4,176 results

1. pmEGFP-C1

   Plasmid

   #36412

   Depositor

   Benjamin Glick

   Type

   Empty backbone

   Tags

   mEGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcdna nesprin TS

   Plasmid

   #68127

   Purpose

   nesprin tension sensor

   Depositor

   Daniel Conway

   Article

   Arsenovic et al Biophys J. 2016 Jan 5;110(1):34-43. doi:

   Insert

   mouse nesprin mini 2G (Syne2 Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. PGKneolox2DTA.2

   Plasmid

   #13449

   Depositor

   Philippe Soriano

   Insert

   PGKneolox2DTA
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Available Since

   Nov. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-tdPP7-3xmCherry

   Plasmid

   #74926

   Purpose

   Expresses tdPP7-3xmCherry

   Depositor

   Marvin Tanenbaum

   Article

   Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

   Insert

   PP7-3xmCherry
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   April 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMK289 (mAID-mClover-NeoR)

   Plasmid

   #72827

   Purpose

   C-terminal tagging with mAID-mClover using CRISPR/Cas9

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   mAID-mClover-Neo
   

   Use

   CRISPR

   Tags

   mAID-mClover

   Expression

   Mammalian

   Available Since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-FLAG-KD LKB1

   Plasmid

   #8591

   Depositor

   Lewis Cantley

   Article

   Shaw et al Proc Natl Acad Sci U S A 2004 Mar 9;101(

   Insert

   LKB1 (STK11 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   K78I: lysine 78 mutated to isoleucine; kinase dead

   Available Since

   July 18, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-Cas9-His

   Plasmid

   #98158

   Purpose

   Purification of Cas9 protein

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Commun. 2017 Jan 18;8:14261. doi: 10

   Insert

   NLS-Cas9-NLS
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKI1.1R

   Plasmid

   #85808

   Purpose

   CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.

   Depositor

   Tetsuya Higashiyama

   Article

   Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

   Insert

   human-codon-optimized SpCas9
   

   Use

   CRISPR

   Tags

   FLAG and NLS

   Expression

   Plant

   Promoter

   AtRPS5A

   Available Since

   Jan. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. lentiGuide-Hygro-eGFP

   Plasmid

   #99375

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A eGFP from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Inserts

   S. pyogenes sgRNA cassette
   Hygro-P2A-eGFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1a and hU6

   Available Since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. MSCV-Cre

    Plasmid

    #24064

    Purpose

    Retroviral coexpression of Cre and EGFP

    Depositor

    Dan Littman

    Insert

    Cre
    

    Use

    Cre/Lox and Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    MESV 5' LTR

    Available Since

    Oct. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDD282

    Plasmid

    #66823

    Purpose

    GFP^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

    Insert

    GFP-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans codon-optimized GFP

    Expression

    Worm

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. HDAC4 Flag

    Plasmid

    #13821

    Purpose

    Mammalian expression of human histone deacetylase 4 with flag tag

    Depositor

    Eric Verdin

    Article

    Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-

    Insert

    HDAC4 (HDAC4 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMAL-c2X

    Plasmid

    #75286

    Purpose

    Expresses proteins in the cytoplasm as fusions to maltose-binding protein

    Depositor

    Paul Riggs

    Article

    Walker et al Appl Microbiol Biotechnol. 2010 Sep;88(1

    Type

    Empty backbone

    Tags

    maltose-binding protein

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    July 18, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. SypHer mt

    Plasmid

    #48251

    Purpose

    pH sensor, targeted to mitochondria

    Depositor

    Nicolas Demaurex

    Article

    Poburko et al J Biol Chem. 2011 Apr 1;286(13):11672-84

    Insert

    SypHer
    

    Tags

    2 mitochondrial targeting sequences

    Expression

    Mammalian

    Mutation

    C199S

    Promoter

    CMV

    Available Since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLSSmKate2-N1

    Plasmid

    #31867

    Depositor

    Vladislav Verkhusha

    Article

    Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

    Insert

    LSS-mKate2
    

    Expression

    Mammalian

    Mutation

    K69Y/P131T/S148G/M167D/T183S/M196V

    Promoter

    CMV

    Available Since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. EGFP-Rubicon

    Plasmid

    #28022

    Depositor

    Qing Zhong

    Article

    Sun et al J Biol Chem. 2011 Jan 7. 286(1):185-91.

    Insert

    Rubicon (RUBCN Human)
    

    Tags

    3xFlag and EGFP

    Expression

    Mammalian

    Available Since

    March 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL)

    Plasmid

    #60224

    Purpose

    Expresses Cre recombinase and Renilla luciferase from the EFS promoter and three U6-driven sgRNAs targeting Kras, p53, and Lkb1. Contains KrasG12D HDR donor. AAV backbone.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    sgRNA
    Renilla luciferase
    Cre recombinase
    KrasG12D HDR donor (Kras Mouse)
    

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    Cre-HA and Rluc-P2A

    Expression

    Mammalian

    Promoter

    EFS, No promoter, and U6

    Available Since

    Oct. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA GNSTM-3-RVG-10-Lamp2b-HA

    Plasmid

    #71294

    Purpose

    Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, RVG peptide, 10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.

    Depositor

    Joshua Leonard

    Article

    Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

    Insert

    Lamp2b (LAMP2 Human)
    

    Tags

    GNSTM glycosylation motif, HA, Lamp2 signal pepti…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pmCherry-ATG5

    Plasmid

    #13095

    Depositor

    Roberta Gottlieb

    Article

    Hamacher-Brady et al Cell Death Differ. 2007 Jan;14(1):146-57

    Insert

    ATG5 (Atg5 Mouse)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    Nov. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV:cTNT::Luciferase

    Plasmid

    #69915

    Purpose

    Used to package AAV9:cTNT::luciferase. Specifically transduce cardiomyocytes.

    Depositor

    William Pu

    Article

    Lin et al Circ Res. 2014 Jul 18;115(3):354-63. doi

    Insert

    Firefly luciferase
    

    Use

    AAV

    Promoter

    Chicken cardiac troponin T

    Available Since

    Nov. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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