We narrowed to 9,302 results for: try
-
Plasmid#128650PurposeEntry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymesDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only
-
CALCOCO1 L140A-mycDDK
Plasmid#137767PurposeCALCOCO1 CLIR mutantDepositorInsertCALCOCO1 L140A
UseTagsmycDDKExpressionMammalianMutationL140APromoterCMVAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
CALCOCO1 R12H-mycDDK
Plasmid#137770PurposeCALCOCO1 R12H mutantDepositorInsertCALCOCO1 R12H
UseTagsmycDDKExpressionMammalianMutationR12HPromoterCMVAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 LC3B2 no-stop
Plasmid#123207PurposeGateway entry clone encoding human MAP1LC3B2 lacking stop codon, suitable for C-terminal taggingDepositorInsertMicrotubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)
UseGateway entry vector / entry cloneTagsExpressionMutationStop codon removedPromoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRPa-c6MYCn
Plasmid#69242PurposeTet-inducible RRNA promoter driven plasmid for expression of N or C-terminally c-Myc tagged protein from a specific RRNA spacer locus in T. brucei.DepositorTypeEmpty backboneUseExpression in trypanosoma bruceiTagscMycExpressionMutationPromoterRRNA promoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRP-c6MYCn
Plasmid#69243PurposeTet-inducible RRNA promoter driven plasmid for inducible expression of N- or C-terminally c-Myc tagged protein from a random RRNA spacer locus in T. brucei.DepositorTypeEmpty backboneUseExpression in trypanosoma bruceiTagscMycExpressionMutationPromoterRRNA promoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRPa-iSL(MCS3+4)
Plasmid#69246PurposeTet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a specific RRNA spacer locus in T. brucei.DepositorTypeEmpty backboneUseExpression in trypanosoma bruceiTagsExpressionMutationPromoterRRNA promoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(7AW)RS
Plasmid#207516PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 7-Aza-L-Tryptophan (7AW) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(7FTrp)RS
Plasmid#177310PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-tdTomato-WPRE
Plasmid#133786PurposeCre-dependent tdTomato fluorescent reporter for cell labelingDepositorInsertpAAV-EF1a-DIO-tdTomato-WPRE
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceJune 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 LC3C WT
Plasmid#123078PurposeGateway entry clone encoding human MAP1LC3C wild-typeDepositorInsertMicrotubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)
UseGateway entry vector / entry cloneTagsExpressionMutationPromoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-LwaCas13acrRNA-BsmBIcassette (LTH151)
Plasmid#171129PurposeLwaCas13a crRNA entry vector for pU6 expression (need to clone in spacer into BsmBI sites)DepositorInsertU6 promoter entry plasmid for LwaCas13a crRNA cloning
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTrex-Neo-tdTomato
Plasmid#47975PurposeTrasgenically expresses tandem tdtomato fluorescent protein in T. cruziDepositorInserttdTomato
UseTrypanasoma cruzi expressionTagsExpressionMutationPromoterT. cruzi rRNAAvailable sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1R1
Plasmid#97323PurposeNanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional R residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.DepositorInsertMSP1D1R1
UseTags6xHis - TEVExpressionBacterialMutationAddition of R residue after T25PromoterT7Available sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1G1
Plasmid#97325PurposeNanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional G residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.DepositorInsertMSP1D1G1
UseTags6xHis - TEVExpressionBacterialMutationAddition of G residue after T25PromoterT7Available sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 GABARAP WT
Plasmid#123080PurposeGateway entry clone encoding human GABARAP wild-typeDepositorInsertGamma-aminobutyric acid receptor-associated protein (GABARAP Human)
UseGateway entry vector / entry cloneTagsExpressionMutationPromoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSMOX-mNG::LacI-V3
Plasmid#210224PurposeIntegrates a T7 RNA polymerase, tetracycline repressor and an mNeongreen Lac repressor fusion protein with a puromycin drug selection marker into the tubulin locus.DepositorInsertmNeongreen Lac repressor fusion protein
UseTrypanosoma brucei expressionTagsExpressionMutationPromoterPol II readthrough transcription after integrationAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
MC177VSG221RNAi
Plasmid#59730PurposeFor use in trypanosome cell lines already expressing T7 polymerase and Tet-repressor. Tetracycline induction results in the production of dsRNA targeting VSG221.DepositorInsertVSG221 RNAi fragment
UseRNAiTagsExpressionMutationPromoteropposing T7 promotersAvailable sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1T1
Plasmid#97327PurposeNanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional T residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.DepositorInsertMSP1D1T1
UseTags6xHis - TEVExpressionBacterialMutationAddition of T residue after T25PromoterT7Available sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
592 p3e-EST etv2 3’UTR
Plasmid#49003PurposeZebrafish EST annotated length etv2 3' UTR in gateway 3' Entry vectorDepositorInsertets variant gene 2 (etsrp Zebrafish)
UseGateway 3' entryTagsExpressionMutationonly the 3' UTRPromoter3' gateway entry vectorAvailable sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-hyg-OsAID (p5110)
Plasmid#239360PurposepPOTv7 based plasmid template for addition of a Rice Auxin Inducible Degradation (AID) domain to genes in trypanosomes with selection using hygromycinDepositorInsertsRice Auxin Inducible Degradation domain
Auxin Inducible Degradation domain
UseBacterialTagsExpressionMutationPromoternoneAvailable sinceJuly 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPOTv6-puro-3Ty:OsAID:3Ty (p5229)
Plasmid#239361PurposepPOTv6 based plasmid template for addition of Ty-epitope tagged Rice Auxin Inducible Degradation (AID) domain to genes in trypanosomes with selection using puromycinDepositorInsertRice Auxin inducible degradation domain
UseBacterialTags3 x Ty epitope tagExpressionMutationPromoternoneAvailable sinceJuly 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-hyg-AtAID:HA (p5339)
Plasmid#239363PurposepPOTv7 based plasmid template for addition of HA-epitope tagged Rice Auxin Inducible Degradation (AID) domain to genes in trypanosomes with selection using hygromycinDepositorInsertArabidopsis Auxin Inducible Degradation domain
UseBacterialTags1 x HA epitopeExpressionMutationPromoternoneAvailable sinceJuly 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPOTv6-hyg-3Ty:OsAID:3Ty (p5230)
Plasmid#239362PurposepPOTv6 based plasmid template for addition of Ty-epitope tagged Rice Auxin Inducible Degradation (AID) domain to genes in trypanosomes with selection using puromycinDepositorInsertRice Auxin inducible degradation domain
UseBacterialTags3 x Ty epitope tagExpressionMutationPromotern/aAvailable sinceJuly 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
-
pDONR223 LC3B WT
Plasmid#123072PurposeGateway entry clone encoding human MAP1LC3B wild-typeDepositorInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
UseGateway entry vector / entry cloneTagsExpressionMutationPromoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 LC3A WT
Plasmid#123069PurposeGateway entry clone encoding human MAP1LC3A wild-typeDepositorInsertMicrotubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)
UseGateway entry vector / entry cloneTagsExpressionMutationPromoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(4CNW)RS
Plasmid#222871PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 4-Cyano-L-Tryptophan (4-CN-Trp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
UseTagsExpressionBacterialMutationPromoterT7Available sinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(6CNW)RS
Plasmid#222873PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 6-Cyano-L-Tryptophan (6-CN-Trp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
UseTagsExpressionBacterialMutationPromoterT7Available sinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 HCoV-OC43 NSP1_nostop
Plasmid#168687PurposeGateway-compatible Entry vectorDepositorInsertNSP1 (ORF1ab )
UseOtherTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 MERS-CoV NSP15_stop
Plasmid#168640PurposeGateway-compatible Entry vectorDepositorInsertNSP15 (orf1ab )
UseOtherTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 GABARAPL1 WT
Plasmid#123083PurposeGateway entry clone encoding human GABARAPL1 wild-typeDepositorInsertGamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
UseGateway entry vector / entry cloneTagsExpressionMutationPromoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 GABARAPL2 WT
Plasmid#123086PurposeGateway entry clone encoding human GABARAPL2 wild-typeDepositorInsertGamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)
UseGateway entry vector / entry cloneTagsExpressionMutationPromoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 HCoV-OC43 NSP5_nostop
Plasmid#168690PurposeGateway-compatible Entry vectorDepositorInsertNSP5 (ORF1ab )
UseOtherTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 HCoV-OC43 NSP12_nostop
Plasmid#168696PurposeGateway-compatible Entry vectorDepositorInsertNSP12 (ORF1ab )
UseOtherTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 HCoV-HKU1 NSP1_nostop
Plasmid#168570PurposeGateway-compatible Entry vectorDepositorInsertNSP1 (ORF1ab )
UseOtherTagsExpressionMutationPromoterAvailable sinceJan. 20, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 LC3A G120A
Plasmid#123070PurposeGateway entry clone encoding human MAP1LC3A G120ADepositorInsertMicrotubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)
UseGateway entry vector / entry cloneTagsExpressionMutationGlycine 120 to AlaninePromoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 GABARAP G116A
Plasmid#123081PurposeGateway entry clone encoding human GABARAP G116ADepositorInsertGamma-aminobutyric acid receptor-associated protein (GABARAP Human)
UseGateway entry vector / entry cloneTagsExpressionMutationGlycine 116 to AlaninePromoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 LC3B G120A
Plasmid#123073PurposeGateway entry clone encoding human MAP1LC3B G120ADepositorInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
UseGateway entry vector / entry cloneTagsExpressionMutationGlycine 120 to AlaninePromoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 HCoV-NL63 E_stop
Plasmid#168684PurposeGateway-compatible Entry vectorDepositorInsertE (HCNV63gp4 )
UseOtherTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 HCoV-NL63 E_nostop
Plasmid#168666PurposeGateway-compatible Entry vectorDepositorInsertE (HCNV63gp4 )
UseOtherTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 LC3C G126
Plasmid#123079PurposeGateway entry clone encoding human MAP1LC3C G126DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)
UseGateway entry vector / entry cloneTagsExpressionMutationDeleted amino acids 127-147. Stop codon after G12…PromoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 GABARAP no-stop
Plasmid#123209PurposeGateway entry clone encoding human GABARAP lacking stop codon, suitable for C-terminal taggingDepositorInsertGamma-aminobutyric acid receptor-associated protein (GABARAP Human)
UseGateway entry vector / entry cloneTagsExpressionMutationStop codon removedPromoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 LC3C no-stop
Plasmid#123208PurposeGateway entry clone encoding human MAP1LC3C lacking stop codon, suitable for C-terminal taggingDepositorInsertMicrotubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)
UseGateway entry vector / entry cloneTagsExpressionMutationStop codon removedPromoterCMVAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 GABARAPL1 G116A
Plasmid#123084PurposeGateway entry clone encoding human GABARAPL1 G116ADepositorInsertGamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
UseGateway entry vector / entry cloneTagsExpressionMutationGlycine 116 to AlaninePromoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET30Xa-SBL1(160S)
Plasmid#66872PurposeExpresses WTsoybean lipoxygenase-1 160S with N-term HistagDepositorInsertsoybean lipoxygenase-1 wild type (NEWENTRY Glycine max)
UseTagsHistag from pET30Xa-LICExpressionBacterialMutationnonePromoterT7lacAvailable sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 GABARAP G116
Plasmid#123082PurposeGateway entry clone encoding human GABARAP G116DepositorInsertGamma-aminobutyric acid receptor-associated protein (GABARAP Human)
UseGateway entry vector / entry cloneTagsExpressionMutationDeleted amino acid 117. Stop codon after G116PromoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1T2
Plasmid#97328PurposeNanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include two additional T residues shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.DepositorInsertMSP1D1T2
UseTags6xHis - TEVExpressionBacterialMutationAddition of two T residues after T25PromoterT7Available sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(7CNW)RS
Plasmid#222874PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 7-Cyano-L-Tryptophan (7-CN-Trp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(5CNW)RS
Plasmid#222872PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 5-Cyano-L-Tryptophan (5-CN-Trp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
UseTagsExpressionBacterialMutationPromoterT7Available sinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only