We narrowed to 4,504 results for: pcr

1. pCR4-24XMS2SL-stable

   Plasmid

   #31865

   Depositor

   Robert Singer

   Article

   Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

   Insert

   24xMS2 Stem Loops - Stable
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 31, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV-TadA8e-PcrA

   Plasmid

   #221228

   Purpose

   Express HACE Editor as a fusion of TadA-8e adenosine deaminase and PcrA helicase.

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   TadA8e-PcrA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCRISPEY-GAL-Nat

   Plasmid

   #232097

   Purpose

   Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with NatMX selection

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCRISPaint-mNeon-PuroR

   Plasmid

   #174090

   Purpose

   CRISPaint gene tagging

   Depositor

   Veit Hornung

   Article

   Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

   Insert

   mNeonGreen
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCRISPR-PsppA-mCherry

   Plasmid

   #225428

   Purpose

   Plasmid encodes SgRNA, SpCas9 and homologous arms for homologous recombination of mCherry

   Depositor

   Geir Mathiesen

   Article

   Wiull et al Microbiol Spectr. 2025 Feb 4;13(2):e0202

   Insert

   mCherry, Cas9, SgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PsppA upstream of mCherry and Cas9, P3 upstream o…

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCrysβ:ECFP-LexOP:mCherry-KrasG12V

   Plasmid

   #89888

   Purpose

   Human KRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

   Insert

   mCherry-KRasG12V
   

   Use

   Unspecified

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   Aug. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCry2PHR-mCh-LRP6c

   Plasmid

   #42960

   Purpose

   Expresses optogenetically controlled LRP6 (C-terminal domain)

   Depositor

   David Schaffer

   Article

   Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

   Insert

   Cry2PHR-mCherry-LRP6c
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   LPR6 cytoplasmic domain only

   Promoter

   CMV

   Available since

   April 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCR3.1-mGM-CSF

   Plasmid

   #74465

   Purpose

   expression of mGM-CSF in mammalian cells

   Depositor

   Loree Heller

   Article

   Heller et al Melanoma Res. 2000 Dec;10(6):577-83.

   Insert

   granulocyte-monocyte colony-stimulating factor (Csf2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCR3-FLAG-Gephyrin P1

   Plasmid

   #68816

   Purpose

   Flag tagged Geph mammalian expression

   Depositor

   Shiva Tyagarajan

   Article

   Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

   Insert

   Gephyrin (Gphn Rat)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   silent mutations on internal EcoRI site. Gephyrin…

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pCRISPEY-GAL-Kan

    Plasmid

    #232099

    Purpose

    Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with KanMX selection

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCRISPEY-GAL-Hyg

    Plasmid

    #232098

    Purpose

    Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with HygMX selection

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCR2.1 GFR alpha 2

    Plasmid

    #119899

    Purpose

    Mouse GFR alpha 2 In situ hybridization probe

    Depositor

    Wenqin Luo, David Ginty

    Article

    Luo et al Neuron. 2009 Dec 24;64(6):841-56. doi: 1

    Insert

    GDNF family receptor alpha-2 (Gfra2 Mouse)
    

    Use

    In situ hybridization probe

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Pcr4-12xMBS-PBS

    Plasmid

    #52984

    Purpose

    provide MBS-PBS alternating sequence

    Depositor

    Robert Singer

    Article

    Wu et al Sci Rep. 2014 Jan 9;4:3615. doi: 10.1038

    Insert

    12xMBS-PBS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    none

    Available since

    June 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCRISPaint-TagGFP2-PuroR

    Plasmid

    #80970

    Purpose

    CRISPaint gene tagging

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    TagGFP2
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCR3-FLAG-Ubc13-WT

    Plasmid

    #43938

    Depositor

    Bryant Darnay

    Article

    Lamothe et al J Biol Chem. 2007 Feb 9;282(6):4102-12.

    Insert

    ubiquitin-conjugating enzyme E2N (UBE2N Human)
    

    Use

    Tags

    2x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCre-SacB-zeo

    Plasmid

    #107706

    Purpose

    Expresses Cre recombinase for excising chromosomal loxP-Hyg-loxP cassette inserts.

    Depositor

    Kenan Murphy

    Article

    Murphy et al Methods Mol Biol. 2015;1285:177-99. doi:

    Insert

    ble (Zeo resistance)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCRISPaint-2A-TurboGFP-PEST

    Plasmid

    #67180

    Purpose

    CRISPaint universal donor for gene expression quantification using destabilized TurboGFP

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    T2A-TurboGFP-PEST
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCre-P2A-mCherry

    Plasmid

    #212105

    Purpose

    Expression of Cre and mCherry in mammalian cells

    Depositor

    Daekee Lee

    Insert

    Cre recombinase and mCherry
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCR8/GW/TOPO BMI

    Plasmid

    #69796

    Purpose

    Gateway entry vector for BMI

    Depositor

    Gerard Cagney

    Insert

    BMI (BMI1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pCR8/GW/TOPO PCGF2

    Plasmid

    #69791

    Purpose

    Gateway entry vector for PCGF2

    Depositor

    Gerard Cagney

    Insert

    PCGF2 (PCGF2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

21. pCRISPR-Cas9-ScaligD

    Plasmid

    #125688

    Purpose

    Gene knockout/in for actinomycetes

    Depositor

    Tilmann Weber

    Article

    Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

    Insert

    streptomyces codon optimized spCas9, sgRNA casette, and ScaligD
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    ermE*/tipA

    Available since

    Nov. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCRISPR-RFP entry

    Plasmid

    #192278

    Purpose

    Entry vector for cloning MmCas12m spacers. RFP flanked by MmCas12m CRISPR repeats can be digested out with BbsI.

    Depositor

    John van der Oost

    Article

    Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

    Insert

    RFP flanked by type V-M CRISPR repeat sequences
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PJ23119

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCRII-BbsI-sgRNAscaffold

    Plasmid

    #159352

    Purpose

    Expression vector for a customizable guide RNA

    Depositor

    Pim Pijnappel

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCRISPaint-HaloTag-PuroR

    Plasmid

    #80960

    Purpose

    CRISPaint gene tagging

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    HaloTag
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCRISPaint-TagRFP-PuroR

    Plasmid

    #80971

    Purpose

    CRISPaint gene tagging

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    TagRFP
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCR-Flag-IKKalpha

    Plasmid

    #15467

    Depositor

    Hiroyasu Nakano

    Article

    Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

    Insert

    IkB kinase alpha (Chuk Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCRII-1.1-1.10a

    Plasmid

    #69589

    Purpose

    OT-II TCR a chain cDNA in pCRII

    Depositor

    Francis R. Carbone

    Article

    Barnden et al Immunol Cell Biol. 1998 Feb;76(1):34-40.

    Insert

    TCR alpha clone 1.1 (Tcra Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

28. pCRY2FL(deltaNLS)-mCherryN1

    Plasmid

    #26871

    Purpose

    Mammalian expression of cytoplasmic CRY2-mCherry

    Depositor

    Chandra Tucker

    Article

    Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

    Insert

    CRY2 (CRY2 Mustard Weed)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    NLS sequences deleted

    Promoter

    CMV

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCry2PHR-mCh-RhoA

    Plasmid

    #42958

    Purpose

    Expresses optogenetically controlled RhoA

    Depositor

    David Schaffer

    Article

    Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

    Insert

    Cry2PHR-mCherry-RhoA (RHOA Mustard Weed, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCRISPaint-3xP3-GFP

    Plasmid

    #130277

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes 3xP3-GFP visible eye marker and multiple cloning site.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    3xP3-GFP
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge