We narrowed to 11,959 results for: pcDNA

1. pcDNA3-mCherry-TSC2-NLS

   Plasmid

   #192441

   Purpose

   Encodes mCherry-tagged wild-type TSC2 isoform 5 targeted to the nucleus.

   Depositor

   Jin Zhang

   Article

   Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

   Insert

   mCherry-TSC2-NLS (TSC2 Human)
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-mCherry-TSC2(AA)-NLS

   Plasmid

   #192443

   Purpose

   Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation; targeted to the nucleus.

   Depositor

   Jin Zhang

   Article

   Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

   Insert

   mCherry-TSC2(S939A,T1439A)-NLS (TSC2 Human)
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …

   Promoter

   CMV

   Available since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1-kappa-HA-dL5-BKa

   Plasmid

   #73212

   Purpose

   Expresses HA-dL5(E52D)-BKa (KCNMA1, mSlo alpha subunit) in mammalian cells, with an Igk-leader. (MBIC5, dL5**, FAP)

   Depositor

   Marcel Bruchez

   Article

   Pratt et al Bioconjug Chem. 2015 Sep 16;26(9):1963-7

   Insert

   BKalpha (Kcnma1 Mouse)
   

   Use

   Tags

   dL5**

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-optoB2-LYY-YFP

   Plasmid

   #75271

   Purpose

   G-protein independent version of optoB2 with point mutations at L72F,Y136G,Y224A

   Depositor

   Michael Bruchas

   Article

   Siuda et al Nat Commun. 2015 Sep 28;6:8480. doi: 10.

   Insert

   G-protein independent version of optoB2
   

   Use

   Tags

   eYFP

   Expression

   Mammalian

   Mutation

   point mutations at L72F,Y136G,Y224A

   Promoter

   Available since

   Oct. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA5/FRT_CMV_NES-Caprola_04-mEGFP

   Plasmid

   #194682

   Purpose

   CMV driven expression of the calcium recorder Caprola_04 in mammalian cell lines fused to mEGFP

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_04-mEGFP
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3 c-SRC (250-536)

   Plasmid

   #42208

   Depositor

   Robert Lefkowitz

   Article

   Miller et al J Biol Chem. 2000 Apr 14;275(15):11312-9

   Insert

   c-SRC (250-536) (SRC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   contains amino acids 250-536

   Promoter

   CMV

   Available since

   Feb. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3-MAVS-M1A(KaganF58)

   Plasmid

   #52001

   Purpose

   Expresses the human MAVS CDS containing the point mutation M1A

   Depositor

   Jonathan Kagan

   Article

   Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

   Insert

   MAVS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed Met 1 to Ala

   Promoter

   Available since

   March 24, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3-ELMOD1-myc-His6

   Plasmid

   #201900

   Purpose

   Used for expression of human protein ELMOD1 in mammalian cells

   Depositor

   Richard Kahn

   Insert

   ELMOD1 (ELMOD1 Human)
   

   Use

   Tags

   myc, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(+)/Puro/FA/GFP-LL5BIP

   Plasmid

   #112829

   Purpose

   transient/stable overexpression

   Depositor

   Tomasz Prószyński

   Article

   Niewiadomski et al Matters v. 2, n. 11 (Nov 17, 2016)

   Insert

   LL5BIP (2410002F23Rik Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1-TRBP-L1+D1+L2-myc/His

    Plasmid

    #180599

    Purpose

    Expressing various parts of TRBP

    Depositor

    Kumiko Ui-Tei

    Article

    Takahashi et al Nucleic Acids Res. 2018 Sep 28;46(17):91

    Insert

    TRBP (TARBP2 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Yellow Cameleon-Nano30/pcDNA3

    Plasmid

    #51963

    Purpose

    mammalian expression of Yellow Cameleon-Nano30, an ultrasensitive Ca2+ indicator with Kd = 30 nM

    Depositor

    Takeharu Nagai

    Article

    Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

    Insert

    Yellow Cameleon-Nano30
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3-HtrA2-EGFP S306A

    Plasmid

    #14122

    Depositor

    L. Miguel Martins

    Article

    Martins et al J Biol Chem. 2002 Jan 4. 277(1):439-44.

    Insert

    HtrA2 (HTRA2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S306A

    Promoter

    Available since

    March 7, 2007

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA5 FRT/TO MycLAP-STIL 5A

    Plasmid

    #80267

    Purpose

    mammalian expression plasmid for MycLAP-STIL 5A

    Depositor

    Andrew Holland

    Article

    Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

    Insert

    STIL (STIL Human)
    

    Use

    Tags

    Myc-GFP

    Expression

    Mammalian

    Mutation

    S1103A, S1108A, S1111A, S1116A, T1119A

    Promoter

    CMV

    Available since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5/FRT/TO GFP HSPA14

    Plasmid

    #19488

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    HSPA14 (HSPA14 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1 Myc-huMEKK3 (K391A)

    Plasmid

    #44157

    Depositor

    Xin Lin

    Article

    Blonska et al Mol Cell Biol. 2004 Dec;24(24):10757-65.

    Insert

    MEKK3 (K391A) (MAP3K3 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    K391A, kinase-dead mutant

    Promoter

    Available since

    April 10, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pCDNA3 Flag MKK7a1 (K76A)

    Plasmid

    #14541

    Depositor

    Roger Davis

    Article

    Tournier et al Proc Natl Acad Sci U S A. 1997 Jul 8. 94

    Insert

    MKK7a1(K76A) (Map2k7 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Lys-76Ala

    Promoter

    Available since

    July 25, 2007

    Availability

    Academic Institutions and Nonprofits only

17. GFP Cav2.2 HA D122A pcDNA3

    Plasmid

    #206120

    Purpose

    expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D122A

    Promoter

    CMV

    Available since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Cav2.2 HA E1658A (EIVA) pcDNA3

    Plasmid

    #206114

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658A mutation in the P loop of Domain IV which reduces trafficking to the cell surface

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E1658A

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Cav2.1 EIVA (E1707A) pcDNA3

    Plasmid

    #206125

    Purpose

    expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domain IV to to abolish divalent cation binding

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1a (Cacna1a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E1707A

    Promoter

    CMV

    Available since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Cav2.1 HA EIA (E320A) pcDNA3

    Plasmid

    #206124

    Purpose

    expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domain I to abolish divalent cation binding

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1a (Cacna1a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E320A

    Promoter

    CMV

    Available since

    Oct. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3-HA-REDD1 77-232 (#426)

    Plasmid

    #99921

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    REDD1 (DDIT4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    N-terminal deletion; encodes AA 77-232

    Promoter

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3-sACE2-T92Q-Fc(IgG1)

    Plasmid

    #145170

    Purpose

    Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) glycosylation mutant T92Q

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Fc region of human IgG1

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1-deltaA1-Lock-VWF

    Plasmid

    #124824

    Purpose

    Expresses multimeric human VWF protein without A1 domain and Lock A2 domain. This protein has C-terminal c-Myc tag.

    Depositor

    Sriram Neelamegham

    Article

    Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:

    Insert

    VWF without A1 domain and Lock A2 domain (VWF Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.2/V5 mmu-mir-223

    Plasmid

    #26334

    Depositor

    David Bartel

    Article

    Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

    Insert

    mmu-mir-223 (Mir223 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

25. pCDNA3 Flag MKK7a1 (ala)

    Plasmid

    #14539

    Depositor

    Roger Davis

    Article

    Tournier et al Proc Natl Acad Sci U S A. 1997 Jul 8. 94

    Insert

    MKK7a1(Ala) (Map2k7 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser-198Ala Thr-202Ala

    Promoter

    Available since

    July 25, 2007

    Availability

    Academic Institutions and Nonprofits only

26. pcDNA3 WT MYOC eGLuc2 FLAG

    Plasmid

    #192820

    Purpose

    Expresses wild-type myocilin with a C-terminal enhanced Gaussia luciferase and FLAG tag

    Depositor

    John Hulleman

    Article

    Nakahara et al Curr Eye Res. 2022 Jun;47(6):918-922. do

    Insert

    myocilin (MYOC Human)
    

    Use

    Tags

    FLAG and enhanced Gaussia luciferase (M43I/M110I)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3.1/NDUFS8/myc-His

    Plasmid

    #127489

    Purpose

    Expresses myc-tagged NDUFS8 in mammalian cells

    Depositor

    Shashi Jain, Dieter Wolf

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFS8 (NDUFS8 Human)
    

    Use

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.2/V5 hsa-mir-142

    Plasmid

    #26309

    Depositor

    David Bartel

    Article

    Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

    Insert

    hsa-mir-142 (MIR142 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

29. 3579 pcDNA3 Bad S136A

    Plasmid

    #8798

    Depositor

    Stanley Korsmeyer

    Article

    Harada et al Proc Natl Acad Sci U S A 2001 Aug 14;98(

    Insert

    Bad (Bad Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S136A

    Promoter

    Available since

    July 6, 2005

    Availability

    Academic Institutions and Nonprofits only

30. pCDNA3 -5'cMyc-SMCl- S957A

    Plasmid

    #32368

    Depositor

    Michael Kastan

    Article

    Kim et al Genes Dev. 2002 Mar 1;16(5):560-70.

    Insert

    SMC1A (SMC1A Human)
    

    Use

    Tags

    c-Myc

    Expression

    Mammalian

    Mutation

    S957A

    Promoter

    Available since

    Sept. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

31. pcDNA3-FLAG-DDB1 (1140H)

    Plasmid

    #174849

    Purpose

    Expression of DDB1

    Depositor

    Helen Morrison

    Insert

    DDB1 (DDB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3-S586A-VLCAD-FLAG

    Plasmid

    #55824

    Purpose

    Expresses S586A VLCAD mutant in mammalian cells

    Depositor

    Yoshimi Homma

    Article

    Kabuyama et al Am J Physiol Cell Physiol. 2010 Jan;298(

    Insert

    Very long-chain specific acyl-CoA dehydrogenase (ACADVL Human)
    

    Use

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    changed Serine 586 toAlanine

    Promoter

    CMV

    Available since

    July 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCDNA5/FRT_CMV_NES-Caprola_06-mEGFP

    Plasmid

    #194684

    Purpose

    CMV driven expression of the calcium recorder Caprola_06 in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_06-mEGFP
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3.1(+)-ExRai-AKAR-Kras

    Plasmid

    #118408

    Purpose

    Plasma membrane-targeted excitation-ratiometric biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    ExRai-AKAR-Kras
    

    Use

    Tags

    C-terminal targeting sequence from Kras

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCDNA3 Flag Jip1 JBD

    Plasmid

    #15751

    Purpose

    binds to Jnk

    Depositor

    Roger Davis

    Article

    Dickens et al Science. 1997 Aug 1. 277(5326):693-6.

    Insert

    Jip1 (Jnk binding domain) (Mapk8ip1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    deleted 1-126 and 282-660

    Promoter

    Available since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

36. pcDNA3.1-GFP-2AFP-KDEL

    Plasmid

    #154349

    Purpose

    Mammalian expression of 2AFP

    Depositor

    Wanzhong He

    Article

    Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

    Insert

    2AFP
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3-HCMVgO-8his (TB40/E)

    Plasmid

    #136453

    Purpose

    Mammalian expression plasmid for HCMV glycoprotein O (TB40/E strain) with an 8his tag

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    envelope glycoprotein O (UL74 Human betaherpesvirus 5)
    

    Use

    Tags

    Linker and 8his tag (GGSGHHHHHHHH, includes BamHI…

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3-HCMVgO-8his (Merlin)

    Plasmid

    #136454

    Purpose

    Mammalian expression plasmid for HCMV glycoprotein O (Merlin strain) with an 8his tag

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    glycoprotein O (UL74 Human betaherpesvirus 5)
    

    Use

    Tags

    Linker and 8his tag (GGSGHHHHHHHH, includes BamHI…

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA-zeo-hFJX1-myc

    Plasmid

    #46930

    Purpose

    expression of human FJX1-myc in mammalian cells

    Depositor

    R.D. Beauchamp, Natasha Deane

    Insert

    FJX1 (FJX1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

40. pcDNA3-FRB-LplA(W37V)

    Plasmid

    #35608

    Depositor

    Alice Ting

    Article

    Slavoff et al J Am Chem Soc. 2011 Dec 14;133(49):19769

    Insert

    FRB-LplA(W37V)
    

    Use

    Tags

    FLAG (internal fusion), HA, and His Tag

    Expression

    Mammalian

    Mutation

    LplA has Trp37 to Val mutation

    Promoter

    CMV

    Available since

    March 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge