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Showing: 551 - 600 of 903 results

  12. pCSN075

    Plasmid
    #166732
    Purpose
    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS and Mxi, codon optimized for Saccharomyces cerevisiae
    Depositor
    Rene Verwaal
    Article
    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312
    Insert
    dCas12a-NLS-Mxi1
    Use
    CRISPR
    Tags
    Mxi1 and SV40 NLS
    Expression
    Yeast
    Mutation
    E925A
    Promoter
    S. cerevisiae TEF1
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    166732-plasmid-map-sequence-id-332206

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  14. p414-TEF1p-Cas9-CYC1t

    Plasmid
    #43802
    Purpose
    A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the TEF1 promoter
    Depositor
    George Church
    Article
    Dicarlo et al Nucleic Acids Res
    Insert
    Human Optimized S. pyogenes Cas9
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1 promoter
    Available since
    March 15, 2013
    Availability
    Academic Institutions and Nonprofits only
    43802-plasmid-map-sequence-id-333570

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  20. pY2

    Plasmid
    #218115
    Purpose
    Activity reporter backbone with an empty protease cassette (BsaI) under (lexA-box)3PminCYC1 promoter and an empty substrate cassette (BsmBI) under pGAL1 promoter.
    Depositor
    Carl Denard
    Article
    Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    218115-plasmid-map-sequence-id-431070

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  21. pY3

    Plasmid
    #218116
    Purpose
    Activity reporter backbone with an empty protease cassette (BsaI) and an empty substrate cassette (BsmBI), both under a divergent pGAL1-10 promoter.
    Depositor
    Carl Denard
    Article
    Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    218116-plasmid-map-sequence-id-431074

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  29. pRMH120

    Plasmid
    #40593
    Purpose
    Expresses Gal4BD-CRY2PHR and Gal4AD-CIB1 for light-inducible protein expression
    Depositor
    Chandra Tucker
    Article
    Hughes et al Methods. 2012 Aug 15.
    Inserts
    CRY2 (CRY2 Mustard Weed)
    CIB1 (CIB1 Mustard Weed)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ADH1 and TEF
    Available since
    Oct. 29, 2012
    Availability
    Academic Institutions and Nonprofits only
    40593-plasmid-map-sequence-id-52556

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  30. pSDMA66

    Plasmid
    #128355
    Purpose
    gRNA ribozyme construct with Cryptococcus neoformans ACT1 promter and TRP1 terminator. Following ribozyme cleavage, a gRNA targeting ADE2 is liberated.
    Depositor
    James Fraser
    Article
    Arras et al PLoS One. 2016 Oct 6;11(10):e0164322. do
    Insert
    Noureothricin (NAT)
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    ACT1
    Available since
    Aug. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    128355-plasmid-map-sequence-id-254590

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  33. pCSN074

    Plasmid
    #166731
    Purpose
    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, codon optimized for Saccharomyces cerevisiae
    Depositor
    Rene Verwaal
    Article
    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312
    Insert
    dCas12a-NLS
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Yeast
    Mutation
    E925A
    Promoter
    S. cerevisiae TEF1
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    166731-plasmid-map-sequence-id-332203

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  34. pSDMA67

    Plasmid
    #128356
    Purpose
    gRNA ribozyme construct with Cryptococcus neoformans ACT1 promter and TRP1 terminator. Following ribozyme cleavage, a gRNA targeting ADE2 is liberated.
    Depositor
    James Fraser
    Article
    Arras et al PLoS One. 2016 Oct 6;11(10):e0164322. do
    Insert
    Noureothricin (NAT)
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    ACT1
    Available since
    Aug. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    128356-plasmid-map-sequence-id-254064

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  39. pCSN069

    Plasmid
    #166728
    Purpose
    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to Mxi1, codon optimized for Saccharomyces cerevisiae
    Depositor
    Rene Verwaal
    Article
    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312
    Insert
    dCas12a-Mxi1
    Use
    CRISPR
    Tags
    Mxi1
    Expression
    Yeast
    Mutation
    E925A
    Promoter
    S. cerevisiae TEF1
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    166728-plasmid-map-sequence-id-332209

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Showing: 551 - 600 of 903 results