4,176 results

1. pAG153 FKBP-CFAST11

   Plasmid

   #130813

   Purpose

   Expresses FKBP-CFAST11 in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Tebo et al Nat Commun. 2019 Jun 27;10(1):2822. doi:

   Insert

   FKBP-CFAST11
   

   Tags

   Myc-tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. DR274

   Plasmid

   #42250

   Purpose

   guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence; uses T7 promoter for in vitro transcription

   Depositor

   Keith Joung

   Article

   Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR; Zebrafish expression

   Expression

   Worm

   Promoter

   T7

   Available Since

   Jan. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. Matrix-roGFP

   Plasmid

   #49437

   Purpose

   Expresses thiol redox-sensitive ratiometric sensor roGFP in the mitochondrial matrix of mammalian cells

   Depositor

   Paul Schumacker

   Article

   Waypa et al Circ Res. 2010 Feb 19;106(3):526-35. doi

   Insert

   Matrix-roGFP2
   

   Use

   Adenoviral

   Tags

   Cytochrome c oxidase subunit IV mitochondrial tar…

   Expression

   Mammalian

   Mutation

   C48S, Q80R, S147C, and Q204C in Clontech's G…

   Promoter

   CMV

   Available Since

   Dec. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSpCas9n(BB) (PX460)

   Plasmid

   #48873

   Purpose

   Cas9n (D10A nickase mutant) from S. pyogenes

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Has Service

   Cloning Grade DNA

   Insert

   hSpCas9n
   

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Cas9 D10A nickase mutant

   Promoter

   Cbh

   Available Since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5-FRT/TO-eGFP-53BP1

   Plasmid

   #60813

   Purpose

   mammalian expression vector

   Depositor

   Daniel Durocher

   Article

   Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

   Insert

   53BP1 (TP53BP1 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Available Since

   Dec. 9, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. 7TGP

   Plasmid

   #24305

   Depositor

   Roel Nusse

   Article

   Fuerer et al PLoS One. 2010 . 5(2):e9370.

   Insert

   7xTcf-eGFP // SV40-PuroR
   

   Use

   Lentiviral

   Mutation

   EGFP is GFP with S65T and F64L mutations

   Available Since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEF.myc.ER-E2-Crimson

   Plasmid

   #38770

   Purpose

   In vivo visualization of the endoplasmic reticulum (can be used for colocalization studies)

   Depositor

   Benjamin Glick

   Article

   Strack et al Biochemistry. 2009 Sep 8;48(35):8279-81.

   Insert

   E2-Crimson
   

   Tags

   ER retention signal (KDEL), ER signal sequence (M…

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. PX551

   Plasmid

   #60957

   Purpose

   pAAV-pMecp2-SpCas9-spA (AAV-SpCas9). AAV plasmid expressing Cas9 in neurons under control of truncated mecp2 promoter.

   Depositor

   Feng Zhang

   Article

   Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.103

   Insert

   SpCas9
   

   Use

   AAV and CRISPR

   Tags

   HA

   Expression

   Mammalian

   Promoter

   pMecp2

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR223-ALK

   Plasmid

   #23917

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   ALK (ALK Human)
   

   Use

   Gateway donor vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-Casp3-myc

    Plasmid

    #11813

    Depositor

    Guy Salvesen

    Article

    Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

    Insert

    Caspase 3 (CASP3 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. VP12

    Plasmid

    #72247

    Purpose

    Human expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A and Q926A

    Promoter

    CMV

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNatB (pACYCduet-naa20-naa25)

    Plasmid

    #53613

    Purpose

    Allows expression of the fission yeast NatB complex - chloramphenicol marker

    Depositor

    Dan Mulvihill

    Article

    Johnson et al PLoS One. 2010 Dec 23;5(12):e15801. doi:

    Inserts

    naa20 (naa20 Fission Yeast)
    naa25 (naa25 Fission Yeast)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMSCV-hygro-STING

    Plasmid

    #102598

    Purpose

    Express STING for IFN-beta luciferase reporter assay.

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. mTFP1-N1

    Plasmid

    #54521

    Purpose

    Localization: N1 Cloning Vector, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson, Robert Campbell

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Type

    Empty backbone

    Tags

    mTFP1

    Expression

    Mammalian

    Available Since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-mDlx-ChR2-mCherry-Fishell-3

    Plasmid

    #83898

    Purpose

    ChR2-mCherry fusion expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer element

    Depositor

    Gordon Fishell

    Article

    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

    Has Service

    AAV Retrograde, AAV1, and AAV9

    Insert

    ChR2
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    mDlx

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1 Flag Runx1 FL (P#1790)

    Plasmid

    #14585

    Depositor

    Matija Peterlin

    Article

    Jiang et al Mol Cell Biol. 2005 Dec . 25(24):10675-8

    Insert

    Runx1 (Runx1 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. a1Ha-pcDNA3

    Plasmid

    #45809

    Depositor

    Edward Perez-Reyes

    Article

    Cribbs et al Circ Res. 1998 Jul 13;83(1):103-9.

    Insert

    a1Ha (CACNA1H Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBABE-hygro p53 DD

    Plasmid

    #9058

    Depositor

    Bob Weinberg

    Article

    Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

    Insert

    p53 DD (Trp53 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    DD. Dominant negative mutant carries deletion of…

    Available Since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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19. HA-VHL-pRc/CMV

    Plasmid

    #19999

    Depositor

    William Kaelin

    Article

    Iliopoulos et al Nat Med. 1995 Aug . 1(8):822-6.

    Insert

    von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
    

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wild type

    Available Since

    April 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. mCherry-Rab5a-7

    Plasmid

    #55126

    Purpose

    Localization: Ras GTPase, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Rab5a
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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