for expressing mTagBFP2.

Depositor

Stefan Kubicek

Publication

Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

Insert

EF1a-mTagBFP2-WPRE

Use

Lentiviral

Expression

Mammalian

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgSLC7A11/xCT-1

Plasmid

#161818

Purpose

knock out SLC7A11/xCT in mammalian cells

Depositor

Boyi Gan

Publication

Liu et al Nat Cell Biol. 2020 Apr;22(4):476-486. d

Insert

SLC7A11 solute carrier family 7 member 11 xCT (SLC7A11 Human)

Use

CRISPR and Lentiviral

Available since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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PTEN shRNA

Plasmid

#86645

Purpose

expression of shRNA targeting PTEN

Depositor

Jaime Modiano

Publication

Lin et al Mol Cancer. 2007 Feb 14;6:16.

Insert

PTEN (PTEN Human)

Use

RNAi

Expression

Mammalian

Promoter

H1

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRVL-2

Plasmid

#104580

Purpose

Contains mouse IgG2c CH1/CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Full Fc-mediated immune effector functions

Depositor

Daniel Christ

Publication

Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi

Insert

Mouse IgG2c CH1/CH2/CH3

Expression

Mammalian

Promoter

CMV

Available since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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RCAS(A) mdnLef1en (CT#1144)

Plasmid

#14022

Depositor

Cliff Tabin

Insert

Lef1 (Lef1 Mouse)

Use

Retroviral; Avian expression

Mutation

Dominant negative Lef1, engrailed fusion

Available since

Feb. 16, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pET-PfuX7

Plasmid

#182364

Purpose

Expresses PfuX7 DNA polymerase

Depositor

Morten Norholm

Publication

Norholm et al BMC Biotechnol. 2010 Mar 16;10:21. doi:

Insert

PfuX7 DNA polymerase

Tags

His-tag

Expression

Bacterial

Mutation

V93Q

Promoter

T7

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBS-GGAC-ATGC

Plasmid

#60949

Purpose

Drosophila, backbone for donor plasmid assembly. CRISPR knock-in.

Depositor

Frank Schnorrer

Publication

Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

Type

Empty backbone

Use

CRISPR

Available since

Nov. 19, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGPD2

Plasmid

#43972

Depositor

Hal Alper

Publication

Crook et al Nucleic Acids Res. 2011 Aug;39(14):e92.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Promoter

GPD

Available since

April 9, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

6 Articles

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pcDNA3-J chain

Plasmid

#145146

Purpose

Mammalian expression plasmid for J chain of polymeric antibody classes

Depositor

Erik Procko

Publication

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

immunoglobulin J chain (JCHAIN Human)

Expression

Mammalian

Mutation

Codon-optimized for human cell expression.

Promoter

CMV

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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CAG::PSAML141F,Y115F:5HT3HC-IRES-GFP

Plasmid

#32476

Purpose

Expresses PSAM-5HT3 HC (L141F,Y115F) neuronal activator, with IRES-EGFP marker

Depositor

Scott Sternson

Publication

Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

Insert

PSAM Y115F,L141F-5HT3 HC

Tags

IRES-GFP

Expression

Mammalian

Promoter

CAG

Available since

April 10, 2012

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-dCas9-VPR-H2B-mCherry (CRISPRa)

Plasmid

#175572

Purpose

Inactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activation

Depositor

Joseph Brzezinski

Type

Empty backbone

Use

CRISPR

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pX330S-7

Plasmid

#58783

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Publication

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pHR_TRE3GS-->mCherry_PGK-->BFP

Plasmid

#162231

Purpose

Lentiviral vector for the expression of TRE3GS-->mCherry_PGK-->BFP in mammalian cells

Depositor

Wendell Lim

Publication

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

mCherry and BFP

Use

Lentiviral

Expression

Mammalian

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCDNA3.1(+)-NEMOf

Plasmid

#189932

Purpose

Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

Depositor

Yubin Zhou

Publication

Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

Insert

NEMOf

Expression

Mammalian

Promoter

CMV

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCEV-G1-Ph

Plasmid

#46814

Purpose

TEF1 and PGK1 promoter controlled expression cassettes with phleomycin resistance for yeast transformation

Depositor

Lars Nielsen, Claudia Vickers

Publication

Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

Type

Empty backbone

Tags

FLAG and c-myc

Expression

Yeast

Promoter

TEF1 and PGK1

Available since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pENN226_CRISPRcharm

Plasmid

#220834

Purpose

CRISPRcharm expression in mammalian cells

Depositor

Jonathan Weissman

Publication

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

CRISPRcharm

Use

CRISPR

Tags

P2A-TagBFP

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMA7-SacB

Plasmid

#79968

Purpose

TM-MAGE strain

Depositor

Morten Sommer

Publication

Lennen et al Nucleic Acids Res. 2016 Feb 29;44(4):e36

Inserts

Lambda Red recombinase beta subunit
DNA adenine methylase
Levansucrase

Use

Synthetic Biology

Expression

Bacterial

Promoter

pBAD

Available since

Sept. 2, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Hela kinase-dead MLCK-GFP

Plasmid

#46317

Depositor

Anne Bresnick

Publication

Dulyaninova et al J Cell Sci. 2004 Mar 15;117(Pt 8):1481-9

Insert

HeLa long myosin light chain kinase-dead (MYLK Human)

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

E1626K

Promoter

CMV

Available since

July 18, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles