We narrowed to 2,155 results for: MP

Showing: 331 - 360 of 2155 results

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pDEST14-Cys-SpyCatcher002

Plasmid

#102829

Purpose

Bacterial expression of Cysteine-modified SpyCatcher002 allowing reaction with thiol coupling reagents

Depositor

Mark Howarth

Article

Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

Insert

Cys-SpyCatcher002

Tags

GSSGS, His6, and TEV cleavage site

Expression

Bacterial

Mutation

Cysteine residue in N-terminal region

Promoter

T7

Available Since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pUPD2+p35SoTMV

Plasmid

#170876

Purpose

Phytobrick - p35SoTMV

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

p35SoTMV

Expression

Plant

Mutation

214C>T and 229C>T to remove BbsI restrictio…

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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PU6::unc-119_sgRNA

Plasmid

#46169

Purpose

unc-119 targeting sgRNA

Depositor

John Calarco

Article

Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

Insert

unc-119 targeting sgRNA (unc-119 Synthetic)

Use

CRISPR

Promoter

C. elegans U6 snRNA pol III promoter

Available Since

July 1, 2013

Availability

Academic Institutions and Nonprofits only

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dGFP-Rab3a

Plasmid

#170864

Purpose

Mammalian expression of dGFP-Rab3a.

Depositor

Justin Taraska

Article

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

Rab-3a (Rab3a Mouse)

Tags

dEGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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A547 (miR-146a promoter)

Plasmid

#15091

Depositor

David Baltimore

Article

Taganov et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

Insert

miR-146a promoter

Expression

Mammalian

Available Since

June 6, 2007

Availability

Academic Institutions and Nonprofits only

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pPB-EF1a-MegaGate-DD-Hygro

Plasmid

#175267

Purpose

MegaGate destination with C-terminal DD tag and hygromycin resistance

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Tags

Destabilization Domain

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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p2attNG-H11-short

Plasmid

#51546

Purpose

p2attNG landing pad plasmid with 400 bp homology arms to the human H11 locus (for use with TALEN-assisted recombination).

Depositor

Michele Calos

Article

Zhu et al Nucleic Acids Res. 2013 Dec 4.

Insert

GFP4m

Use

Human targeting

Expression

Mammalian

Available Since

March 4, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX Su(fu) (CT#99)

Plasmid

#13856

Depositor

Cliff Tabin

Article

Pearse et al Dev Biol. 1999 Aug 15. 212(2):323-36.

Insert

Su(fu) (SUFU Chicken)

Tags

GST

Expression

Bacterial

Mutation

Nco I fragment from full length; ~2kbp- ATG to st…

Available Since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only
pRRL-GFPAdGO2-PGK-Neo

Plasmid

#136899

Purpose

Lentivirus for base editing activatable GFP expression in mammalian cells

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

GFPAdGO2

Use

Lentiviral

Tags

2xNLS

Mutation

TGG>TAG

Promoter

SFFV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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dGFP-Rab27a

Plasmid

#170862

Purpose

Mammalian expression of dGFP-Rab27a.

Depositor

Justin Taraska

Article

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

Rab-27a (RAB27A Human)

Tags

dEGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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PB-hEF1a-MG

Plasmid

#175504

Purpose

All-in-one piggyBac transposon destination vector for hEF1a constitutive expression of Mega Gate cloned elements

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

hEF1a

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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14UAS PSD95:GFP 5UAS DSRedExpress

Plasmid

#74315

Purpose

Encodes a PSD95:GFP fusion protein and DsRedExpress from separate UAS cassettes; for long-term two-photon time-lapse imaging of dendrite growth and synaptogenesis in zebrafish

Depositor

Martin Meyer, Stephen Smith

Article

Niell et al Nat Neurosci. 2004 Mar;7(3):254-60. Epub

Inserts

zebrafish PSD95
DsRedExpress

Tags

EGFP

Mutation

Y599H and I793S (please see depositor comment bel…

Available Since

May 19, 2016

Availability

Academic Institutions and Nonprofits only
pUPD+Red-F

Plasmid

#170874

Purpose

Phytobrick - Red-F Luciferase CDS

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

Red-F

Use

Luciferase and Synthetic Biology

Mutation

p.S286Y Red mutant

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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His-GFP-Rabphilin

Plasmid

#170865

Purpose

Mammalian expression of His-GFP-Rabphilin.

Depositor

Justin Taraska

Article

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

Rabphilin-3a (RPH3A Bovine)

Tags

(His)6 and EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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His-GFP-Rab3a

Plasmid

#170863

Purpose

Mammalian expression of His-GFP-Rab3a.

Depositor

Justin Taraska

Article

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

Rab-3a (Rab3a Mouse)

Tags

(His)6 and EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pPB-EF1a-MegaGate-HA-Hygro

Plasmid

#175268

Purpose

MegaGate destination with C-terminal HA tag and hygromycin resistance

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Tags

HA

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pMM136

Plasmid

#127217

Purpose

BeYDV replicon with constitutive Luc expression, MP-delta, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc, MP-delta, sgRNA

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST14-Avitag-SpyCatcher002

Plasmid

#102828

Purpose

Bacterial expression of SpyCatcher002 with a peptide tag enabling site-specific biotinylation

Depositor

Mark Howarth

Article

Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

Insert

Avitag-SpyCatcher002

Tags

Avitag, GSSGS, His6, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pEMPTY::sgRNA2

Plasmid

#165459

Purpose

Escherichia coli – Staphylococcus aureus shuttle vector for plasmid curing in Gram-positive bacteria

Depositor

Inigo Lasa

Article

Dorado-Morales et al mBio. 2021 Feb 23;12(1). pii: mBio.03094

Insert

sgRNA2

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

SP01

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pPMVAu8

Plasmid

#98297

Purpose

Enhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of consitutive promoters.

Depositor

Claudia Vickers

Article

Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

Insert

PRPL4A-EfmvaS-TEFM1-PRPL15A-EfmvaE -TEBS1

Expression

Yeast

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pHKMC1: Empty sgRNA for Cloning

Plasmid

#67720

Purpose

Empty vector for cloning sgRNA. NotI and BamHI for cloning sgRNA. PU6 driven sgRNA.

Depositor

Monica Colaiacovo

Article

Norris et al Genetics. 2015 Oct;201(2):449-58. doi: 1

Type

Empty backbone

Expression

Worm

Promoter

PU6

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only

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pGAL4-CREB S133A

Plasmid

#15222

Depositor

Ugo Moens

Article

Delghandi et al Cell Signal. 2005 Nov . 17(11):1343-51.

Insert

CREB S133A (Creb1 Rat)

Tags

Gal4

Expression

Mammalian

Mutation

Serine 133 mutated to Alanine

Available Since

June 29, 2007

Availability

Academic Institutions and Nonprofits only

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pPB-EF1a-MegaGate-DD-Blast

Plasmid

#175271

Purpose

MegaGate destination with C-terminal DD tag and blasticidin resistance

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Tags

Destabilization Domain

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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dGFP-Rabphilin

Plasmid

#170866

Purpose

Mammalian expression of dGFP-Rabphilin.

Depositor

Justin Taraska

Article

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

Rabphilin-3a (RPH3A Bovine)

Tags

dEGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2+pEstrInd

Plasmid

#170880

Purpose

Phytobrick - Estradiol Inducible Promoter

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

pEstradiol_Inducible

Expression

Plant

Mutation

c.837C>T and c.1353G>A to remove BsaI and E…

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pT2KXIGdeltaIn-MCS-Avi-EGFP-rpl10a

Plasmid

#58380

Purpose

tol2 transgenesis for biotin based TRAP - Avi tagged Ribosome

Depositor

Didier Stainier

Article

Housley et al Development. 2014 Oct;141(20):3988-93. d

Insert

avi-EGFP-rpl10a

Tags

Avi Tag and EGFP

Available Since

Aug. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pRRL-GFPGO2(NGG)-PGK-Neo

Plasmid

#136891

Purpose

Lentivirus for base editing activatable GFP expression in mammalian cells

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

GFPGO2(NGG)

Use

Lentiviral

Tags

2xNLS

Mutation

ATG>ACG

Promoter

SFFV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST14-SpyCatcher002 EQ

Plasmid

#102830

Purpose

Bacterial expression of unreactive version of SpyCatcher002

Depositor

Mark Howarth

Article

Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

Insert

SpyCatcher002 EQ

Tags

GSSGS, His6, and TEV cleavage site

Expression

Bacterial

Mutation

E77Q mutation prevents isopeptide bond formation

Promoter

T7

Available Since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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NFkBm2

Plasmid

#15090

Depositor

David Baltimore

Article

Taganov et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

Insert

miR-146a promoter

Expression

Mammalian

Mutation

2 NFkB binding sites mutated

Available Since

June 6, 2007

Availability

Academic Institutions and Nonprofits only
pUPD2+E-Luc

Plasmid

#170873

Purpose

Phytobrick - E-Luc Luciferase CDS

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

E-Luc

Use

Luciferase and Synthetic Biology

Mutation

c.375C>T to remove BbsI site

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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