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pET21d-colEdes11/Imdes11

Plasmid

#107119

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des11

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes11/Imdes11

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21d-colEdes12/Imdes12

Plasmid

#107120

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des12

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes12/Imdes12

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21d-colEdes13/Imdes13

Plasmid

#107121

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des13

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes13/Imdes13

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21d-colEdes14/Imdes14

Plasmid

#107122

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des14

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes14/Imdes14

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21d-colEdes15/Imdes15

Plasmid

#107123

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des15

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes15/Imdes15

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21d-colEdes16/Imdes16

Plasmid

#107124

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des16

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes16/Imdes16

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21d-colEdes17/Imdes17

Plasmid

#107125

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des17

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes17/Imdes17

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21d-colEdes18/Imdes18

Plasmid

#107126

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des18

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes18/Imdes18

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21d-colEdes1/Imdes1

Plasmid

#107108

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des1

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes1/Imdes1

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21d-colEdes2/Imdes2

Plasmid

#107109

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des2

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes2/Imdes2

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21d-colEdes3/Imdes3

Plasmid

#107110

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des3

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes3/Imdes3

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21d-colEdes3.5/Imdes3.5

Plasmid

#107111

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des3.5

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes3.5/Imdes3.5

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21d-colEdes4/Imdes4

Plasmid

#107112

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des4

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes4/Imdes4

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21d-colEdes5/Imdes5

Plasmid

#107113

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des5

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes5/Imdes5

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21d-colEdes6/Imdes6

Plasmid

#107114

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des6

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes6/Imdes6

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
IDP378

Plasmid

#87005

Purpose

plasmid for cell culture expression of SYNseq components

Depositor

Anthony Zador

Article

Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

Insert

preLib-bfp-4xboxB

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pUT1191-HQ858811

Plasmid

#27893

Depositor

John Gray, Erich Grotewold

Article

Yilmaz et al Plant Physiol. 2009 Jan;149(1):171-80. d

Insert

MYB-related transcription factor encoded by Os05g0589400 LOC_Os05g51160

Use

Entry vector

Available Since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pUT1508-HQ858840

Plasmid

#27922

Depositor

John Gray, Erich Grotewold

Article

Yilmaz et al Plant Physiol. 2009 Jan;149(1):171-80. d

Insert

MYB-related transcription factor encoded by Os08g0510700 LOC_Os08g39980

Use

Entry vector

Available Since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pUT1510-HQ858841

Plasmid

#27923

Depositor

John Gray, Erich Grotewold

Article

Yilmaz et al Plant Physiol. 2009 Jan;149(1):171-80. d

Insert

C3H-related transcription factor encoded by Os04g0671800 LOC_Os04g57600

Use

Entry vector

Available Since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
Lifeact-mScarlet3-S2

Plasmid

#246873

Purpose

Expression of mScarelt3-S2 fused with Lifeact. This construct is used for imaging.

Depositor

Pingyong Xu

Article

Ding et al Nat Methods. 2025 Nov 26. doi: 10.1038/s

Insert

mScarlet3-S2

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-ATR WT

Plasmid

#31611

Depositor

Aziz Sancar

Article

Jiang et al Mol Cell Biol. 2006 Jan . 26(1):39-49.

Insert

ATR (ATR Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQAT48

Plasmid

#223420

Purpose

T-DNA vector for Mb2Cas12a-RVRR based mutagenesis for dicot plants; more relaxed PAM requirements; Mb2Cas12a-RVRR and the crRNA were driven by separate 2x35s promoter; Kanamycin for plants selection.

Depositor

Yiping Qi

Article

Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

Insert

2x35s-Mb2Cas12a-RVRR-2x35s-crRNA scaffold

Use

CRISPR

Tags

NLS

Expression

Plant

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
HER2Nb-C AND (A OR T)-SpyTag

Plasmid

#243790

Purpose

Expresses multifunctional construct enabling the AND(OR)-gated release of a HER2 Nanobody from materials in response to HRV-3C AND (sortase eSrtA(2A9) OR TEV) treatment.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

HER2 nanobody

Tags

6x His tag and SsrA

Expression

Bacterial

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
mScarlet3-S2-Sec61β

Plasmid

#246874

Purpose

Expression of mScarelt3-S2 fused with Sec61β. This construct is used for imaging.

Depositor

Pingyong Xu

Article

Ding et al Nat Methods. 2025 Nov 26. doi: 10.1038/s

Insert

mScarlet3-S2

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
CuGFP-ATG8(416)

Plasmid

#49423

Purpose

Expresses ATG8 fused at the N terminus to GFP under the control of the CUP1 promoter.

Depositor

Daniel Klionsky

Article

Wang et al J Biol Chem. 2012 Nov 2;287(45):37964-72

Insert

autophagy-related 8 (ATG8 Budding Yeast)

Tags

GFP

Expression

Bacterial and Yeast

Promoter

CUP1

Available Since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
Flag-NRF1

Plasmid

#34707

Depositor

Raymond Deshaies

Article

Radhakrishnan et al Mol Cell. 2010 Apr 9;38(1):17-28.

Insert

NRF1 (NFE2L1 Human)

Use

Retroviral

Tags

3XFlag

Expression

Mammalian

Promoter

LTR

Available Since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only
Orai1 YFP

Plasmid

#19756

Depositor

Anjana Rao

Article

Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

Insert

Orai1 (ORAI1 Human)

Tags

YFP

Expression

Mammalian

Available Since

Oct. 28, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
Orai1 CFP

Plasmid

#19757

Depositor

Anjana Rao

Article

Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

Insert

Orai1 (ORAI1 Human)

Tags

CFP

Expression

Mammalian

Available Since

Nov. 12, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-CAG-hChR2-H134R-tdTomato

Plasmid

#28017

Purpose

AAV-mediated expression of hChR2(H134R) for neuronal excitation.

Depositor

Karel Svoboda

Article

Mao et al Neuron. 2011 Oct 6;72(1):111-23.

Has Service

AAV Retrograde

Insert

humanized ChR2-H134R-tdTomato

Use

AAV; Adeno associate viral

Tags

tdTomato

Mutation

H134R

Promoter

CAG

Available Since

Nov. 30, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
miReporter-CAG

Plasmid

#82478

Purpose

microRNA reporter relying on a bidirectional CAG-based promoter. Contains MCS downstream of H2B-mCherry and H2B-Citrine to insert miRNA binding sites. Contains a Hygromycin selection cassette.

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2016 May 6;11(5):e0155177. doi

Insert

H2B-mCherry and H2B-Citrine. HygroR

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBPp65ADZpUw

Plasmid

#26234

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

p65AD-Zip (RELA Human)

Expression

Insect

Available Since

Oct. 7, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pBPLexA::p65Uw

Plasmid

#26231

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

LexA::p65 (RELA Human)

Expression

Insect

Available Since

Oct. 7, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-EF1a-EGFP-RAB5A-IRES-Puromycin

Plasmid

#134858

Purpose

Expresses EGFP-tagged early endosome marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Ras-related protein 5A

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQAT47

Plasmid

#223419

Purpose

T-DNA vector for Mb2Cas12a-RVRR based mutagenesis for monocot plants; more relaxed PAM requirements; Mb2Cas12a-RVRR and the crRNA were driven by separate ZmUbi1; Hygromycin for plants selection.

Depositor

Yiping Qi

Article

Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

Insert

ZmUbi-Mb2Cas12a-RVRR-ZmUbi-crRNA scaffold

Use

CRISPR

Tags

NLS

Expression

Plant

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pTriEx-mCherry-LOV2 V416L

Plasmid

#81036

Purpose

mammalian expression of LOV V416L, a mutant with super long relaxation time

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV V416L

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

V416L

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
TrkB mEGFP

Plasmid

#83952

Purpose

FRET donor for dynamically measuring TrkB activity in neurons

Depositor

Ryohei Yasuda

Article

Harward et al Nature. 2016 Sep 28;538(7623):99-103. do

Insert

Tropomyosin related kinase B (Ntrk2 Mouse)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV BAF170-Flag

Plasmid

#19142

Depositor

Joan Massague

Article

Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

Insert

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 2 (SMARCC2 Human)

Tags

Flag

Expression

Mammalian

Available Since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pBPnlsLexA::p65Uw

Plasmid

#26230

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

nlsLexA::p65 (RELA Human)

Expression

Insect

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
miReporter-PGK

Plasmid

#82477

Purpose

microRNA reporter relying on a bidirectional PGK promoter. Contains MCS downstream of H2B-mCherry and H2B-Citrine to insert miRNA binding sites. Contains a Hygromycin selection cassette.

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2016 May 6;11(5):e0155177. doi

Insert

H2B-mCherry and H2B-Citrine. HygroR

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pYFAC-ubi-Y-pyrG

Plasmid

#184497

Purpose

AMA1 based vector for optimised episomal expression in filamentous fungi, with increased selection stringency using the modified ubi-Y-pyrG marker (high stringency).

Depositor

Yit Heng Chooi

Article

Roux et al Microb Biotechnol. 2024 Sep;17(9):e70010

Type

Empty backbone

Use

Synthetic Biology; Expression in aspergillus and …

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCdrA-gfpC

Plasmid

#111614

Purpose

Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

Depositor

Tim Tolker-Nielsen

Article

Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

Insert

PcdrA-RBSII-gfp(Mut3)-T0-T1

Expression

Bacterial

Available Since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Cp PS Intein tTA

Plasmid

#242026

Purpose

Blue-light-controlled release of tTA transactivator from the cytoplasm to the nucleus.

Depositor

Vladislav Verkhusha

Article

Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

Insert

Cp PS Intein tTA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
siRNA-resistant mNG-ORP8

Plasmid

#195170

Purpose

mammalian expression of siRNA-resistant ORP8 tagged with mNeonGreen

Depositor

Gia Voeltz

Article

Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

Insert

ORP8 (OSBPL8 Human)

Tags

mNeonGreen

Expression

Mammalian

Mutation

Wobble mutations for siRNA resistance from K174 t…

Promoter

CMV

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
LRP6 deltaN-pCS2-VSVG

Plasmid

#27281

Depositor

Xi He

Article

Tamai et al Mol Cell. 2004 Jan 16. 13(1):149-56.

Insert

low density lipoprotein receptor-related protein 6 (LRP6 Human)

Use

Xenopus injection

Tags

VSVG

Mutation

contains amino acid residues 1370–1613 (extracell…

Available Since

Jan. 26, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV2-hNCOA6:V5

Plasmid

#63585

Purpose

A subunit of the Trithorax-related (Trr) methyltransferase complex.

Depositor

Kenneth Irvine

Article

Oh et al Cell Rep. 2014 Jul 24;8(2):449-59. doi:

Insert

NCOA6 (NCOA6 Human)

Tags

6xHis and V5

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only
pHAGE-EF1alphaL-hAAT-W

Plasmid

#24527

Purpose

Lentiviral overexpression

Depositor

Darrell Kotton

Article

Wilson et al J Clin Invest. 2010 Jan . 120(1):379-89.

Insert

alpha-1 antitrypsin (SERPINA1 Human)

Use

Lentiviral

Available Since

April 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pAurora

Plasmid

#190158

Purpose

Regulates bacterial expression of target gene in blue-light-activated manner. Based on Nakamurella multipartita PAL.

Depositor

Andreas Moeglich

Article

Ranzani et al ACS Synth Biol. 2022 Oct 21;11(10):3482-

Type

Empty backbone

Tags

His6 tag

Expression

Bacterial

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
YFP-Rac1

Plasmid

#11391

Depositor

Joel Swanson

Article

Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

Insert

ras-related C3 botulinum toxin substrate 1 (RAC1 Human)

Tags

YFP

Expression

Mammalian

Available Since

Feb. 21, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-NRF1-HA

Plasmid

#34708

Depositor

Raymond Deshaies

Article

Radhakrishnan et al Mol Cell. 2010 Apr 9;38(1):17-28.

Insert

NRF1 (NFE2L1 Human)

Use

Retroviral

Tags

3XFlag and HA

Expression

Mammalian

Promoter

LTR

Available Since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag pLJM1 RagB 99L

Plasmid

#19315

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding B (RRAGB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Q99L

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge

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