We narrowed to 3,314 results for: GCT

1. pSUC01

   Plasmid

   #177204

   Purpose

   chromogenic reporter for gene knockout in Streptomyces

   Depositor

   Wenjun Guan

   Article

   Dong et al J Microbiol Biotechnol. 2021 Dec 28;31(1

   Insert

   SaindC
   

   Use

   Synthetic Biology

   Tags

   NONE

   Expression

   Bacterial

   Mutation

   NONE

   Promoter

   TGTTCACATTCGAACGGTCTCTGCTTTGACAACATGCTGTGCGGTGTTG…

   Available since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTargetF

   Plasmid

   #62226

   Purpose

   Constitutive expression of sgRNA without donor editing template DNA

   Depositor

   Sheng Yang

   Article

   Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii

   Insert

   sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pij23119

   Available since

   March 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPEgRNA

   Plasmid

   #172716

   Purpose

   Delivery of guide RNA for prime editing

   Depositor

   Tilmann Weber

   Article

   Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

   Insert

   gRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Rictor_2 shRNA

   Plasmid

   #1854

   Depositor

   David Sabatini

   Article

   Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

   Insert

   Rictor (RICTOR Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 7, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pnsgRNA

   Plasmid

   #172717

   Purpose

   Delivery of guide RNA for prime editing

   Depositor

   Tilmann Weber

   Article

   Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

   Insert

   J23119-gRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CMV-FLEX-SaCas9-U6-sgDrd1

   Plasmid

   #159917

   Purpose

   Mutagenesis of Drd1

   Depositor

   Larry Zweifel

   Insert

   Drd1 (Drd1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. PLKO.1-USP10-3'UTR

   Plasmid

   #136056

   Purpose

   USP10 shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (GCCTCTCTTTAGTGGCTCTTT)

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   USP10 (USP10 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. tet-pLKO.puro_shHuR CDS

   Plasmid

   #110411

   Purpose

   TRCN0000276129 (Target CGAGCTCAGAGGTGATCAAAG), silence human ELAVL1 (HuR) gene, doxycycline inducible, puromycin selection

   Depositor

   Sandra Martha Gomes Dias

   Insert

   ELAVL1 (HuR)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1/TO (RNA PolIII)

   Available since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. PLKO.1-STAU1-3'UTR

   Plasmid

   #136049

   Purpose

   STAU1 shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (CCATCACCACTGCTTTCTCTT)

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   STAU1 (STAU1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAH233

    Plasmid

    #121144

    Purpose

    Expression of gRNA with BbsI placeholder for target cloning

    Depositor

    Katsunori Tanaka

    Article

    Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.

    Insert

    gRNA cassette
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    S.pombe rrk1

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. TLCV2 - sgAP2M1

    Plasmid

    #202758

    Purpose

    Encodes Doxycycline inducible Cas9 and sgRNA targeting AP2M1

    Depositor

    Christopher Counter

    Article

    Zimmerman et al Nat Commun. 2025 Mar 6;16(1):2254. doi:

    Insert

    gRNA targeting AP2M1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. LentiCRISPR v2 sgZER1-2

    Plasmid

    #191550

    Purpose

    Expression of spCas9 and sgRNA targetting ZER1

    Depositor

    Elizabeth White

    Article

    Nouel et al mBio. 2022 Nov 8:e0203322. doi: 10.1128/

    Insert

    spCas9 (ZER1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEDJ8

    Plasmid

    #177271

    Purpose

    Double gRNA for RNH1 RNH201 knock-out

    Depositor

    Michael Jensen

    Article

    Jensen et al Nucleic Acids Res. 2021 Sep 7;49(15):e88

    Insert

    gRNA_RNH1_RNH201
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    SNR52

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAW214

    Plasmid

    #113236

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader gCtag mutant

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GGTAG->gCtag

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJB046

    Plasmid

    #22627

    Depositor

    Lynn Hudson

    Article

    Berndt et al J Biol Chem. 1992 Jul 25. 267(21):14730-

    Insert

    human genomic PLP promoter region (PLP1 Human)
    

    Use

    Cat reporter plasmid

    Tags

    Expression

    Mutation

    Site 1 (NF-like) GGCTGTCAATC to cGCTaTgAATg

    Promoter

    Available since

    Nov. 24, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pSWAP_Entry_T1

    Plasmid

    #184863

    Purpose

    pSwap plasmid part containing the T1 sgRNA module for the Swap and Drop recombination system.

    Depositor

    Patrick Sobetzko

    Article

    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

    Insert

    lacZalpha,ccdB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pAAV-CMV-FLEX-SaCas9-U6-sgPenk

    Plasmid

    #159916

    Purpose

    Mutagenesis of Penk

    Depositor

    Larry Zweifel

    Insert

    Penk (Penk Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLH-stsgRNA3.1

    Plasmid

    #64118

    Purpose

    Vector for expression of St1 sgRNA3.1 in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    St1 sgRNA3.1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. OA-1067B

    Plasmid

    #200252

    Purpose

    pBac-U6-gRNA(doublesex)-3xp3-tdTomato

    Depositor

    Omar Akbari

    Article

    Li et al bioRxiv. 2023 Nov 15:2023.04.18.537404.

    Insert

    4 gRNAs targeting Doublesex
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTarget12f1

    Plasmid

    #171183

    Purpose

    Constitutive expression of sgRNA without donor DNA

    Depositor

    Kohsuke Honda

    Article

    Okano et al J Biosci Bioeng. 2021 Aug;132(2):120-124

    Insert

    sgRNA scaffold in the CRISPR/Cas12f1 system
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119(SpeI) promoter

    Available since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pLL3.7 Scr shRNA

    Plasmid

    #59299

    Purpose

    non-targeting shRNA

    Depositor

    Ulrike Heberlein

    Article

    Lasek et al Genes Brain Behav. 2007 Nov;6(8):728-35.

    Insert

    Scr shRNA
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    Oct. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

22. Lenti-sgNeo2/Cre

    Plasmid

    #89652

    Purpose

    Expresses a Neomycin-targeting gRNA and Cre-recombinase

    Depositor

    Monte Winslow

    Article

    Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

    Insert

    gRNA targeting Neomycin
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLKO-Tet-On-HDAC1-shRNA2

    Plasmid

    #90241

    Purpose

    Lentivirus for expression of shRNA2 against human HDAC1 (Dox-inducible)

    Depositor

    Roland Friedel

    Insert

    HDAC1 (HDAC1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. OA-1050G (white)

    Plasmid

    #132420

    Purpose

    express arrays of gRNA targeting White under dU6-3 promoter

    Depositor

    Omar Akbari

    Article

    Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

    Insert

    white gRNA array
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6-3

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pUC19-U6-AasgRNA

    Plasmid

    #121954

    Purpose

    Mammalian expression, Genome editing, gRNA scaffold

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    AaCas12b single chimeric gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCBC-DT1T2.2

    Plasmid

    #134752

    Purpose

    PCR template for sgRNA cloning

    Depositor

    Qi-Jun Chen

    Article

    Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

    Insert

    sgRNA-AtU6_29p
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCBC-MT1T2.2

    Plasmid

    #134751

    Purpose

    PCR template for sgRNA cloning

    Depositor

    Qi-Jun Chen

    Article

    Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

    Insert

    sgRNA-TaU3p
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLH-stsgRNA2.1

    Plasmid

    #64117

    Purpose

    Vector for expression of St1 sgRNA2.1 in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    St1 sgRNA2.1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pUPD-Cyc1t (17-21)

    Plasmid

    #101700

    Purpose

    Cyc1t in domestication vector

    Depositor

    Elena Caro Bernat

    Article

    Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

    Insert

    GCTT-[terminator of S. cerevisiae YJR048W (190 bp)]-CGCT
    

    Use

    Yeast expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Neg. Ctrl_sgRNA

    Plasmid

    #111298

    Purpose

    CRISPR KO murine neg. ctrl.

    Depositor

    Vamsi Mootha

    Article

    Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

    Insert

    NegCtrl sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLH-stsgRNA1.1

    Plasmid

    #64116

    Purpose

    Vector for expression of St1 sgRNA1.1 in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    St1 sgRNA1.1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pSDMA66

    Plasmid

    #128355

    Purpose

    gRNA ribozyme construct with Cryptococcus neoformans ACT1 promter and TRP1 terminator. Following ribozyme cleavage, a gRNA targeting ADE2 is liberated.

    Depositor

    James Fraser

    Article

    Arras et al PLoS One. 2016 Oct 6;11(10):e0164322. do

    Insert

    Noureothricin (NAT)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    ACT1

    Available since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLKO.1P shMOCS3_1

    Plasmid

    #102973

    Purpose

    Suppression of MOCS3

    Depositor

    Richard Possemato

    Article

    Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

    Insert

    shMOCS3_1 (MOCS3 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pSDMA67

    Plasmid

    #128356

    Purpose

    gRNA ribozyme construct with Cryptococcus neoformans ACT1 promter and TRP1 terminator. Following ribozyme cleavage, a gRNA targeting ADE2 is liberated.

    Depositor

    James Fraser

    Article

    Arras et al PLoS One. 2016 Oct 6;11(10):e0164322. do

    Insert

    Noureothricin (NAT)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    ACT1

    Available since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pUPD-ADH2t (17-21)

    Plasmid

    #101701

    Purpose

    ADH2t in domestication vector

    Depositor

    Elena Caro Bernat

    Article

    Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

    Insert

    GCTT-[terminator of S. cerevisiae YMR303C (376 bp)]-CGCT
    

    Use

    Yeast expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLentiCRISPRv1.linker_KO

    Plasmid

    #164688

    Purpose

    For CRISPR knockout of miR-144~451 linker region by lentiviral delivery of Cas9 and linker gRNA

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Insert

    miR-144~451 linker CRISPR KO gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pSUPERIOR.puro-shREV3-4

    Plasmid

    #38029

    Depositor

    Rolf Stahel, Emanuela Felley-Bosco

    Article

    Knobel et al Neoplasia. 2011 Oct;13(10):961-70.

    Insert

    shREV3-4 (REV3L Mouse, human, Human, Rat)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

38. pUC19-U6-AmsgRNA

    Plasmid

    #121956

    Purpose

    Mammalian expression, Genome editing, gRNA scaffold

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    AmCas12b single chimeric gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pG3GB411

    Plasmid

    #134748

    Purpose

    pGreen3 CRISPR/Cas9

    Depositor

    Qi-Jun Chen

    Article

    Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

    Insert

    CRISPR/Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    gRNA- OsU3, zCas9-Ubi1

    Available since

    Dec. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. nSR100L4

    Plasmid

    #35174

    Depositor

    Benjamin Blencowe

    Article

    Calarco et al Cell. 2009 Sep 4;138(5):898-910.

    Insert

    MmnSR100
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only